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Gradient in Biological Technology

Carbon nanotubes made into conductive, flexible 'stained glass'

... alternative transparent conductor. Utilizing a technique known as density gradient ultracentrifugation, the researchers have produced carbon nanotubes with ... to pre-existing carbon nanotube materials. In addition, density gradient ultracentrifugation allows carbon nanotubes to be sorted by their optical ...

Model predicts evolution of Mediterranean landscape following fires

... automata to represent fire "We used 'cellular automata' to represent how fires spread, bearing in mind the flammability of the ground cover, the gradient of the land, wind speed and moisture levels of the vegetation", the lead author of the study, James D. A. Millington, from Michigan State University ...

STAAR Surgical Releases Afinity(TM) Collamer(R) Aspheric Single Piece NTIOL

... ability. "Our patented Collamer material has similarities with the eye's natural crystalline lens. Collamer has high water content and a gradient refractive index, much like the natural lens, providing for its superior anti-glare characteristics. It is also highly compressible which allows the ...

Method sorts out double-walled carbon nanotube problem

... now offer a clever solution to the double-walled nanotube production problem. They used a technique developed at Northwestern called density gradient ultracentrifugation to cleanly and easily separate the double-walled nanotubes (DWNTs) from the single-walled nanotubes (SWNTs) and multi-walled ...

VAP(R) Cholesterol Test Helps Researchers Identify Treatment Effects of Combination Therapy

... (IDL), LDL and HDL subfractions was quantified using the Vertical Auto Profile method. LDL particle size was determined using segmented gradient gel electrophoresis (S3GGE(TM) Berkeley Heart Lab). A key feature of the comprehensive VAP Test is the identification of the major lipid components ...

New map IDs the core of the human brain

... used state-of-the-art diffusion MRI technology, which is a non-invasive scanning technique that estimates fiber connection trajectories based on gradient maps of the diffusion of water molecules through brain tissue. A highly sensitive variant of the method, called diffusion spectrum imaging (DSI), can ...

New Chromatography Detectors From Varian, Inc. Extend the Range of Universal Detection for HPLC and LC/MS

... of two new evaporative light scattering detectors (ELSD) for conventional and high speed liquid chromatography. With their universal response, full gradient and solvent compatibility, and excellent baseline stability, Varian ELSDs possess key advantages over conventional UV and RI detectors for busy ...

Life at the jolt

... the flow of electrons. The treatment of the biofilm matrix as a conductor allowed the team to describe the transport of electrons driven by the gradient in the electrical potential. The relationship between the biofilm matrix and the anode could now be described by a standard equation for an ...

Fluid theory confirmed by Foton

... scientists discovered that these tiny fluctuations in fluids and gases can increase in size, and even be made visible to the naked eye, if a strong gradient is introduced. One way to achieve this is to increase the temperature at the bottom of a thin liquid layer, though not quite enough to cause ...

BioNanomatrix Announces Issuance of Key Nanofluidics Patent Enabling Single Molecule Whole Genome Analysis

... a simple approach that allows the construction of hundreds of thousands of enclosed, parallel nanofluidic channels on a single surface, using a gradient structured interface to gradually guide the biologically active molecules into the nanoscale channels. This simple technique is ideally suited for ...

Prominence UFLC

... in flow path pressure (autosampler), high-speed gradient (controller and solvent delivery units), and ... and peak area of 7 alkyl phenones under steep gradient conditions and a 1-minute run time. Prominence ... the column, causing a temperature gradient within the columns cross section. ...

Speciation of Arsenic by LC-ICP-MS

... can be performed using either an isocratic or gradient LC method (see Figures 2 and 3). Using either ... is 0.01 μg/L (10 ng/L). While the gradient method increases the analysis time by ... peak (75ArCl). As shown in Figure 5, the gradient method adequately separates the Cl peak from the ...

2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ

... and eluted at 100 µl/min with a linear salt gradient of 0–30% B over 40 min (A: 20 mM citric ... analytical column at 250 nl/min with a linear gradient of 0–60% B over 50 min (A: 0.1% formic ... digest was first separated by SCX using salt gradient elution. The chromatogram is shown in Figure 2. ...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

... of gene fragments that, when evaluated by density gradient gel electrophoresis (DGGE), produced ... and L. seeligeri . 5 Density gradient gel electrophoresis (DGGE) can be used to ... can be controlled by establishing a gradient of DNA denaturants such as urea and formamide ...

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

... diseases. We have used the denaturing gradient gel electrophoresis method (DGGE) 1 using the ... the percentage range of the denaturing gradient to be used in the gels. Multiple aliquots of ... epi-illumination at 254 nm. 4 A 3565% denaturing gradient was used for apoA-I and a ...

Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis

... the use of Temporal Temperature gradient Gel Electrophoresis (TTGE) as the method of ... studies. In the commonly used Denaturing gradient Gel Electrophoresis, 5 ... by using TTGE, one avoids pouring chemical gradient gels. 6 Temporal ...

Detection of Mutations in the CYP21 Gene Using the DCode System

... a complicated matter. We have used the denaturing gradient gel electrophoresis method (DGGE) ... molecules. Denaturing gradient Gel Electrophoresis Denaturing gradient gel electrophoresis was carried out using the ...

Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A

... cell was used to run up to 11 vertical 916% gradient SDS-PAGE gels. Comparison of the gel ... chamber, and the Model 395 gradient former. (The Model 495 gradient former, catalog #165-4121, is now ...

