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Amine in Biological Technology

R-Tech Ueno Notifies R&D Items

... (VAP-1) is also called semicarbazide-sensitive amine oxidase (SSAO). There are two types of ... in inflammation while the latter detoxifies amine in the living body by its amine oxidase activity. In other words, VAP-1 is a ...

Paper electrified by copper particles

... reported in an international journal. The purpose of the research was to test the ability of polymeric and small-molecule compounds that contain amine groups to protect copper nanoparticles during their manufacturing stage. The particles were manufactured with either poly(ethylene imine) (PEI) or ...

Novel MicroRNA Array Technology for Sensitive miRNA Profiling

... unit specialized for small RNA purification). amine NTPs are incorporated during tailing ... miRNA are then labeled with amine reactive dyes. After hybridization with probes ... is compatible with any amine reactive dye, including Cy3 and Cy5 ...

TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds

... Results Basic and Acidic Compounds: The tricyclic antidepressant desipramine, containing a secondary amine group, was analyzed at pH 7.0 to illustrate column performance under conditions where accessible residual silanol groups would ...

Analysis of a Verapamil Microsomal Incubation using Metabolite ID and Mass FrontierTM

... quick and easy access to MS and MS 2 spectra. This metabolite is likely the result of a three step process where alkyl cleavage at the tertiary amine group of verapamil initially produces a previously documented metabolic product at mass 290 which then undergoes demethylation to produce a free ...

Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System

... H 1 receptor • GPCR • receptor binding assay • LEADseeker • SPA Imaging Beads Histamine is a biogenic amine that plays important pathophysiological roles in central and peripheral tissues. Generally released from stimulated mast cells, it contributes to ...

Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System

... virus for bead conjugation, as well as to determine the optimum plasma concentration. The protocol provided with the Bio-Plex amine coupling kit suggested using 512 g of protein for conjugation. We conjugated beads with 2, 5, 8, 10, and 12 g of virus ...

Which Water to Use?

... and only eliminates RNases present at the time of treatment. Some solutions (e.g., primary amine containing compounds such as Tris) cannot be treated with DEPC at all. The

Optimizing siRNA Transfection for RNAi

... GAPDH or Negative Control #1 siRNA using siPORT amine Transfection Agent (4 l per well, ... amine Transfection Agents can be used for reverse ... cell types. siPORT amine is manufactured for Ambion by ...

Optimizing Chemical Transfection and Electroporation of siRNAs

... amine Transfection Agent . Cells were typically ... For each experiment, siPORT amine was added to pre-plated ... siRNA using 4 l of siPORT amine Agent. After 48 hr mRNA expression was evaluated ...

Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection

... amine transfection reagent (Ambion) elicited a decrease in GAPDH ... Biosciences. siPORT amine is manufactured for Ambion by Mirus. TaqMan is a ...

Delivering siRNA Using Adenoviral Vectors

... critical step in most RNA interference (RNAi) based gene silencing experiments. While traditional lipid and amine based reagents work well for transfecting some cultured cells, they can be inefficient for transfecting ...

Decontamination and inhibition of ribonucleases

... solution, stirring for 1 hour, and autoclaving for one hour to hydrolyze any remaining DEPC into CO2 and Ethanol. Compounds with primary amine groups (e.g. Tris) will react with DEPC. Consequently, Tris buffers should be prepared by dissolving Tris base (from fresh ...

Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer

... marrow (1.7 x 10 -5 versus 1.3 x 10 -5 in controls). MeIQ MeIQ (2-amino-3,4-dimethylimidazo[4,5-f ]quinoline) is a potent heterocyclic amine found in the human diet. MeIQ induces tumors in several rat tissues including colon, skin, mammary gland, oral cavity; in the mouse it ...
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