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Heterozygote in Biological Technology

SeeSNP Propietary Analysis Advantages

... when the artifact is present in a heterozygote base-call. When forward and reverse ... generate higher quality base-calls than heterozygote base-calls (Figure 2). ... inflates the quality scores of the heterozygote PHD files such that PolyPhreds source ...

Genotyping Single Nucleotide Polymorphisms with Molecular Beacons

... homozygote (A/A), mismatched probe-target homozygote (G/G), and heterozygote (A/G) for the SNP. The fluorescence of the ... clearly differentiate a homozygote from a heterozygote. For example, one heterozygote had Cts of approximately 31 with both molecular beacons, and the Ct ...

Brilliant Core Reagent Kits for QPCR and QRT-PCR

... wild-type and the mutant allele) is detected. The Ct values for the heterozygote were 27 (TET) and 27 (FAM) ( Figure 3 ). ... the mutant sample had a FAM signal of 1.34, and the heterozygote showed approximately one-half of the TET or the FAM signal. ...

Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A

... distinctly different melting temperatures of the two possible hybrids allow identification of each unknown sample as mutant, wild type, or heterozygote (one allele of mutant and one allele of wild-type sequence). Methods Primer and Probe Design ...

Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System

... DNA template showed only VIC fluorescence, the AA homozygote control DNA template showed only FAM fluorescence, and the GA heterozygote control DNA template showed both FAM and VIC fluorescence (Figure 1). The absence of carryover of FAM signal into channel 2 for ...

Mutation detection for the K- rasand P16 genes

... at position 356, two extra bands could be seen. The extra bands detected here are due to the slower mobility of the heteroduplex formed by heterozygote mutant of the samples. The detection on the Agilent 2100 bioanalyzer are completely matched with ...

QIAGEN Multiplex PCR Handbook

... locus. The primers were designed to generate PCR products of different sizes so that wild-type mice (wt) could be easily distinguished from heterozygote mutant mice (ht), or homozygote mutant mice (hm). Analysis of microsatellites Eukaryotic genomes ...
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