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Buffer in Biological Technology

LI-COR Blot Washer Automates Western Blot Processing

... dispense valve on the LI-COR Blot Washer ads flexibility to protocols. For example automated delivery of a secondary antibody or second wash buffer can be established as part of a pre-established wash cycle. The system includes a 140 ml syringe to dispense a secondary antibody and the auxiliary ...

Fight Melanoma, With Joe Pesci and Dennis Haysbert on Monday, April 21st

... Harold Greene (CBS-LA), Lorenzo Neal (NFL-Chargers Pro Bowler), Patrick Warburton (Rules of Engagement), Joe Mantegna (Criminal Minds), Bruce buffer (Voice of the UFC Octagon), Lincoln Kennedy (NFL-Raiders), Glynn Turman (The Wire), Brian Dunkleman (Celebrity Fit Club), Gregory Itzin (24), Shawn ...

Nanosoftball made of DNA

... of a solid-phase synthesis. The three DNA strands were always attached by way of an aromatic six-membered carbon ring. When mixed in equal parts in a buffer solution, these building blocks do aggregate to form the expected product: regular dodecahedra. Atomic force microscopy images reveal them to be ...

BoVir(R) Named Best New Veterinary Diagnostics Product in International Animal Pharm Awards

... early detection of the virus, and it produces the most advanced genotype and strain diversity detection. "Our unique, proprietary lysis buffer eliminates the need for the costly, labor-intensive, and time-consuming RNA extraction step, which further eliminates the complicated error-prone ...

Fundamentals of Fermentation: Techniques For Benchtop Fermentors

... in the solution of 3 molar KC1 or a buffer solution between pH 4 and pH 7. The two ... measuring system is calibrated using two external buffer solutions of known pH. 4. Immerse pH electrode into pH 7.0 buffer solution and allow a few minutes for the system ...

Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column

... in mobile phase composition, such as buffer type and pH, salt concentration, the addition ... was investigated. Concurrently, the buffer concentration was reduced to a minimum buffering ... 250 pores. The original running buffer consisted of 0.1M NaH 2 PO 4 (pH 6.0) ...

Denaturing HPLC (DHPLC)

... • HPLC grade acetonitrile • buffer A, comprising of 100 mM TEAA to pH 7.0, 0.1 mM EDTA, • buffer B, comprising of 100 mM TEAA to pH 7.0, 0.1mM ... flow rate of 0.45 ml/minute Percentage of buffer A, when time is 0.00 minute – 55% ...

QIAGEN Fast Cycling PCR Kit

... handling optional ready-to-load PCR buffer and no primer redesign required ... using a novel, patentpending PCR buffer formulation that significantly reduces the time ... with the new QIAGEN Fast Cycling PCR buffer promotes specific primertemplate annealing ...

QIAGEN LongRange PCR and 2Step RT-PCR Kits

... mix Minimal PCR optimization due to unique buffer system Amplification of difficult ... than Taq DNA polymerase). The novel buffer system provides highly specific ... targets (Figure 2). The LongRange RT buffer is specially formulated to provide efficient ...

HEK293 Calcium Flux Assay

... cell pellet by flicking. Add 1 mL of cell wash buffer (see Section V) with pipette and triturate vigorously to break cell clumps. Add 6 mL of cell wash buffer and triturate vigorously again. Repeat ... Resuspend cell pellet in 1 mL of storage buffer (see Section V) and triturate vigorously to break ...

Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000

... Plate. A Gripper removes Filter Plate A. Trapping buffer is added using standard pipette tips to the ... DNA is further purified by filtering Diluted Wash buffer through the BAC Filter Plate using positive ... plate and the BAC DNA was eluted using Elution buffer using positive pressure. ...

Calcium Flux Assays using the LabChip 3000

... of cell line, growth conditions, and cell assay buffer is made (Step 1). A protocol for labeling, ... If the assay is not stable, then optimization of buffer conditions and cell growth conditions will be ... 1. Choose Cell Line, Cell Growth and Cell buffer Conditions A microfluidic assay using ...

Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation

... for the assay 9 was calculated using the buffer addition (negative control) and the 30 M ATP ... Life Technologies, cat # 14065-049), HEPES 1M buffer solution (Invitrogen Life Technologies, cat # ... Step 1 Wash buffer: this buffer contains a final concentration of 1X HBSS and 20 ...

