Navigation Links
Blotting in Biological Technology

LI-COR Updates Product and Application Guide for Summer 2008

... antibodies, protein labeling kits, Western blotting kits, In-Cell Western(TM) assay kits, Western blotting buffers, and protein stains. The guide ... New products include the MPX blotting System that creates up to 24 independent channels ...

LI-COR(R) Biosciences Introduces New Tools for High Throughput Western Blotting

... introduces a new accessory, MPX(TM) (Multiplex) blotting System, for its Quantitative Western blotting product line. The MPX System can be incorporated ... pathway analysis, or any other common Western blotting procedure that generates a PVDF or nitrocellulose ...

Sigma-Aldrich and Atlas Antibodies Announce 2,300 New, Highly Validated Prestige Antibodies(RM)

... with over 500 tissue immunohistochemistry (IHC) images, over 100 cell/line immunocytochemistry (ICC) images, immunofluorescence (IF) and western blotting (WB) data, all publicly accessible via the Human Protein Atlas ( www.proteinatlas.org ). In August 2008, Sigma-Aldrich added over 2,000 ...

Technical Note: E-PAGE(TM) 48- and 96-Well Gels on the Odyssey(R) Infrared Imaging System

... Corporation, Carlsbad, CA) provide a high-throughput SDS-PAGE method for processing protein samples. When used in conjunction with the iBlot(TM) Dry blotting System, up to 96 different proteins samples can be electrophoresed and transferred to a membrane in as little as 40 minutes. Alternatively, a ...

Norgen Biotek Corp. Launches Four New Kits for the Purification of RNA and DNA from Plants and Fungi

... analysis. The procedure yields sufficient fungal DNA to be used in a number of downstream applications including Real-Time PCR, sequencing, Southern blotting and SNP analysis and the total procedure can be completed less than 60 minutes. "Our new line of kits to isolate RNA and DNA from plants represents ...

Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting

... ECL Plex™ Western blotting Detection System can detect proteins at picogram ... Introduction ECL Plex Western blotting detection system is based on direct fluorescent ... detection using the ECL Plex fluorescent Western blotting system (28-4015-40) (1), available at ...

Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager

... Ettan DIGE Imager • fluorescent Western blotting • multiplex detection • dynamic ... Introduction The ECL Plex Western blotting system is based on the sensitive CyDye™ ... property of the ECL Plex system, enabling blotting of two proteins simultaneously.

Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell

... membrane. A unique feature of semi-dry blotting is the ability to use two different ... of SDS SDS is used in western blotting transfer buffer to aid in the elution ... The opposing effects of methanol and SDS in blotting can be exploited in semi-dry transfer ...

Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C

... Immun-Blot PVDF membrane for western blotting and Sequi-Blot PVDF membrane for protein ... PVDF Membrane The focus with a blotting membrane is on how well it delivers signal while ... blotting, semi-dry blotting, and dot blotting all deliver excellent results. For ...

Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A

... the AfCS Antibody Laboratory is multiplex western blotting with phosphorylation site-specific ... this protein extraction procedure yields western blotting results that are similar to those that ... (AfCS protocol PP00000046). Western blotting Protein samples (612 g) were ...

Northern Analysis: The Basics

... transcripts. The Northern blotting procedure is straightforward ... protection assays. A third limitation of Northern blotting has been the difficulty ... Although established Northern blotting procedures are up and ...

ECL Semi-dry Blotters

... h • Minimal buffer use—just enough to saturate blotting papers and membranes • Long-lasting perforated ... • Semi-dry blotters validated with GE Healthcare ECL Western blotting systems TE 70 and TE 77 ...

Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A

... CA 94547 USA Introduction Western blotting is a traditional technique for detecting phosphorylated ... and total ELISA assays, are compared with western blotting in terms of sensitivity, detection range, and level of ...

DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell

... SD semi-dry electrophoretic transfer cell, allows blotting of DNA/RNA from agarose gels to nylon ... (V/cm 2 ) which facilitates rapid transfers. The blotting frame allows highly efficient ... staining. 1 Northern and Southern blotting The agarose gel support frame will ...

The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids

... Labeling and detection of DNA (Southern blotting or similar applications) If only a ... similar applications) Northern blotting can be performed with either DNA or RNA probes. Usually, RNA probes ...

A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

... analysis is based on standard molecular biology, with a principal advantage being higher throughput and greater precision than traditional filter and blotting techniques. 32 DNA microarray technology provides a global analysis of gene expression at the level of transcription. It combines standard molecular ...

