Transcription in Biological Products
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Transcription in Biological Products
MAXIscript SP6/T7 Kit from Ambion
Description:... Ambions MAXIscript In Vitro transcription Kits synthesize RNA probes with specific ... g/20ul reaction) of up to 2 kb. Ambions in vitro transcription kits are the most widely used in vitro transcription kits in the United ....Company:Ambion
TURBO DNase (2 U/l) from Ambion
Description:... the efficiency of DNA removal from in vitro transcription buffer, as shown in Figure 1. In this case, TURBO ... using real-time RT-PCR (Table 2). When in vitro transcription reactions are treated with either DNase I or ... choice for eliminating plasmid DNA templates from transcription reactions, particularly for the synthesis of RNA ...Company:Ambion
pMIR-hU6-Luc, Human U6 Luciferase Control Vector from Mirus Bio Corporation
Description:... of expression constructs that drive siRNA transcription in the nucleus from RNA Polymerase III promoters. ... is a DNA construct that includes a complete transcription unit consisting of a promoter, a template sequence to be transcribed by RNA polymerase, and a transcription termination signal. An siRNA expression cassette ...Company:Mirus Bio Corporation
pMIR-mU6-Luc, Mouse U6 Luciferase Control Vector from Mirus Bio Corporation
Description:... of expression constructs that drive siRNA transcription in the nucleus from RNA Polymerase III promoters. ... is a DNA construct that includes a complete transcription unit consisting of a promoter, a template sequence to be transcribed by RNA polymerase, and a transcription termination signal. An siRNA expression cassette ...Company:Mirus Bio Corporation
Goat Anti-BRF2 Polyclonal Antibody, Unconjugated from GeneTex
Description:... BRF2 is one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. BRF2 is ...Company:GeneTex
ULS cDNA Synthesis and Labeling Kit (with Biotin) from KREATECH Biotechnology
Description:... hybridization experiments. First unmodified cDNA is made via a reverse transcription from mRNA into cDNA using an Oligo(dT) primer. Unlike other methods, there are no unnatural modified nucleotides used in the reverse transcription reaction. This unmodified cDNA is then labeled in a one step 30 min. ...Company:KREATECH Biotechnology
ULS cDNA Synthesis and Labeling Kit (with Cy3 and Cy5) from KREATECH Biotechnology
Description:... hybridization experiments. First unmodified cDNA is made via a reverse transcription from mRNA into cDNA using an Oligo(dT) primer. Unlike other methods, there are no unnatural modified nucleotides used in the reverse transcription reaction. This unmodified cDNA is then labeled in a one step 30 min. ...Company:KREATECH Biotechnology
m7G(5)ppp(5)G (Cap Analog) from Ambion
Description:... Analog) is used for the synthesis of 5' capped RNA molecules in in vitro transcription reactions. Substitution of cap analog for a large portion of the GTP in an in vitro transcription reaction results in the incorporation of the cap structure into a large ...Company:Ambion
m7G(5)ppp(5)G (Cap Analog) from Ambion
Description:... Analog) is used for the synthesis of 5' capped RNA molecules in in vitro transcription reactions. Substitution of cap analog for a large portion of the GTP in an in vitro transcription reaction results in the incorporation of the cap structure into a large ...Company:Ambion
ABsolute QRT-PCR SYBR Green Fluorescein Mix from ABgene
Description:... during the QPCR stage of the reaction. The AMV component allows reverse transcription to be carried out at up to 57C when secondary structure is problematic. A ... Thermo-Start DNA Polymerase remains completely inactive during the reverse transcription stage of QRT-PCR. Enzyme is fully activated by a 15minute activation at ...Company:ABgene
BL21 Star (DE3) One Shot Chemically Competent E. coli from Invitrogen
Description:... yield in T7 promoter-based expression systems (Figure 1). Because the T7 RNA polymerase synthesizes mRNA more rapidly than E. coli RNA polymerases transcription from the T7 ...Company:Invitrogen
Description:... dsDNA samples. The DNA that is recovered from the Microfilter Cartridge is ready for any application that requires highly pure DNA including in vitro transcription reactions. The DNAclear Kit includes enough materials to perform 20 ....
Company:Ambion
mCD-1 Multi-Probe Template Set from BD Biosciences Pharmingen
Description:... mCD-1 10 transcription ...Company:BD Biosciences Pharmingen
MEGAscript RNAi Kit from Ambion
Description:... mass amounts of ready-to-use dsRNA for RNAi experiments in non-mammalian systems. The MEGAscript RNAi Kit is based on Ambions patented high-yield transcription technol-ogy and includes reagents for transcription, nuclease digestion and clean ....Company:Ambion
Topoisomerase I (Vaccinia) from Molecular Cloning Laboratories (MCLAB)
Description:... Ideal for relax positively and negatively supercoiled DNA in presence of EDTA Applications Studying the effects of supercoiling on transcription in vitro Studying chromatin reconstitution in vitro Determining the degree of supercoiling of naturally occurring DNA Detecting mutant ...Company:Molecular Cloning Laboratories (MCLAB)
Rabbit Anti-Human POU2F2 Polyclonal Antibody, Unconjugated from Proteintech Group, Inc.
