pMIR-hU6-Luc, Human U6 Luciferase Control Vector from Mirus Bio Corporation
... can be cloned using either the Hind III or EcoR I site upstream of the promoter and Xho I or BamH I sitedownstream of the siRNA and termination sequence. The cloning sites used must be unique in the PCR product. Hind III andXho I are recommended because these restriction enzymes function with compatible ......u testing of RNA cellular functions. Visit our web site for more information about this product and the powerful genomic applications of OligoMix. LC Sciences LC Sciences is a genomics and proteomics company offering innovative and customizable products based on the Paraflo microfluidic technology. We ...Breeding and Maintenance (Nonhuman Primates) from Alpha Genesis, Inc.
...y facilities. In addition, AGI is equipped with on site CDC quarantine facilities. Primate housing and CDC quarantine systems are comprehensive and fully compliant with ILARs Guide for the Care and Use of Laboratory Animals. Diverse housing methods accommodate numerous requirements required by corporate ...Bovine IFN gamma ELISPOT Kit from Endogen
...tion at the single cell level. A spot forms at the site where secreted cytokine is bound, is counted (either using a dissection microscope or automated ELISPOT reader) and then compared to the number of cells added to the microplate. This allows the frequency of cells secreting the cytokine to be calculat...IMPACT-CN System from New England Biolabs
...g. Both pTYB1 and pTYB11 contain a Sap I cloning site which allows the target gene to be cloned immediately adjacent to the cleavage site of the intein tag; this results in the purification of a target protein without any extra non-native...Staccato Sciclone ELISA Station from Caliper Life Sciences
...uire long delivery times and extensive factory and site acceptance testing, the Staccato Sciclone ELISA Station is a standard product that is available with fast turn around. The system is pre-integrated and arrives mostly assembled, requiring less than one day of installation. All system components are p...ELF® 97 Cytological Labeling Kit from CHEMICON
...ightly fluorescent yellow-green precipitate at the site of enzymatic activity a process we call Enzyme-Labeled Fluorescence (ELF).1 Essentially any cellular structure that can be specifically recognized and bound to a haptenbearing ligand can be labeled using the ELF 97 Cytological Labeling Kit. For exam...Factor Xa Protease from QIAGEN
...oteins containing a Factor Xa Protease recognition site Efficient 6xHis tag cleavage (greater than 90%)......e protease which recognizes the identical cleavage site as the native enzyme (AspAspAspAspLys?) and has similar enzymatic activity (1). Novagen&'s enzyme is produced in E. coli and purified to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supp......fusion protein, which recognizes the same cleavage site as the native enzyme: LeuGluValLeuPheGln/GlyPro. T...ET-50b(+) incorporate the HRV 3C protease cleavage site in combinations with His•Tag, S•Tag, thioredoxin (Trx•Tag™), glutathione-S-tran...AcTEV Protease from Invitrogen
... specific cleavage activity at the TEV recognition site Enhanced enzyme stability for prolonged protease activity Activity over a broad temperature range (+4C to 30C) Activity over a broad pH range (pH 6.0 to 8.5) A six-histidine sequence to facilitate its removal from the digested protein sample Gr......e protease which recognizes the identical cleavage site as the native enzyme (AspAspAspAspLys↓) and has similar enzymatic activity. Novagen&'s enzyme is produced in E. coli and purified to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. S...Rabbit Anti-Peroxiredoxin 1 Polyclonal Antibody, Unconjugated from GeneTex
...rticularly amino-terminal region containing active site cysteine residue, and the thiolspecific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx6 belonging to 1-...cGMP Safe Deposit Facility from European Collection of Cell Cultures (ECACC)
...benefits of the cGMP Safe Deposit Service? Second site storage: The ECACC cGMP Safe Deposit Service enab...he requirements of cGMP by providing secure second site storage. The service is not designed to be used as the sole or primary site of storage. Monitoring: ...Qplate from Sophion Bioscience
...lly controlled measurement sites. Each measurement site contains a sophisticated liquid flow channel system that enables the fast and precise solution and compound applications necessary for high-quality (giga-ohm) patch clamp measurements.......ion by Flp recombinase. An additional single loxP site is located at the 3 end of the cassette. Unique Not I, Xho I and Hind III sites flank the cassette for convenient cloning with restriction sites. Using the provided PCR template one can easily create a FRT-PGK-gb2-neo-FRT-loxP cassette flanked by...TypeOne Restriction Inhibitor from EPICENTRE Biotechnologies
...s as a molecular decoy that blocks the DNA binding site of type I R-M enzymes and inhibits DNA cleavage....Mouse IR Cytokine Array 12plex from Pierce Biotechnology, Inc.
