SeeSNP Propietary Analysis Advantages
... when the artifact is present in a heterozygote
When forward and reverse ... generate higher quality base-calls than heterozygote
the quality scores of the heterozygote
PHD files such that
PolyPhreds source ...
Genotyping Single Nucleotide Polymorphisms with Molecular Beacons
... homozygote (A/A), mismatched probe-target
homozygote (G/G), and heterozygote
(A/G) for the SNP.
The fluorescence of the ... clearly differentiate a homozygote from a
heterozygote. For example, one heterozygote
had Cts of approximately 31 with
both molecular beacons, and the Ct ...
Brilliant Core Reagent Kits for QPCR and QRT-PCR
... wild-type and the mutant allele) is detected. The Ct values for the heterozygote
were 27 (TET) and 27 (FAM) ( Figure
... the mutant sample had a FAM signal
of 1.34, and the heterozygote
showed approximately one-half of the TET
or the FAM signal.
Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
... distinctly different melting temperatures of the two possible hybrids
allow identification of each unknown sample as mutant, wild type, or heterozygote
(one allele of mutant and one allele of wild-type sequence).
Primer and Probe Design
Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
... DNA template showed only VIC fluorescence,
the AA homozygote control DNA template showed only FAM fluorescence, and
the GA heterozygote
control DNA template showed both FAM and VIC fluorescence
(Figure 1). The absence of carryover of FAM signal into channel 2 for
Mutation detection for the
K- rasand P16 genes
position 356, two extra bands
could be seen. The extra bands
detected here are due to the slower
mobility of the heteroduplex
formed by heterozygote
the samples. The detection on the
Agilent 2100 bioanalyzer are
completely matched with ...
QIAGEN Multiplex PCR Handbook
... locus. The primers were designed to generate PCR products of different sizes
so that wild-type mice (wt) could be easily distinguished from heterozygote
mutant mice (ht), or homozygote mutant mice (hm).
Analysis of microsatellites
Eukaryotic genomes ...