Transgenomic Completes Licensing Option With Dana-Farber Cancer Institute on Cold-PCR for Enrichment of DNA Mutations
... and reliable tool in analyzing DNA variations, especially where the mutant alleles
are at a very low concentration within the sample (less than 1% of the ... Cold-PCR protocols preferentially amplify heteroduplexes such that mutant alleles
become enriched compared to normal alleles. The range of enrichment ...
Honey bee invaders exploit the genetic resources of their predecessors
... from throughout the Africanized honey bee genome showed that most of the alleles
were common to African honey bees. But of the alleles
common to European bees, those found in functional parts of the genome (in ...
ProImmune Launches New MHC-Peptide Binding Assay for Class II HLA Alleles
... available from the Company. The Class II REVEAL(TM) binding assay for HLA alleles
DR1-4, is a
powerful cell-free in vitro assay that can rapidly predict ... extension
of its range of MHC Class II Ultimer(TM) staining reagents with alleles
and DR3 to cover HLA DR1 to 4. Class II Ultimers(TM) can be used ...
New Study Raises Questions About Prostate Cancer Therapies Targeting Insulin-Like Growth Factor Receptor
... IGF1 signaling.
To test their reasoning they conducted a second experiment by crossing
mice carrying the prostate-specific IGF-1R knockout alleles
mice that develop spontaneous prostate cancer when p53 and select other
genes are compromised. The results were as predicted: ...
SNPs Powerful Tools for Association Studies
... to 28 human samples) that revealed seven alleles
at frequencies of 48%, 25%, 12%, 5%, 3%, 3%, and ... bi-allelic (i.e., there are only two possible alleles
at the locus). Because people have two copies of ... although this is extremely rare for minor alleles
that occur at low frequencies. In the Applera ...
Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
(DGGE). When separation of HLA-DRB alleles
is followed by direct sequencing,
this ... rapid way
of identifying all HLA-DRB alleles
for high-resolution tissue typing.
... had been typed previously for HLA-DRB
using both PCR-SSCP and cloning and sequencing ...
Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
... network of control
alleles, 22 of these alleles
can be rapidly and accurately
resolved ... the amplified
chinook salmon A1 chinook alleles
(5, 9, and 10), differing
from one ... curves indicated that all three A1
melted at different concentrations of denaturant ...
Superior Gene Silencing Using Adenoviral Vectors
... Reduce Expression from
To demonstrate that ...
type 3. In 70% of diseased carriers, alleles
contain a G to C mutation.
or mutant polyglutamine (polyQ) alleles
(Atx-Q166-GFP) containing a CAG
Microfluidic analysis of multiplex
PCR products for the genotyping of
... all mPCR reactions including
all involved alleles
separation of seven different ... with
possible disease outcome are the
of the vacA gene,
the presence of the cagA gene ... the combined analysis of the
five different alleles
(vacA s1 or
s2, cagA, vacA m1 or m2) ...
QIAGEN Multiplex PCR Handbook
90 seconds to 3 minutes. However, some alleles
may not be detectable when
using low ... are often highly polymorphic, with many different alleles
a population. alleles
may also differ in the number of copies of a ...
Map and Link Human Genetic Disorders with SSLP Analysis
Quickly identify closely related alleles
with the CastAway
Michelle L. ... polymorphisms), or microsatellites.
that contain these polymorphisms can vary by just ... 2-bp differences between
the upper alleles
1 , Panel A, Lane 6 compared ...
Brilliant Core Reagent Kits for QPCR and QRT-PCR
individual. One way of calling alleles
is by comparing Ct values. In a
sample containing the wild-type alleles
only, the TET signal should be
... 3 ).
Another method for calling alleles
is to examine the fluorescence at
Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
and the availability of TaqMan assays for SNP analysis of other alleles
in the CYP2D6 gene and other genes, demonstrate that the ... E. Rapid detection
of the CYP2D6*3, CYP2D6*4, CYP2D6*6 alleles
by tetra-primer PCR and of
the CYP2D6*5 allele by multiplex ...
Enhanced siRNA Delivery and Long-term Gene Silencing
... the effects seen
with heterogeneous disease causing alleles
such as BRCA1,
where only one of the alleles
needs to be mutated to see a
disease phenotype, and ...
Healthcare slow to adopt genetic advances
... are being addressed now, that are emerging as being important areas of personalized medicine, are those associated with a few allele interactions or alleles
of importance," he said, "which help detect those interactions, those biochemical interactions, that are probably leading to the disease, but for ...
Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems
... sample (Figure 1).
Polymorphisms in band patterns map to specific loci, allowing individuals to be genotyped or differentiated based on the alleles
Performing Successful AFLP Analysis
The success of an AFLP assay depends on three factors:
Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes
in 16S rDNA sequence that result in different G+C content among closely-related
bacterial species as well as among multiple alleles
of the same gene in
a single species. Separation based on subtle differences in G+C content
by DGGE into discrete visible bands ...
Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System
... silent mutation (Figure 3,
lane 2) were detected.
Detection of naturally mutant alleles
of candidate genes and a study of
their consequent effects can be expected to provide important clues to
the precise ...
Detection of Mutations in the CYP21 Gene Using the DCode System
... for exon 7 the Val281Leu mutation.
To perform complete genotyping of 21-hydroxylase disease alleles
carrier diagnosis, it is essential to use a technique that not only detects
the most frequent previously ...
Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System
... of transformed plant cells for quantitative scoring
of single-cell traits.
Mutation induced recessive alleles
(mlo) of the barley Mlo
gene confer broad spectrum resistance against Blumeria graminis f. sp.
hordei, the causal agent of ...
Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
... asymmetric PCR with a Cy5-labeled primer
to produce the Cy5-labeled strand in excess. The wild-type, mutant, and
were then detected with a single-labeled FAM probe
that hybridized to the amplified Cy5-labeled target strands. Melt-curve
Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
... were examined. The LDH intron appeared to be
virtually invariant, with only a single variant encountered among the
screened. The cyt b fragment was likewise nearly monomorphic;
70 individuals possessed the common haplotype and two individuals were
SeeSNP Propietary Analysis Advantages
... Genome Research , Mar 2005; 15: 436442.
5) C Korch and H Drabkin. Improved DNA sequencing accuracy
and detection of heterozygous alleles
citrate and different fluorescent dye terminators, Genome
Research , Jun 1999; 9: 588595.
6) AB ...
Fully automated DNA purification and efficient multiplex PCR for analysis
of microsatellite loci
... Donors ").
All 6 targets were well amplified from all DNA samples, irrespective of
whether the locus was homozygous (both alleles
generate a fragment of
the same size) or heterozygous (fragments of different size are generated
from each allele).
The use of the Mastercycler gradient for single-cell PCR and preimplantation
single lymphoblasts showed amplification (Figure 1 2). Of these, 4/23 (17%)
showed allele drop-out (ADO), i.e. one of both alleles
in a heterozygous
cell was not amplified (Table 2). Similar results were obtained for the
detection of DF508 using fluorescent
Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon
Allelic Discrimination Kits
DNA polymerase, and the optimized PCR buffer. Amplification is carried
out in a spectrofluorometric thermal cycler. The alleles
in a DNA sample
are determined by comparing the endpoint fluorescence value ( Figure
3 ) and/or the threshold cycle (Ct) value ...