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Exonuclease in Biological Technology

Using catalysts to stamp nanopatterns without ink

... on the stamp with a molecular tether of amino acids. For this proof-of-principle demonstration, Toones team chose as a catalyst the biological enzyme exonuclease I, derived from the bacterium E. coli. In one set of experiments, the polyacrylamide stamp pattern bearing the tethered enzymes was then pressed ...

Pwo DNA Polymerase

... DNA polymerase and possesses an 3'5' exonuclease activity also known as proofreading activity. The enzyme has no detectable 5'3' exonuclease activity. Pwo DNA polymerase exhibits ... 5 min at this temperature. The inherent 3'5' exonuclease proofreading activity Pwo results in ...

HotStarTaq PCR Handbook

... 5'3' exonuclease activity: Yes ... 3'5' exonuclease activity: No ... exonuclease activity assay: Linearized ...

TripleMaster PCR System

... avoid primer/template degradation by 35 exonuclease activity and unspecific primer extension ... avoid primer/template degradation by 35 exonuclease activity and non-specific primer extension ... not recommended due to the inherent 35 exonuclease activity. 11.9 Primer ...

TripleMaster PCR System

... avoid primer/template degradation by 35 exonuclease activity and unspecific primer extension ... avoid primer/template degradation by 35 exonuclease activity and non-specific primer extension ... not recommended due to the inherent 35 exonuclease activity. 11.9 Primer ...

Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance

... is not introduced during packaging. Detecting exonuclease Contaminants The integrity of a restriction ... ends. Stratagene specifically assays for exonuclease activity by incubating an excess of restriction ... genomic E. coli DNA. Contaminating exonuclease is indicated if over 0.6% of the 3 ...

PCR Master

... It is a highly processive 5'3' DNA polymerase which lacks 3'5' exonuclease activity. Note: For additional convenience and reliable ... of amplification, because it is highly processive and free of exonuclease and nicking activities Increases sensitivity, because it ...

Taq DNA Polymerase

... It is a highly processive 5'3' DNA polymerase which lacks 3'5' exonuclease activity. Note: For additional convenience and ... of amplification, because it is highly processive and free of exonuclease and nicking activities Increases sensitivity, because it ...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

... 5 and 3 ends with 6-carboxyfluorescein (6-FAM) reporter and tetra methyl-6-carboxyrhodamine (TAMRA) quencher, respectively, is hydrolyzed by the 5 exonuclease activity of Taq DNA polymerase, causing an increase in fluorescent signal that is measured in real-time after each cycle of PCR amplification. ...

The iCycler iQ Detection System for TaqMan Assays, Rev B

... of the polymerase chain reaction (PCR*). One popular probe strategy is the TaqMan assay (PE Biosystems), which capitalizes on the 5' exonuclease activity of Taq polymerase to cleave a labeled hybridization probe during the extension phase of PCR (Holland et al. 1991). In ...

The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B

... 11, 10261030 (1993) Holland PM et al., Detection of specific polymerase chain reaction product by utilizing the 5'→3' exonuclease activity of Thermus aquaticus DNA polymerase, Proc Natl Acad Sci USA 88, 72767280 (1991) Rychlik W et al., Optimization of the ...

Recombinant HIV Reverse Transcriptase

... RT. Ambion's HIV RT is a high quality enzyme that has been tested for contaminating endonuclease and exonuclease activity. The enzyme is available in a convenient 500 U size. A 10X Reaction Buffer is supplied with the enzyme. ...

Fast, Accurate Assessment of siRNA-induced Gene Silencing

... and are labeled with a 5' fluorophore and 3' quencher. Quantification of PCR amplification relies on the 5' exonuclease activity of Taq polymerase to degrade the probe and separate the fluorophore from the quencher. ...

Pwo Master*: Simplify Setup of Your Proofreading PCRs

... Pwo DNA Polymerase. Pwo DNA Polymerase is a highly processive 5′3′ DNA polymerase that possesses 3′5′ exonuclease activity, also known as proofreading activity. This proofreading activity results in an approximate 18-fold increase in the fidelity of DNA ...

High Fidelity PCR Master

... Polymerase and Pwo DNA Polymerase[a thermostable enzyme with a proofreading (3'5' exonuclease) activity]. Due to the inherent 3'5' exonuclease proofreading activity of Tgo DNA Polymerase, the High Fidelity PCR Master increases the fidelity of DNA Synthesis approx. threefold ...

C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR

... from the thermophilic eubacterium Carboxydothermus hydrogenoformans, expressed in E. coli. The recombinant enzyme lacks the 5'3' exonuclease domain (RNase H activity) of the native enzyme. Application Produces first strand cDNA (for ...

C. therm. Polymerase One-Step RT-PCR System

... from the thermophilic eubacterium Carboxydothermus hydrogenoformans, expressed in E. coli. The recombinant enzyme lacks the 5'3' exonuclease domain (RNase H activity) of the native enzyme. The system also contains RT-PCR reaction buffer (5x conc.) with 12.5 mM MgCl 2 and ...

Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates

... experiments.2 In conclusion, the TripleMaster Enzyme Mix combines the efficiency of Taq DNA Polymerase with the 3' 5' exonuclease activity of a proofreading thermostable polymerase, along with a polymerase-enhancing factor that provides an extremely high ...

TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR

... depurination to a minimum. In addition, the TripleMaster Enzyme Mix combines the efficiency of Taq DNA Polymerase with the 3 5 exonuclease activity of a proofreading thermostable enzyme, along with a polymerase-enhancing factor that provides an extremely high ...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

... contains an adenine residue following the C terminus of the insertspecific sequence, which provides a stopping point for the exonuclease activity of Pfu DNA polymerase (figure 2). The N terminus of the insert DNA sequence may not contain an adenine residue for ...

Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase

... altering the enzymes high replication fidelity. This PCR enhancing factor was isolated from Pyrococcus and shown to lack both DNA polymerase and exonuclease activities (data not shown). PfuTurbo DNA polymerase is an optimized blend of cloned Pfu DNA polymerase and this PCR enhancing factor. PfuTurbo ...

The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature

... polymerase is formulated with a combination of antibodies that effectively neutralize DNA polymerase and 3'-5' (proofreading) exonuclease activities. Full enzyme activity is regained once the antibody is denatured during ramp-up to initial denaturation ....
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