OncoGenex Reports Financial Results for Fourth Quarter and Fiscal Year 2008 and Provides Outlook for 2009
... of a family of
compounds known as caspase
activators presently in preclinical
research. As the caspase
family of proteases plays essential roles in
apoptosis, the caspase
activators offer the potential for the
OncoGenex Reports Third Quarter Financial Results
... of a family of compounds known as caspase
activators presently in preclinical research. As the caspase
family of proteases plays essential roles in apoptosis, the caspase
activators offer the potential for the ...
Sonus Pharmaceuticals and OncoGenex Technologies complete business combination; OncoGenex Pharmaceuticals to commence trading on NASDAQ Capital Market today
... the maximum tolerated dose;
- CSP-9222 is a caspase
activator presently in pre-clinical development. caspase
activators consist of small molecules that have ... development within 12-18 months. The caspase
program was in-licensed in August 2008 ...
Ascenta Therapeutics Announces Results of Preclinical Evaluation of AT-406 in Multiple Cancer Models
... is thought to mimic the activity of Smac (second mitochondria-derived activator of caspases) by binding to XIAP and preventing it from inhibiting caspase
activation. Upon binding, AT-406 induces rapid degradation of cIAP-1/2 proteins and promotes apoptosis through activation of caspase-8 and the ...
Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
... Correlated measurement of an apoptosis marker (or caspase
and cell viability
Promega Apo-ONE Homogeneous caspase
3/7 Assay, catalog no. G7790
... of 590 nm with the PHERAstar.
reagents were then added to the plates with the ...
Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)
... Purification and catalytic properties of human caspase
family members. Cell Death and Differentiation ... Kaufmann and W.C. Earnshaw. 1997. Caspases and caspase
inhibitors. Trends Biochem. Sci. 22: 388393. ... 1997. Fluorometric and colorimetric detection of caspase
activity associated with apoptosis. Anal. ...
Use of the HitHunter EFC Chemiluminescent Detection Kits with LEADseeker Multimodality Imaging System
... the LEADseeker Multimodality Imaging System. The caspase
3 and p38 MAPK binding assay kits were used as ... 18-1140-71
HitHunter caspase 3 EFC Chemiluminescent 90-0028-02 ... by the kit manufacturer.
1.2 Prepare caspase 3 enzyme standard curve working dilutions in ...
Translate High Yields of Biologically Active Proteins
... and incubated for 1 hr at 37C. Two proteins, caspase
3 and MAPKinase
9, were synthesized in ... ActivePro Scale-Up Reactions.
3 and MAPKinase9 DNA templates were ... 1 (AK1),
carbonic anhydrase 2 (CA2), caspase
3 (CASP3), and casein kinase 1 (CK1).
Recommendations for Successful siRNA Library Screens
... five different siRNAs
found to block caspase
3 activation (a marker for apoptosis) in a ... cells
were lysed and monitored for caspase
3 activity. Results for each well
are graphed relative to the average caspase
3 activity from a negative
Are siRNA Pools Smart?
... using etoposide one day after transfection, and caspase
was assayed three days ... here to enlarge]
Figure 4. caspase
3 Activation--Single siRNAs
vs. siRNA ... the cells
were counted and lysed. The caspase
3 activity in the lysates was assayed
Apoptosis detection by annexin V and
active caspase-3 with the Agilent 2100
... the effector arm of the
apoptotic program6. caspase
can be achieved by ... In the mitochondrial-
initiated pathway, caspase
is triggered by the formation
of a ... % NaN3)
Protocol for caspase
1. Treat 5 x ...
A Further Step in Understanding Apoptosis
Direct Detection of PARP Cleavage
... proteases) followed by cleavage of the caspase
. The 113-kDa poly(ADP-ribose) ... cellular events, is cleaved by members
of the caspase
family during early apoptosis. The ... apoptosis . In contrast to measuring
3, which is degraded during apoptosis via
James Thomson plans to stay in Madison to study genesis of stem cells
... they dont die easily. Viable embryonic stem cells have high levels of caspase
3/7, enzymes that break down proteins as part of a process called ... of the body. Yet the stem cells do not die, Thomson said, even though the caspase
levels would kill most other cells. The reason is unknown.
Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells
let-7a, and miR-196, known to regulate expression of G6PD , caspase
3 , and HOXB8 , respectively. To find an informative cell ... with a negative control Pre-miR. Expression of G6PD (miR-1b) and caspase
3 (let-7a) were measured using enzymatic assays specific to the ...
Caspase-3 Activation - An Indicator of Apoptosis in Image-Based Assays
We explored the use of the active form of caspase-3 for the detection of apoptotic events. This protease has been implicated as an effector caspase
associated with the initiation of the death cascade and is therefore an important marker of the cells entry point into the apoptotic signaling ...
The measurement of DNA condensation and mitochondrial changes during apoptosis and necrosis in cell based assays
... diclofenac for 4- and 21-h respectively. Staurosporine is a microbial alkaloid, a strong inhibitor of protein kinases, thought to work through JNK1, caspase
proteases, AP-1, and NFκβ. It is an apoptotic inducer causing nuclear condensation (1, 2). Diclofenac is a non-steroidal anti-inflammatory ...
Perform RNAi Library Screens on Any Budget
... to 25 M etoposide (Sigma) 24 hr post transfection. At 72 hr post transfection,
levels of cellular apoptosis were monitored by assaying for caspase
Silencer CellReady siRNA Libraries
Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
... would be to confirm the results with a second
siRNA targeting survivin, and to monitor apoptosis by additional assays
activity assay, annexin V assay, etc.), while continuing
to monitor the extent of survivin mRNA knockdown by qRT-PCR.