... to finish creating the synthetic bacterial genome using a method called
recombination (a process that cells naturally use to repair chromosome ... were inserted into yeast cells in one step and through the mechanism of
recombination were assembled into the synthetic M. genitalium genome. ...
... years of work perfecting
chemical assembly, the team found they could use
(a process that cells use to repair damage to their ... well, and that they were able to assemble the
fragments together by
recombination. This process was used to
assemble the last cassettes, from ...
... be held no
later than October 30, 2008.
About the licensed patents:
The agreement relates to Institut Pasteur's patents covering certain
recombination (US patents #6 528 313, 6 638 768 and 6
528 314, Japanese patents #3059481, #3298842 and #3298864 and European
patents EP #419 621 and ...
... of the cell and lead to the creation of a cancerous cell.
One major DNA-repair mechanism involved in repairing these breaks is known as
recombination. This mechanism has been observed for the first time by Delft University of Technology researchers in real time and at the level of a ...
... other nucleic acid systems and their interaction with proteins and enzymes.
The Holliday junction is a four-stranded DNA structure that forms during
recombination for example, when damaged DNA is repaired. The junction is named after geneticist Robin Holliday, who proposed the model of DNA-strand ...
... of both the introduced
gene and the homologous transcript is made, but that
it is ... was sufficient to completely silence the
homologous gene's expression. Furthermore, injection of ...
The GeneZoo or the GeneJungle?... or
Zinc finger protein homologous to Zfp-36 in mouse (ZFP36).
... match strategy to help locate clones having homologous sequences
in the UniGene database. Even if ... an indirect database with a
link to the homologous Stratagene clone. When you perform sequence ...
Generate Adenovirus Vectors in E. coli by Homologous Recombination
with the AdEasy Adenoviral Vector System... then transferred
into the adenoviral genome by homologous recombination in E. coli. This
method allows ... the gene into the adenovirus genome by
means of homologous recombination in vivo. 2 To isolate and ... original E1 region of the adenovirus genome by homologous recombination.
The pAdEasy-1 vector ...
Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)... 6 ), determination of bacterial survival rates 7 or measurement of homologous DNA recombination repair which plays a role in cell viability/genomic ... R.J. & Taylor, J.A. (2001). A novel host cell reactivation assay to assess homologous recombination capacity in human cancer cell lines. Biochem. Biophys. Res. ...
Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A... of drugs and vaccines (Johnston et al. 1999).
Gene transfer in homologous systems has been shown to be a powerful tool
for the functional ... presented here, we show a practical
way to study genes in a homologous system. This method makes it possible
to characterize ...
Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae... in chromosome length observed for
otherwise apparently homologous chromosomes; 3) the possibility of translocations
or deletions ... and 4) the possibility of sequence
heterology of functionally homologous genes resulting in differential
hybridization. This has been ...
The Bio-Rad Silver Stain, Rev E... range 0.052 ng/mm 2
Quantitative comparisons limited to homologous proteins
Optical density vs. protein concentration plot ... and muscle nicotinic acetylcholine
receptors are different but homologous proteins, Proc Natl Acad Sci USA
82, 52085212 (1985)
Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms... (GC-clamped) primer. For this region, we substituted
the homologous sequence from Drosophila yakuba inferred from a manual
(EPIC) amplification. 7 When the size of a homologous intron varies among
species, one may analyze the smaller ...
Nuclease Protection Assays: The Basics... information on transcript size. The portion of probe
homologous to target RNA determines the size of the protected
... antisense probe must typically be completely
homologous to target RNA to prevent cleavage of the
MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers... mice . The sequences of
many of the miRNAs are homologous between species, suggesting
that miRNAs represent an ... In animals, miRNAs are partially
homologous to their mRNA targets. Using similar target
The use of TaqMaster PCR Enhancer... stem cells
are screened for the integration of new DNA through homologous recombination.
ES cells that are positive for a homologous recombination event are then
transplanted into blastocyst stage ...
