J. Craig Venter Institute Researchers Publish Significant Advance in Genome Assembly Technology

... to finish creating the synthetic bacterial genome using a method called homologous recombination (a process that cells naturally use to repair chromosome ... were inserted into yeast cells in one step and through the mechanism of homologous recombination were assembled into the synthetic M. genitalium genome. ...

Venter Institute Scientists Create First Synthetic Bacterial Genome

... years of work perfecting chemical assembly, the team found they could use homologous recombination (a process that cells use to repair damage to their ... well, and that they were able to assemble the fragments together by homologous recombination. This process was used to assemble the last cassettes, from ...

Cellectis and Regeneron Sign an Agreement Over the use of Homologous Recombination Technology

... be held no later than October 30, 2008. About the licensed patents: The agreement relates to Institut Pasteur's patents covering certain uses of homologous recombination (US patents #6 528 313, 6 638 768 and 6 528 314, Japanese patents #3059481, #3298842 and #3298864 and European patents EP #419 621 and ...

Real-time observation of the DNA-repair mechanism

... of the cell and lead to the creation of a cancerous cell. One major DNA-repair mechanism involved in repairing these breaks is known as homologous recombination. This mechanism has been observed for the first time by Delft University of Technology researchers in real time and at the level of a ...

New force-fluorescence device measures motion previously undetectable

... other nucleic acid systems and their interaction with proteins and enzymes. The Holliday junction is a four-stranded DNA structure that forms during homologous recombination for example, when damaged DNA is repaired. The junction is named after geneticist Robin Holliday, who proposed the model of DNA-strand ...

RNA Interference and Gene Silencing: History and Overview

... of both the introduced gene and the homologous endogenous gene was suppressed (homologous transcript is made, but that it is ... was sufficient to completely silence the homologous gene's expression. Furthermore, injection of ...

The GeneZoo or the GeneJungle?

... or Zinc finger protein homologous to Zfp-36 in mouse (ZFP36). ... match strategy to help locate clones having homologous sequences in the UniGene database. Even if ... an indirect database with a link to the homologous Stratagene clone. When you perform sequence ...

Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

... then transferred into the adenoviral genome by homologous recombination in E. coli. This method allows ... the gene into the adenovirus genome by means of homologous recombination in vivo. 2 To isolate and ... original E1 region of the adenovirus genome by homologous recombination. The pAdEasy-1 vector ...

Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)

... 6 ), determination of bacterial survival rates 7 or measurement of homologous DNA recombination repair which plays a role in cell viability/genomic ... R.J. & Taylor, J.A. (2001). A novel host cell reactivation assay to assess homologous recombination capacity in human cancer cell lines. Biochem. Biophys. Res. ...

Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A

... of drugs and vaccines (Johnston et al. 1999). Gene transfer in homologous systems has been shown to be a powerful tool for the functional ... presented here, we show a practical way to study genes in a homologous system. This method makes it possible to characterize ...

Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae

... in chromosome length observed for otherwise apparently homologous chromosomes; 3) the possibility of translocations or deletions ... and 4) the possibility of sequence heterology of functionally homologous genes resulting in differential hybridization. This has been ...

The Bio-Rad Silver Stain, Rev E

... range 0.052 ng/mm 2 Quantitative comparisons limited to homologous proteins Optical density vs. protein concentration plot ... and muscle nicotinic acetylcholine receptors are different but homologous proteins, Proc Natl Acad Sci USA 82, 52085212 (1985) ...

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

... (GC-clamped) primer. For this region, we substituted the homologous sequence from Drosophila yakuba inferred from a manual ... intron-crossing (EPIC) amplification. 7 When the size of a homologous intron varies among species, one may analyze the smaller ...

Nuclease Protection Assays: The Basics

... information on transcript size. The portion of probe homologous to target RNA determines the size of the protected ... antisense probe must typically be completely homologous to target RNA to prevent cleavage of the probe:target ...

MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers

... mice [10]. The sequences of many of the miRNAs are homologous between species, suggesting that miRNAs represent an ... In animals, miRNAs are partially homologous to their mRNA targets. Using similar target selection ...

The use of TaqMaster PCR Enhancer

... stem cells are screened for the integration of new DNA through homologous recombination. ES cells that are positive for a homologous recombination event are then transplanted into blastocyst stage ...

