Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A
... in TBE or TAE buffer, with
or without ethidium
bromide, to fit your research requirements. In ... to cool for 10 min, and 14.5 l of 10 mg/ml ethidium
was added. The molten agarose ... TAE 3% ReadyAgarose gels
bromide) using a multichannel pipet. Several ...
Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
... but must be
supplemented with ethidium
bromide for use with FAM-Cy5, FAM-LC640, or
... pH 8, 50 mM KCl,
3 mM MgCl 2 ) with ethidium
bromide (see below) in place of the 1x well
... For FAM-Cy5 filter combination, add 100 l of ethidium
(10 mg/ml stock) to 0.9 ml of ...
Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis
... hours. PCR products were
detected by ethidium
Results ... cDNA detectable (Figure 1). Using ethidium
we were able to ... Figure 2 shows a
stained DGGE gel including 10 patient
Isolate It All: siRNA miRNA
Total RNA Native Protein
... acrylamide gel and stained
bromide. (B) Electropherogram of total RNA ... denaturing glyoxal agarose gel and stained with ethidium
(B) 1 g of each fraction ... acrylamide
gel and stained with ethidium
bromide. After electrotransfer, miR-16 miRNA
Two-step Gradient PCR*
Figure 1A. 1% Agarose gel stained with ethidium
Lane 1, 1 kb DNA ...
Figure 2A. 1% Agarose gel stained with ethidium
Lane 1, 1 kb DNA ...
Figure 3. 1% Agarose gel stained with ethidium
Lane 1, 1 kb DNA ...
Eppendorf Perfectprep Gel Cleanup Kit
1x TBE (Eppendorf)
Mastercycler gradient ... agarose gel containing 2 l
of 10 mg/ml ethidium
bromide. The gel was run in 1x TBE at 120 V for ... SeaKem LE agarose gel containing 2 ml of 10 mg/ml ethidium
gel was run in 1x TBE at ...
Eagle Eye Optical Filter Optimized for Imaging Silver-Stained
... for imaging nucleic acid gels
stained with ethidium
bromide. Because the system is also frequently ... Stratagenes
optical filter line now comprises ethidium
Green, 2 Coomassie Blue, ... compare the performances of the silver-stain and ethidium
filters. A stained gel was first ...
Improved Sensitivity for Staining RNA with SYBR Gold Stain
... with a nucleic acid stain. Although ethidium
bromide is commonly used
to stain RNA gels, we found that staining with ethidium
bromide often requires
as much as 1 g of RNA to ... up to 48 hours.
To compare SYBR Gold stain to ethidium
bromide staining, two identical gels
were run ...
Optimized Imaging of Protein Gels Stained with Coomassie
Brilliant Blue Dye
... a single filter specifically designed
bromide may be inadequate for use with other ... for stained
protein gels when compared to the ethidium
bromide-optimized filter. Sensitivity
was ... upon excitation with ultraviolet
light. This ethidium
bromide-optimized filter preferentially allows ...
Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
... UltraPure TM 10x TAE (Invitrogen, Cat. # 15558-026)
Bromide (Sigma, Cat. # E-1510)
◊ Tissue-Tek OCT ... agarose gel and separated by 120 V for 25 minutes. Gels are stained with ethidium
bromide and visualized using the Gel Doc 2000 TM Gel Documentation ....
Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae
Gels were stained in used buffer containing 0.5 g/ml ethidium
for 30 minutes and destained overnight.
Cell ... of chromosome copy number based
on densitometric scanning of an ethidium
bromide-stained gel or an X-ray
film of a probed blot is ...
The Bio-Rad Silver Stain, Rev E
Nucleic Acid Stain
More sensitive than ethidium
bromide 25x more sensitive
( 2 ) for ss DNA 22 1030x (<0.03 ... immunoelectrophoresis buffers
Other Related Kits from Bio-Rad
Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
... for cycling conditions). The
PCR products were analyzed on ethidium
bromide-stained agarose gels. The
primer pair that generated ... primers. The products of the
PCR reactions were analyzed on ethidium
bromide-stained agarose gels.