The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B

... under the presence of a mismatch. The gradient The gradient feature was developed to be used with the Bio-Rad iCycler iQ system. The gradient allows evaluation of up to eight annealing, ...

Enzyme Purification With the Econo-Pac Q Cartridge

... than the user-packed column. (Note: The two gradient profiles are slightly different.) ... are loaded onto a column, and the gradient is initiated. Fractions of 4.7 ml were ... are loaded onto a cartridge, and the gradient is initiated. FPLC and Sephadex are ...

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

... in wild or domesticated populations. Denaturing gradient gel electrophoresis using the DCode ... two tautog PCR products, perpendicular gradient denaturing gels were run to determine their ... (not shown) suggested a parallel gradient range of 40% to 60% denaturant for the cyt b ...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

... conformation polymorphism (SSCP), 1 denaturing gradient gel electrophoresis (DGGE), 2 ... electrophoresis (CDGE), 3 temperature gradient gel electrophoresis (TGGE), 4 heteroduplex ... new technique called temporal temperature gradient gel electrophoresis (TTGE). ...

Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System

... exon of HLA-DRB alleles, using denaturing gradient gel electrophoresis (DGGE). When ... established on a perpendicular denaturing gradient as described by Myers and coworkers. ... gel with an increasing gradient from 0% to 80% denaturant (100% denaturant=7 M ...

TTGE on the DCode System

... Introduction Temporal temperature gradient electrophoresis (TTGE) exploits the principles upon which denaturing gradient gel electrophoresis (DGGE) and constant ... there is no need for a chemical denaturing gradient which is the advantage in CDGE. The ...

Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A

... of PCR products by using temporal temperature gradient gel electrophoresis (TTGE). In the commonly used Denaturing gradient Gel Electrophoresis, 6 electrophoretic ... TTGE, one avoids pouring chemical gradient gels. 7 We have developed a strategy ...

Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System

... combined with Multiplex Denaturing gradient Gel electrophoresis analysis (Michiels et al., ... and MP10) or a 3080% (for MP1 and MP7) gradient (Model 475 gradient Delivery system, Bio-Rad) of urea and ...

Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System

... Introduction Denaturing gradient gel electrophoresis (DGGE) is one of the most ... high temperature in the presence of a gradient of chemical denaturant. DNA melting is ... All products were analyzed both in perpendicular gradient gels and ...

Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System

... of population markers. Denaturing gradient gel electrophoresis (DGGE) detects sequence ... and a 2 codon indel on a perpendicular gradient gel (Figure 1). Dissociation of A1 alleles ... on 4065% (7.5% acrylamide) parallel gradient gels was used to determine the run-time that ...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

... polymorphism (SSCP), and denaturing gradient gel electrophoresis (DGGE), among others. ... acrylamide gel (37.5:1) with a perpendicular gradient of 12.537.5% denaturant. ... analyses were done in a parallel gradient of 1530% denaturant at 60 C, with ...

DGGE Analysis for Non-Invasive Studies of Primate Diet

... The applications of denaturing gradient gel electrophoresis (DGGE) to the ... were first analyzed on a perpendicular denaturing gradient gel poured with Bio-Rads Model 475 gradient Former. The gel was 6.5% 37.5:1 ...

DGGE Analysis Using the DCode System, Rev B

... Introduction Denaturing gradient gel electrophoresis (DGGE) is one of several ... of uniform temperature and a linear denaturant gradient formed with urea and formamide ... of 7 M urea and 40% formamide. The denaturing gradient may be formed perpendicular or ...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

... 1993). One of these methods, denaturing gradient gel electrophoresis (DGGE), has been ... polyacrylamide gel containing a linear gradient of denaturant. As the fragment migrates through the denaturant gradient the strands begin to melt. The melting ...

Separation Reproducibility With the BioLogic Chromatography Systems

... respectively. Proteins were eluted with a linear gradient of 050% buffer B (buffer A + 1 M NaCl). ... the protein sample using an Econo gradient pump. For the BioLogic LP system, 1 ml ... standard deviation associated with the gradient slope, 4.88 0.01 mS/cm/min, indicates ...

Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A

... parameters, such as buffer pH and gradient characteristics. Furthermore, ... experiments, varying the pH or gradient characteristics, or both, for each run. It is not ... using a 50 ml, 075% buffer B (1 M NaCl) gradient for our preliminary experiments. The protein ...

QIAGEN Multiplex PCR Handbook

... Optional : If a thermal cycler with a temperature gradient function can be used, determine the ... temperature by performing a gradient PCR. Protocol ... Optional: If a thermal cycler with a temperature gradient function can be used, determine ...

Two-step Gradient PCR*

... extension of the PCR techniqueTwo-step gradient PCR. This method offers significant time-savings ... was performed using the Eppendorf Mastercycler gradient (3), which provided excellent, ... out using the Eppendorf Mastercycler gradient and Eppendorf Taq Polymerase. Nine micro liters ...

7 Programming

... 7.6.1 gradient PCR (for Mastercyclera gradient only) The gradient PCR is used to optimize temperatures in a PCR ...

PCR Optimization

... target. The Eppendorf Mastercycler gradient provides a gradient function, that in one single run ... Technology allows seamless transfer of gradient experiments to standard PCR protocols. ...

Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes

... were carried out using the Mastercycler gradient from Eppendorf. Loading buffer (Sambrook ... mM 3.5 mM) and by applying a temperature gradient (58C 70C). 20 fg Listeria ... by applying a temperature gradient (55C 65C; 3 mM MgCl2). 20 fg Listeria ...
Other Contents
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