Description:... POU domain, class 2, transcription factor ...Company:Proteintech Group, Inc.
GeneRacer 3' Nested Primer from Invitrogen
Description:... during cDNA synthesis. This increases the size and yield of cDNA. SuperScript II RT is more thermostable than wild-type RTS. This enables reverse transcription at higher temperatures, relaxing secondary structure of complex templates, and allowing cDNA synthesis to go to ....Company:Invitrogen
Rabbit Anti-ATF4 Polyclonal Antibody, Unconjugated from Proteintech Group, Inc.
Description:... activating transcription factor 4 (tax-responsive enhancer element ...Company:Proteintech Group, Inc.
Mouse Anti-Neurogenin-2 Monoclonal Antibody, Unconjugated from R&D Systems
Description:... Human/Rat Neurogenin-2 MAb (Clone 7G4) Protein Family: Neural Crest Cell Markers, transcription ...Company:R&D Systems
PS20 and RS100 preactivated ProteinChip Arrays from Ciphergen
Description:... with one column of a standard 96-well microplate (9 mm well pitch). Typical applications include immunoassays, receptor-ligand binding studies and transcription factor ....Company:Ciphergen
Goat Anti-PAX3 Polyclonal Antibody, Unconjugated from GeneTex
Description:... Goat polyclonal to PAX3 This protein is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These proteins play critical roles during ...Company:GeneTex
Description:... of TBP occurs. This antibody does not inhibit the activity of TBP (prepared either as a nuclear extract or as a minimal activity extract) in a DNA transcription assay. This antibody is effective in immunoblotting and DNA supershift assays. *Predicted MW = 38 ....
Company:Covance Research Products, Inc
ProteoJuice Protein Transfection Reagent from Novagen
Description:... of a protein into cells permits studies of protein-protein interactions, protein turnover, cell signaling pathways, apoptotic pathways, and transcription factor-mediated gene regulation. The most rapid method for studying the effects of a specific protein in a mammalian cell is to transfect the protein ...Company:Novagen
pBICEP-CMV-3 Expression Vector from Sigma-Aldrich
Description:... N-terminal FLAG® fusion and a second gene of interest or selection marker from bicistronic mRNA. Two genes can be cloned into MCS1 and MCS2 for transcription of a single message from the CMV promoter. This vector is useful for protein-protein interaction studies, multi-subunit proteins, and cloning a ...Company:Sigma-Aldrich
Mouse Anti-HDAC8 Monoclonal Antibody, Unconjugated, Clone HDAC8-48 from GeneTex
Description:... Class II HDACs have been implicated in the regulation of muscle differentiation. Interaction of HDAC4, 5, and 7 with members of the MEF2 family of transcription factors represses their transcriptional activity and prevents myogenesis. The deacetylase activity of class II HDACs is regulated by subcellular ...Company:GeneTex
Mouse Anti-CDX-2 Monoclonal Antibody, Unconjugated, Clone CDX2-88 from BioGenex
Description:... CDX2, a member of the caudal-related homeobox family, is an intestine-specific transcription factor that regulates both proliferation and differentiation in intestinal epithelial cells. It plays an important role in triggering cells towards ...Company:BioGenex
loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
Description:... The prokaryotic promoter gb2 driving the gene for ampicillin resistance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by ...Company:Gene Bridges GmbH
FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
Description:... The prokaryotic promoter gb2 driving the gene for ampicillin resistance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by ...Company:Gene Bridges GmbH
loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
Description:... The prokaryotic promoter gb2 driving the gene for chloramphenicol resistance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the chloramphenicol expression. The cassette is ...Company:Gene Bridges GmbH
Description:... of neomycin resistance in mammalian cells. The prokaryotic promoter gb2 is a slightly modified version of the Em7 promoter; it mediates higher transcription efficiency than the generally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as the eukaryotic promoter. A ...
Company:Gene Bridges GmbH
Description:... of neomycin resistance in mammalian cells. The prokaryotic promoter gb2 is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic ...
Company:Gene Bridges GmbH
FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
Description:... of neomycin resistance in mammalian cells. The prokaryotic promoter gb2 is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic ...Company:Gene Bridges GmbH
HEEBO Human Genome Set 200 pmol from Invitrogen
Description:... thousands of genes. Developed in collaboration with leading researchers at Stanford University and UCSF, the sets enable the study of human and mouse transcription patterns, and as broadly as possible, alternative splicing. An exon-centric design allows the differentiation of constitutively expressed versus ...Company:Invitrogen
Description:... This region is just C-terminal to the DNA binding domain of the VDR.Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) ...
