... Leuven have discovered that a disturbed
of the Nrg-1 protein lies at the basis of the ... after it has been cleaved in the right way. This
is the responsibility of a molecular 'scissors' ... the risk of this disease, results in incorrect
of Nrg-1 by the gamma-secretase.
... Peterson. According to Dr. Peterson, the FAST platform combines an activation site with two internally quenched fluorophores that fluoresce upon
by MMPs in vivo and are then detected and quantified by the VisEn FMT 2500 in vivo imaging system. VisEn's proprietary FMT technology provides ...
... and how well they bind to DNA polymerase. Simply stated, Lightning Terminators(TM) are better than nature. All reversible terminators required a
step to remove the terminating and fluorescent dye groups. Upon cleavage, however, Lightning Terminators(TM) restore the incorporated nucleotide ...
... also has exclusive licenses from the University of Florida for U.S. Patent no. 7,291,710 "Detection of Spectrin and Spectrin Proteolytic
Products in Assessing Nerve Cell Damage" and U.S. Patent No. 7,396,654 "Neural Proteins as Biomarkers for Traumatic Brain Injury."
... thought to increase the production and accumulation
of the putative AD neurotoxin, amyloid-beta (A-beta). The A-beta peptide is
produced by aberrant
of a larger precursor protein resulting in
two lengths, either 42 or 40 amino acids long.
"Stemming from RVX-208's effects on ApoA-I, Prebeta-HDL ...
... heterolytic fragmentation mechanism.
In contrast, the m/z 121 ion derived from meta and para isomers undergoes a charge-remote homolytic
to eliminate an *H and form a distonic anion radical, which eventually loses CO to produce a peak at m/z 92. In fact, for the para isomer, this ...
... or fluorophore is attached near the peptides
site and enzymatic activity is detected by the ... for various protease assays. 14 In each case,
of the fluorophore results in increased ... poly(ADPribose) polymerase, in which case the
site is at the tetrapeptide AspGluValAsp (DEVD). ...
... apparently produced in vivo
of dsRNA introduced directly or via a transgene ... to the introduced dsRNA and
that cleavage events
degrade the RNA to 19-21 bp ...
Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect... that message is then targeted
for cleavage leading to gene silencing.
2. ... amount of siRNA
required to direct cleavage of the target gene.
3. ... RNA directs the ATP-dependent
cleavage of mRNA at 21 to 23 nucleotide intervals. Cell ...
Dicer vs. RNase III for Preparation of siRNA Cocktails... the
enzyme responsible for cleavage of long dsRNAs to siRNA in the
... responsible for digesting long dsRNA, its cleavage products
range from ~12 to ... Although RNase III does produce shorter cleavage products
than Dicer, these shorter ...
A Further Step in Understanding Apoptosis
Direct Detection of PARP Cleavage... (cysteinyl-aspartic
acid proteases) followed by cleavage of the caspase substrates
. The 113-kDa ... agents. Detection of a 89-kDa or 24-kDa
caspase cleavage fragment of PARP-1 was shown to be
a hallmark ...
With the anti-PARP Caspase cleavage Product Antibody
you will specifically detect ...
Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression
by the cDNA insert, the thrombin cleavage site, three copies of the c-myc
... The fusion protein also contains a thrombin cleavage site between GFP
and the c-myc ... active during protein purification and
cleavage (data not shown).
Mammalian and Bacterial Expression in One Vector... In addition, the thrombin
protease cleavage site allows cleavage to occur in the presence of thrombin
experiment showed that successful cleavage was achieved when the purified
Expression and Purification of Recombinant Proteins That Have Native Amino
Acid Sequence... terminus of the inserted polypeptide
sequence, cleavage of fusion proteins produced in the pCAL-n-EK ... acids derived from the
fusion tag. Therefore, cleavage results in the production of native protein.
... the CBP
coding sequence followed by the EK cleavage site. This configuration ...
Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini... have no
extraneous amino acids derived from the cleavage site. Thus, recombinant
proteins with native ... site, (aspartic
acid) 4 lysine. This cleavage produces fusion proteins that have
... ). In the past, the use of EK for fusion protein cleavage was hampered
by the generally poor ...
Yeast Protein Production System Features High Yields and One-Step
Purification... can be removed from
the protein by proteolytic cleavage with either bovine enterokinase or thrombin.
... detached from the GST tag with either protease. cleavage by
thrombin results in a fusion ... 4
Removing the GST Tag by Proteolytic cleavage with Enterokinase or Thrombin
In many ...
High-Level Protein Expression, One-Column Purification, and FLAG
Epitope Tagging in E. coli... codon choice immediately downstream of the EK cleavage site, and thus
predetermine the N-terminal ... fused immediately downstream of
the EK cleavage site, thereby allowing the recovery of proteins ... affinity tag followed by a thrombin
cleavage site, the FLAG epitope, an ...
New Yeast Cloning System for Producing Proteins with Native Amino Acid
Sequences... resulting in a
seamless junction between the EK cleavage site and the initiation codon (ATG) of
the gene ... GST affinity tag may be removed by proteolytic cleavage with EK
to yield polypeptides with native amino acid sequence. cleavage with thrombin
yields the recombinant protein ...
A New C-Terminal GST Vector for Protein Production in S. pombe... the GST tag can be removed by
proteolytic cleavage with thrombin or enterokinase.
Expression and ... a sequence coding for the
recognition and cleavage sites for enterokinase and thrombin have been
engineered between the MCS and the GST gene. cleavage of the fusion protein with
enterokinase results ...
Analysis of a Verapamil Microsomal Incubation using Metabolite ID and Mass FrontierTM... This metabolite is likely the result of a three step process where alkyl cleavage at the tertiary amine group of verapamil initially produces a previously ... This makes sense because any metabolite resulting from the same alkyl cleavage would most likely correlate better with this reference than with the ...