...etecting antibodies are added and bind to a second site on the target proteins. Excess detecting antibody is then removed and Streptavidin-DyLight 800 Fluor is added. Signal from the infrared dye is measured with the Odyssey® or Aerius Infrared Imaging Systems from LI-COR Biosciences. The...GPS -M Mutagenesis System from New England Biolabs
...epending on your interests (6-8). If the insertion site is within a translated genesegment, this will norm...null (loss of function) mutation. There is minimal site preference for insertion, so disruption of any open reading frame should be possible. Due to target ...LIC Duet™ Minimal Adaptor from Novagen
...es a T7 promoter, lac operator, ribosome binding site (rbs), and an ATG translation initation codon. This adaptor is designed for expression of a target protein with minimal vector-encoded fusion sequences....LIC Duet™ T7•Tag® Ek Adaptor from Novagen
...es a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon, and an N-terminal T7•Tag coding sequence. The T7•Tag is a small epitope tag (11 aa) that is useful for detection of the target protein with T7•Tag Monoclonal Antibody and purification using the T...LIC Duet™ GST•Tag™ Ek Adaptor from Novagen
...es a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon and an N-terminal GST•Tag coding sequence. The GST•Tag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. The GST•Tag is a multi-purpose ...LIC Duet™ Trx•Tag™ Ek Adaptor from Novagen
...es a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon, and an N-terminal Trx•Tag coding sequence. The Trx•Tag is a 109 aa sequence that encodes thioredoxin. Fusion to this sequence has been demonstrated to enhance solubility of many proteins that are typi...Staccato Cell Station - Assay from Caliper Life Sciences
...uire long delivery times and extensive factory and site acceptance testing, the Staccato Cell Station-Assay is a standard product which is available with 30 day delivery (45 day international). The system is pre-integrated and arrives mostly assembled, requiring less than one day of installation. All syst...pAcHLT-A-BFP from BD Biosciences Pharmingen
...P), followed by a 6xHis tag and a multiple cloning site (MCS).Foreign genes are expressed as BFP-6xHis fusion proteins when cloned into one of the available restriction enzymesites (EcoR I, Stu I, Sac I, Not I, Pst I, Kpn I, Sma I or Bgl II). The presence of the BFP coding sequence allows thevisualization...PTP1B Inhibitor from Calbiochem
...3 and PTP1B 298 , respectively). Binds to a novel site away from the catalytic pocket and inhibits PTP1B activity by preventing closure of the WPD loop. Purity: ≥95% by HPLC. Soluble in DMSO or EtOH. M.W. 741.5. References: Wiesmann, C., et al. 2004. Nat. Struct. Mol. Biol. 11, 730....pAcAB4 Baculovirus Transfer Vector from BD Biosciences Pharmingen
...llowed bya second p10 promoter, a Bgl II insertion site and an SV40 termination sequence. Additionally, th...e is anotherpolyhedrin promoter and BamH I cloning site downstream of the first p10 promoter. This vector allowssimultaneous expression of four foreign gene...Kilo Sequencing Deletion Kit from Takara Mirus Bio
...A, thereby progressively moving the primer binding site on the vector closer to the sequence of interest. Optimized reagent cocktails are included to ensure efficient self-circularization of the deletion subclones....PinPoint™ Vector Sequencing Primer from Promega
...). The primer hybridizes upstream of the Factor Xa site at nucleotides 325343, approximately 4050 base pairs upstream of the multiple cloning region and can be used to determine if an insert is cloned in-frame with the biotinylation purification tag of the PinPoint™ Xa Vectors. The sequence of the P...Complete Adjuvant from Santa Cruz Biotechnology, Inc.
...tract macrophages and other cells to the injection site which enhances the immuneresponse. For this reason, the Complete Freund's Adjuvant is used for the initial injections; Incomplete Freund's adjuvant is used for the subsequent boosts. Antigens (preferably in saline) are typically mixed with an equal v...Rabbit Anti-JAK2, phospho (Tyr1007) Polyclonal Antibody, Unconjugated from Abcam
...derived from human JAK2 around the phosphorylation site of tyrosine 1007 (K-E-Y P -Y-K). Entrez Gene ID: 3717 Swiss Protein ID: O60674...Rabbit Anti-Jun B, phospho (Ser79) Polyclonal Antibody, Unconjugated from Lab Vision
...derived from human JunB around the phosphorylation site of Serine 79....Goat Anti-T7 Polyclonal Antibody, Agarose Conjugated from GeneTex
... Immunogen: Goats were immunized with T7 cleavage site (MASMTGGQQMG). Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Goat anti- human T7 affinity purified antibodies were coupled to agarose beads using cyanogens bromide method. Specificity: Antibody...Rabbit Anti-MKK6, phospho (Ser207) Polyclonal Antibody, Unconjugated from Abcam
...derived from human MKK6 around the phosphorylation site of Serine 207 (V-D-S P -V-A). Entrez Gene ID: 5608 Swiss Protein ID: P52564...Rabbit Anti-ATF4, phospho (Ser245) Polyclonal Antibody, Unconjugated from Abcam
...erived from human ATF4 around the phosphorylation site of Serine 245. Entrez GeneID: 468 SWISS Protein ID: P18848...