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Endonuclease in Biological Technology

Frost & Sullivan Awards Precision Biosciences for Technology Innovation and Molecular Design Process in the Genomics Market

... in order to identify best practices in the industry. About Precision Biosciences Precision's mission is to utilize its engineered endonuclease technology to become the world leader in the field of genomic molecular biology. Precision's proprietary Directed Nuclease Editor(TM) (DNE) ...

Polycystic Kidney Disease Assay at Cornell University Uses Transgenomic's Surveyor Nuclease & WAVE Technology

... that we have invested in these technologies." Technical Information Transgenomic's SURVEYOR Nuclease is a proprietary mismatch-specific endonuclease that efficiently detects any mismatches in double-stranded DNA and cleaves at the site of DNA mutations. It identifies all base substitutions, ...

Alfacell's ONCONASE(R) and R-Amphinase Show Anti-Tumor Activity in Chronic Lymphocytic Leukemia and Acute Myeloblastic Leukemia Cells

... effect of ONCONASE and R-Amph was evident after 48 and 72 hours of treatment, respectively. Synergistic activity was seen in the combination of the endonuclease plus either purine analogue. The combination of doxorubicin with ONCONASE or R-Amph demonstrated an increase in pro-apoptotic activity when compared ...

Transgenomic Awarded SBIR Grant to Support Development of SURVEYOR(R) Endonuclease Adaptor-ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation

... genome analysis for pharmacogenomic studies in personalized medicine and bacterial drug resistance research." About SEAL SURVEYOR endonuclease Adaptor-ligated Libraries (SEAL) is a cost-effective and high throughput enabling technology for whole genome analysis under development by ...

Transgenomic Introduces SURVEYOR(R) Endonuclease Adaptor-Ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation

... identify point mutations and small insertion/deletions at 100 to 1000 times lower sequencing intensity than current methods." About SEAL SURVEYOR endonuclease Adaptor-ligated Libraries (SEAL) is a cost-effective and high throughput enabling technology for whole genome analysis under development by ...

Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance

... sample from each packaging of a restriction endonuclease is confirmed prior to shipment to demonstrate ... preparation is free from contaminating endonuclease and exonuclease activities, the overdigested ... activity by incubating an excess of restriction endonuclease for 4 hours with a mixture of single- ...

HotStarTaq PCR Handbook

... indicated under Exo. endonuclease activity assay: Plasmid DNA is incubated with HotStarTaq DNA Polymerase in PCR Buffer. endonuclease activity per unit of enzyme is indicated under ...

GenomiPhi amplification of FFPE tissue DNA for array CGH

... PicoGreen™ dsDNA Assay Kit (Invitrogen) HyperLadder I DNA ladder (BioLine) Dpn II restriction endonuclease (New England BioLabs)

Methods DNA extraction from FFPE tissue slides Genomic DNA was isolated ...

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

... using a temperature cycler (Ericomp Inc., San Diego, CA). Both oligonucleotide primers had GC clamps. 2 Following restriction endonuclease digestion, the samples were subjected to DGGE. The amplification reactions were performed in 50 mM Tris-HCl, pH 9 (at ...

Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

... Zimmerly, S., Guo, H., Eskes, R., Yang, J., Perlman, P. S. and Lambowitz, A. M., A group II intron RNA is a catalytic component of a DNA endonuclease involved in intron mobility, Cell, 83 , 529-538 (1995). Biolistic is a registered trademark of E.I.DuPont deNemours ...

Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

... 1 (40 mM Tris, pH 8.0). Bacteria were lysed by rapid freezethawing (10 cycles), and were vortexed between cycles for 12 min. endonuclease was added to a concentration of 150 U/ml and samples were incubated at room temperature for 30 min to digest DNA. Samples were ...

Recombinant HIV Reverse Transcriptase

... RT. Ambion's HIV RT is a high quality enzyme that has been tested for contaminating endonuclease and exonuclease activity. The enzyme is available in a convenient 500 U size. A 10X Reaction Buffer is supplied with the ....

Measuring RNase Activity A Real-time Kinetic Analysis

... for most commercially produced RNase A (and its glycosylated derivative, RNase B). RNase A is an endonuclease that cleaves 3' of C and U residues. It is commonly used in molecular biology applications such as the removal of ...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

... to covalently link the vector and insert; consequently, the background of self-ligated vector is reduced. Since PCR products are cloned without endonuclease reactions, cleavage of inserts at internal restriction sites is avoided. On another exciting front, the kit can be used to make cDNA libraries ...
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