Ettan DIGE Imager

... The system has also been designed to image a wide range of other fluorescent gel applications, and can be used with the new ECL Plex™ Western blotting System.

The imager is controlled from the Ettan DIGE Imager software, and can be set up for a variety of gel formats. ...

New gravity-flow column gives faster and simpler purification of histidine-tagged proteins

... band can be seen from the band at approximately M r 15 000. Some of the smaller proteins may have passed through the membrane due to a prolonged blotting time. N-terminal sequencing (data not shown) of the three bands confirms that the low molecular weight bands are truncated forms of the ...

PFGE Analysis of Chromosomal DNA From a Single Hair Root

... 200 volts for 24 hours with a 50-90 second switch time ramp. Gels were first stained with ethidium bromide (Figure 1A). Southern blotting and hybridization were performed as previously described. 2,3 The autoradiograph of the gel with the hybridized, labeled DNA ...

Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A

... DNA fragments are then liberated from the protein by treatment with proteinase K, and the DNA fragments are identified by Southern blotting or PCR (Kuo and Allis 1999). More recently, studies have established the utility of the ChIP assay in studying histone complex ...

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

... into nitrocellulose (0,45 m nitrocellulose membrane, Hybond-C, Amersham ) by the method of Towbin. 32 The electrophoretic blotting was performed with 25 mM Tris, 192 mM glycine, pH 8.3, at 30 V overnight. Residual binding sites were blocked with 1.0% ...

Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C

... p23-DHFR protein preparation (Figure 2B, lane 9) was electrophoresed and transferred to a 0.45 m nitrocellulose membrane via blotting (Trans-Blot SD semi-dry transfer cell). Following Poinceau Red staining, membranes were washed twice with phosphate buffered ...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

... system, but can be scaled up when larger amounts are required by using the same conditions and a Model 491 Prep Cell. By western blotting of the purified proteins with porcine hyperimmune antiserum to TGEV (S, M, N) in western blot and the ELISA reaction with ...

Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function

... The involvement of Syt IX in insulin secretion was unclear at the time. Western blotting and RT-PCR analysis revealed the presence of both Syt V and Syt IX in rat pancreatic islets and in INS-1E ...

RNAi in Non-Mammalian Cultured Cells

... href="http://www.ambion.com/catalog/ProdGrp.html?fkApp=1&fkSubApp=13&fkProdGrp=39">RNAqueous Kit , Ambion) and analyzed using by Northern blotting (NorthernMax -Gly Kit , Ambion). ...

RNAlater FAQs: Preserving RNA Before Isolation

... . RNA later will denature proteins, so it is only compatible with routine protein analyses such as Western blotting and 2D gel electrophoresis that do not require native protein [

MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers

... patients were analyzed for expression of miR-16, miR-21, miR-143, miR-145, and let-7 using a secondary method of detection (Northern blotting or mir Vana miRNA Detection Kit). The graphs show the relative abundance of each miRNA (ratio of tumor:NAT) from the array ...

Isolate It All: siRNA miRNA Total RNA Native Protein

... mRNA species are analyzed with techniques that are not compatible, for example gel purification or Northern blotting on 15% acrylamide gels for miRNA/siRNA vs. RT-PCR or microarray analysis for mRNA. With the mir Vana PARIS ...

Detecting miRNA & siRNA

... in lung and ovary and was present in spleen, thymus, and testicle at levels that would not have been detectable with standard Northern blotting techniques (data not shown). The relative abundance of miR-16 and miR-22 miRNA in mouse lung was also confirmed by multi-target ...

A Better Way to Isolate RNA AND Protein From the Same Sample

... Each protein fraction can also be used directly for most common applications, including functional assays, immunoprecipitation, Western blotting or two-dimensional gel electrophoresis (Figure 2 below). For experiments such as gel shift assays or enzymatic assays, protein ...

A New Way to Assess siRNA Delivery Efficiency

... level of GAPDH knockdown: the KDalert GAPDH Assay Kit . Many researchers are currently using quantitative RT-PCR or Western blotting to assess siRNA-induced knockdown. While accurate, these techniques can be time-consuming and labor intensive. Another means to ...

RNeasy Protect Mini, Midi, and Maxi Kits

... freezethaw cycles. RNeasy purified RNA from RNAlater stabilized samples is ideal for downstream processes, such as northern blotting and RT-PCR, from samples stored under a wide variety of conditions (see figure "RNA Stability in Tissues: Different Temperatures ...

