Pathway Diagnostics Announces Patent Issuance for the Diagnostic Measurement of HIV Tropism for the Initiation or Modification of HIV Therapy
... assay is a proprietary application of molecular
hybridization based on heteroduplex
tracking technology developed by Dr.
Barbara Weiser and Dr. Harold ... at the Wadsworth Center,
New York State Department of Health, Albany NY. heteroduplex
technology interrogates the complete HIV V3 loop, detecting ...
Heteroduplex Analysis Using the DCode System
Analysis (HA) is a commonly used mutation ... in double-stranded DNA caused by the formation of heteroduplex
molecules have a mismatch in the double-strand, ...
Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
... Secondly, the deliberate construction of heteroduplex
molecules by pooling
homoduplex PCR products often yields unique heteroduplex
patterns, enabling ... final extension
at 72 C.
DNA was formed by pairwise pooling of 10 l ...
Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis
... C and reannealed for 1 hour at 55 C to allow heteroduplex
Melting temperature of the ... positive control, and
demonstrates the heteroduplex
bands formed by polymorphic
and wild ... 4, 5, 7, 9, and 10 demonstrate
band formation, indicating an alteration in ...
DGGE Analysis Using the DCode System, Rev B
... in lanes 13 were resolved into two
and two homoduplex bands. The heteroduplex
bands (upper bands)
migrated slower ... samples. The
ability to resolve the heteroduplex
fragments and the mutant homoduplex
Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations
... (CDGE), 3
temperature gradient gel electrophoresis (TGGE), 4 heteroduplex
(HA), 5 protein truncation test (PTT), 6 and ... or 1.5x
improved the resolution of the homoduplex bands and heteroduplex
thus making it easier to identify mutants from wild-type ...
A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
... differential melting characteristics of
homoduplex DNA versus heteroduplex
DNA. As heteroduplex
DNA migrates through the denaturant ... reduction in mobility results in a
separation of homoduplex from heteroduplex
thereby identifying a region of sequence ...
Denaturing HPLC (DHPLC)
... reversed-phase high-performance liquid chromatography (IP-RP-HPLC) technique.
It identifies mutations and polymorphisms based on detection of heteroduplex
formation between mismatched nucleotides in double-stranded PCR amplified DNA. Sequence variation creates a mixed population of heteroduplexes and ...
Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System
... programmed to denature the amplification products at 96 C for 8 minutes
and to then perform slow cooling to 40 C. Without this last step, heteroduplex
DNA may not be visible on the DGGE gels.
In the case of apoA-I, a 420 bp region at the 5' end of the gene, containing
Detection of Mutations in the CYP21 Gene Using the DCode System
... C for 10 minutes to denature the PCR products and subsequently left at
room temperature for gradual renaturation, thereby generating heteroduplex
Denaturing Gradient Gel Electrophoresis
Denaturing gradient gel electrophoresis was carried out using ...
RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
... experiments unrelated to mismatch repair.
Mismatched base pairs in DNA can form in vivo through processes such as
replication errors, heteroduplex
formation, deamination of 5-methylcytosine and
the presence of DNA adducts. 1 Cells cope with mismatches by enlisting
specialized proteins that ...