LI-COR Updates Product and Application Guide for Summer 2008
... antibodies, protein
labeling kits, Western blotting
kits, In-Cell Western(TM) assay kits,
buffers, and protein stains. The guide ...
New products include the MPX blotting
System that creates up to 24
independent channels ...
LI-COR(R) Biosciences Introduces New Tools for High Throughput Western Blotting
... introduces a
new accessory, MPX(TM) (Multiplex) blotting
System, for its Quantitative
product line. The MPX System can be incorporated ... pathway analysis, or any
other common Western blotting
procedure that generates a PVDF or
Sigma-Aldrich and Atlas Antibodies Announce 2,300 New, Highly Validated Prestige Antibodies(RM)
... with over 500 tissue immunohistochemistry (IHC) images, over 100 cell/line immunocytochemistry (ICC) images, immunofluorescence (IF) and western blotting
(WB) data, all publicly accessible via the Human Protein Atlas ( www.proteinatlas.org ).
In August 2008, Sigma-Aldrich added over 2,000 ...
Technical Note: E-PAGE(TM) 48- and 96-Well Gels on the Odyssey(R) Infrared Imaging System
... Corporation, Carlsbad, CA) provide a high-throughput SDS-PAGE
method for processing protein samples. When used in conjunction with the
iBlot(TM) Dry blotting
System, up to 96 different proteins samples can be
electrophoresed and transferred to a membrane in as little as 40 minutes.
Alternatively, a ...
Norgen Biotek Corp. Launches Four New Kits for the Purification of RNA and DNA from Plants and Fungi
... analysis. The procedure yields sufficient fungal DNA to be
used in a number of downstream applications including Real-Time PCR,
sequencing, Southern blotting
and SNP analysis and the total procedure can
be completed less than 60 minutes.
"Our new line of kits to isolate RNA and DNA from plants represents ...
Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
ECL Plex™ Western blotting
Detection System can detect proteins at picogram ... Introduction
ECL Plex Western blotting
detection system is based on direct fluorescent ... detection using the ECL Plex fluorescent Western blotting
system (28-4015-40) (1), available at ...
Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
... Ettan DIGE Imager • fluorescent Western blotting
• multiplex detection • dynamic ... Introduction
The ECL Plex Western blotting
system is based on the sensitive CyDye™ ... property of the ECL Plex system, enabling blotting
of two proteins simultaneously.
Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell
A unique feature of semi-dry blotting
is the ability to use two different
... of SDS
SDS is used in western blotting
transfer buffer to aid in the elution
... The opposing effects of methanol and SDS in blotting
can be exploited
in semi-dry transfer ...
Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C
... Immun-Blot PVDF membrane for western
and Sequi-Blot PVDF membrane for protein ... PVDF Membrane
The focus with a blotting
membrane is on how well it delivers signal while ...
blotting, semi-dry blotting, and dot blotting
all deliver excellent results.
Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
... the AfCS Antibody Laboratory is multiplex western blotting
with phosphorylation site-specific ... this protein extraction procedure yields western blotting
that are similar to those that ... (AfCS protocol PP00000046).
Protein samples (612 g) were ...
Northern Analysis: The Basics
The Northern blotting
straightforward ... protection assays. A third limitation of Northern blotting
been the difficulty ... Although established Northern blotting
procedures are up and ...
ECL Semi-dry Blotters
• Minimal buffer use—just enough to saturate blotting
papers and membranes
• Long-lasting perforated ... • Semi-dry blotters validated with GE Healthcare ECL Western blotting
TE 70 and TE 77
Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A
... CA 94547 USA
is a traditional technique for detecting phosphorylated
total ELISA assays, are compared with western blotting
in terms of sensitivity,
detection range, and level of ...
DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell
... SD semi-dry electrophoretic transfer cell, allows blotting
from agarose gels to nylon ... (V/cm 2 ) which facilitates rapid transfers. The blotting
frame allows highly efficient ... staining. 1
Northern and Southern blotting
The agarose gel support frame will ...
The DIG System Nonradioactive and
Highly Sensitive Detection of Nucleic Acids
Labeling and detection of DNA
or similar applications)
If only a ... similar applications)
can be performed with
either DNA or RNA probes. Usually, RNA
A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology
... analysis is based on standard molecular biology, with a principal advantage being higher throughput and greater precision than traditional filter and blotting
techniques. 32 DNA microarray technology provides a global analysis of gene expression at the level of transcription. It combines standard molecular ...
Ettan DIGE Imager
... The system has also been designed to image a wide range of other fluorescent gel applications, and can be used with the new ECL Plex™ Western blotting
The imager is controlled from the Ettan DIGE Imager software, and can be set up for a variety of gel formats. ...
New gravity-flow column gives faster and simpler purification of histidine-tagged proteins
... band can be seen from the band at approximately M r 15 000. Some of the smaller proteins may have passed through the membrane due to a prolonged blotting
time. N-terminal sequencing (data not shown) of the three bands confirms that the low molecular weight bands are truncated forms of the ...
PFGE Analysis of Chromosomal DNA From a Single Hair Root
... 200 volts for 24 hours with
a 50-90 second switch time ramp.
