Scientists fix bugs in our understanding of evolution
... however, to judge if a length difference between two sequences is a deletion
in one or an insertion in the other sequence. For correct alignment of ... lab. "Say we are comparing the DNA of human and chimp and can't tell if a deletion
or an insertion happened. To solve this our tool automatically invokes ...
New Study Raises Questions About Prostate Cancer Therapies Targeting Insulin-Like Growth Factor Receptor
... compromised. The results were as predicted: Prostate
of IGF-1R facilitated the emergence of
aggressive prostate cancer in the ... mechanism,
according to the authors. Title of the paper is "Conditional deletion
insulin-like growth factor-1 receptor in prostate epithelium."
Use it or lose it? Researchers investigate the dispensability of our DNA
... forces at work. "With this in mind, we set out to examine the genomic data, much as someone would examine archaeological data, in search of similar deletion
events that have happened naturally, and more importantly, were retained in the wild."
"An analogy I like to entertain is that of plate tectonics: ...
Ortho Biotech Oncology Research & Development Unites Johnson & Johnson Biopharmaceutical Oncology R&D Assets
Human Double Minute 2 (Hdm2)
The Hdm2 oncogene is activated in cancers through various mechanisms,
including gene amplification and deletion
of upstream tumor suppressors.
Hdm2 over-expression induces tumor formation, and Hdm2 levels correlate
with sporadic tumor incidence in humans(i). ...
NimbleGen microarrays speed genetic findings
One form of retardation, caused by a large deletion
that spans six genes on chromosome 17, has ... were able to pinpoint the region of the specific deletion
using high resolution CGH microarrays.
In a ... added.
Although the deletion
on chromosome 17 potentially accounts for only ...
Heteroduplex Analysis Using the DCode System
... base insertion (+TC) and ΔF311, three base deletion
(-TTC). The mutations from exon 10 were a heterozygous ΔF508, three base
(-CTT), a heterozygous compound sample Q493 (C to ... a heterozygous ΔI507, three base
(-ATC). Samples were provided by Dr. L. ...
Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
lyase (strain CC-48) or a non-photosynthetic, acetate-requiring deletion
mutation in the atpB gene (strain CC-373) were used for ...
mutant (S. cerevisiae strain 161) containing a deletion
intron 2 of the COXI gene (Zimmerly, et al., 1995) and ...
Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon
Allelic Discrimination Kits
A), and MTHFR (C677T) genes, as well as of a 32-base-pair deletion
in the human
CCR5 (D32) gene. Each kit includes two
allele-specific ... for T-tropic HIV-1
that results in a truncated protein 5
Third Wave Releases Gene Test
... the 2D6 gene that affect individual drug response, including single nucleotide polymorphisms, single and multiple base-pair deletions and whole gene deletion
and duplication. While 2D6 genotyping is complex, the performance characteristics of the Invader technology, most notably its specificity, reduce or ...
SNPs Powerful Tools for Association Studies
... recessive, or sex-linked patterns.
Mutation A change in the DNA sequence. A mutation can be a change from one base to another, a deletion
of bases, or an addition of bases. Typically, the term mutation is used to refer to a disease-causing change, but technically any change, whether it ...
Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
... step would have been diagnosed.
Figure 2 shows some of the minor peaks in the spectrum taken after step 13. Small amounts of deletion
peptides are seen in addition to small peaks representing residual Pbf (Arg-protecting group). These are due to released Pbf attacking, for example, ...
Protein Truncation Tests Using the DCode System
that no normal product is observed in this lane usually indicates a homozygous
mutation. Sequencing of cDNA indicated a deletion
of 174 bp, corresponding
to the entire exon 26. Because this cDNA mutation would result in an in-frame
deletion, the final ...
More siRNA Vectors for RNA Interference
... 6. Kunkel GR and Pederson T (1989)
Transcription of a human U6 small nuclear RNA gene in vivo
of intragenic sequences but not of an upstream
TATATA box. Nucl. Acids Res. 17 , 7371-7379.
7. Ilves H, Barske C, ...
Rapid detection of genomic
duplications and deletions using
the Agilent 2100 bioanalyzer
... the DNA sequence is
altered, (ii) changes of order e.g.
translocations and inversions, and
(iii) quantitative changes, involving
or duplication of a
stretch of DNA. Sequencing has
long been the gold standard for
qualitative changes, and fluorescent
in situ hybridization ...
Cloning Based on Efficient Three-Fragment Assembly
... of them were due to
contamination resulting from incompletely cleaved
and self-ligated phage DNA backbone. A relatively
small proportion of deletion
mutants and clones containing
a doubled phage backbone was also found.
The excellent performance of the ...
Mastercycler Gradient Quick Programming Guide
... YES or NO
A. Position cursor to desired program
press ENTER. Program is now deleted from menu
Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
... three single-tube assay kits, based on molecular
beacon technology, to quickly detect three common human gene mutations:
One is a three-base-pair deletion
in the cystic fibrosis transmembrane
conductance regulator (CFTR) gene, and the other two are single nucleotide
substitutions in the coagulation ...
Production of Full-Length, Biologically Active MEK1WT and MEK1CA
... MEK activators. Substitution of the two phosphoacceptors (Ser-218 and
Ser-222) in MEK1 WT with glutamic acid, in combination with an
(from amino acid 32 to amino acid 51), produced a mutant MEK
that is more active than wild type. 4-6 This mutant, made by
Generate Adenovirus Vectors in E. coli by Homologous Recombination
with the AdEasy Adenoviral Vector System
... cells can be
The most commonly used adenoviral vector, human adenovirus serotype 5, is
rendered replication defective by the deletion
of the E1 and E3 genes. The E1
gene is essential for the assembly of infectious virus particles and is
complemented in vivo by an adenovirus ...