ribonuclease

DNase I (RNase-free) (2 U/l) from Ambion

...ted cells which may contain plasmid DNA), used for ribonuclease protection assays, cDNA library contraction, and RT-PCR. DNase I requires bivalent cations (Mg2+ and Ca2+ at approximately 5 mM and 0.5 mM, respectively) for maximal activity and has a pH optimum of 7.8....

RNase Inhibitor (Cloned) from Ambion

...nt in many tissues and cell types. Addition of the ribonuclease inhibitor has been shown to be useful whenever the integrity of RNA must be maintained such as in the preparation of cDNA by reverse transcription, in vitro RNA transcription and in in vitro protein synthesis, and RNA isolation and preparation of RNa...

ANTI-RNase from Ambion

ANTI-RNase is a protein ribonuclease inhibitor that noncovalently binds and inactivates...r CL3. ANTI-RNase is distinct from human placental ribonuclease inhibitor in that it has a more robust interaction with RNase and does not release active RNase in t...

SUPERaseIn from Ambion

... The most widely used RNase inhibitor is placental ribonuclease inhibitor (RIP). SUPERaseIn is distinct from RIP i...nhibits a broader range of RNases. While placental ribonuclease inhibitors are effective only against RNase A type enzymes, SUPERaseIn also inhibits RNase 1 and T1....

StabMRT Midi Kit from RNAworks

...esis, Agilent Bioanalyser 2100, mass spectrometry, ribonuclease protection assays and Northern blotting. Protocols on line on RNAworks website A free MRT-RNA sample is available on request from RNAworks....

StabMRT Plus Mini Kit from RNAworks

...esis, Agilent Bioanalyser 2100, mass spectrometry, ribonuclease protection assays and Northern blotting. Protocols on line on RNAworks website A free MRT-RNA sample is available on request from RNAworks....

StabMRT Maxi Kit from RNAworks

...esis, Agilent Bioanalyser 2100, mass spectrometry, ribonuclease protection assays and Northern blotting. Protocols on line on RNAworks website A free MRT-RNA sample is available on request from RNAworks....

HIV Volume 2 (Biochemistry, Molecular Biology and Drug Discovery) from Promega

...ding: the HIV growth cycle; reverse transcriptase, ribonuclease H, integrase and protease. Expression of HIV proteins using baculoviruses; RNA binding assays; Tat and Rev assays; HIV/CD4 interactions; transient assays for Nef-induced down regulation of DC4 expression; Vpu and Vif; cellular assays for anti-viral d...

MAXIscript SP6/T7 Kit from Ambion

...c activity, single-stranded RNA probes are used in ribonuclease protection assays, in situ hybridization, and blot hybridizations. MAXIscript Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2-4 g/20ul reaction) of up to 2 kb. Ambions in vitro transcription kits are the most widely used...