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Equilibrium in Biological Technology

A supercharged metal-ion generator

... more "excess" plasma the system runs away, until it finally reaches a new equilibrium at a much higher peak-power level than before. Achieving equilibrium "Three quantities determine the self-sputtering threshold," Anders ...

DOE awards $377 million in funding for 46 energy frontier research centers

... University (Evanston, IL) $19 million for five years to synthesize, characterize, and understand new classes of materials under conditions far from equilibrium relevant to solar energy conversion, storage of electricity and hydrogen, and catalysis. University of Notre Dame (Notre Dame, IN) $18.5 million ...

DNA Software, Inc. Awarded $2.5 M in NIH Funding to Develop Pioneering Nucleic Acid-based Technologies

... PCR Reaction Rates and Diagnostics with Modified Nucleotides While current software programs can model the thermodynamics (i.e. the results at equilibrium after a long time) of DNA and RNA reactions, none can model the kinetics (i.e. how fast the reaction takes place). In addition, most software can ...

Solar cells, geological storage research receive DOE funding at the University of Texas at Austin

... far beyond the current focus on the storage of greenhouse gases to understanding the stability of geological systems and materials that are far from equilibrium for long periods of time--a Grand Challenge problem." The interdisciplinary team in the Cockrell School of Engineering and the Jackson School of ...

Incorrectly cleaved protein leads to schizophrenia

... has also shown that there is a disturbance of the activity of the frontal and temporal areas in the brain, which is connected with a disturbed equilibrium among the substances (proteins) that are needed for neuronal functioning. Schizophrenia is treated using anti-psychotic and neuroleptic medicines. ...

Rutgers physicist earns prestigious Packard Foundation Science and Engineering Fellowship

... biggest problems in physics it took scientists many decades to solve, he noted. But their description was for situations where the superfluid is in equilibrium or in some way close to it; that is, where the unusual states of matter that we see near absolute zero are constant in time. Yuzbashyans latest ...

Madison-based Mithridion seeks to capitalize on Alzheimer's breakthrough

... of all cases. As for the other 90 percent, Alzheimer's contracted sporadically and for no apparent reason, the condition "probably is due to an equilibrium balance shift between the good- and the bad-cut protein," Johnson said. "So as you age, the good-cut protein goes down and the bad-cut protein ...

A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity

... agent (produced by Bristol-Meyers Oncology) functions by stabilization of microtubules so they cannot depolymerize. It effectively shifts the equilibrium in cells towards microtubule assembly, disrupting the normal operation of the microtubule network and thereby arresting the mitotic process. ...

Receptor Binding Assays using Fluorescence Polarization Detection: the [FP]2TM Characterized Tracers

... assays of G protein-coupled receptors. J. Biomol. Screen. 5:329-334. 10. Prystay, L., Gosselin, M., Banks, P. (2001). Determination of equilibrium dissociation constants in fluorescence polarization. J. Biomol. Screen. 6:141-150. 11. Owicki J.C. (2000) Fluorescence polarization and ...

Photoluminescence Spectroscopy of Quantum Dots, monitored by the Spex FluoroMax

... of these ions; and (c) the binary structures of these ions have various lattice constants. Relaxation of the ionic bonds to equilibrium positions may lead to local order in the structure and a larger than expected reduction in the band-gap. ...

Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor

... of TGF-1 binding to hTRIIs-Fc (Figure 2). The kinetics of 125 I-labeled TGF-1 binding, performed at room temperature, were relatively rapid, since equilibrium binding was usually achieved after just two to three hours of incubation. Meaningful values for binding ...

Analysis of Fuel Oxygenates by U.S. EPA Method 8260B Using Headspace Trap with GC/MS

... U.S. Environmental Protection Agency (EPA) Method 8260B. The headspace trap uses heat to extract (partition) the compounds out of the water into an equilibrium headspace, instead of purging. This transports a significant and highly reproducible fraction of the analytes from the sample onto a thermal ...

Practical Applications of HyperDSC in a Pharmaceutical Laboratory

... setting, even at a rate as high as 500 C/min. Thus, for an experiment beginning at -65 C and carried out at 200 C/min, the apparatus reaches equilibrium at about 30 C, the temperature from which any transition can be measured. By comparison, a classical heat-flux DSC can take 30 to 60 seconds and is ...

USB OptiKinase Eliminates Base Bias in Labeling of Oligonucleotide 5'-Ends

... these is that OptiKinase overcomes base bias, allowing uniform labeling of oligonucleotides (Fig. 2). Additionally, OptiKinase shifts the reaction equilibrium toward phosphorylation, allowing efficient labeling with relatively lower amounts of radiolabel (Fig. 3). It performs well across a range of enzyme ...

The development of a serotonin 5-HT2C receptor-binding assay using the LEADseeker Multimodality Imaging System

... protocol and DMSO included at an assay concentration of 2% (v/v). Readings were taken over an increased incubation time of 18 h. The assay reached equilibrium after approximately 5 h and appeared to be stable for at least a further 13 h (Fig 4).

Z analysis ...

The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System

... out using reagent concentrations as described in the protocol. Measurements were made over an assay incubation time of 20.5 h. The assay reached equilibrium after 6-h incubation at room temperature, and the signal was stable for at least another 14 h (Fig 4).

...

The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System

... out using reagent concentrations as described in the protocol. Measurements were made over an assay incubation time of 18 h. The assay reached equilibrium after 4 h incubation at room temperature, and the signal was stable for at least 14 h (Fig 4).

...

The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System

... out using reagent concentrations as described in the protocol. Measurements were made over an assay incubation time of 20 h. The assay reached equilibrium after 5-h incubation at room temperature and the signal was stable for at least another 5 h (Fig 4).

...

The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System

... out using reagent concentrations as described in the protocol. Measurements were made over an assay incubation time of 20.5 h. The assay reached equilibrium after 8-h incubation at room temperature and the signal was stable for at least another 12.5 h (Fig 4).

...

The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System

... protocol and DMSO included at an assay concentration of 2.5% (v/v). Readings were taken over an increased incubation time of 18 h. The assay reached equilibrium after approximately 7 h and was stable for at least a further 5 h as demonstrated by the results shown in Figure 4.

Z ...

Measuring RNase Activity A Real-time Kinetic Analysis

... the rate of increase in fluorescence was constant for several minutes. Under these conditions, the reaction is at equilibrium and the rate of increase in fluorescence (the slope of the line) can be used to calculate the initial rate (or ...
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