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Fluorescein in Biological Technology

Serial Dilution with the Bravo Liquid Handling Platform

... flat clear bottom plates (Greiner 655087) fluorescein solution (3 μM in 50 mM Tris-HCl, pH 8.0) ... Method A reservoir containing 60 mL of fluorescein solution is placed on position 2 of the Bravo ... last column of the head from position 6. 5. fluorescein solution is mixed at position 2. Mix parameters ...

Phosphorescence lifetime measured using the SPEX phosphorimeter

... from a peptide-terbium-complex donor to a fluorescein acceptor. The peptide in the complex absorbs ... nm, the terbium phosphoresces at 485 nm, where fluorescein dye absorbs. The fluorescence of fluorescein can be observed when the sample is excited at 280 ...

Sub-Picomolar Fluorescein

... 1 shows spectra of 400 fM (0.4 pM or 4 1013 M) fluorescein in 0.01-N NaOH, a blank of solely 0.01-N NaOH, and the subtracted spectrumof fluorescein without the solvent. The large peak near 520 nm clearly shows the presence of fluorescein at a 0.4-pM concentration. At the highest ...

In Situ Cell Death Detection Kit

... of the TUNEL kits. Labeling with fluorescein may also be followed by ... The In Situ Cell Death Detection Kits, fluorescein and TMR red, can also be used with cells ... The In Situ Cell Death Detection Kit, fluorescein was used for staining a paraffin-embedded ...

Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)

... to compare two types of microplates for their ability to detect a 1 M fluorescein solution compared to suspension of GFP-expressing cells (Table 2). Interestingly, the fluorescein signal increased from 11190 RFU in Greiner plates to 32787 RFU in ABgene ...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

... 0.5 U of Platinum Taq DNA polymerase (Invitrogen), 10 nM fluorescein calibration dye (Bio-Rad), 1 l of a 1:1,500 dilution of 10,000x ... of iQ supermix (Bio-Rad) at a final concentration of 1x, 10 nM fluorescein calibration dye, 1 l of a 1:1,500 dilution of 10,000x SYBR ...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

... Abstract Labeling GAPDH and c-myc siRNA with either fluorescein or Cy3 was demonstrated to have no effect on silencing ... Red: Cy3 labeled siRNA; Blue: DAPI stained nuclei; Green: fluorescein labeled antibody to GAPDH. A. siRNA silencing of GAPDH ...

Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)

... with each other. If their emission spectra do not overlap, detection of one in the presence of the other is straightforward. Such is the case for fluorescein (FAM)- and tetramethylrhodamine (TAMRA)-labelled probes. Using the instrument settings intended for FAM, the TAMRA probe/ target complex does not ...

Fluorescence assay for determination of protease activity

... overnight at room temperature and then measured. DETECTION The detection was done using the factory preset settings for fluorescein label. Excitation was at 485 nm and emission was measured at 535 nm. RESULTS Measurement with the new VICTOR fluorometer gives ...

Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein

... fixation for visualization. EGFP fluoresces green when excited by light of 488 nm and may be visualized by epifluorescence microscopy using standard fluorescein isothiocyanate filters (Chalfie et al., 1994) under wavelengths that are not deleterious to living tissues. Since EGFP consists of ~200 amino acid ...

Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module

... with 5-μM Hoechst™ 33342 in PBS for 15 min at room temperature.

8. Image wells using excitation and emission settings for fluorescein and Hoechst 33342.

Analysis protocol The IN Cell Analyzer 3000 Micronuclei Formation Analysis Module ...

The iCycler iQ Detection System for TaqMan Assays, Rev B

... et al. 1991). In a fluorescent TaqMan assay, the probe is labeled at the 5' end with a fluorescent reporter molecule such as fluorescein and at the 3' end with another fluorescent molecule, usually a tetramethyl- rhodamine derivative, which acts as a quencher for ...

RNAi in Non-Mammalian Cultured Cells

... TechNotes 9(3): 68. Cy 3 is a trademark of Amersham Biosciences. The Cy3 and fluorescein Labeling reagents manufactured for Ambion by Mirus Corporation. back to top ...

More siRNA Vectors for RNA Interference

... antibody (Ambion). Green: anti-GAPDH antibody detected with fluorescein labeled secondary antibody; Blue: DAPI stained nuclei. The p Silencer 2.0-U6 and 3.0-H1 vectors each include ...

Drive Long-Term Silencing with CMV Vectors

... have a significant amount of GAPDH knocked down. Immunofluorescence was performed using gene specific primary antibodies and a fluorescein conjugated secondary antibody and the resulting reduction in protein expression was examined by fluorescence microscopy. The ...

GENE SILENCING WITH PCR PRODUCTS

... the cells were assessed using nuclear staining with DAPI and immunofluorescence using a c-FOS antibody detected with a fluorescein labeled secondary antibody. Figure 2. siRNA Expression Cassette-Mediated Reduction in GAPDH Expression. ...

Control Your siRNA Research

... into HeLa cells and cells were analyzed 96 hours later. Green: -actin protein detected with anti--actin (Ambion) and a fluorescein labeled secondary antibody. Red: Cy3 labeled siRNA. Blue: DAPI stained nuclei. (Cy3 is a trademark of Amersham Biosciences.) ...

Setting up a kinase profiler with IMAP

... AD or HT, 20,000 sec for GT Settings for the MDC SpectraMax M5: Endpoint Mode fluorescein Excitation: 485 nm Emission: 530 nm Auto cut-off: 515 nm TAMRA Excitation: ...
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