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Chill in Biological Technology

Cool! Nanoparticle research points to energy savings

... Adding just the right dash of nanoparticles to standard mixes of lubricants and refrigerants could yield the equivalent of an energy-saving chill pill for factories, hospitals, ships, and others with large cooling systems, suggest the latest results from National Institute of Standards and ...

'Radio wave cooling' offers new twist on laser cooling

... cooled it from room temperature (about 23 degrees C, or 73 degrees F) to -228 C (-379 F). Other research groups have used optical techniques to chill micro-cantilevers to lower temperatures, but the RF technique may be more practical in some cases, because the equipment is smaller and easier to ...

Sample Preparation for Microinjection

... for 15 minutes at 4C (10,000 x g). chill supernatant or load directly into ... 15 minutes at 4C (10,000 x g). chill supernatant or load directly into ... 15 minutes at 4C (10,000 x g). chill supernatant or load directly into ...

CIOs could keep companies out of hot water in court

... Fusion2006 CEO-CIO Symposium on Thursday. Morgan Stanley's $1.4 billion example in 2005 of how not to produce documents in a court case has put a chill in the hearts of many company officers. The case was a relatively ordinary one, said Paul Benson, a corporate lawyer with Michael Best & ...

Salmonella typhimurium

... 1. Incoculate a flask of LB 1:100 with a fresh overnight culture, grow at 37 C with shaking to an O.D. 640 of 0.75. chill the cells in an ice-water bath for 15 min. 2. Harvest by centrifugation (4,000 x g, 10 ...

Pseudomonas aeruginosa

... solution. 4. Resuspend in 300 mM sucrose to a final concentration of 10 11 cells/ml, chill on ice for 30 minutes. Electroporation of cells: ...

Neurospora crassa

... sorbitol / 1% PEG. Resuspend the protoplasts in 0.5 ml ice-cold electroporation buffer (2 x 108 protoplasts/ml), chill on ice for 10 minutes. Electroporation of protoplasts Add 4 l plasmid DNA (0.5 mg/ml) to 400 ...

Neurospora crassa

... sorbitol / 1% PEG. Resuspend the protoplasts in 0.5 ml ice-cold electroporation buffer (2 x 10 8 protoplasts/ml), chill on ice for 10 minutes. Electroporation of cells Add 4 l plasmid DNA (0.5 mg/ml) ...

Escherichia coli DH5

... culture of E. coli DH5alpha. Grow at 37 C with shaking to an O.D. 600 of 0.5 to 0.6. 2. chill cells on ice for 15 minutes and transfer to a prechilled centrifuge bottle. Harvest by centrifugation (20 minutes, 5,000 ...

Escherichia coli DH10B

... culture of E. coli DH10B. Grow at 37 C with shaking to an O.D. 600 of 0.5 to 0.6. 2. chill cells on ice for 15 minutes and transfer to a prechilled centrifuge bottle. Harvest by centrifugation (20 minutes, 5,000 ...

Escherichia coli K12

... culture of E. coli DH10B. Grow at 37 C with shaking to an O.D. 600 of 0.5 to 0.6. 2. chill cells on ice for 15 minutes and transfer to a prechilled centrifuge bottle. Harvest by centrifugation (20 minutes, 5,000 ...

Escherichia coli C600

... culture of E. coli C600. Grow at 37 C with shaking to an O.D. 600 of 0.5 to 0.6. 2. chill cells on ice for 15 minutes and transfer to a prechilled centrifuge bottle. Harvest by centrifugation (20 minutes, 5,000 ...

Brucella abortus

... Electroporation of cells: Grow cells in Trypticase soy broth at 37 C with vigorous shaking, chill on ice for 10 min. Harvest by centrifugation (10 min, 10,000 x g). Wash three times in an equal volume of ...

Bifidobacterium animalis

... Grow cells overnight at 37 C. Dilute this culture 1:25 in fresh medium and cultivate at 37 C until an O.D. 695 of 0.2. chill bacteria on ice. 2. Harvest by centrifugation. 3. Wash ...

Actinomyces viscosus

... Grow a 500 ml cell culture in the early exponential phase until an O.D. 600 of 0.2 is reached. chill on ice for 1.5 h. 2. Harvest by centrifugation. Wash extensively first with a large volume of ...

Salmonella typhimurium

... 1. Incoculate a flask of LB 1:100 with a fresh overnight culture, grow at 37 C with shaking to an O.D. 640 of 0.75. chill the cells in an ice-water bath for 15 min. 2. Harvest by centrifugation (4,000 x g, 10 ...

Pseudomonas aeruginosa

... solution. 4. Resuspend in 300 mM sucrose to a final concentration of 10 11 cells/ml, chill on ice for 30 minutes. Electroporation of cells: ...

Escherichia coli K12

... culture of E. coli DH10B. Grow at 37 C with shaking to an O.D. 600 of 0.5 to 0.6. 2. chill cells on ice for 15 minutes and transfer to a prechilled centrifuge bottle. Harvest by centrifugation (20 minutes, 5,000 ...

Escherichia coli DH5

... culture of E. coli DH5alpha. Grow at 37 C with shaking to an O.D. 600 of 0.5 to 0.6. 2. chill cells on ice for 15 minutes and transfer to a prechilled centrifuge bottle. Harvest by centrifugation (20 minutes, 5,000 ...

Escherichia coli DH10B

... culture of E. coli DH10B. Grow at 37 C with shaking to an O.D. 600 of 0.5 to 0.6. 2. chill cells on ice for 15 minutes and transfer to a prechilled centrifuge bottle. Harvest by centrifugation (20 minutes, 5,000 ...

Escherichia coli C600

... culture of E. coli C600. Grow at 37 C with shaking to an O.D. 600 of 0.5 to 0.6. 2. chill cells on ice for 15 minutes and transfer to a prechilled centrifuge bottle. Harvest by centrifugation (20 minutes, 5,000 ...

Brucella abortus

... Electroporation of cells: Grow cells in Trypticase soy broth at 37 C with vigorous shaking, chill on ice for 10 min. Harvest by centrifugation (10 min, 10,000 x g). Wash three times in an equal volume of ...

Bifidobacterium animalis

... Grow cells overnight at 37 C. Dilute this culture 1:25 in fresh medium and cultivate at 37 C until an O.D. 695 of 0.2. chill bacteria on ice. 2. Harvest by centrifugation. 3. Wash ...

Actinomyces viscosus

... Grow a 500 ml cell culture in the early exponential phase until an O.D. 600 of 0.2 is reached. chill on ice for 1.5 h. 2. Harvest by centrifugation. Wash extensively first with a large volume of ...

Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel

... total RNA with 100 ng of oligo-dT-primer for first strand synthesis to 75C for 10 minutes in a reaction volume of 18.5 l. chill tube on ice for 5 minutes and spin. Add 2.5 l dNTPs (2 mM), 2.5 l 10X RT-buffer and 1.5 l Reverse Transcriptase (300 U) to ...

TripleMaster PCR System

... the cycler. Eppendorf 0.2 ml PCR tubes are recommended for the Eppendorf Mastercycler. - Always prepare two master mixes and chill them on ice. - Set up the complete reactions on ice. - Mix the final reaction by gently pipetting up and down (three times). ...

TripleMaster PCR System

... the cycler. Eppendorf 0.2 ml PCR tubes are recommended for the Eppendorf Mastercycler. - Always prepare two master mixes and chill them on ice. - Set up the complete reactions on ice. - Mix the final reaction by gently pipetting up and down (three times). ...
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