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Caspase in Biological Technology

OncoGenex Reports Financial Results for Fourth Quarter and Fiscal Year 2008 and Provides Outlook for 2009

... of a family of compounds known as caspase activators presently in preclinical research. As the caspase family of proteases plays essential roles in apoptosis, the caspase activators offer the potential for the ...

OncoGenex Reports Third Quarter Financial Results

... of a family of compounds known as caspase activators presently in preclinical research. As the caspase family of proteases plays essential roles in apoptosis, the caspase activators offer the potential for the ...

Sonus Pharmaceuticals and OncoGenex Technologies complete business combination; OncoGenex Pharmaceuticals to commence trading on NASDAQ Capital Market today

... the maximum tolerated dose; - CSP-9222 is a caspase activator presently in pre-clinical development. caspase activators consist of small molecules that have ... development within 12-18 months. The caspase program was in-licensed in August 2008 ...

Ascenta Therapeutics Announces Results of Preclinical Evaluation of AT-406 in Multiple Cancer Models

... is thought to mimic the activity of Smac (second mitochondria-derived activator of caspases) by binding to XIAP and preventing it from inhibiting caspase activation. Upon binding, AT-406 induces rapid degradation of cIAP-1/2 proteins and promotes apoptosis through activation of caspase-8 and the ...

Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar

... Correlated measurement of an apoptosis marker (or caspase activity) and cell viability ... Promega Apo-ONE Homogeneous caspase 3/7 Assay, catalog no. G7790 ... of 590 nm with the PHERAstar. The caspase reagents were then added to the plates with the ...

Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)

... Purification and catalytic properties of human caspase family members. Cell Death and Differentiation ... Kaufmann and W.C. Earnshaw. 1997. Caspases and caspase inhibitors. Trends Biochem. Sci. 22: 388393. ... 1997. Fluorometric and colorimetric detection of caspase activity associated with apoptosis. Anal. ...

Use of the HitHunter EFC Chemiluminescent Detection Kits with LEADseeker Multimodality Imaging System

... the LEADseeker Multimodality Imaging System. The caspase 3 and p38 MAPK binding assay kits were used as ... 18-1140-71

HitHunter caspase 3 EFC Chemiluminescent 90-0028-02 ... by the kit manufacturer.

1.2 Prepare caspase 3 enzyme standard curve working dilutions in ...

Translate High Yields of Biologically Active Proteins

... and incubated for 1 hr at 37C. Two proteins, caspase 3 and MAPKinase 9, were synthesized in ... ActivePro Scale-Up Reactions. caspase 3 and MAPKinase9 DNA templates were ... 1 (AK1), carbonic anhydrase 2 (CA2), caspase 3 (CASP3), and casein kinase 1 (CK1). ...

Recommendations for Successful siRNA Library Screens

... five different siRNAs found to block caspase 3 activation (a marker for apoptosis) in a ... cells were lysed and monitored for caspase 3 activity. Results for each well are graphed relative to the average caspase 3 activity from a negative control ...

Are siRNA Pools Smart?

... using etoposide one day after transfection, and caspase 3 activity was assayed three days ... here to enlarge] Figure 4. caspase 3 Activation--Single siRNAs vs. siRNA ... the cells were counted and lysed. The caspase 3 activity in the lysates was assayed ...

Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer

... the effector arm of the apoptotic program6. caspase activation can be achieved by ... In the mitochondrial- initiated pathway, caspase activation is triggered by the formation of a ... % NaN3) Protocol for caspase 3 assay 1. Treat 5 x ...

A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage

... proteases) followed by cleavage of the caspase substrates [1]. The 113-kDa poly(ADP-ribose) ... cellular events, is cleaved by members of the caspase family during early apoptosis. The ... apoptosis [2]. In contrast to measuring active caspase 3, which is degraded during apoptosis via the ...

James Thomson plans to stay in Madison to study genesis of stem cells

... they dont die easily. Viable embryonic stem cells have high levels of caspase 3/7, enzymes that break down proteins as part of a process called ... of the body. Yet the stem cells do not die, Thomson said, even though the caspase levels would kill most other cells. The reason is unknown. Once ...

Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells

... let-7a, and miR-196, known to regulate expression of G6PD [3], caspase 3 [2], and HOXB8 [4], respectively. To find an informative cell ... with a negative control Pre-miR. Expression of G6PD (miR-1b) and caspase 3 (let-7a) were measured using enzymatic assays specific to the ...

Caspase-3 Activation - An Indicator of Apoptosis in Image-Based Assays

... We explored the use of the active form of caspase-3 for the detection of apoptotic events. This protease has been implicated as an effector caspase associated with the initiation of the death cascade and is therefore an important marker of the cells entry point into the apoptotic signaling ...

The measurement of DNA condensation and mitochondrial changes during apoptosis and necrosis in cell based assays

... diclofenac for 4- and 21-h respectively. Staurosporine is a microbial alkaloid, a strong inhibitor of protein kinases, thought to work through JNK1, caspase proteases, AP-1, and NFκβ. It is an apoptotic inducer causing nuclear condensation (1, 2). Diclofenac is a non-steroidal anti-inflammatory ...

Perform RNAi Library Screens on Any Budget

... to 25 M etoposide (Sigma) 24 hr post transfection. At 72 hr post transfection, levels of cellular apoptosis were monitored by assaying for caspase 3 activity. Silencer CellReady siRNA Libraries Convenientpre-aliquotted siRNAs, ...

Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success

... would be to confirm the results with a second siRNA targeting survivin, and to monitor apoptosis by additional assays (e.g., caspase activity assay, annexin V assay, etc.), while continuing to monitor the extent of survivin mRNA knockdown by qRT-PCR. ...
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