R-Tech Ueno Notifies R&D Items
... (VAP-1) is also called semicarbazide-sensitive amine
oxidase (SSAO). There are two types of ... in inflammation while the latter detoxifies amine
in the living body by its amine
oxidase activity. In other words, VAP-1 is a ...
Paper electrified by copper particles
... reported in an international journal.
The purpose of the research was to test the ability of polymeric and small-molecule compounds that contain amine
groups to protect copper nanoparticles during their manufacturing stage. The particles were manufactured with either poly(ethylene imine) (PEI) or ...
Novel MicroRNA Array Technology for Sensitive miRNA Profiling
... unit specialized for small RNA purification). amine
NTPs are incorporated during tailing ... miRNA are
then labeled with amine
reactive dyes. After hybridization with probes ... is compatible with
reactive dye, including Cy3 and Cy5
TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
Basic and Acidic Compounds:
The tricyclic antidepressant desipramine, containing a secondary amine
group, was analyzed at pH 7.0 to illustrate column performance under conditions
where accessible residual silanol groups would ...
Analysis of a Verapamil Microsomal Incubation using Metabolite ID and Mass FrontierTM
... quick and easy access to MS and MS 2 spectra. This metabolite is likely the result of a three step process where alkyl cleavage at the tertiary amine
group of verapamil initially produces a previously documented metabolic product at mass 290 which then undergoes demethylation to produce a free ...
Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
... H 1 receptor • GPCR • receptor binding assay • LEADseeker • SPA Imaging Beads
Histamine is a biogenic amine
that plays important pathophysiological roles in central and peripheral tissues. Generally released from stimulated mast cells, it contributes to ...
Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
for bead conjugation, as well as to determine the optimum plasma concentration.
The protocol provided with the Bio-Plex amine
coupling kit suggested using
512 g of protein for conjugation. We conjugated beads with 2, 5, 8,
10, and 12 g of virus ...
Which Water to Use?
... and only eliminates RNases present
at the time of treatment. Some solutions (e.g., primary
containing compounds such as Tris) cannot be
treated with DEPC at all.
Optimizing siRNA Transfection for RNAi
... GAPDH or Negative Control #1 siRNA using siPORT amine
Transfection Agent (4 l per well, ...
Transfection Agents can be used for reverse
... cell types.
is manufactured for Ambion by
Optimizing Chemical Transfection and Electroporation of siRNAs
Transfection Agent . Cells were typically
For each experiment, siPORT amine
was added to
pre-plated ... siRNA using 4 l of siPORT
Agent. After 48 hr mRNA expression was evaluated ...
Efficient Delivery of siRNAs to Human
Primary Cells: Electroporation
vs. Chemical Transfection
transfection reagent (Ambion) elicited a decrease
in GAPDH ... Biosciences.
is manufactured for Ambion by Mirus.
TaqMan is a ...
Delivering siRNA Using Adenoviral Vectors
... critical step in
most RNA interference (RNAi) based gene silencing experiments.
While traditional lipid and amine
based reagents work well for
transfecting some cultured cells, they can be inefficient for
Decontamination and inhibition of ribonucleases
... solution, stirring for 1 hour, and autoclaving for one hour to hydrolyze
any remaining DEPC into CO2 and Ethanol. Compounds with primary amine
groups (e.g. Tris) will react with DEPC. Consequently, Tris buffers should
be prepared by dissolving Tris base (from fresh ...
Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer
... marrow (1.7 x 10 -5
versus 1.3 x 10 -5 in controls).
MeIQ (2-amino-3,4-dimethylimidazo[4,5-f ]quinoline) is a potent
found in the human diet. MeIQ induces tumors in several rat
tissues including colon, skin, mammary gland, oral cavity; in the mouse it ...