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Eukaryotes in Biological Technology

International team of scientists discover clue to delay of life on Earth

... if bacteria cant fix nitrogen fast enough then eukaryotes a kind of organism that includes plants, pachyderms and people are in trouble because eukaryotes cannot fix nitrogen themselves at all. If ... would have had the upper hand, continues Anbar. eukaryotes depend on bacteria having an easy enough time ...

Biological electron transfer captured in real time

... level. Electron transfer is central to many chemical reactions in the cell. It has particular functional importance in cell respiration, which in eukaryotes takes place in the inner mitochondrial membrane, and in the cell membrane of prokaryotes. In cellular respiration molecules stemming from food are ...

Glossary for The BioPharm Guide to Fermentation and Cell Culture

... (genes) of a cell. Prokaryotes contain only a single chromosome; eukaryotes have more than one, made up of a complex of DNA, RNA, and ... organs, outer linings of kidneys, and so on). eukaryotes Complex organisms, often multicellular, whose cells contain ...

FERMENTATION BASICS

... capable of self duplication or of directing their own replication. Prokaryotes do not possess a true nucleus or a nuclear membrane, whereas eukaryotes have a nucleus enclosed within a distinct nuclear membrane. The non-cellular protists do not undergo self-replication, instead they direct their ...

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

... and Biochemistry, Yale University, 333 Cedar Street, New Haven, CT 06510. Introduction Genes of higher eukaryotes can be hundreds of kilobases in size. The need to rapidly clone and manipulate DNA molecules in that range has become acute in ...

RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs

... mesothelioma. Gene Redundancy In many cases, eliminating the expression of a single gene in higher eukaryotes can be tolerated even if that gene product functions in a critical pathway. This is because many critical ...

The QIAGEN Guide to Animal Cell Culture

... Welcome to a new series of articles aimed at providing useful hints for culturing animal cells (i.e., cells derived from higher eukaryotes such as mammals, birds, and insects ). This article introduces different types of animal cell cultures. The series will continue in ...

U937

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 620 V Time constant (T) ...

U-266

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 600 V Time constant (T) ...

t-HUVEC

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 670 V Time constant (T) ...

T98G

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 500 V Time constant (T) ...

Sp20-Ag14

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 160 V Time constant (T) ...

RAW 264.7

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 570 V Time constant (T) ...

Primary cells, rat heart muscle

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 100 V Time constant (T) ...

Neurospora crassa

... insert the cuvette into the device. Electroporation: Mode eukaryotes Voltage (V) 400 V Time constant (T) ...

NIH 3T3

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 480 V Time constant (T) ...

Neurospora crassa

... insert the cuvette into the device. Electroporation: Mode eukaryotes Voltage (V) 750 V Time constant (T) ...

Primary cells, rat heart endothel

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 400 V Time constant (T) ...

L2

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 180 V Time constant (T) ...

L929

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 400 V Time constant (T) ...

Jurkat

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 240 V Time constant (T) ...

HeLa

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 800 V Time constant (T) ...

HL-60

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 760 V Time constant (T) ...

H73C11

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 160 V Time constant (T) ...

Embryonic stem cells, mouse

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 300 V Time constant (T) ...

EL 4

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 480 V Time constant (T) ...

COS-7

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 600 V Time constant (T) ...

COS-1

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 600 V Time constant (T) ...

CHO

... be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 290 V Time constant (T) ...

BW 5147

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 530 V Time constant (T) ...

BALB/3T3

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 720 V Time constant (T) ...

B16

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 400 V Time constant (T) ...

A 20

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 600 V Time constant (T) ...

721.82

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 200 V Time constant (T) ...

721.221

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 200 V Time constant (T) ...

721.221

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 400 V Time constant (T) ...

293 T

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 300 V Time constant (T) ...

721.174

... must be free of air bubbles. Electroporation: Mode eukaryotes Voltage (V) 200 V Time constant (T) ...

Principles and applications of electrical cell fusion

... on the Multiporator. Developed in cooperation with Professor U. Zimmermann, this device is ideal for the electrofusion of eukaryotes and plant cells. Depending upon the chosen configuration, it is also possible to electroporate prokaryotes and yeasts, and/or ...
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