Serial Dilution with the Bravo Liquid Handling Platform
... flat clear bottom plates (Greiner 655087)
solution (3 μM in 50 mM Tris-HCl, pH 8.0) ...
Method A reservoir containing 60 mL of fluorescein
placed on position 2 of the Bravo ... last column of the head from position 6.
solution is mixed at position 2. Mix parameters ...
Phosphorescence lifetime measured using the SPEX phosphorimeter
... from a peptide-terbium-complex donor to a fluorescein
acceptor. The peptide in the complex absorbs ... nm, the terbium phosphoresces at 485 nm, where fluorescein
dye absorbs. The fluorescence of fluorescein
can be observed when the sample is excited at 280 ...
... 1 shows spectra of 400 fM (0.4 pM or 4 1013 M) fluorescein
in 0.01-N NaOH, a blank of
solely 0.01-N NaOH, and the subtracted spectrumof fluorescein
without the solvent. The large peak near 520 nm clearly shows the presence of fluorescein
at a 0.4-pM concentration.
At the highest ...
In Situ Cell Death Detection Kit
the TUNEL kits. Labeling with fluorescein
may also be followed by
... The In Situ
Cell Death Detection Kits, fluorescein
and TMR red, can also be used with
cells ... The In Situ Cell Death
Detection Kit, fluorescein
was used for staining a paraffin-embedded
Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)
... to compare two types of microplates for their ability to detect a 1 M fluorescein
solution compared to suspension of GFP-expressing cells (Table 2). Interestingly, the fluorescein
signal increased from 11190 RFU in Greiner plates to 32787 RFU in ABgene ...
Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
... 0.5 U of Platinum Taq DNA polymerase (Invitrogen),
10 nM fluorescein
calibration dye (Bio-Rad), 1 l of a 1:1,500 dilution
of 10,000x ... of iQ supermix (Bio-Rad) at a final concentration
of 1x, 10 nM fluorescein
calibration dye, 1 l of a 1:1,500 dilution of
10,000x SYBR ...
Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect
Labeling GAPDH and c-myc siRNA with either fluorescein
or Cy3 was demonstrated to have no effect on silencing ... Red: Cy3 labeled siRNA; Blue: DAPI stained
nuclei; Green: fluorescein
labeled antibody to GAPDH. A.
siRNA silencing of GAPDH ...
Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)
... with each other. If their emission spectra do not overlap, detection of one in the presence of the other is straightforward. Such is the case for fluorescein
(FAM)- and tetramethylrhodamine (TAMRA)-labelled probes. Using the instrument settings intended for FAM, the TAMRA probe/ target complex does not ...
Fluorescence assay for determination of protease activity
... overnight at room temperature and then measured.
The detection was done using the factory preset settings for fluorescein
label. Excitation was at 485 nm and emission was measured at 535 nm.
Measurement with the new VICTOR fluorometer gives ...
Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
... fixation for visualization. EGFP fluoresces green when excited by light of 488 nm and may be visualized by epifluorescence microscopy using standard fluorescein
isothiocyanate filters (Chalfie et al., 1994) under wavelengths that are not deleterious to living tissues. Since EGFP consists of ~200 amino acid ...
Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
... with 5-μM Hoechst™ 33342 in PBS for 15 min at room temperature.
8. Image wells using excitation and emission settings for fluorescein and Hoechst 33342.
The IN Cell Analyzer 3000 Micronuclei Formation Analysis Module ...
The iCycler iQ Detection System for TaqMan Assays, Rev B
... et al. 1991). In a fluorescent TaqMan
assay, the probe is labeled at the 5' end with a fluorescent reporter
molecule such as fluorescein
and at the 3' end with another fluorescent
molecule, usually a tetramethyl- rhodamine derivative, which acts as a
quencher for ...
RNAi in Non-Mammalian Cultured Cells
Cy 3 is a trademark of
The Cy3 and fluorescein
reagents manufactured for Ambion by Mirus Corporation.
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More siRNA Vectors for RNA Interference
antibody (Ambion). Green: anti-GAPDH antibody detected with fluorescein
labeled secondary antibody; Blue: DAPI stained nuclei.
The p Silencer 2.0-U6 and
3.0-H1 vectors each include ...
Drive Long-Term Silencing with CMV Vectors
... have a significant amount
of GAPDH knocked down. Immunofluorescence was performed using gene specific
primary antibodies and a fluorescein
conjugated secondary antibody and
the resulting reduction in protein expression was examined by fluorescence
microscopy. The ...
GENE SILENCING WITH PCR PRODUCTS
... the cells
were assessed using nuclear staining with DAPI and immunofluorescence
using a c-FOS antibody detected with a fluorescein
labeled secondary antibody.
Figure 2. siRNA
Expression Cassette-Mediated Reduction in GAPDH Expression. ...
Control Your siRNA Research
... into HeLa cells and cells were
analyzed 96 hours later. Green: -actin protein detected with anti--actin
(Ambion) and a fluorescein
labeled secondary antibody. Red: Cy3 labeled
siRNA. Blue: DAPI stained nuclei. (Cy3 is a trademark of Amersham Biosciences.)
Setting up a kinase profiler with IMAP
... AD or HT, 20,000 sec
Settings for the MDC SpectraMax M5:
Excitation: 485 nm
Emission: 530 nm
Auto cut-off: 515 nm