Transgenomic Completes Licensing Option With Dana-Farber Cancer Institute on Cold-PCR for Enrichment of DNA Mutations
... low concentration within the sample (less than 1% of the total wild type allele
concentration). Cold-PCR protocols preferentially amplify heteroduplexes ... to Transgenomic's target of achieving a 1/10,000 mutant to normal allele
ratio detection in a routine, cost-effective and high throughput protocol. ...
Matrix Genomics, Elizabeth Corder, PhD - Gene Variation in the LRRK2 Gene and High Risk for Parkinson's Disease
... author on the Science article that described how risk for Alzheimer's disease multiplied according to the number of copies of the apolipoprotein E allele
4 inherited from parents. This finding has been replicated in hundreds of studies and remains the one established genetic risk factor for Alzheimer's ...
deCODE and Celera Partner to Expand use of deCODE Risk Markers for Heart Attack, Stroke and Diabetes
... 9p21 affects
the risk of myocardial infarction," Science (2007) 8;316 (5830):1491-3.
2. Brautbar, A, et al., "Impact of adding a single allele
in the 9p21
locus to traditional risk factors on reclassification of coronary heart
disease risk and implications for lipid-modifying therapy in ...
Polycystic Kidney Disease Assay at Cornell University Uses Transgenomic's Surveyor Nuclease & WAVE Technology
... reduced. Additionally, information is gained about the site of a mutation, allowing more accurate DNA sequence analysis when any given mutant allele
is present at below 25% compared to the normal allele. SURVEYOR Nuclease's effectiveness in disease specific mutation discovery has been well ...
Gleevec Receives FDA Priority Review as First Therapy to Reduce Recurrence of Gastrointestinal Stromal Tumors After Surgery
... (HES) and/or
chronic eosinophilic leukemia (CEL) who have the FIP1L1- PDGFR alpha fusion
kinase (mutational analysis or FISH demonstration of CHIC2 allele
and for patients with HES and/or CEL who are FIP1L1-PDGFR alpha fusion
kinase-negative or unknown; adult patients with unresectable, ...
SNPs Powerful Tools for Association Studies
... SNPs are usually only present in two forms, the allele
that is more rare (i.e., present in less than 50% of the population) is referred to as the minor allele
frequencies of SNPs vary greatly, in part ...
Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
... Engine Opticon 2 system to genotype the CYP2D6*4 allele
patient samples. Two hydrolysis probes, ... in DNA from 211 patients.
The *4 allele
is a SNP at position 1934 (GenBank Accession ... homozygous for the variant A
who would be poor metabolizers of many clinical ...
Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
... location of the 2 codon insert, present in allele
10, but absent
9. Thus, it appeared that the 2 codon insert and ...
For the homoduplex A1 allele
fragments, increasing migration
Superior Gene Silencing Using Adenoviral Vectors
... targeting either the wild type or the mutant allele
were designed generated
from these ... the
fusion construct containing that allele
(Atx-Q28-GFP, Figure 3A). Conversely,
... suppressed expression of the mutant allele
(Atx-Q166, Figure 3A). ...
The use of the Mastercycler gradient for single-cell PCR and preimplantation
... middle band re p resents the healthy allele
(three bp larger), and
the top ... cell in lane 7 shows ADO, i.e. only the healthy allele
is amplified, such that this cell ... 1 2). Of these, 4/23 (17%)
drop-out (ADO), i.e. one of both alleles in a ...
Brilliant Core Reagent Kits for QPCR and QRT-PCR
was complementary to the mutant allele
(A), and the TET probe was complementary
to the wild-type allele
(G). We included the passive reference dye, ... homozygote wild type or mutant because only one allele
each (i.e., the
wild-type and the ...
Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon
Allelic Discrimination Kits
... variants (Allele 1/Allele 1,
Allele 2/Allele 2, allele
1/Allele 2) can be distinguished simultaneously ... The molecular beacon specific to the wild-type allele
labeled with the fluorophore ...
beacon specific to the mutant allele
is labeled with the fluorophore ...