Company:ABR-Affinity BioReagents
GeneAmp AccuRT RNA PCR Kit with Controls from Applied Biosystems
Description:... A thermostable, thermoactive designer enzyme that enables RT-PCR in a one-step, single-tube-reaction. AccuRT RNA PCR enzyme allows reverse transcription and PCR amplification to be performed at elevated temperatures (65C), which helps to remove secondary structures from the template and results in ...Company:Applied Biosystems
Description:... including RNase A, B, C, 1 and T1. SUPERaseIn can be used in any application where RNase contamination could be problematic. It is ideal for in vitro transcription and translation, cDNA synthesis, RT-PCR, and preparation of RNase-free antibodies. Because it inhibits a broader range of RNases than traditional ...
Company:Ambion
Description:... or other reducing agents. ANTI-RNase may be used in any application where placental ribonuclease inhibitor is used. It is ideal for use in in vitro transcription and translation, cDNA synthesis PCR, RT-PCR, RNA isolation procedures and the preparation of RNase-free ....
Company:Ambion
RNase Inhibitor (Cloned) from Ambion
Description:... been shown to be useful whenever the integrity of RNA must be maintained such as in the preparation of cDNA by reverse transcription, in vitro RNA transcription and in in vitro protein synthesis, and RNA isolation and preparation of RNase-free antibodies. Ribonuclease inhibitor does not inhibit RNase 1, T1, ...Company:Ambion
GeneChoice RNA Safe Recombinant Ribonuclease Inhibitor from GeneChoice, Inc.
Description:... RNA during cDNA synthesis and in vitro transcription/translation. Applications: First and second strand cDNA synthesis, RT-PCR, and in vitro transcription and translation. Unit Definition: One unit of protein inhibits the activity of 5 ng RNase A by 50%. Enzyme Concentration: 40 to 50 ...Company:GeneChoice, Inc.
ARCA (Anti Reverse Cap Analog) from Ambion
Description:... modified cap analog in which the 3 OH group (closer to m7G) is replaced with OCH3. Because of this substitution, the RNA polymerase can only initiate transcription with the remaining hydroxyl group thus forcing ARCA incorporation in the forward orientation. As a result, unlike transcripts synthesized with ...Company:Ambion
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Breaking Biology News(10 mins):Yehezkel Ben-Ari, winner of the 2009 INSERM Grand Prix 2Yehezkel Ben-Ari, winner of the 2009 INSERM Grand Prix 3New forensic technique gives clues about sharks from bite damage 2New forensic technique gives clues about sharks from bite damage 3Innovation puts next-generation solar cells on the horizon 2Researchers estimate risk of transmission of Huntingtons disease to offspring among male carriers 48309 1Researchers estimate risk of transmission of Huntingtons disease to offspring among male carriers 48309 2VisionDirect com Offers Contact Lens Customers a New Way to Pay 48307 1VisionDirect com Offers Contact Lens Customers a New Way to Pay 48307 2VisionDirect com Offers Contact Lens Customers a New Way to Pay 48307 3Accumetrics Inc Announces Distribution Channels in Israel and Cyprus 48302 1Accumetrics Inc Announces Distribution Channels in Israel and Cyprus 48302 2(Date:12/2/2009)...fe remains unclear, researchers say , , ... suggests that enzyme replacement therapy can make...bry disease, a rare illness that affects the kidne..., linked to the lack of an enzyme that helps metab...ecause it boosts the risk for a stroke, heart atta...
(Date:12/2/2009)...Y., Dec. 2 Researchers ...in blood cancer research at the annual American So...s December 4-8, 2009. ,, Throughout the ASH co...) experts, including Chief Medical Officer Barton ...nt Services Hildy Dillon, and Vice President, Rese...
(Date:12/2/2009)...y federal government ends nine months of obstructi...RNewswire-USNewswire/ -- More than 2,300 healthcar...ilities across Southern California will soon vote ...Healthcare Workers (NUHW). ,, A majority of the... but SEIU delayed the election by filing frivolous...
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Breaking Medicine News(10 mins):Health News:Labor Board OKs 2,300 Kaiser Professionals to Quit SEIU and Join National Union of Healthcare Workers 2Health News:Media alert: EUROECHO 2009 2Health News:PACE Race Returns with Denver's Rudolph Ramble 2Health News:PACE Race Returns with Denver's Rudolph Ramble 3Health News:PACE Race Returns with Denver's Rudolph Ramble 4[0] Enzyme Replacement May Help in Fabry Disease[1] The Leukemia & Lymphoma Society Available to Comment on Findings Presented at American Society of Hematology Conference[0] Labor Board OKs 2,300 Kaiser Professionals to Quit SEIU and Join National Union of Healthcare Workers[0] Media alert: EUROECHO 2009[0] PACE Race Returns with Denver's Rudolph Ramble
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