Transgenic Animals... in vitro into these cells. The target of
this process is homologous recombination with the chromosome of
the cell, i.e. introduction or exchange of DNA at one location with
homologous DNA sequences. Following transfection, the descendants
Efficiently Insert Unique Restriction Sites into Plasmid Vectors... restriction enzyme recognition sites into the plasmid vector,
homologous oligonucleotides (49 bases long) were constructed ( Figure
1 ). ... 5
terminal bases and eleven, 3 terminal bases
homologous to the 6-kb vector. The 28 interior nucleotides destined for
Safe Production of High-Titer Retrovirus... of production of
replication-competent retrovirus (RCR) due to homologous recombination between
the vectors. 1
Although the relative speed ... probability of generating
replication-competent retrovirus (RCR) by homologous recombination is
Compatibility: Compatible ...
UW-Madison scientists find a key to cell division... are cheaper and quicker to use than mammalian cells, to assess gene function," Skop says. "All but two of the proteins from the mammalian cells were homologous in the nematodes, which allowed us to perform this mutli-organismal approach. The fact that the process is highly conserved across two very different ...
Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System... re-absorption, insulin secretion, platelet aggregation, and neurotransmitter release at sympathetic nerve endings (1). They consist of three highly homologous subtypes encoded by distinct genes: α 2a , α 2b , and α 2c . Both α 2a and α 2c subtypes are required for normal ...
Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA... (RNAi) is the
phenomenon by which double-stranded RNA (dsRNA) specifically
suppresses the expression of a gene with homologous sequence at the
post-transcriptional level. In C. elegans, D. melanogaster
and mammalian cell cultures this effect ...
Fast, Accurate Assessment of siRNA-induced Gene Silencing...
qRT-PCR with TaqMan Probes
TaqMan Probes are short
oligonucleotides that are homologous to an internal region of
the PCR product and are labeled with a 5' fluorophore and 3'
Highly Sensitive microRNA Array Performance... with probes targeting 40 different miRNAs. Each
of the seven arrays included a different miR-16 probe--one was completely
homologous to miR-16, two had distinct single mismatches, two had double
mismatches, and two had three mismatches. miRNA from human tissue was
Inducing RNAi with siRNA Cocktails Generated by RNase III... 3' dinucleotide overhangs with 5'-phosphate
and 3'-hydroxyl termini. These siRNAs mediate degradation of
homologous transcripts, leading to gene silencing (2-5; see
... RNA extraction kits on RNA quality was investigated. RNA was
tested for quality and approximate yield, as well as the reproducibility
sample replicates. The data revealed clear differences
between the different isolation kits and also between the staining
procedures that were ...
RNAi is a well-conserved cellular
mechanism believed to have antiretroviral
effects. dsRNA fragments
sequence to a
translated mRNA fragment will
effectively silence the expression
of the protein by catalyzing the
degradation of the ...
Mapping and sequencing whole genomes
Mapping intron/exon structures by amplifying cDNAs
Characterizing cloned sequences carried in lambda-phage
Amplifying full-length cDNAs or long fragments ...
... of functional genomics. In RNAi, genespecific inhibition is mediated
double-stranded RNA (dsRNA) that has a short sequence
to a region
of the mRNA transcribed from the target gene. The ability to simply, effectively,
and specifically down-regulate the ...
... one aliquot of each clone and
use another for DNA preparation to identify correctly transfected clones
recombination by PCR or Southern Blot analysis.
Detection methods for transfection:
... in transgenic mice technology and may be useful for
detailed analysis of chromosomal loci with extensive modifications, either
recombination or naturally occurring (i.e.,
deletions and insertions).
Abbott, C., et al. (1988) Trends Genet. 4: ...
... eukaryotic gene is a
necessary task in the post genome era. Results of the yeast genome sequencing
project indicate that S. cerevisiae shows
one-third of the human disease genes discovered to date. 1 Hence, the
yeast makes an attractive model for studying these genes. ...