Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A

... in TBE or TAE buffer, with or without ethidium bromide, to fit your research requirements. In ... to cool for 10 min, and 14.5 l of 10 mg/ml ethidium bromide was added. The molten agarose ... TAE 3% ReadyAgarose gels (with ethidium bromide) using a multichannel pipet. Several ...

Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A

... but must be supplemented with ethidium bromide for use with FAM-Cy5, FAM-LC640, or ... pH 8, 50 mM KCl, 3 mM MgCl 2 ) with ethidium bromide (see below) in place of the 1x well ... For FAM-Cy5 filter combination, add 100 l of ethidium bromide (10 mg/ml stock) to 0.9 ml of ...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

... hours. PCR products were detected by ethidium bromide staining. Results ... cDNA detectable (Figure 1). Using ethidium bromide staining, we were able to ... Figure 2 shows a representative ethidium stained DGGE gel including 10 patient ...

Isolate It All: siRNA miRNA Total RNA Native Protein

... acrylamide gel and stained with ethidium bromide. (B) Electropherogram of total RNA ... denaturing glyoxal agarose gel and stained with ethidium bromide. (B) 1 g of each fraction ... acrylamide gel and stained with ethidium bromide. After electrotransfer, miR-16 miRNA ...

Two-step Gradient PCR*

... Figure 1A. 1% Agarose gel stained with ethidium bromide. Lane 1, 1 kb DNA ... Figure 2A. 1% Agarose gel stained with ethidium bromide. Lane 1, 1 kb DNA ... Figure 3. 1% Agarose gel stained with ethidium bromide. Lane 1, 1 kb DNA ...

Eppendorf Perfectprep Gel Cleanup Kit

... 1x TBE (Eppendorf) ethidium Bromide (Sigma) Mastercycler gradient ... agarose gel containing 2 l of 10 mg/ml ethidium bromide. The gel was run in 1x TBE at 120 V for ... SeaKem LE agarose gel containing 2 ml of 10 mg/ml ethidium bromide. The gel was run in 1x TBE at ...

Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels

... for imaging nucleic acid gels stained with ethidium bromide. Because the system is also frequently ... Stratagenes optical filter line now comprises ethidium bromide, SYBR Green, 2 Coomassie Blue, ... compare the performances of the silver-stain and ethidium bromide filters. A stained gel was first ...

Improved Sensitivity for Staining RNA with SYBR Gold Stain

... with a nucleic acid stain. Although ethidium bromide is commonly used to stain RNA gels, we found that staining with ethidium bromide often requires as much as 1 g of RNA to ... up to 48 hours. To compare SYBR Gold stain to ethidium bromide staining, two identical gels were run ...

Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye

... a single filter specifically designed for ethidium bromide may be inadequate for use with other ... for stained protein gels when compared to the ethidium bromide-optimized filter. Sensitivity was ... upon excitation with ultraviolet light. This ethidium bromide-optimized filter preferentially allows ...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

... UltraPure TM 10x TAE (Invitrogen, Cat. # 15558-026) ◊ ethidium Bromide (Sigma, Cat. # E-1510) ◊ Tissue-Tek OCT ... agarose gel and separated by 120 V for 25 minutes. Gels are stained with ethidium bromide and visualized using the Gel Doc 2000 TM Gel Documentation ....

Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae

... Gels were stained in used buffer containing 0.5 g/ml ethidium bromide for 30 minutes and destained overnight. Cell ... of chromosome copy number based on densitometric scanning of an ethidium bromide-stained gel or an X-ray film of a probed blot is ...

The Bio-Rad Silver Stain, Rev E

... Nucleic Acid Stain More sensitive than ethidium bromide 25x more sensitive ( 2 ) for ss DNA 22 1030x (<0.03 ... immunoelectrophoresis buffers DNA standards ethidium bromide Other Related Kits from Bio-Rad Protein ...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

... for cycling conditions). The PCR products were analyzed on ethidium bromide-stained agarose gels. The primer pair that generated ... primers. The products of the PCR reactions were analyzed on ethidium bromide-stained agarose gels. In contrast to their respective ...

UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs

... of energy in the GS Gene Linker UV chamber after staining with ethidium bromide. The DNA was alkaline transferred to Zeta-Probe GT ... 1. Following electrophoresis, stain the gel with 1.0 g/ml ethidium bromide (EtBr) for exactly 30 minutes with constant agitation. ...

Detection of Mutant K-ras in a Kindred With Hereditary Pancreatic Cancer by DGGE

... 110 bp product was run on a 3% agarose gel and visualized using ethidium bromide staining. Successful samples were run on a ... DGGE gel at 150 V for 2 hours at 56 C, then stained with ethidium bromide to find the optimal conditions for parallel DGGE (Figure ...

Got Small RNA?

... resolved on a 15% denaturing acrylamide gel and stained with ethidium bromide or analyzed by solution hybridization assay with the ... agarose gel or a 15% denaturing acrylamide gel and stained with ethidium bromide. The same fractions were also analyzed with an RNA 6000 ...

miRNA Expression in White Blood Cells

... analyzed on a 15% denaturing polyacrylamide gel stained with ethidium bromide (left panel). let-7 miRNA, and 5.8S and 5S rRNA were ... blood cells was quantitated by UV absorbance and analyzed by ethidium bromide staining on a 15% denaturing polyacrylamide gel. RNA ...

Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates

... Molecular Applications) 1x TBE (Eppendorf) ethidium Bromide (Sigma) Alpha Imager CCD Camera Documentation System ... on a 2.0% agarose gel, run at 6 V/cm, stained in a 0.5 mg/ml ethidium bromide solution and photographed with a CCD camera. In ...

TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR

... OmniPur Agarose (EM Science) 0.5x TBE (Eppendorf) ethidium Bromide (Sigma) The performance of the TripleMaster PCR System ... on a 1.0% agarose gel, run for 16 hours, stained in a 0.5 mg/ml ethidium bromide solution and photographed. Table 1: Primers sequences ...

Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit

... were run on a 1% agarose formaldehyde gel and stained with ethidium bromide. Lane 1: 0.24-9.5 kb RNA Ladder from ... Figure 2: ethidium bromide-stained 1% agarose gel. The gel demonstrates the ...

Recovery of DNA from LMP Agarose1

... TBE gels are much more difficult to solubilize. Stain gel with ethidium Bromide, visualize with a longwave UV light, and carefully ... with a sharp razor blade. Caution: Wear gloves when handling ethidium Bromide and stained gels. Transfer slice to a ...

Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit

... 1x TBE (Eppendorf) ethidium Bromide 10 mg/ml (Sigma) Mastercycler ... agarose gel containing 2 l of 10 mg/ml ethidium bromide. 18 lanes were loaded. The gel ... hours. The gel was stained in 50 ng/l ethidium bromide solution for 20 minutes and destained in ...

Oil-Free PCR Using the Hot Top Assembly

... were electrophoresed through the same gel, stained with ethidium bromide and visualized using the Eagle Eye II still video ... were electrophoresed through the same gel, stained with ethidium bromide and visualized using the Eagle Eye II still video ...

Evaluation of RNA isolated from human tissues: quality control for surgical and postmortem samples

... it is important to test the RNA integrity. This has been traditionally performed by agarose gel electrophoresis and staining the RNA either with ethidium bromide or SYBR Green dye. In the past, the ratio between the ribosomal bands (28S:18S) was viewed as the primary indicator of RNA integrity, with a ...

Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000

... Human BAC library plate #707 using the Eppendorf Perfectprep 96 kit. A volume of 10 μL from 24 samples were run on a 1% agarose gel containing ethidium bromide (Figure 2). Figure 2: BAC DNA isolated with the Eppendorf BAC 96 kit on the CaliperLS Sciclone ALH 3000. This shows 24 of the ...

LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)

... the performance of the LIVE/DEAD Viability/Cytoxicity Assay Kit for animal cells. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium homodimer (EthD-1), to stain live and dead cells simultaneously. Cal AM is an electrically neutral, nonfluorescent, esterase substrate that diffuses ...

Simple Approaches for Optimization of RT-PCR

... transfer tubes to ice. 7. Analysis Analyze sample (typically 1 to 10 μl aliquots) by agarose gel electrophoresis with staining by ethidium bromide. Visualize PCR product in gel with a UV transilluminator or fluorescence imager. Results and Discussio n ...

Oragene and Whole Genome Amplification with GenomiPhi

... The amplified DNA was quantified using the F/D Protocol. The molecular weight was analyzed by agarose gel electrophoresis with ethidium bromide staining and comparison with a Lambda-Hind III digest ladder. Polymerase Chain Reaction WGA and ...

DNA Stability with Oragene

... and processed by the standard Oragene protocol. 200 ng of DNA from each sample was analyzed by agarose gel electrophoresis with ethidium bromide staining. The molecular weight of the extracted DNA was determined by comparison with a Lambda-Hind III digest ladder. ...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

... 89 mM boric acid, Mallinckrodt Inc., St. Louis, MO; 2 mM ethylenediamine tetraacetic acid, Fisher Scientific, Pittsburgh, PA). ethidium bromide (Bio-Rad Laboratories, Hercules, CA) was added to the agarose solution at a concentration of 0.5 mg/ml before the gel was ...

Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis

... C and a temperature ramp of 1 C/hr. After electrophoresis, gels were equilibrated in 1.25x TAE buffer for 15 min., stained in ethidium bromide (25 mg/ml in TAE) for 20 min. and washed in TAE before viewing and photographing under UV light (302 nm). Various ...

Heteroduplex Analysis Using the DCode System

... electrophoresed on the DCode system at 100 V for 16 hours at room temperature. After electrophoresis, the gel was stained in 50 g/ml ethidium bromide in 0.6x TBE buffer for 5 minutes and destained in buffer for 10 minutes. The gel was imaged under UV transillumination. ...

Detection of Mutations in the CYP21 Gene Using the DCode System

... formamide. The gels were run in 1x TAE buffer, at 80 V overnight. After electrophoresis, the gels were stained in TAE buffer containing ethidium bromide and subsequently the resolved bands were visualized by ultraviolet (UV) transillumination. ...

PFGE Analysis of Chromosomal DNA From a Single Hair Root

... 14 C. Electrophoresis was conducted at 200 volts for 24 hours with a 50-90 second switch time ramp. Gels were first stained with ethidium bromide (Figure 1A). Southern blotting and hybridization were performed as previously described. 2,3 The autoradiograph of the ...

Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light

... (Figure 2, lane 1). With increased levels of UV irradiation, the amount of detectable PCR product decreases, as visualized by ethidium bromide staining. After 30 minutes of UV irradiation, both of the templates have been inactivated. As demonstrated in Figure 1 and ...

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

... PCR products were loaded onto 6% acrylamide gels and run at constant temperature (60 C) for 45 h, followed by staining in ethidium bromide and visualization with a standard UV transilluminator. Results For the tautog, no previous genetic data ...

Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities

... probe. Serial dilutions of pHSVlac were used to generate a standard curve. Diluted plasmid was quantified by photoimaging in an ethidium bromide-stained 0.8% agarose gel. Results and Discussion EFFECT OF PURIFICATION METHOD ON PLASMID ...

Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A

... vs. 3 hr for gel purification) The purified PCR product is immediately available for sequencing PCR No exposure to ethidium bromide or UV light It is possible to obtain sequencing results for a PCR product within 1 day Only one specific PCR ...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

... detection system (Bio-Rad) as described in the figure legends. After electrophoresis, the gels were stained in 0.5 g/ml ethidium bromide in TAE running buffer for 5 minutes, destained for 20 minutes, and then photographed on a UV transilluminator. ...