Gels were first stained with ethidium bromide (Figure 1A). Southern blotting
and hybridization were performed as previously described. 2,3 The autoradiograph
of the gel with the hybridized, labeled DNA ...
Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
... DNA fragments are then liberated from the protein by treatment with proteinase
K, and the DNA fragments are identified by Southern blotting
or PCR (Kuo
and Allis 1999). More recently, studies have established the utility of
the ChIP assay in studying histone complex ...
Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing
... into nitrocellulose (0,45 m
nitrocellulose membrane, Hybond-C, Amersham ) by the method of Towbin. 32
The electrophoretic blotting
was performed with 25 mM Tris, 192 mM glycine,
pH 8.3, at 30 V overnight. Residual binding sites were blocked with 1.0%
Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C
... p23-DHFR protein preparation (Figure
2B, lane 9) was electrophoresed and transferred to a 0.45 m nitrocellulose
membrane via blotting
(Trans-Blot SD semi-dry transfer cell). Following
Poinceau Red staining, membranes were washed twice with phosphate buffered
Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A
... system, but can be scaled up when larger amounts are required
by using the same conditions and a Model 491 Prep Cell.
By western blotting
of the purified proteins with porcine
hyperimmune antiserum to TGEV (S, M, N) in western blot
and the ELISA reaction with ...
Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function
involvement of Syt IX in insulin secretion was unclear at the
and RT-PCR analysis
revealed the presence of both Syt V and Syt IX in rat
pancreatic islets and in INS-1E ...
RNAi in Non-Mammalian Cultured Cells
... href="http://www.ambion.com/catalog/ProdGrp.html?fkApp=1&fkSubApp=13&fkProdGrp=39">RNAqueous Kit , Ambion) and analyzed using by Northern blotting
(NorthernMax -Gly Kit , Ambion). ...
RNAlater FAQs: Preserving RNA Before Isolation... . RNA later will denature proteins, so
it is only compatible with routine protein analyses such as
Western blotting and 2D gel electrophoresis that do not
require native protein [
MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers
... patients were analyzed for expression of miR-16, miR-21, miR-143, miR-145,
and let-7 using a secondary method of detection (Northern blotting
mir Vana miRNA Detection Kit). The graphs show the relative
abundance of each miRNA (ratio of tumor:NAT) from the array ...
Isolate It All: siRNA miRNA
Total RNA Native Protein
... mRNA species are analyzed with
techniques that are not compatible, for example gel
purification or Northern blotting
on 15% acrylamide gels for
miRNA/siRNA vs. RT-PCR or microarray analysis for mRNA. With
the mir Vana PARIS ...
Detecting miRNA & siRNA
... in lung and ovary and was present in spleen, thymus, and testicle at levels
that would not have been detectable with standard Northern blotting
(data not shown). The relative abundance of miR-16 and miR-22 miRNA in
mouse lung was also confirmed by multi-target ...
A Better Way to Isolate RNA AND Protein From the Same Sample
... Each protein fraction can also be used directly for most common applications,
including functional assays, immunoprecipitation, Western blotting
two-dimensional gel electrophoresis (Figure 2 below). For experiments
such as gel shift assays or enzymatic assays, protein ...
A New Way to Assess siRNA Delivery Efficiency
... level of GAPDH knockdown: the KDalert
GAPDH Assay Kit .
Many researchers are currently using quantitative RT-PCR or Western blotting
to assess siRNA-induced knockdown. While accurate, these techniques can
be time-consuming and labor intensive. Another means to ...
RNeasy Protect Mini, Midi, and Maxi Kits
... freezethaw cycles. RNeasy purified RNA from
RNAlater stabilized samples is ideal for downstream processes, such as
and RT-PCR, from samples stored under a wide variety
of conditions (see figure "RNA Stability in Tissues: Different Temperatures
QIAamp DNA Blood BioRobot MDx Kit
... check of 20 minutes) with no hands-on time during the run. The resulting
DNA performs well in downstream amplification and Southern blotting
assays (see figures " No PCR Inhibition "
and " Reliable Real-Time PCR "). Advantages
of the system ...
Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
... group of products that simplifies
Northern blot analysis and leads to quick and reliable results.
Isolation, Electrophoresis, and blotting
of Total RNA: Total RNA was
isolated from 5 x 10 6 CHO cells using the StrataPrep total RNA
miniprep kit. 3 Duplicate samples containing 2, 1, ...
Imaging Chemiluminescent Western Blots
... video system can capture light emitted from the
wide variety of substrates typically used in chemiluminescent Southern, Northern
and Western blotting
techniques, we have found that the substrate choice can
have an impact on the quality of the resulting images.
Chemiluminescent Western Blots: ...
Signal Transduction Reporting Systems Using Cis-Acting Enhancer
... gene encodes the
firefly luciferase enzyme that can be monitored easily, inexpensively and
quantitatively. Reporter enzyme assays replace tedious blotting
are widely used due to their convenience, sensitivity and ability to generate
reliable in vivo data. 3
Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in
... tag is positioned for C-terminal fusion to
the protein of interest. Therefore, the induction of FLAG fusion proteins can be
monitored by Western blotting
or other immunodetection methods using the a -FLAG
M2 antibody (data not shown).
The Complete Control system has several ...