Gq-coupled Receptor Assays Using FLEXstation

... cat# 14065056; Tel: 1-800-828-6686), HEPES 1M buffer solution (Gibco, cat#15630-080). ... Cell Loading Procedure 1X Reagent buffer was prepared (1X HBSS/20 mM HEPES, pH 7.4) with the reagents described above. Loading buffer was prepared as described on the Molecular ...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

... The contents include: Luciferase assay buffer II (E1955); Luciferase assay substrate (E151A), Stop & Glo buffer (E197A); Stop & Glo substrate (E193B); Stop & Glo ... 1. Passive lysis buffer was prepared by mixing 2 mL of passive lysis 5x ...

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

... a 96-well format. 1. The enzyme and buffer solutions were thawed and kept on ice. 2. TE buffer (10 mM Tris and 1 mM EDTA in sterile, deionized ... by adding 12 mL of L/L Reconstitution buffer to the vial, gently inverting several times and ...

PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

... a clean quartz cuvette containing your sample buffer or distilled water into the SPECTRAmax PLUS ... (5) are displayed in their respective fields. The buffer blanks were assigned to wells D6 and D7. ... plate. Aliquots of each column fraction and the buffer blank were transferred into their designated ...

Coated Plate Immunoassay on the MicroBeta - eg. TSH - IRMA

... *COATING PROCEDURE 1. Coating buffer formulation: 50 mM carbonate buffer, ... 90 ml 2. Add anti-TSH into coating buffer (antibody concentration 1.0 ... containing 0.5% BSA Saturation buffer formulation: 50 mM Tris = 0.606 g ...

Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor

... at room temperature. After 2 washes with binding buffer (128 mM NaCl; 5 mM KCl; 5 mM MgSO4; 1.3 mM CaCl2; ... with blocking solution consisting of binding buffer containing 5% BSA. After incubation, the wells were washed three times with binding buffer containing 1% BSA. For the binding ...

Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor

... membrane protein concentration in 25 mM HEPES buffer containing 2.5 mM CaCl2 and 1 mM MgCl2, at pH ... use, 250 μl of 0.5% BSA in the above HEPES buffer was added to each well and left for 30 minutes at ... method, with some modifications. The same assay buffer was used for both methods. 50 μl ...

Counting Cytostar-T scintillating microplates on the MicroBeta JET

... were prepared in assay buffer, consisting of NMG buffer containing 10 mM HEPES, pH 7.5, 2 mM CaCl 2 , 150 mM Nmethyl- D-glucamine and Ringers buffer containing 10 mM HEPES, pH 7.5, 150 mM NaCl, 3 mM ... and 20 mM sucrose. 4 parts NMG and 1 part Ringers buffer were prepared containing an additional 2.2 mM ...

Screening of Corticosteroids in Urine by Negative Atmospheric Pressure Chemical Ionization LC/MS/MS

... (internal standard) solution, 1 mL phosphate buffer 0.8M pH 7.0 and 25 μL beta-glucuronidase are ... the glucuronic acid). Adding 750 μL of 20% buffer K 2 CO 3 / KHCO 3 (1:1), which brings the pH ... of each corticosteroid were prepared in a 20% buffer A / 80% methanol mixture. The mixture was meant ...

Quantitative Analysis of the Cell Cycle in Imaging Applications

... No. 552598a) for 20min, washed with BD Perm/Wash buffer (PBS with 0.1% saponin and 3% fetal calf serum, ... again in BD CytoPerm Plus Permeabilization buffer (BD 552598c) for 10 min. Afterwards, they were ... in PBS at 37C for 1 hour), washed in Perm/Wash buffer and stained simultaneously with Alexa Fluor 488 ...

Analysis of Blood Serum on the Liberty Series II ICP-AES with the axially-viewed plasma

... the effect of adding cesium as an ionization buffer to the standards and samples was also investigated. Cesium was chosen as an ionization buffer as it has a low energy of ionization, is not very ... line was used. No internal standard or ionization buffer was added. Triplicate analyses were done and the ...

Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents

... after the cells are placed in the lysis buffer or a tissue sample is homogenized in it. The ... is formulated as a 2X solution. The final lysis buffer concentration in any lysis step should always be ... tip at least three times, mixing the lysis buffer with the cell pellet. 5.Place the cell ...

