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Flank in Biological Technology

Oncolytics Biotech(R) Inc. Research Collaborators Present Glioma Reovirus Research at ASV Annual Meeting

... moderate to strong synergy in primary patient glioma cells treated with reovirus plus IR and TMZ in vitro. This effect was also seen in vivo in flank tumor xenografts from primary patient tumors resistant to reovirus therapy alone. The investigators concluded that the data supports the use of ...

Chromosome breakpoints contribute to genetic variation

... regions also have higher numbers of segmental duplications, a type of mutation that increases the copy number of genes and the sequences that flank them. "To me, this was completely counterintuitive. I thought we would have these breakpoints in gene deserts," Lewin said. "We had to rethink the ...

Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries

... were retrieved by PCR using vector-specific primers that flank the insert. The PCR products were then subcloned into ... reactions were performed using vector-specific primers, which flank the cloning site, and genomic DNA was isolated from either ...

Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers

... with wild-type (wt) sequences. The SSCP technique includes three processes: PCR amplification using primers that flank the DNA region of interest; Denaturation of the resulting double-stranded PCR product, followed by rapid chilling to prevent re-annealing ...

A Better Way to Isolate RNA AND Protein From the Same Sample

... A DNase I treatment is recommended for RNA that will be used for RT-PCR experiments, especially if using primers that do not flank introns, or for genes that have processed pseudogenes. Ambion's DNA-free DNase Treatment and Removal Reagents (available ...

PCR principles

... As is shown in the figure, the reaction uses two oligonucleotide primers that hybridize to opposite strands and flank the target DNA sequence that is to be amplified. The elongation of the primers is catalyzed by a heat-stable DNA polymerase (such ...

A New C-Terminal GST Vector for Protein Production in S. pombe

... Recombinant clones were identified by colony PCR analysis using vector-specific primers (the pESP-3 vector forward and reverse primers) that flank the MCS. A positive clone was chosen for further study. Figure 2 Panel A figures 2 Panel B ...
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