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Alleles in Biological Technology

Transgenomic Completes Licensing Option With Dana-Farber Cancer Institute on Cold-PCR for Enrichment of DNA Mutations

... and reliable tool in analyzing DNA variations, especially where the mutant alleles are at a very low concentration within the sample (less than 1% of the ... Cold-PCR protocols preferentially amplify heteroduplexes such that mutant alleles become enriched compared to normal alleles. The range of enrichment ...

Honey bee invaders exploit the genetic resources of their predecessors

... from throughout the Africanized honey bee genome showed that most of the alleles were common to African honey bees. But of the alleles common to European bees, those found in functional parts of the genome (in ...

ProImmune Launches New MHC-Peptide Binding Assay for Class II HLA Alleles

... available from the Company. The Class II REVEAL(TM) binding assay for HLA alleles DR1-4, is a powerful cell-free in vitro assay that can rapidly predict ... extension of its range of MHC Class II Ultimer(TM) staining reagents with alleles DR2 and DR3 to cover HLA DR1 to 4. Class II Ultimers(TM) can be used ...

New Study Raises Questions About Prostate Cancer Therapies Targeting Insulin-Like Growth Factor Receptor

... IGF1 signaling. To test their reasoning they conducted a second experiment by crossing mice carrying the prostate-specific IGF-1R knockout alleles with transgenic mice that develop spontaneous prostate cancer when p53 and select other genes are compromised. The results were as predicted: ...

SNPs Powerful Tools for Association Studies

... to 28 human samples) that revealed seven alleles at frequencies of 48%, 25%, 12%, 5%, 3%, 3%, and ... bi-allelic (i.e., there are only two possible alleles at the locus). Because people have two copies of ... although this is extremely rare for minor alleles that occur at low frequencies. In the Applera ...

Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System

... (DGGE). When separation of HLA-DRB alleles is followed by direct sequencing, this ... rapid way of identifying all HLA-DRB alleles for high-resolution tissue typing. ... had been typed previously for HLA-DRB alleles using both PCR-SSCP and cloning and sequencing ...

Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System

... network of control alleles, 22 of these alleles can be rapidly and accurately resolved ... the amplified chinook salmon A1 chinook alleles (5, 9, and 10), differing from one ... curves indicated that all three A1 alleles melted at different concentrations of denaturant ...

Superior Gene Silencing Using Adenoviral Vectors

... Reduce Expression from Transgenic alleles in vivo To demonstrate that ... type 3. In 70% of diseased carriers, alleles contain a G to C mutation. Dr. ... or mutant polyglutamine (polyQ) alleles (Atx-Q166-GFP) containing a CAG ...

Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori

... all mPCR reactions including all involved alleles showed the separation of seven different ... with possible disease outcome are the various alleles of the vacA gene, the presence of the cagA gene ... the combined analysis of the five different alleles (vacA s1 or s2, cagA, vacA m1 or m2) ...

QIAGEN Multiplex PCR Handbook

... 90 seconds to 3 minutes. However, some alleles may not be detectable when using low ... are often highly polymorphic, with many different alleles present within a population. alleles may also differ in the number of copies of a ...

Map and Link Human Genetic Disorders with SSLP Analysis

... Quickly identify closely related alleles with the CastAway system Michelle L. ... polymorphisms), or microsatellites. Different alleles that contain these polymorphisms can vary by just ... 2-bp differences between the upper alleles ( Figure 1 , Panel A, Lane 6 compared ...

Brilliant Core Reagent Kits for QPCR and QRT-PCR

... mutant individual. One way of calling alleles is by comparing Ct values. In a sample containing the wild-type alleles only, the TET signal should be ... 3 ). Another method for calling alleles is to examine the fluorescence at the ...

Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System

... and the availability of TaqMan assays for SNP analysis of other alleles in the CYP2D6 gene and other genes, demonstrate that the ... E. Rapid detection of the CYP2D6*3, CYP2D6*4, CYP2D6*6 alleles by tetra-primer PCR and of the CYP2D6*5 allele by multiplex ...

Enhanced siRNA Delivery and Long-term Gene Silencing

... the effects seen with heterogeneous disease causing alleles such as BRCA1, where only one of the alleles needs to be mutated to see a disease phenotype, and ...

Healthcare slow to adopt genetic advances

... are being addressed now, that are emerging as being important areas of personalized medicine, are those associated with a few allele interactions or alleles of importance," he said, "which help detect those interactions, those biochemical interactions, that are probably leading to the disease, but for ...

Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems

... sample (Figure 1). Polymorphisms in band patterns map to specific loci, allowing individuals to be genotyped or differentiated based on the alleles they carry. Performing Successful AFLP Analysis The success of an AFLP assay depends on three factors: ....

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

... in 16S rDNA sequence that result in different G+C content among closely-related bacterial species as well as among multiple alleles of the same gene in a single species. Separation based on subtle differences in G+C content by DGGE into discrete visible bands ...

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

... silent mutation (Figure 3, lane 2) were detected. Discussion Detection of naturally mutant alleles of candidate genes and a study of their consequent effects can be expected to provide important clues to the precise ...

Detection of Mutations in the CYP21 Gene Using the DCode System

... for exon 7 the Val281Leu mutation. Discussion To perform complete genotyping of 21-hydroxylase disease alleles and reliable carrier diagnosis, it is essential to use a technique that not only detects the most frequent previously ...

Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System

... of transformed plant cells for quantitative scoring of single-cell traits. Introduction Mutation induced recessive alleles (mlo) of the barley Mlo gene confer broad spectrum resistance against Blumeria graminis f. sp. hordei, the causal agent of ...

Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A

... asymmetric PCR with a Cy5-labeled primer to produce the Cy5-labeled strand in excess. The wild-type, mutant, and heterozygous alleles were then detected with a single-labeled FAM probe that hybridized to the amplified Cy5-labeled target strands. Melt-curve ...

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

... were examined. The LDH intron appeared to be virtually invariant, with only a single variant encountered among the 144 alleles screened. The cyt b fragment was likewise nearly monomorphic; 70 individuals possessed the common haplotype and two individuals were ...

SeeSNP Propietary Analysis Advantages

... Genome Research , Mar 2005; 15: 436442. 5) C Korch and H Drabkin. Improved DNA sequencing accuracy and detection of heterozygous alleles using manganese citrate and different fluorescent dye terminators, Genome Research , Jun 1999; 9: 588595. 6) AB ...

Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci

... Donors "). All 6 targets were well amplified from all DNA samples, irrespective of whether the locus was homozygous (both alleles generate a fragment of the same size) or heterozygous (fragments of different size are generated from each allele). ...

The use of the Mastercycler gradient for single-cell PCR and preimplantation diagnosis

... (88%) single lymphoblasts showed amplification (Figure 1 2). Of these, 4/23 (17%) showed allele drop-out (ADO), i.e. one of both alleles in a heterozygous cell was not amplified (Table 2). Similar results were obtained for the detection of DF508 using fluorescent ...

Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits

... DNA polymerase, and the optimized PCR buffer. Amplification is carried out in a spectrofluorometric thermal cycler. The alleles in a DNA sample are determined by comparing the endpoint fluorescence value ( Figure 3 ) and/or the threshold cycle (Ct) value ...
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