In contrast to their respective ...
UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
... of energy in the GS Gene Linker UV chamber
after staining with ethidium
bromide. The DNA was alkaline transferred
to Zeta-Probe GT ...
1. Following electrophoresis, stain the gel with 1.0 g/ml ethidium
(EtBr) for exactly 30 minutes with constant agitation. ...
Detection of Mutant K-ras in a Kindred With Hereditary Pancreatic Cancer by DGGE
... 110 bp
product was run on a 3% agarose gel and visualized using ethidium
Successful samples were run on a ... DGGE gel at 150 V for 2 hours at 56 C, then
stained with ethidium
bromide to find the optimal conditions
for parallel DGGE (Figure ...
Got Small RNA?
... resolved on a 15% denaturing acrylamide
gel and stained with ethidium
bromide or analyzed by solution hybridization
assay with the ... agarose
gel or a 15% denaturing acrylamide gel and stained with ethidium
The same fractions were also analyzed with an RNA 6000 ...
miRNA Expression in White Blood Cells
... analyzed on a 15% denaturing polyacrylamide
gel stained with ethidium
bromide (left panel). let-7 miRNA, and 5.8S
and 5S rRNA were ... blood cells was quantitated
by UV absorbance and analyzed by ethidium
bromide staining on a 15% denaturing
polyacrylamide gel. RNA ...
Using the TripleMaster PCR System for Robust Amplification of
GC-Rich DNA Templates
... Molecular Applications)
1x TBE (Eppendorf)
Alpha Imager CCD Camera Documentation System ... on a 2.0% agarose gel, run at 6 V/cm, stained
in a 0.5 mg/ml ethidium
bromide solution and photographed with a CCD camera.
TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
... OmniPur Agarose (EM Science)
0.5x TBE (Eppendorf)
The performance of the TripleMaster PCR System ... on a 1.0% agarose gel, run for 16 hours,
stained in a 0.5 mg/ml ethidium
bromide solution and photographed.
Table 1: Primers sequences ...
Simple and Rapid Isolation of Plant RNA
Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
... were run on a
1% agarose formaldehyde gel and stained with ethidium
1: 0.24-9.5 kb RNA Ladder from ...
Figure 2: ethidium
bromide-stained 1% agarose
gel. The gel demonstrates the ...
Recovery of DNA from LMP Agarose1
... TBE gels are much more difficult to solubilize.
Stain gel with ethidium
Bromide, visualize with a longwave UV light,
and carefully ... with a sharp razor blade.
Caution: Wear gloves when handling ethidium
Bromide and stained gels.
Transfer slice to a ...
Restriction Digests of DNA Isolated with the Perfectprep
Gel Cleanup Kit
... 1x TBE (Eppendorf)
Bromide 10 mg/ml (Sigma)
agarose gel containing 2 l of 10 mg/ml ethidium
bromide. 18 lanes
were loaded. The gel ... hours. The gel was stained in 50 ng/l
bromide solution for 20 minutes and destained in ...
Oil-Free PCR Using the Hot Top Assembly
... were electrophoresed through the same
gel, stained with ethidium
bromide and visualized using the Eagle Eye
II still video ... were electrophoresed
through the same gel, stained with ethidium
bromide and visualized using
the Eagle Eye II still video ...
Evaluation of RNA isolated from human tissues: quality control for surgical and postmortem samples
... it is important to test the RNA integrity. This has been traditionally performed by agarose gel electrophoresis and staining the RNA either with ethidium
bromide or SYBR Green dye. In the past, the ratio between the ribosomal bands (28S:18S) was viewed as the primary indicator of RNA integrity, with a ...
Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000
... Human BAC library plate #707 using the Eppendorf Perfectprep 96 kit. A volume of 10 μL from 24 samples were run on a 1% agarose gel containing ethidium
bromide (Figure 2).
Figure 2: BAC DNA isolated with the Eppendorf BAC 96 kit on the CaliperLS Sciclone ALH 3000. This shows 24 of the ...
LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
... the performance of the LIVE/DEAD Viability/Cytoxicity Assay Kit for animal cells. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium
homodimer (EthD-1), to stain live and dead cells simultaneously. Cal AM is an electrically neutral, nonfluorescent, esterase substrate that diffuses ...
Simple Approaches for Optimization of RT-PCR
... transfer tubes to ice.
7. Analysis Analyze sample (typically 1 to 10 μl aliquots) by agarose gel electrophoresis with staining by ethidium
bromide. Visualize PCR product in gel with a UV transilluminator or fluorescence imager.
Results and Discussio n
Oragene and Whole Genome Amplification with GenomiPhi
The amplified DNA was quantified using the F/D Protocol. The molecular
weight was analyzed by agarose gel electrophoresis with ethidium
staining and comparison with a Lambda-Hind III digest ladder.
Polymerase Chain Reaction
WGA and ...
DNA Stability with Oragene
... and processed by the standard Oragene protocol. 200 ng of
DNA from each sample was analyzed by agarose gel electrophoresis with
bromide staining. The molecular weight of the extracted DNA was
determined by comparison with a Lambda-Hind III digest ladder.
Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes
... 89 mM boric acid, Mallinckrodt Inc., St. Louis,
MO; 2 mM ethylenediamine tetraacetic acid, Fisher Scientific, Pittsburgh,
bromide (Bio-Rad Laboratories, Hercules, CA) was added to
the agarose solution at a concentration of 0.5 mg/ml before the gel was
Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
... C and a temperature ramp of 1 C/hr.
After electrophoresis, gels were equilibrated in 1.25x TAE buffer for
15 min., stained in ethidium
bromide (25 mg/ml in TAE) for 20 min. and
washed in TAE before viewing and photographing under UV light (302 nm).
Heteroduplex Analysis Using the DCode System
... electrophoresed on the DCode system at 100 V for 16 hours at room temperature.
After electrophoresis, the gel was stained in 50 g/ml ethidium
in 0.6x TBE buffer for 5 minutes and destained in buffer for 10 minutes.
The gel was imaged under UV transillumination. ...
Detection of Mutations in the CYP21 Gene Using the DCode System
... formamide. The gels were run in 1x TAE buffer, at 80 V overnight. After
electrophoresis, the gels were stained in TAE buffer containing ethidium
bromide and subsequently the resolved bands were visualized by ultraviolet
PFGE Analysis of Chromosomal DNA From a Single Hair Root
... 14 C. Electrophoresis was conducted at 200 volts for 24 hours with
a 50-90 second switch time ramp.
Gels were first stained with ethidium
bromide (Figure 1A). Southern blotting
and hybridization were performed as previously described. 2,3 The autoradiograph
of the ...
Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light
... (Figure 2, lane 1). With increased levels of
UV irradiation, the amount of detectable PCR product decreases, as visualized
bromide staining. After 30 minutes of UV irradiation, both
of the templates have been inactivated. As demonstrated in Figure 1 and
Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
... PCR products were loaded onto 6%
acrylamide gels and run at constant temperature (60 C) for 45 h, followed
by staining in ethidium
bromide and visualization with a standard UV transilluminator.
For the tautog, no previous genetic data ...
Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities
... probe. Serial dilutions
of pHSVlac were used to generate a standard curve. Diluted plasmid was
quantified by photoimaging in an ethidium
bromide-stained 0.8% agarose
Results and Discussion
EFFECT OF PURIFICATION METHOD ON PLASMID ...
Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
... vs. 3 hr for
The purified PCR product is immediately available for sequencing PCR
No exposure to ethidium
bromide or UV light
It is possible to obtain sequencing results for a PCR product within
Only one specific PCR ...
Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations
... detection system (Bio-Rad) as described
in the figure legends. After electrophoresis, the gels were stained in
0.5 g/ml ethidium
bromide in TAE running buffer for 5 minutes, destained
for 20 minutes, and then photographed on a UV transilluminator.