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Anneal in Biological Technology

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

... gene of L. monocytogenes, Listeria innocua, and Listeria seeligeri by the polymerase chain reaction (PCR)* using primers that anneal to conserved sequences, yielded multiple copies of gene fragments that, when evaluated by density gradient gel electrophoresis ...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

... II reverse transcriptase (Invitrogen) according to the manufacturers instructions. Gene-specific primers (GSPs) were designed to anneal near the 3' end of two mRNA transcripts and to generate PCR products 75300 base pairs long. Three GSP pairs amplify different ...

RNA Amplification for Array Analysis

... the typical probe orientation for microarrays? Oligonucleotides are single-stranded and therefore only anneal to their complement. Probes for microarrays are either generated from an RT reaction (cDNA) or from linear ...

Thermal Cycling Profile for Standard PCR

... heating of the PCR mixture for 2 minutes at 9495C is enough to completely denature complex genomic DNA so that the primers can anneal to the template as the reaction mix is cooled. If the template DNA is only partially denatured, it will tend to snap-back very ...

Primer design

... the required primers (see figure below). Make primers that anneal to sequences in exons on both sides of an intron (Panel 1). With these primers, any product amplified from genomic DNA will be ...

QIAGEN Multiplex PCR Handbook

... of the primer mix. 9. Amplification of pseudogene sequences Primers may anneal to pseudogene sequences and additional PCR products may be amplified. Review primer design to avoid detection of ...

Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates

... renaturation of the template and the formation of secondary structures within each strand before the primers have a chance to anneal to their specific template sites. These properties of GC-rich DNA sequences lead to inefficient primer-template annealing and ...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

... that are complementary to the single-stranded tails of the pCAL-n-EK vector. The vector and insert DNA are combined, allowed to anneal at room temperature and transformed into highly competent bacterial host cells. The resultant colonies can then be screened for ...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

... is purified, then treated with Pfu DNA polymerase in the presence of dATP. The vector and insert DNA are combined, allowed to anneal at room temperature, and transformed into highly competent bacterial host cells. The resultant colonies can then be screened ...

Map and Link Human Genetic Disorders with SSLP Analysis

... location. 1 Often these markers are (CA)n repeat polymorphisms. 1 These polymorphisms can easily be genotyped by PCR with primers that anneal to single-copy DNA, which flanks the repetitive element. Genotyping, using microsatellite markers, at specific locations throughout the ...

Performance Comparisons of Commercial RT-PCR Systems

... and RNA (200 ng of total RNA). The reaction mixtures were incubated at 65C for 5 minutes and cooled to room temperature to allow the primers to anneal to the RNA. MMLV-RT (10 U) was added, and cDNA synthesis was carried out at 37C for 15 minutes (0.3- and 1.8-kb targets) or 30 minutes (7.6-kb ...

prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification

... ng of bacteriophage MS2 RNA). The reaction mixtures were incubated at 65C for 5 minutes, then cooled to room temperature to allow the primers to anneal to the RNA. A total of 10 U of MMLV-RT was added, and cDNA synthesis was carried out at 37C for 15 minutes (targets <2 kb) or 30 minutes (targets ...

Pfu DNA Polymerase as a Hot Start Alternative

... polymerase at lower temperatures ( figure 2 ), Taq DNA polymerase has a greater potential for extending mispaired primers that anneal during these three low-temperature phases of PCR amplification. Hot start PCR protocols have been developed to reduce or prevent ...
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(Date:4/24/2014)... that the world,s oceans play a crucial role ... between Earth,s ecosystems and atmosphere. Now scientists at ... have taken a leap forward in understanding the ... use carbon dioxide to make new cells, a ... into the sea as a buffet of edible ...
(Date:4/24/2014)... NY, April 24, 2014GEN Publishing recently introduced ... publication focusing on the application of OMICs technologies ... next-gen sequencing, are beginning to transform medical care ... over the past decade-and-a-half. , "GEN,s editors and ... pharmacogenomics, genomics, metabolomics, transcriptomics, etc. etc. for years," ...
(Date:4/24/2014)... Scientific Reports journal. , CSIC researcher Carles Lalueza-Fox, ... CSIC and Pompeu Fabra University-UPF), explains: "When the Y chromosome ... it did not match with the DNA recovered from the ... and to make a functional interpretation in order to see ... , The functional genome analysis was based on two main ...
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(Date:4/24/2014)... than one out of three survivors of critical ... The Lancet Respiratory Medicine , and the ... than mentally. , It is one of the ... functional outcomes of critical care survivors, according to ... Medicine, and it highlights a significant public health ...
(Date:4/24/2014)... researcher has found a relationship between motor skill deficiencies ... disorder in very young children. , The findings, ... direct relationship between motor skills and autism severity, indicate ... be included in treatment plans for young children with ... College of Public Health and Human Sciences. , ...
(Date:4/23/2014)... investigators may lead to greater availability and acceptability of ... use of fecal material from healthy donors to treat ... ( C. difficile ) bacteria. In their paper ... Diseases , the researchers report that use of prescreened ... as successful in curing recurrent C. difficile ...
(Date:4/23/2014)... Washington State University Spokane have developed a new ... nod off behind the wheel. , Their recently ... are more variable in drowsy driversand offers an ... video-based driver drowsiness detection systems. , Van Dongen"Video-based ... car is drifting out of its lane are ...
(Date:4/23/2014)... report that rats exposed to high-energy particles, simulating conditions ... show lapses in attention and slower reaction times, even ... ranges. , The cognitive impairments which affected a ... appear to be linked to protein changes in ... to hold true in humans, suggest it may be ...
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