QIAamp DNA Blood BioRobot MDx Kit

... check of 20 minutes) with no hands-on time during the run. The resulting DNA performs well in downstream amplification and Southern blotting assays (see figures " No PCR Inhibition " and " Reliable Real-Time PCR "). Advantages of the system ...

Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells

... group of products that simplifies Northern blot analysis and leads to quick and reliable results. Methods Isolation, Electrophoresis, and blotting of Total RNA: Total RNA was isolated from 5 x 10 6 CHO cells using the StrataPrep total RNA miniprep kit. 3 Duplicate samples containing 2, 1, ...

Imaging Chemiluminescent Western Blots

... video system can capture light emitted from the wide variety of substrates typically used in chemiluminescent Southern, Northern and Western blotting techniques, we have found that the substrate choice can have an impact on the quality of the resulting images. Chemiluminescent Western Blots: ...

Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements

... gene encodes the firefly luciferase enzyme that can be monitored easily, inexpensively and quantitatively. Reporter enzyme assays replace tedious blotting procedures and are widely used due to their convenience, sensitivity and ability to generate reliable in vivo data. 3 PathDetect ...

Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells

... tag is positioned for C-terminal fusion to the protein of interest. Therefore, the induction of FLAG fusion proteins can be monitored by Western blotting or other immunodetection methods using the a -FLAG M2 antibody (data not shown). Conclusions The Complete Control system has several ...
Other Contents
(Date:11/10/2014)... began appearing on U.S. store shelves in early 2010, ... since. The small packets can be tossed into a ... liquid or powder. The convenience, though, has come with ... researchers at Nationwide Children,s Hospital found that from 2012 ... 17,230 children younger than 6 years of age swallowing, ...
(Date:11/7/2014)... Florida , November 6, 2014 ... authentication for better mobile security revolutionizing online transactions.  Companies in ... Alibaba Holdings Ltd. (NYSE: BABA ), Google Inc. ... MSFT ), eBay Inc. (NASDAQ: EBAY ) and ... NXTD and NXTDW), a biometric authentication company focused ...
(Date:11/6/2014)... an asset for a predator. Except when that predator runs ... beetle, relative to its size, is the fastest creature on ... lengths per second (at about five miles per hour). The ... the sprinting gold from the tiger beetle, a person would ... tiger beetle has a problem. At peak speeds, everything becomes ...
Breaking Biology News(10 mins):Study finds laundry detergent pods, serious poisoning risk for children 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 3Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 4Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 5Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 6The tiger beetle: Too fast to see 2
(Date:11/22/2014)... The topic of B&D Rockeries’ latest ... walls. The article states that, “While they give off the ... that go into creating this powerful look.” , One of ... is choosing which kind of block matches your home environment ... says, “The two main options are concrete and stone blocks. ...
(Date:11/22/2014)... 22, 2014 Forbes Living TV ... pleased to announce that Ashley Lombardo and friend Candy ... , Ms. Lombardo entered friend Candy Sherman to ... wrote of how her friend is courageously battling lung ... single mom. Despite medical bills piling up and the ...
(Date:11/22/2014)... (PRWEB) November 22, 2014 “Greenfield Naturals” ... Tech Report , which features the latest and ... technology expert and reporter for NewsWatch, conducted the product ... improve the flavor of your water. , From pollution ... It’s important that everything is done to remove these ...
(Date:11/22/2014)... News) -- Those air-blown hand dryers in public restrooms ... a new study suggests. British researchers placed a ... in order to simulate poorly washed hands. They then ... paper towels to dry their hands. The investigators ... germs around both types of dryers than around towel ...
(Date:11/21/2014)... Porcelain veneer dentist, Dr. Kevin ... preparation for the coming holiday season. Veneers, ultra-thin shells ... completely natural, and can be used to permanently conceal ... for their ability to strengthen teeth. Veneers are an ... one or several teeth to rejuvenate appearance. Check there ...
Breaking Medicine News(10 mins):Health News:5 Step Installment of Block Retaining Walls is Topic of Latest Article by B&D Rockeries 2Health News:Forbes Living TV Announces Winner of Thanksgiving #PayItForward Contest 2Health News:An Easy Home Solution to Get Better Tasting Water was Featured on NewsWatch Television on October 30, 2014 2Health News:Restroom Hand Dryers Spread More Germs Than Paper Towels, Study Finds 2Health News:Los Angeles Porcelain Veneers Dentist, Dr. Kevin Sands, Now Creates Picture-Perfect Smiles for Patients for the Holiday Season 2
Other TagsOther Tags