Navigation Links
Cleavage in Biological Technology

Incorrectly cleaved protein leads to schizophrenia

... Leuven have discovered that a disturbed cleavage of the Nrg-1 protein lies at the basis of the ... after it has been cleaved in the right way. This cleavage is the responsibility of a molecular 'scissors' ... the risk of this disease, results in incorrect cleavage of Nrg-1 by the gamma-secretase. The ...

VisEn Launches Next-Generation Fast ('Fluorescence Activatible Sensor Technology') Agent Platform for Expanded Performance in Imaging Disease Biomarkers In Vivo

... Peterson. According to Dr. Peterson, the FAST platform combines an activation site with two internally quenched fluorophores that fluoresce upon cleavage by MMPs in vivo and are then detected and quantified by the VisEn FMT 2500 in vivo imaging system. VisEn's proprietary FMT technology provides ...

SeqWright to Invest in LaserGen's Novel Lightning Terminators(TM)

... and how well they bind to DNA polymerase. Simply stated, Lightning Terminators(TM) are better than nature. All reversible terminators required a cleavage step to remove the terminating and fluorescent dye groups. Upon cleavage, however, Lightning Terminators(TM) restore the incorporated nucleotide ...

Banyan Biomarkers Announces Issuance of U.S. Patent for Biomarkers for Traumatic Injury

... also has exclusive licenses from the University of Florida for U.S. Patent no. 7,291,710 "Detection of Spectrin and Spectrin Proteolytic cleavage Products in Assessing Nerve Cell Damage" and U.S. Patent No. 7,396,654 "Neural Proteins as Biomarkers for Traumatic Brain Injury." ...

RVX-208 Exploratory Study Illustrates Early Potential for Alzheimer's Disease

... thought to increase the production and accumulation of the putative AD neurotoxin, amyloid-beta (A-beta). The A-beta peptide is produced by aberrant cleavage of a larger precursor protein resulting in two lengths, either 42 or 40 amino acids long. "Stemming from RVX-208's effects on ApoA-I, Prebeta-HDL ...

Stevens researchers provide new information about mass spectrometry

... heterolytic fragmentation mechanism. In contrast, the m/z 121 ion derived from meta and para isomers undergoes a charge-remote homolytic cleavage to eliminate an *H and form a distonic anion radical, which eventually loses CO to produce a peak at m/z 92. In fact, for the para isomer, this ...

Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)

... or fluorophore is attached near the peptides cleavage site and enzymatic activity is detected by the ... for various protease assays. 14 In each case, cleavage of the fluorophore results in increased ... poly(ADPribose) polymerase, in which case the cleavage site is at the tetrapeptide AspGluValAsp (DEVD). ...

RNA Interference and Gene Silencing: History and Overview

... apparently produced in vivo by cleavage of dsRNA introduced directly or via a transgene ... to the introduced dsRNA and that cleavage occurred at 21-23 nucleotide intervals (cleavage events degrade the RNA to 19-21 bp ...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

... that message is then targeted for cleavage leading to gene silencing. 2. ... amount of siRNA required to direct cleavage of the target gene. 3. ... RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell ...

Dicer vs. RNase III for Preparation of siRNA Cocktails

... the enzyme responsible for cleavage of long dsRNAs to siRNA in the ... responsible for digesting long dsRNA, its cleavage products range from ~12 to ... Although RNase III does produce shorter cleavage products than Dicer, these shorter ...

A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage

... (cysteinyl-aspartic acid proteases) followed by cleavage of the caspase substrates [1]. The 113-kDa ... agents. Detection of a 89-kDa or 24-kDa caspase cleavage fragment of PARP-1 was shown to be a hallmark ... With the anti-PARP Caspase cleavage Product Antibody you will specifically detect ...

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

... by the cDNA insert, the thrombin cleavage site, three copies of the c-myc ... The fusion protein also contains a thrombin cleavage site between GFP and the c-myc ... active during protein purification and cleavage (data not shown). Fig.5 ...

Mammalian and Bacterial Expression in One Vector

... In addition, the thrombin protease cleavage site allows cleavage to occur in the presence of thrombin ... experiment showed that successful cleavage was achieved when the purified GFP-CBP ...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

... terminus of the inserted polypeptide sequence, cleavage of fusion proteins produced in the pCAL-n-EK ... acids derived from the fusion tag. Therefore, cleavage results in the production of native protein. ... the CBP coding sequence followed by the EK cleavage site. This configuration ...

Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini

... have no extraneous amino acids derived from the cleavage site. Thus, recombinant proteins with native ... site, (aspartic acid) 4 lysine. This cleavage produces fusion proteins that have ... ). In the past, the use of EK for fusion protein cleavage was hampered by the generally poor ...