Transgenic Animals

... in vitro into these cells. The target of this process is homologous recombination with the chromosome of the cell, i.e. introduction or exchange of DNA at one location with homologous DNA sequences. Following transfection, the descendants of ...

Efficiently Insert Unique Restriction Sites into Plasmid Vectors

... restriction enzyme recognition sites into the plasmid vector, homologous oligonucleotides (49 bases long) were constructed ( Figure 1 ). ... 5 terminal bases and eleven, 3 terminal bases homologous to the 6-kb vector. The 28 interior nucleotides destined for ...

Safe Production of High-Titer Retrovirus

... of production of replication-competent retrovirus (RCR) due to homologous recombination between the vectors. 1 Although the relative speed ... probability of generating replication-competent retrovirus (RCR) by homologous recombination is greatly reduced. Compatibility: Compatible ...

UW-Madison scientists find a key to cell division

... are cheaper and quicker to use than mammalian cells, to assess gene function," Skop says. "All but two of the proteins from the mammalian cells were homologous in the nematodes, which allowed us to perform this mutli-organismal approach. The fact that the process is highly conserved across two very different ...

Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System

... re-absorption, insulin secretion, platelet aggregation, and neurotransmitter release at sympathetic nerve endings (1). They consist of three highly homologous subtypes encoded by distinct genes: α 2a , α 2b , and α 2c . Both α 2a and α 2c subtypes are required for normal ...

Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA

... (RNAi) is the phenomenon by which double-stranded RNA (dsRNA) specifically suppresses the expression of a gene with homologous sequence at the post-transcriptional level. In C. elegans, D. melanogaster and mammalian cell cultures this effect ...

Fast, Accurate Assessment of siRNA-induced Gene Silencing

... qRT-PCR with TaqMan Probes TaqMan Probes are short oligonucleotides that are homologous to an internal region of the PCR product and are labeled with a 5' fluorophore and 3' quencher. ...

Highly Sensitive microRNA Array Performance

... with probes targeting 40 different miRNAs. Each of the seven arrays included a different miR-16 probe--one was completely homologous to miR-16, two had distinct single mismatches, two had double mismatches, and two had three mismatches. miRNA from human tissue was ...

Inducing RNAi with siRNA Cocktails Generated by RNase III

... 3' dinucleotide overhangs with 5'-phosphate and 3'-hydroxyl termini. These siRNAs mediate degradation of homologous transcripts, leading to gene silencing (2-5; see also "

Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.

... RNA extraction kits on RNA quality was investigated. RNA was tested for quality and approximate yield, as well as the reproducibility within homologous sample replicates. The data revealed clear differences between the different isolation kits and also between the staining procedures that were ...

siRNA transfection optimization with the Agilent 2100 bioanalyzer

... therapeutics. RNAi is a well-conserved cellular mechanism believed to have antiretroviral effects. dsRNA fragments with homologous sequence to a translated mRNA fragment will effectively silence the expression of the protein by catalyzing the degradation of the ...

Expand Long Template PCR System

... Mapping and sequencing whole genomes Mapping intron/exon structures by amplifying cDNAs Analyzing homologous recombination events Characterizing cloned sequences carried in lambda-phage Amplifying full-length cDNAs or long fragments ...

QIAGEN Instrument Service insist on the best in service and support

... of functional genomics. In RNAi, genespecific inhibition is mediated by short double-stranded RNA (dsRNA) that has a short sequence homologous to a region of the mRNA transcribed from the target gene. The ability to simply, effectively, and specifically down-regulate the ...

Embryonic stem cells, mouse

... one aliquot of each clone and use another for DNA preparation to identify correctly transfected clones originating from homologous recombination by PCR or Southern Blot analysis. Detection methods for transfection: ...

Easily Amplify Genomic DNA with Long-Distance PCR

... in transgenic mice technology and may be useful for detailed analysis of chromosomal loci with extensive modifications, either introduced by homologous recombination or naturally occurring (i.e., deletions and insertions). REFERENCES Abbott, C., et al. (1988) Trends Genet. 4: ...

Epitope-Tagging Vectors for Functional Analysis in Yeast

... eukaryotic gene is a necessary task in the post genome era. Results of the yeast genome sequencing project indicate that S. cerevisiae shows homologous sequences for one-third of the human disease genes discovered to date. 1 Hence, the yeast makes an attractive model for studying these genes. ...