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Allele in Biological Technology

Transgenomic Completes Licensing Option With Dana-Farber Cancer Institute on Cold-PCR for Enrichment of DNA Mutations

... low concentration within the sample (less than 1% of the total wild type allele concentration). Cold-PCR protocols preferentially amplify heteroduplexes ... to Transgenomic's target of achieving a 1/10,000 mutant to normal allele ratio detection in a routine, cost-effective and high throughput protocol. ...

Matrix Genomics, Elizabeth Corder, PhD - Gene Variation in the LRRK2 Gene and High Risk for Parkinson's Disease

... author on the Science article that described how risk for Alzheimer's disease multiplied according to the number of copies of the apolipoprotein E allele 4 inherited from parents. This finding has been replicated in hundreds of studies and remains the one established genetic risk factor for Alzheimer's ...

deCODE and Celera Partner to Expand use of deCODE Risk Markers for Heart Attack, Stroke and Diabetes

... 9p21 affects the risk of myocardial infarction," Science (2007) 8;316 (5830):1491-3. 2. Brautbar, A, et al., "Impact of adding a single allele in the 9p21 locus to traditional risk factors on reclassification of coronary heart disease risk and implications for lipid-modifying therapy in ...

Polycystic Kidney Disease Assay at Cornell University Uses Transgenomic's Surveyor Nuclease & WAVE Technology

... reduced. Additionally, information is gained about the site of a mutation, allowing more accurate DNA sequence analysis when any given mutant allele is present at below 25% compared to the normal allele. SURVEYOR Nuclease's effectiveness in disease specific mutation discovery has been well ...

Gleevec Receives FDA Priority Review as First Therapy to Reduce Recurrence of Gastrointestinal Stromal Tumors After Surgery

... (HES) and/or chronic eosinophilic leukemia (CEL) who have the FIP1L1- PDGFR alpha fusion kinase (mutational analysis or FISH demonstration of CHIC2 allele deletion) and for patients with HES and/or CEL who are FIP1L1-PDGFR alpha fusion kinase-negative or unknown; adult patients with unresectable, ...

SNPs Powerful Tools for Association Studies

... SNPs are usually only present in two forms, the allele that is more rare (i.e., present in less than 50% of the population) is referred to as the minor allele . allele frequencies of SNPs vary greatly, in part ...

Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System

... Engine Opticon 2 system to genotype the CYP2D6*4 allele in 211 patient samples. Two hydrolysis probes, ... in DNA from 211 patients. The *4 allele is a SNP at position 1934 (GenBank Accession ... homozygous for the variant A allele who would be poor metabolizers of many clinical ...

Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System

... location of the 2 codon insert, present in allele 10, but absent in allele 9. Thus, it appeared that the 2 codon insert and ... For the homoduplex A1 allele fragments, increasing migration ...

Superior Gene Silencing Using Adenoviral Vectors

... targeting either the wild type or the mutant allele were designed generated from these ... the fusion construct containing that allele (Atx-Q28-GFP, Figure 3A). Conversely, ... suppressed expression of the mutant allele construct (Atx-Q166, Figure 3A). ...

The use of the Mastercycler gradient for single-cell PCR and preimplantation diagnosis

... middle band re p resents the healthy allele (three bp larger), and the top ... cell in lane 7 shows ADO, i.e. only the healthy allele is amplified, such that this cell ... 1 2). Of these, 4/23 (17%) showed allele drop-out (ADO), i.e. one of both alleles in a ...

Brilliant Core Reagent Kits for QPCR and QRT-PCR

... probe was complementary to the mutant allele (A), and the TET probe was complementary to the wild-type allele (G). We included the passive reference dye, ... homozygote wild type or mutant because only one allele each (i.e., the wild-type and the ...

Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits

... variants (Allele 1/Allele 1, Allele 2/Allele 2, allele 1/Allele 2) can be distinguished simultaneously ... The molecular beacon specific to the wild-type allele is labeled with the fluorophore ... beacon specific to the mutant allele is labeled with the fluorophore ...

