Frost & Sullivan Awards Precision Biosciences for Technology Innovation and Molecular Design Process in the Genomics Market
... in order to identify best practices in the industry.
About Precision Biosciences
Precision's mission is to utilize its engineered endonuclease
technology to become the world leader in the field of genomic molecular biology. Precision's proprietary Directed Nuclease Editor(TM) (DNE) ...
Polycystic Kidney Disease Assay at Cornell University Uses Transgenomic's Surveyor Nuclease & WAVE Technology
... that we have invested in these technologies."
Transgenomic's SURVEYOR Nuclease is a proprietary mismatch-specific endonuclease
that efficiently detects any mismatches in double-stranded DNA and cleaves at the site of DNA mutations. It identifies all base substitutions, ...
Alfacell's ONCONASE(R) and R-Amphinase Show Anti-Tumor Activity in Chronic Lymphocytic Leukemia and Acute Myeloblastic Leukemia Cells
... effect of ONCONASE and R-Amph was evident after 48 and 72 hours of treatment, respectively. Synergistic activity was seen in the combination of the endonuclease
plus either purine analogue. The combination of doxorubicin with ONCONASE or R-Amph demonstrated an increase in pro-apoptotic activity when compared ...
Transgenomic Awarded SBIR Grant to Support Development of SURVEYOR(R) Endonuclease Adaptor-ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation
... genome analysis for pharmacogenomic studies in personalized medicine and
bacterial drug resistance research."
Adaptor-ligated Libraries (SEAL) is a cost-effective
and high throughput enabling technology for whole genome analysis under
development by ...
Transgenomic Introduces SURVEYOR(R) Endonuclease Adaptor-Ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation
... identify point mutations and
small insertion/deletions at 100 to 1000 times lower sequencing intensity
than current methods."
Adaptor-ligated Libraries (SEAL) is a
cost-effective and high throughput enabling technology for whole genome
analysis under development by ...
Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal
... sample from each packaging of a
is confirmed prior to shipment to demonstrate ... preparation is free from contaminating endonuclease
activities, the overdigested ... activity by incubating an excess
of restriction endonuclease
for 4 hours with a mixture of single- ...
HotStarTaq PCR Handbook
... indicated under Exo.
Plasmid DNA is incubated with HotStarTaq DNA Polymerase
in PCR Buffer. endonuclease
activity per unit
of enzyme is indicated under ...
GenomiPhi amplification of FFPE tissue DNA for array CGH
... PicoGreen™ dsDNA Assay Kit (Invitrogen)
HyperLadder I DNA ladder (BioLine)
Dpn II restriction endonuclease
(New England BioLabs)
DNA extraction from FFPE tissue slides
Genomic DNA was isolated ...
Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System
... using a temperature
cycler (Ericomp Inc., San Diego, CA). Both oligonucleotide primers had
GC clamps. 2 Following restriction endonuclease
digestion, the samples
were subjected to DGGE.
The amplification reactions were performed in 50 mM Tris-HCl, pH 9 (at
Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
... Zimmerly, S., Guo, H., Eskes, R., Yang, J., Perlman, P. S. and Lambowitz,
A. M., A group II intron RNA is a catalytic component of a DNA endonuclease
involved in intron mobility, Cell, 83 , 529-538 (1995).
Biolistic is a registered trademark of E.I.DuPont deNemours ...
Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach
... 1 (40 mM Tris, pH 8.0). Bacteria were lysed by
rapid freezethawing (10 cycles), and were vortexed between cycles for
12 min. endonuclease
was added to a concentration of 150 U/ml and samples
were incubated at room temperature for 30 min to digest DNA. Samples were
Recombinant HIV Reverse Transcriptase
Ambion's HIV RT is a high quality enzyme that has been
tested for contaminating endonuclease
and exonuclease activity. The enzyme
is available in a convenient 500 U size. A 10X Reaction Buffer is supplied
with the ....
Measuring RNase Activity A Real-time Kinetic Analysis
... for most
commercially produced RNase A (and its glycosylated derivative,
RNase A is an endonuclease
that cleaves 3' of C
and U residues. It is commonly used in molecular biology
applications such as the removal of ...
Constructing Directional cDNA Libraries from Limited Amounts of RNA
... to covalently link the vector and insert; consequently, the
background of self-ligated vector is reduced. Since PCR products are cloned
reactions, cleavage of inserts at internal restriction
sites is avoided.
On another exciting front, the kit can be used to make cDNA libraries ...