Healthcare slow to adopt genetic advances
... a predisposition to cystic fibrosis and other single-allele diseases - an allele
is a member of a pair of genes that occupy a specific position on a ... important areas of personalized medicine, are those associated with a few allele
interactions or alleles of importance," he said, "which help detect those ...
Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
... each unknown sample as mutant, wild type, or heterozygote
of mutant and one allele
of wild-type sequence).
Map and Link Human Genetic Disorders with SSLP Analysis
1 , Panel A) were identified for this marker, with each allele
by at least 2 bp, representing multiples of (CA) dinucleotides. Each allele
is represented by multiple bands (stutter bands), which may
Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)
... upon Hybridization. Nature Biotechnology 14: 303308.
2. Tyagi, S., D.P. Bratu and F.R. Kramer. (1998) Multicolor molecular beacons for allele
discrimination. Nature Biotechnology 16: 4953.
3. Marras, S.A.E., F.R. Kramer and S. Tyagi. (1999) Multiplex detection of single-nucleotide ...
Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems
... The use of the POP-7 polymer offers advantages such as high resolution, especially for large fragment sizes, which should result in more accurate allele
calling by GeneMapper software, and superior sizing precision (Figure 2) with reduced run times.
Amplification of genome-representative DNA from limited sources with GenomePlex WGA technology for use in genetic alterations studies
... and accurate method for amplifying nanogram quantities of starting material from any source, resulting in microgram amounts of DNA with no detectable allele
bias. The GenomePlex WGA technology is time-efficient, allowing the researchers to amplify DNA in less than three hours. The technology is based on ...
Transformation of Nematodes With the Helios Gene Gun
were screened 3 to 4 days later for visible dominant mutant marker rol-6
(Kramer et al., 1990 ). Animals carrying roller allele
a helically twisted cuticle and body. Muscle contractions, which would
normally propel the worm forward or ...
Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
... gold particles coated
with pBC17 (Goff, et al., 1990), a plasmid which encodes genes for the
C1 regulatory gene and the B-Peru allele
of the B regulatory gene in the
maize anthocyanin biosynthetic pathway. When expressed together, these
genes activate this ...
Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System
Results and Discussion
Detached leaves of mlo resistant barley cultivar Ingrid containing
the null mutant allele
mlo-5 were bombarded with plasmid DNA of
pUGLUM as described in Methods (Figure 1). The plasmid harbors the Mlo
wild type and ...
The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B
... probe. For the analysis of a biallelic genetic locus, two allele-specific
probes are synthesized: a probe specific for the detection of allele
labeled with one dye, and a second probe specific for the other allelic
variant labeled with a different dye. Proper choice ...
Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
... when the GC-clamp was positioned
at the 3 side of the PCR product and suggested that the optimal denaturing
gradient for allele
separation ranged from 4065%. Thus, our PCR products
were electrophoresed on a 4065% parallel denaturing gradient. Figures
Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System
... because it provides
high throughput analysis. The detection of mutation was
unclear in some cases where the rate of mutated allele
low or the genomic DNA was extracted from paraffin
embedded materials. In such cases we used SYBR Green I
Designing Controls for siRNA
... silencing effects (1,2). In fact,
this remarkable specificity has been shown in a model system
to permit allele
specific gene silencing (2). In contrast,
another report indicates that siRNAs with regions of 14-15
QIAGEN Multiplex PCR Handbook
... PCR according to the instructions of the detection instruments
Faint peaks or no allele
Poor capillary electrophoresis
injection (size standard also affected)
Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
... is first detectable above background. For the three assays
described in the article, a Ct value of 20 to 32 indicates the presence of a
and a Ct value of 40 indicates the absence of a specific allele.
The maximum Ct value is 40 as PCR proceeds to 40 cycles.
The Specific Kits