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Complementation in Biological Technology

Odyssey Thera Announces Collaboration With Mitsubishi Tanabe Pharma Corporation, and Issuance of Core Patent in Japan

... number JP 4262778, entitled "Protein fragment complementation assays for the detection of biological or drug ... Thera will apply its patented Protein-fragment complementation Assay (PCA), proprietary High Context Screening ... centers on its proprietary Protein-fragment complementation Assay (PCA) technology and unique High Context ...

Odyssey Thera Granted U.S. Patent for Novel GPCR Assay Strategy

... today that it has been granted a United States patent entitled "Fragment complementation Assays for G-Protein-Coupled Receptors and their Signaling Pathways." The ... Thera's pathway-based approach centers on its proprietary protein-fragment complementation assay (PCA) technology, as well as the considerable body of trade secrets ...

Caliper Life Sciences and DiscoveRx Collaborate to Offer Solutions for Cell-Based GPCR Profiling and Lead Optimization

... combined with DiscoveRx's innovative Beta-Galactosidase Enzyme Fragment complementation (EFC) approach for cellular GPCR assays, should provide researchers with ... The company pioneered the use of Beta-Galactosidase enzyme fragment complementation in biochemical and cell based assays for discovery research, and holds ...

Caliper Life Sciences and DiscoveRx Announce Availability of PathHunter eXpress Reagents for GPCR Assays

... in Fremont, California, with an additional office in Birmingham, England. The Company pioneered the use of Beta-Galactosidase enzyme fragment complementation in biochemical and cell based assays for discovery research, and holds extensive intellectual property in this area. DiscoveRx is dedicated to the ...

Genetic Engineering & Biotechnology News reports on growing use of cell-based assays

... interactions. DiscoveRx created a new family of cell-based assays for the detection of protein-protein interactions using an enzyme fragment complementation assay. This system uses an enzyme acceptor, an inactive alpha-galactosidase enzyme that binds to its enzyme donor forming an active enzyme that will ...

Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases

... and tyrosine kinases. Based on enzyme fragment complementation and the subsequent hydrolysis of chemiluminescent ... binding assays utilizing enzyme fragment complementation (EFC) for the sensitive and simple measurement of ... β-galactosidase (β-gal) fragments; complementation of the enzyme donor (ED) fragment and the enzyme ...

Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3', 5' monophosphate and its validation for pharmacological screening at G protein-coupled receptors

... Key words: cAMP • GPCR • enzyme fragment complementation • LEADseeker • assay miniaturization G ... we describe an assay for screening at GPCRs based on enzyme fragment complementation (EFC) technology and cyclic adenosine 3’, 5’ monophosphate ...

Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library

... clone encoding a novel low-abundance protein called Apobec-1 complementation Factor (ACF) for several important reasons: The simple two-step ... arrays to isolate a full-length clone that encodes Apobec-1 complementation Factor (ACF), a novel RNA-binding protein that is involved in ...

Use of the HitHunter EFC Chemiluminescent Detection Kits with LEADseeker Multimodality Imaging System

... MAPK binding assay kits were used as model systems. The data demonstrate that the HitHunter range of assay kits, which are based on enzyme fragment complementation technology, perform well in conjunction with the chemiluminescent readout of LEADseeker Multimodality Imaging System equipped with the supplied ...

Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System

... and sequence diversity of the Mlo family in plants, J Biol Chem 274, 3499335004 (1999) Shirasu K et al., Cell-autonomous complementation of mlo resistance using a biolistic transient expression system, Plant J 17, 293299 (1999) Zhou F et al., Molecular ...

Generate High-Quality, Directional Plasmid cDNA Libraries

... to create cDNA libraries for these purposes. For functional screening applications, such as yeast two-hybrid screening, complementation studies and expressed sequence tag (EST) sequencing, cDNA libraries constructed directly into plasmid vectors are convenient and ...
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