Healthcare slow to adopt genetic advances

... a predisposition to cystic fibrosis and other single-allele diseases - an allele is a member of a pair of genes that occupy a specific position on a ... important areas of personalized medicine, are those associated with a few allele interactions or alleles of importance," he said, "which help detect those ...

Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A

... each unknown sample as mutant, wild type, or heterozygote (one allele of mutant and one allele of wild-type sequence). Methods ...

Map and Link Human Genetic Disorders with SSLP Analysis

... Figure 1 , Panel A) were identified for this marker, with each allele differing by at least 2 bp, representing multiples of (CA) dinucleotides. Each allele is represented by multiple bands (stutter bands), which may ...

Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)

... upon Hybridization. Nature Biotechnology 14: 303308. 2. Tyagi, S., D.P. Bratu and F.R. Kramer. (1998) Multicolor molecular beacons for allele discrimination. Nature Biotechnology 16: 4953. 3. Marras, S.A.E., F.R. Kramer and S. Tyagi. (1999) Multiplex detection of single-nucleotide ...

Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems

... The use of the POP-7 polymer offers advantages such as high resolution, especially for large fragment sizes, which should result in more accurate allele calling by GeneMapper software, and superior sizing precision (Figure 2) with reduced run times. Conclusion The ...

Amplification of genome-representative DNA from limited sources with GenomePlex WGA technology for use in genetic alterations studies

... and accurate method for amplifying nanogram quantities of starting material from any source, resulting in microgram amounts of DNA with no detectable allele bias. The GenomePlex WGA technology is time-efficient, allowing the researchers to amplify DNA in less than three hours. The technology is based on ...

Transformation of Nematodes With the Helios Gene Gun

... were screened 3 to 4 days later for visible dominant mutant marker rol-6 (Kramer et al., 1990 ). Animals carrying roller allele su-1006 exhibit a helically twisted cuticle and body. Muscle contractions, which would normally propel the worm forward or ...

Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

... gold particles coated with pBC17 (Goff, et al., 1990), a plasmid which encodes genes for the C1 regulatory gene and the B-Peru allele of the B regulatory gene in the maize anthocyanin biosynthetic pathway. When expressed together, these genes activate this ...

Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System

... Results and Discussion Detached leaves of mlo resistant barley cultivar Ingrid containing the null mutant allele mlo-5 were bombarded with plasmid DNA of pUGLUM as described in Methods (Figure 1). The plasmid harbors the Mlo wild type and ...

The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B

... probe. For the analysis of a biallelic genetic locus, two allele-specific probes are synthesized: a probe specific for the detection of allele 1 labeled with one dye, and a second probe specific for the other allelic variant labeled with a different dye. Proper choice ...

Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System

... when the GC-clamp was positioned at the 3 side of the PCR product and suggested that the optimal denaturing gradient for allele separation ranged from 4065%. Thus, our PCR products were electrophoresed on a 4065% parallel denaturing gradient. Figures 2a ...

Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System

... because it provides high throughput analysis. The detection of mutation was unclear in some cases where the rate of mutated allele was low or the genomic DNA was extracted from paraffin embedded materials. In such cases we used SYBR Green I instead ...

Designing Controls for siRNA Experiments

... silencing effects (1,2). In fact, this remarkable specificity has been shown in a model system to permit allele specific gene silencing (2). In contrast, another report indicates that siRNAs with regions of 14-15 ...

QIAGEN Multiplex PCR Handbook

... PCR according to the instructions of the detection instruments manufacturer. Faint peaks or no allele peaks 1. Poor capillary electrophoresis injection (size standard also affected) ...

Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations

... is first detectable above background. For the three assays described in the article, a Ct value of 20 to 32 indicates the presence of a specific allele and a Ct value of 40 indicates the absence of a specific allele. The maximum Ct value is 40 as PCR proceeds to 40 cycles. The Specific Kits ...
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