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Heteroduplex in Biological Technology

Pathway Diagnostics Announces Patent Issuance for the Diagnostic Measurement of HIV Tropism for the Initiation or Modification of HIV Therapy

... assay is a proprietary application of molecular hybridization based on heteroduplex tracking technology developed by Dr. Barbara Weiser and Dr. Harold ... at the Wadsworth Center, New York State Department of Health, Albany NY. heteroduplex tracking technology interrogates the complete HIV V3 loop, detecting ...

Heteroduplex Analysis Using the DCode System

... Introduction heteroduplex Analysis (HA) is a commonly used mutation ... in double-stranded DNA caused by the formation of heteroduplex molecules. 1 heteroduplex molecules have a mismatch in the double-strand, ...

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

... Secondly, the deliberate construction of heteroduplex molecules by pooling homoduplex PCR products often yields unique heteroduplex electrophoresis patterns, enabling ... final extension at 72 C. heteroduplex DNA was formed by pairwise pooling of 10 l ...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

... C and reannealed for 1 hour at 55 C to allow heteroduplex formation. Melting temperature of the ... positive control, and demonstrates the heteroduplex bands formed by polymorphic and wild ... 4, 5, 7, 9, and 10 demonstrate heteroduplex band formation, indicating an alteration in ...

DGGE Analysis Using the DCode System, Rev B

... in lanes 13 were resolved into two heteroduplex and two homoduplex bands. The heteroduplex bands (upper bands) migrated slower ... samples. The ability to resolve the heteroduplex fragments and the mutant homoduplex ...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

... (CDGE), 3 temperature gradient gel electrophoresis (TGGE), 4 heteroduplex analysis (HA), 5 protein truncation test (PTT), 6 and ... or 1.5x improved the resolution of the homoduplex bands and heteroduplex bands, thus making it easier to identify mutants from wild-type ...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

... differential melting characteristics of homoduplex DNA versus heteroduplex DNA. As heteroduplex DNA migrates through the denaturant ... reduction in mobility results in a separation of homoduplex from heteroduplex fragments thereby identifying a region of sequence ...

Denaturing HPLC (DHPLC)

... reversed-phase high-performance liquid chromatography (IP-RP-HPLC) technique. It identifies mutations and polymorphisms based on detection of heteroduplex formation between mismatched nucleotides in double-stranded PCR amplified DNA. Sequence variation creates a mixed population of heteroduplexes and ...

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

... programmed to denature the amplification products at 96 C for 8 minutes and to then perform slow cooling to 40 C. Without this last step, heteroduplex DNA may not be visible on the DGGE gels. In the case of apoA-I, a 420 bp region at the 5' end of the gene, containing ...

Detection of Mutations in the CYP21 Gene Using the DCode System

... C for 10 minutes to denature the PCR products and subsequently left at room temperature for gradual renaturation, thereby generating heteroduplex molecules. Denaturing Gradient Gel Electrophoresis Denaturing gradient gel electrophoresis was carried out using ...

RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes

... experiments unrelated to mismatch repair. Mismatched base pairs in DNA can form in vivo through processes such as replication errors, heteroduplex formation, deamination of 5-methylcytosine and the presence of DNA adducts. 1 Cells cope with mismatches by enlisting specialized proteins that ...
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