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Blot in Biological Technology

LI-COR Blot Washer Automates Western Blot Processing

... -- LI-COR Biosciences now offers the LI-COR blot Washer for automating the tedious process of blot washing and secondary antibody addition for ... wash cycles for one or multiple blots, the LI-COR blot Washer is an easy to use liquid delivery and ...

LI-COR Updates Product and Application Guide for Summer 2008

... MPX Blotting System that creates up to 24 independent channels on a single blot for chemiluminescence, colorimetric, and infrared imaging applications. ... all common Western procedures that generate a PVDF or Nitrocellulose blot of 7 x 8.5 cm to the MPX format. The new Pearl(TM) In Vivo Small Animal ...

LI-COR(R) Biosciences Introduces New Tools for High Throughput Western Blotting

... common Western blotting procedure that generates a PVDF or nitrocellulose blot of 7 x 8.5 cm. Processing is as simple as clamping the blot into the MPX System, creating up to 24 independent channels. Channel ports ...

LI-COR Introduces the Odyssey(R) Two-Color NIR Fluorescent Protein Molecular Weight Marker

... polyacrylamide gels and can be used to visualize the progress of protein separation during electrophoresis, to monitor transfer efficiency to Western blot membrane, and to determine the molecular weight of proteins of interest. The marker contains 10 proteins ranging in size from 10-250 kDa. It is ...

LI-COR August 27th On-Line Webinar Introduces New Approach to Small Animal In Vivo Imaging

... the upcoming webinar visit http://licorevents.webex.com and click on the 'Register' link. Past webinars including Quantitative, Multiplexed Western blot Detection with Infrared Fluorescence are available for viewing at http://www.licor.com/bio/video/index.jsp . About LI-COR Biosciences LI-COR(R) ...

Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager

... where two proteins can be detected in the same blot with minimal cross-reactivity between antibodies ... both single-protein and multiplex analyses. The blot scans were evaluated, resulting in data that was ... IgG-Cy5 are displayed below the blot images in Figure 1. A sensitivity of 0.6 pg was ...

Transgenic Zebrafish by Electroporation

... used for a dot blot. DNA for the dot blot is alkaline denatured, vacuum blotted ... to some extent (based on the dot blot analysis). Of these positive animals, ... than the 60 pg control). A typical dot blot is shown in Figure 2. ...

Transformation of Filamentous Fungi by Microprojectile Bombardment

... of transformants, and Southern blot hybridization patterns were compared between ... al ., 1996. DNA isolations and Southern blot analysis DNA was isolated from ... at a single site (Waring et al ., 1989). Southern blot hybridizations were carried out as ...

Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A

... to prepare cell lysates for western blot and Bio-Plex analysis (refer to bulletin 3033). A ... in bulletin 3033 was followed for western blot analysis. The procedure provided in ... each target were used in both western blot and Bio-Plex phosphoprotein and total target ...

DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell

... any gel pretreatments. The Trans- blot SD semi-dry electrophoretic transfer cell ... buffer for transfer. The extra thick blot paper must be identical to the dimensions of the agarose gel. Bio-Rads extra thick blot paper is available in four sizes: 7.5 ...

Northern Analysis: The Basics

... than 100,000 molecules on a blot with ULTRAhyb, making the decision to use RNA or ... to produce biotinylated probes for blot hybridizations. For more information ... method that increases blot sensitivity by efficiently moving RNA, ...

RNAi: Size Does Matter

... For both targeted genes, Western blot analyses showed a stronger silencing effect with ... used in this study. (B) Western blot analysis 72 hours post treatment with 10 nM of ... promoters on both ends. (D) Western blot analysis 72 hr post treatment with ...

RNAi in Non-Mammalian Cultured Cells

... treated with 10 nM of dsRNA. Western blot analysis 72 hours after treatment ... expression was demonstrated by Northern blot analysis (Figure 2A and 2B). This ... levels. The RNAi effect was analyzed by Western blot with anti-U2AF 50 (50 kDa) and anti-hrp48 (48 ...

Isolate It All: siRNA miRNA Total RNA Native Protein

... of the same samples by Northern blot confirmed that smaller RNA species such as the ... miR-16 miRNA was detected by Northern blot with an antisense RNA probe 5' labeled and ... the indicated RNA species by Northern blot or by solution hybridization assay ...

New Tools for Small RNA Analysis

... of miR-124, previously determined by Northern blot analysis using 30 g of total RNA (3). ... hybridization assays, but also for Northern blot or in situ hybridization ... RNA structure/function studies, Northern blot and primer extension analysis. For ...

Efficient Cellular Fractionation for RNA and Protein Isolation

... (NorthernMax-Gly Kit) or Western blot with anti-GAPDH antibody (Ambion). ... protein level was confirmed by Western blot analysis with an antibody specific for the ... previously determined by Western blot analysis in Figure 1 and RT-PCR in ...

Choose the Right Transfection Agent for Your RNAi Experiment

... analyzed at the mRNA level by Northern blot 72 hours after transfection. In all three cell ... and GAPDH mRNA levels were analyzed by Northern blot using Ambion's blot 72 hours after transfection. RNA was ...

A Better Way to Isolate RNA AND Protein From the Same Sample

... of downstream applications, including blot hybridization, in vitro translation, cDNA ... native functional protein, such as Western blot analysis. ... the PARIS Kit and analyzed by Northern blot and Western blot. ...

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

... fusion protein, we performed Western blot analyses with anti-c-myc antibody using cell ... was being detected, we also performed Western blot analyses with an antiluciferase ... fusion protein is easily detected in both Western blot analyses ( Figure 3 ). ...

Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells

... system simplifies and improves Northern blot analysis Karen Dolter Jeff Braman ... When used together for Northern blot analysis, this excellent integrated system ... and reproducible results. Northern blot analysis of gene transcription is dependent on ...

Imaging Chemiluminescent Western Blots

... to capture an image following Western blot analysis. Dilutions of Stratagenes purified ... nitrocellulose membranes and subjected to Western blot analysis using Stratagenes PY20 Monoclonal ... for helpful discussions concerning Western blot analysis. REFERENCES ...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

... Tag by Thrombin Digestion and Anti-FLAG Western blot of FLAG-JNK The CBP affinity purification ... by anti-FLAG M2 antibody, western blot analysis was performed on the thrombin digest ... filter and subjected to western blot analysis with an anti-c-Jun antibody ...

Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter

... reactions. Nucleotides labeled in this way can be used as probes in dot blot assays. The phosphorylation of proteins by kinases is an important ... applications are easily done in a microplate or a microplate format dot blot apparatus. The sample is measured directly in its own medium with little ...

Electroporation of Primary Bone Marrow Cells

... DNA was isolated from the bone marrow cells for Southern blot analysis. 2,3 As shown in Table 1, a 3.5-fold increase was seen ... and a pulse time constant of 10 msec was achieved. Southern blot analysis was done to verify the presence of the dhfr gene ...

Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A

... 1B, lane 5) and its virus specificity was confirmed by western blot (Figure 1C, lane 5). After concentration of fractions 913, the ... the full size recombinant TGEV-M protein was detected by western blot (Figure 1C, lanes 3 and 5). The results from this study suggest ...

Superior Gene Silencing Using Adenoviral Vectors

... sections were analyzed by fluorescence microscopy and Western blot (Figure 2). GFP expression was reduced in the injected brain ... (il) and contralateral (cl) portions and analyzed by Western blot using antibodies to GFP and -actin (control). ...

Stabilizing RNA in Fresh and Frozen Tissues for RNA Isolation

... at -20C. Both the stained gel and the resulting Northern blot demonstrate that RNA isolated from tissues treated with RNA ... a denaturing agarose gel. (B) shows the results of Northern blot analysis of the same gel hybridized to radiolabeled probes for ...

RNAi: Get the Whole Story

... using the PARIS Kit. mRNA knockdown was evaluated by Northern blot using an antisense radiolabeled RNA probe transcribed from pTRI-GAPDH ... and protein for either real-time RT-PCR (Panel A) or Western blot analysis (Panel B). RNA was reverse transcribed using the ...

Getting Started with MicroRNA Research

... be confirmed by a secondary method, such as Northern blot analysis or the more sensitive solution hybridization ... mirVana miRNA Detection Kit ). Probes for Northern blot or solution hybridization analysis can be generated by ...

MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers

... mA in 0.5X TBE for 2 hr. After drying, the Northern blot was incubated overnight in 10 ml ULTRAhyb-Oligo ... with the mir Vana Probe & Marker Kit (Ambion). The blot was washed 3 x 10 min at room temperature in 2X SSC, ...

Isolate Total RNA and Protein From the Same Sample

... was resolved on a 10% SDS polyacrylamide gel and analyzed by Western blot with an anti-GAPDH antibody (Ambion) (left bottom). Real time ... on a 15% denaturing polyacrylamide gel and analyzed by Northern blot with a 5S rRNA probe 5' labeled and purified with the mir Vana ...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

... total RNA was analyzed on a denaturing agarose gel. A Northern blot was used to detect let-7 miRNA, 5.8S and 5S rRNA while miR-16 ... pre-miRNA hairpins as determined by Northern blot analysis (data not shown). High Level of Protein ...

Get the miRNA OutPlant Applications

... but instead are analyzed typically by Northern blot using 15% acrylamide gels or specialized solution ... fraction depleted of small RNAs. Further analysis by Northern blot showed that the same amount of microRNAs miR-163 and miR-167 ...

Detecting miRNA & siRNA

... thymus. These variations across tissues were confirmed by Northern blot analysis with the same mir-16 probe. Interestingly the mir-22 ... expression of miR-16 across tissues was observed by Northern blot analysis (2 days exposure) or by hybridization in solution (2 ...

Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer

... Figure 3. Western blot of P2X3 expressed in HEK cells. ... monitor the effectiveness of ICAT derivatization of P2X3, Western blot analysis was done using sample preparations before and after ICAT ...

RNeasy Protect Mini, Midi, and Maxi Kits

... electrophoresis and expression of GAPDH was examined using northern blot analysis. M: markers. RNA ... RNA was analyzed on a formaldehyde agarose gel and northern blot (GAPDH-probed). Stabilized mouse liver tissue was subjected to ...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

... dependant on the annealing temperature (Fig. 2a). A PCR blot analysis using an isoamylase-specific oligonucleotide probe was ... Fig. 2 B . DNA blot analysis of PCR products from Fig. 2a with an ...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

... dependant on the annealing temperature (Fig. 2a). A PCR blot analysis using an isoamylase-specific oligonucleotide probe was ... Fig. 2 B. DNA blot analysis of PCR products from Fig. 2a with an ...

Enhancing PCR* Efficiency with MasterTaq

... labeled with ... dATP for use as probes in a Northern blot experiment (Fig. 2). Using this strategy, it was possible to ... Figure 2: Northern blot of LT23 human lung adenocarcinoma cell RNA (8 g) hybridized ...
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