Monitoring fluorescence resonance energy transfer (FRET) between GFP
fusions in lysates of the yeast Saccharomyces cerevisiae using the
Cary Eclipse... The temperature within the cuvettes was set to 25C
to promote cleavage of the peptide linker by trypsin (Figure 2). Using the Scan
... be accurately monitored in cytosolic
lysates from yeast cells. cleavage of the BFP-GFP fusion using trypsin (Peltier
Efficient Primary Structure Elucidation of Disulfide-bridged Peptides... motif. The proximity of the cysteine residues and the absence of suitable cleavage sites within the amino-acid sequences of CSTXpeptides caused the classical ... chemical or enzymatic cleavage, they require the presence of defined cleavage sites between two consecutive disulfide bridges. Consequently, the ...
of dUTP. The apyriminic sites formed by UNG are susceptible to cleavage
by heat under alkaline conditions (carry-over prevention). UNG ... the presence of dUTP).
Incubation conditions for cleavage of uracil-glycosidic
bonds: 20C, 110 min.
Characterization of Protein Oligosaccharides Using MSn... the relatively low abundance of these crossring
fragments, and cleavage of especially labile bonds
can suppress the formation of other ... 5 H5 5 -II is continuously infused into the
instrument. Favorable cleavage sites lie adjacent to GlcNAc residues, particularly
Structural Analysis of Glycosylated Peptides in Complex Mixtures with
Ion Trap MSn... peptide and the oligosaccharide. The difference
in mass following cleavage is used to infer the carbohydrate constituents
of the cleaved ...
Theoretical m/z values for all possible cleavage
products of the glycoform were added to the database to enable ...
Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray
Ion Trap Mass Spectrometry... Typically, this analysis involves Met-specific chemical
cleavage (by cyanogen bromide, CNBr) or proteolytic digestion followed by
... mapping of vertebrate CaM. The arrows indicate theoretical
cleavage sites for trypsin, while the letters indicate tryptic fragments
The Bio-Rad Silver Stain, Rev E... specific binding, affinity labeling, and relationship to sensitivity
Proc Natl Acad Sci USA 82, 57565760 (1985)
39. Laemmli UK, cleavage of structural proteins during the assembly of
the head of bacteriophage T4, Nature 227, 680685 (1970)
40. Lee-Huang S, Cloning ...
Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A... and checking the purity of protein fractions) and performing one-dimensional
differential expression studies, degradation assessments, cleavage investigations,
and analysis of immunoprecipitation experiments.
This tech note presents a comparison of the performance of the ...
Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations... gradient gel electrophoresis (TGGE), 4 heteroduplex analysis
(HA), 5 protein truncation test (PTT), 6 and enzymatic/chemical cleavage
analysis, are the major detection methods used to screen mutations.
The DCode universal mutation detection system (Figure 1) can ...
Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing... M., Anal. Biochem., 72 , 248 (1976).
30. Ornstein, L., Davis, B. J., Anal. NY Acad. Sci., 121 , 321 (1964).
31. Laemmli, U. K., cleavage of structural proteins during the assembly
of the head of bacteriophage T, Nature, 680686 (1970).
32. Towbin, H., Staehelin, T., ...
Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis... codon, ACC, introduces a unique restriction
site for the enzyme DraI which is not present in the wild type
cDNA sequence. cleavage of the FasIII cDNAs with DraI
results in an 368 bp fragment when the polymorphic codon
is expressed, while this enzyme does not ...
Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A... presence of an immature, posttranslationally
non-modified form of S-glycoprotein as previously reported
(Shoup et al., 1997) or cleavage product. Similarly, a minor
protein band in addition to the full size recombinant TGEV-M
protein was detected by western blot ...
Nuclease Protection Assays: The Basics... of the
single-stranded, antisense probe must typically be completely
homologous to target RNA to prevent cleavage of the
probe:target hybrid by nuclease. This means that partially
related sequences (e.g., probe and target ...
Northern Analysis: The Basics... of the data
and the ability to quantitate expression are severely
compromised. For example, even a single cleavage in 20% of 4
kb target molecules will decrease the returned signal by 20%.
Thus, RNase-free reagents and ...
Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA... 1. Zamore PD, Tuschl T, Sharp
Pa, Bartel DP. (2000) RNAi: double stranded RNA directs the
ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals.
Cell. 101(1) : 2533.
2. Elbashir SM, Lendeckel W, and
Translate High Yields of Biologically Active Proteins... a p-nitrophenyl
acetate hydrolysis assay . Caspase 3 (CASP3): Catalytic activity was
determined by measuring the proteolytic cleavage of the fluorogenic substrate
Ac-DEVD-AFC for 1 hr in a 96 well plate reader (400 nm excitation wavelength,
505 nm emission ...
Rapidly Prepare Probes and Markers for Small RNA Analysis... the phosphorylation
reaction is performed with a 150 nt RNA transcript (provided),
then diluted into a cleavage reagent that generates the
molecular weight marker set in a 5 minute, room temperature
reaction. For exact ...
Measuring RNase Activity A Real-time Kinetic Analysis... mathematical proportionality predicted for steady-state kinetic
Figure 2. Kinetics of RNaseAlert cleavage by RNase A. Using conditions of substracte excess, the
rate of increase in fluorescence (the slope of the line) was used to calculate
Avoid siRNA Design Altogether!... 3 nucleotide 3'
overhangs, and 5'-phosphate and 3'-hydroxyl termini. The
termini and overhangs of RNase III cleavage products are thus
the same as those produced by Dicer in the in vivo RNAi