Yeast Protein Production System Features High Yields and One-Step Purification

... can be removed from the protein by proteolytic cleavage with either bovine enterokinase or thrombin. ... detached from the GST tag with either protease. cleavage by thrombin results in a fusion ... 4 Removing the GST Tag by Proteolytic cleavage with Enterokinase or Thrombin In many ...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

... codon choice immediately downstream of the EK cleavage site, and thus predetermine the N-terminal ... fused immediately downstream of the EK cleavage site, thereby allowing the recovery of proteins ... affinity tag followed by a thrombin cleavage site, the FLAG epitope, an ...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

... resulting in a seamless junction between the EK cleavage site and the initiation codon (ATG) of the gene ... GST affinity tag may be removed by proteolytic cleavage with EK to yield polypeptides with native amino acid sequence. cleavage with thrombin yields the recombinant protein ...

A New C-Terminal GST Vector for Protein Production in S. pombe

... the GST tag can be removed by proteolytic cleavage with thrombin or enterokinase. Expression and ... a sequence coding for the recognition and cleavage sites for enterokinase and thrombin have been engineered between the MCS and the GST gene. cleavage of the fusion protein with enterokinase results ...

Analysis of a Verapamil Microsomal Incubation using Metabolite ID and Mass FrontierTM

... This metabolite is likely the result of a three step process where alkyl cleavage at the tertiary amine group of verapamil initially produces a previously ... This makes sense because any metabolite resulting from the same alkyl cleavage would most likely correlate better with this reference than with the ...

Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse

... The temperature within the cuvettes was set to 25C to promote cleavage of the peptide linker by trypsin (Figure 2). Using the Scan ... be accurately monitored in cytosolic lysates from yeast cells. cleavage of the BFP-GFP fusion using trypsin (Peltier temperature ...

Efficient Primary Structure Elucidation of Disulfide-bridged Peptides

... motif. The proximity of the cysteine residues and the absence of suitable cleavage sites within the amino-acid sequences of CSTXpeptides caused the classical ... chemical or enzymatic cleavage, they require the presence of defined cleavage sites between two consecutive disulfide bridges. Consequently, the ...

Uracil-DNA Glycosylase

... of dUTP. The apyriminic sites formed by UNG are susceptible to cleavage by heat under alkaline conditions (carry-over prevention). UNG ... the presence of dUTP). Incubation conditions for cleavage of uracil-glycosidic bonds: 20C, 110 min. ...

Characterization of Protein Oligosaccharides Using MSn

... the relatively low abundance of these crossring fragments, and cleavage of especially labile bonds can suppress the formation of other ... 5 H5 5 -II is continuously infused into the instrument. Favorable cleavage sites lie adjacent to GlcNAc residues, particularly terminal ...

Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn

... peptide and the oligosaccharide. The difference in mass following cleavage is used to infer the carbohydrate constituents of the cleaved ... Theoretical m/z values for all possible cleavage products of the glycoform were added to the database to enable ...

Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray Ion Trap Mass Spectrometry

... Typically, this analysis involves Met-specific chemical cleavage (by cyanogen bromide, CNBr) or proteolytic digestion followed by ... mapping of vertebrate CaM. The arrows indicate theoretical cleavage sites for trypsin, while the letters indicate tryptic fragments ...

The Bio-Rad Silver Stain, Rev E

... specific binding, affinity labeling, and relationship to sensitivity Proc Natl Acad Sci USA 82, 57565760 (1985) 39. Laemmli UK, cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680685 (1970) 40. Lee-Huang S, Cloning ...

Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A

... and checking the purity of protein fractions) and performing one-dimensional differential expression studies, degradation assessments, cleavage investigations, and analysis of immunoprecipitation experiments. This tech note presents a comparison of the performance of the ...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

... gradient gel electrophoresis (TGGE), 4 heteroduplex analysis (HA), 5 protein truncation test (PTT), 6 and enzymatic/chemical cleavage analysis, are the major detection methods used to screen mutations. The DCode universal mutation detection system (Figure 1) can ...

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

... M., Anal. Biochem., 72 , 248 (1976). 30. Ornstein, L., Davis, B. J., Anal. NY Acad. Sci., 121 , 321 (1964). 31. Laemmli, U. K., cleavage of structural proteins during the assembly of the head of bacteriophage T, Nature, 680686 (1970). 32. Towbin, H., Staehelin, T., ...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

... codon, ACC, introduces a unique restriction site for the enzyme DraI which is not present in the wild type cDNA sequence. cleavage of the FasIII cDNAs with DraI results in an 368 bp fragment when the polymorphic codon is expressed, while this enzyme does not ...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

... presence of an immature, posttranslationally non-modified form of S-glycoprotein as previously reported (Shoup et al., 1997) or cleavage product. Similarly, a minor protein band in addition to the full size recombinant TGEV-M protein was detected by western blot ...

Nuclease Protection Assays: The Basics

... of the single-stranded, antisense probe must typically be completely homologous to target RNA to prevent cleavage of the probe:target hybrid by nuclease. This means that partially related sequences (e.g., probe and target ...

Northern Analysis: The Basics

... of the data and the ability to quantitate expression are severely compromised. For example, even a single cleavage in 20% of 4 kb target molecules will decrease the returned signal by 20%. Thus, RNase-free reagents and ...

Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA

... 1. Zamore PD, Tuschl T, Sharp Pa, Bartel DP. (2000) RNAi: double stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell. 101(1) : 2533. 2. Elbashir SM, Lendeckel W, and ...

Translate High Yields of Biologically Active Proteins

... a p-nitrophenyl acetate hydrolysis assay [3]. Caspase 3 (CASP3): Catalytic activity was determined by measuring the proteolytic cleavage of the fluorogenic substrate Ac-DEVD-AFC for 1 hr in a 96 well plate reader (400 nm excitation wavelength, 505 nm emission ...

Rapidly Prepare Probes and Markers for Small RNA Analysis

... the phosphorylation reaction is performed with a 150 nt RNA transcript (provided), then diluted into a cleavage reagent that generates the molecular weight marker set in a 5 minute, room temperature reaction. For exact ...

Measuring RNase Activity A Real-time Kinetic Analysis

... mathematical proportionality predicted for steady-state kinetic analysis. Figure 2. Kinetics of RNaseAlert cleavage by RNase A. Using conditions of substracte excess, the rate of increase in fluorescence (the slope of the line) was used to calculate ...

Avoid siRNA Design Altogether!

... 3 nucleotide 3' overhangs, and 5'-phosphate and 3'-hydroxyl termini. The termini and overhangs of RNase III cleavage products are thus the same as those produced by Dicer in the in vivo RNAi pathway. ...
Other Contents
(Date:8/21/2014)... researchers at Louisiana Tech University has developed ... 3D printers and materials to fabricate custom ... chemotherapeutic compounds for targeted drug delivery. , ... research faculty from Louisiana Tech,s biomedical engineering ... filament extruders that can make medical-quality 3D ...
(Date:8/20/2014)... pregnant teenagers in Rochester, NY, nearly half engaged in ... cornstarch, vacuum dust, baby powder and soap, and other ... such teens had significantly lower iron levels as compared ... Pregnant teens, regardless of pica, are at higher risk ... and anemia. Low iron in pregnant teens raises the ...
(Date:8/20/2014)... are visible in in this image of the Para ... these were most likely intentionally set in order to ... forest or stand of trees where the land is ... include conversion of forestland to farms, ranches, or urban ... green of the Amazon Rainforest in the middle of ...
Breaking Biology News(10 mins):Louisiana Tech University researchers use 3D printers to create custom medical implants 2Louisiana Tech University researchers use 3D printers to create custom medical implants 3Pica in pregnant teens linked to low iron 2
(Date:8/21/2014)... TX (PRWEB) August 21, 2014 Improv ... 20 entertained residents with dementia at Silverado Turtle Creek ... Troupe, which consists of entertainers ranging from ages 10 ... time they perform their 40-minute set. The troupe's philosophy ... it universal and allows them to touch audiences regardless ...
(Date:8/21/2014)... THURSDAY, Aug. 21, 2014 (HealthDay News) -- Black mothers ... babies, and here,s one possible reason why: Hospitals in ... nursing than those in areas with more white residents, ... support breast-feeding are much less common in medical centers ... from the U.S. Centers for Disease Control and Prevention ...
(Date:8/21/2014)... Honolulu (PRWEB) August 21, 2014 ... its 10th anniversary and a national voice in ... leaders in health disparities during the 112th National ... , Distinguished honorees included: , John Ruffin, Ph.D, ... Health and Health Disparities, whose life-long career and ...
(Date:8/21/2014)... rural communities do not routinely screen women for ... medical and public health researchers. Rural women who ... if they seek help., "Rural health care providers ... S. McCall-Hosenfeld, a primary care physician and assistant ... State College of Medicine. "However, in rural settings, ...
(Date:8/21/2014)... 2014 The report "Industrial Flue ... Control System (FGD, DeNOx, Particulate Control, Mercury Control) ... Chemical & Others) - Global Trends & Forecasts ... flue gas treatment systems & services market with ... in terms of value. The industrial flue gas ...
Breaking Medicine News(10 mins):Health News:Unicorn Clearance Improv Troupe Brings Laughter to Residents with Dementia at Silverado Memory Care 2Health News:Racial Disparities in Breast-Feeding May Start With Hospitals, Study Suggests 2Health News:W. Montague Cobb/NMA Health Institute Honors Three Outstanding Leaders in Health Disparities 2Health News:Primary care physicians can be critical resource for abused women in rural areas 2Health News:Industrial Flue Gas Treatment Systems Market Estimated to Worth $59, 546.7 Million by 2019 – Report by MarketsandMarkets 2Health News:Industrial Flue Gas Treatment Systems Market Estimated to Worth $59, 546.7 Million by 2019 – Report by MarketsandMarkets 3Health News:Industrial Flue Gas Treatment Systems Market Estimated to Worth $59, 546.7 Million by 2019 – Report by MarketsandMarkets 4
Other TagsOther Tags