A Mix-and-Read Cell-Based Assay for Hybridoma Screening Using the FMAT 8100 HTS System

... collected, washed with screening buffer (PBS, 2% FCS), and resuspended in screening buffer at a concentration of 1x105 cells/mL. ... wells were blocked with 200 L of screening buffer for 1 hour. ...

Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

... Cat. # KIT0202). a) 50 l extraction buffer was pipetted into a 0.5 ml microcentrifuge tube. ... inverted the assembly such that the extraction buffer completely covered the cap surface. ... e) Eluted the RNA in 11 l elution buffer following the PicoPure protocol. ...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

... (Sigma cat. #45830) ◊ SSC buffer (Sigma cat. #S8015) ◊ RNase A ... to the time the captured cells are in extraction buffer as brief as possible, preferably under 45 ... captured on an HS LCM cap, 15 L of extraction buffer from the PicoPure RNA isolation kit was added to ...

The development of a serotonin 5-HT2C receptor-binding assay using the LEADseeker Multimodality Imaging System

... 1. Reagents were added in the following order: buffer or DMSO solution, unlabeled ligand (NSB wells), ... tolerance studies, DMSO was diluted in binding buffer to give final concentrations per well as shown in ... Dilutions of DMSO were prepared in assay buffer to give a range of concentrations from 0.002% ...

The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System

... Reagents were added in the following order: assay buffer or buffer containing 2% (v/v) DMSO solution, unlabeled ... in a 20-µl volume, and 10 µl of assay buffer in the absence of competing ligand. For ...

Use of the HitHunter EFC Chemiluminescent Detection Kits with LEADseeker Multimodality Imaging System

... control wells by adding 15 µl assay buffer to appropriate wells.

1.5 Add 5 µl assay buffer to all enzyme standard curve wells.

buffer to appropriate wells.

2.3 Create ...

Performance of fluorescent dyes with LEADseeker Multimodality Imaging System

... 10 mg/ml. Subsequently, each dye was diluted in buffer (PBS containing 0.01% [v/v] Brij™-35) to ... of concentration, each dye was diluted further in buffer to a top working concentration of 6.4 nM. It was then subjected to doubling dilution in buffer to produce a low concentration of 0.00625 nM. ...

Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3', 5' monophosphate and its validation for pharmacological screening at G protein-coupled receptors

... materials required Krebs-Henseleit buffer Phosphate buffered saline ... suspension (2 µl) prepared in physiological buffer such as Krebs-Henseleit buffer, PBS, or ... with assays carried out in PBS or Krebs-Henseleit buffer using a well-established method involving cAMP ...

The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies

... red free (Invitrogen 51200-038), or Krebs-Ringer buffer (see method) Agonists: Isoproterenol, ... available antibodies may be supplied in buffer containing sodium azide. This can affect labeling ... Replace the medium with 50 µl Krebs-Ringer buffer containing 2.5–5 µg/ml CypHer5E ...

The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR

...

Assay medium: MEM, or Krebs-Ringer buffer (see method)

Agonists: ... expressing cells) or 100 µ l Krebs-Ringer buffer (for TRH and angiotensin II receptor expressing ... 100 µ l of assay medium or Krebs-Ringer buffer containing agonist and incubate at 37 °C for ...

The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System

... Reagents were added in the following order: buffer or DMSO solution, unlabeled ligand (NSB wells), ... in a 10-µl volume, and 20 µl of assay buffer in the absence of competing ligand. For ... of competing ligand and 10 µl of assay buffer was added to [ 125 I]CGRP, bead, and receptor, ...

Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system

... (Sigma) with 10% CS-FCS (Sigma)

Assay buffer 47.7-ml media, 500-µl of 1-M HEPES, 500 ... BRL)

Anisomycin, 300 nM, in assay buffer (Sigma)

SB203580-HCl, 100 µM, containing anisomycin (300 nM) in assay buffer (Sigma).

Formalin solution (10%), ...

The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System

... in a 20-µl volume, and 10 µl of assay buffer in the absence of competing ligand. For ... 10 µl of competing ligand prepared in assay buffer was added with [ 125 I] NDP-α-MSH (10 ... DMSO tolerance studies, DMSO was diluted in assay buffer to give final concentrations per well as shown in ...

The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System

... Reagents were added in the following order: assay buffer or buffer containing 2% (v/v) DMSO solution, unlabeled ... in a 20-µl volume, and 10 µl of assay buffer in the absence of competing ligand. For ...
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