Cleland’s Reagent from Calbiochem
...S. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. A protective agent for SH groups. Does not contain waxy surfactant found in many other preparations. Blocks the lethal and hypnotic effects of pentobarbitol. Purity: ≥97% by titration. Contaminants: Heavy metals: ≤...Cleland’s Reagent from Calbiochem
...S. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. A protective agent for SH groups. Does not contain waxy surfactant found in many other preparations. Blocks the lethal and hypnotic effects of pentobarbitol. Purity: ≥97% by titration. Contaminants: Heavy metals: ≤...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
...the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
...the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
...the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ......the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ......the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ......the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
...the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
...the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ...Varian 820-MS from Varian, Inc.
The 820-MS features Varians unique collision reaction interface (CRI) system*, which provides simple, ro...ctioninterface (CRI) system makes setup of complex reaction cell conditions a thing of the past. Simply turn on the gas flow to remove interferences. Its that s...Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.
...on the Gel Alcohol precipitation of the sequencing reaction products is not required. Simply load the reaction onto a standard sequencing gel. Flexible Labeling Components can also be used with radioactively lab...Applied Biosystems 2720 Thermal Cycler from Applied Biosystems
...h space. Practice of the patented polymerase chain reaction (PCR) process requires a license. The Applied Biosystems 2720 Thermal Cycler is an Authorized Thermal Cycler for PCR and may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR lic......the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
...the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
...the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
...the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ......the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Kidney from OriGene Technologies
...the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of ...Modern Protein Chemistry: Practical Aspects from Pierce Biotechnology, Inc.
...de includes hundreds of structures, easy-to-follow reaction schemes and current application references. More than 300 pages detail the critical aspects of protein cross-linking and conjugate preparation from selection and design of reagents, to analysis of the cross-linked species. The topics covered in Chem...RapidCycler 2 Instrument from Idaho Technology Inc.
...rature ramp rates. Inside RapidCyclers cylindrical reaction chamber, tornado-like conditions ensure both temperature uniformity and rapid heat exchange within the sample. This allows the RapidCycler to spend less than one second at the high and low temperatures and still be certain that each sample has reache...Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
...ation at 530-571 nm, emission at 590-600 nm). This reaction is catalyzed by peroxidase in a one-step, homogeneous, no wash assay system. ADHP can be utilized to detect H2O2 release from cells or enzyme coupled reactions, and it allows monitoring of additional parameters. The assay is adaptable for HTS format.......is biotinylated lysine is added to the translation reaction as a precharged epsilon-labeled biotinylated lysine-tRNA complex (Transcend tRNA) rather than a free amino acid. After SDS-PAGE and electroblotting, the biotinylated proteins can be visualized by binding either Streptavidin-Alkaline Phosphatase (Stre...UBC Fractions Extract from GeneTex
...res E2 or E3 enzymes not found in Fraction II, the reaction can be supplemented with enzymes from Fraction I. Includes complimentary fractions for 2-5 conjugation reactions depending on conditions. Type: Proteins and peptides Form: 400 g in 50 mM HEPES, pH 7.6, containing 1 mM DTT....Genome Sequencer 20 System from Roche Applied Science
... • The light resulting from the sequencing reaction travels through the back of the wells of the PicoTiterPlate device and is captured by the CCD camera....Phosphate Colorimetric Assay Kit, 'EnzoLyte from AnaSpec
...orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600-660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a s...EZ-96 DNA Methylation Kit from Zymo Research
...ation analysis. The kit is based on the three step reaction that takes place between cytosine and sodium bisul...thylation Kits innovative in-column desulphonation reaction eliminates several precipitation steps and provides researchers with consistent reaction conditions....EZ DNA Methylation Kit from Zymo Research
...ation analysis. The kit is based on the three step reaction that takes place between cytosine and sodium bisul...thylation Kits innovative in-column desulphonation reaction eliminates several precipitation steps and provides researchers with consistent reaction conditions....IMPACT-CN System from New England Biolabs
.... Addition of extra residues may help the cleavage reaction for some target proteins. pTYB2 and pTYB12 contain the same or compatible restriction sites in the multiple cloning region. This allows cloning the same target gene fragment into both vectors. pTYB1 and pTYB2 use ATG of the Nde I site in the multiple...GPDH Assay Kit from Takara Mirus Bio
...l 3-phosphate dehydrogenase catalyzes a reversible reaction between dihydroxyacetone phosphate (DHAP) and glycerol 3-phosphate, with NAD as a coenzyme. GPDH activity increases significantly during differentiation of progenitor cells into adipocytes and, thus, the activity of this enzyme has been used as an in...EZ-96 DNA Methylation Kit - Deep Well from Zymo Research
...ation analysis. The kit is based on the three step reaction that takes place between cytosine and sodium bisul...thylation Kits innovative in-column desulphonation reaction eliminates several precipitation steps and provides researchers with consistent reaction conditions....ABsolute QPCR SYBR Green ROX Mix from ABgene
...rmo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. High Sensitivity & Specificity The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase Optimised buffer containing active SYBR Green I Reproducibility All mixes un...Thermostable dUTPase from GenScript Corporation
...esence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations w...Luciferase Luminescent Assay Kit, LucLite from PerkinElmer
...ited time period due to the short half-life of the reaction (half-life approximately five minutes). This means only one plate at a time can be prepared and counted. The PerkinElmer luclite Constant Quanta assay system produces a long lived glow type signal with a half-life of several hours, which makes it id...Leukocytes, human - sonicated from AppliChem GmbH
...TE: 0.9 ml per liter of urine will give a positive reaction for leukocytes on an Ames or BMD dipstick. Certification: The Donors are of US origin and have been tested and found to be NEGATIVE for HbsAg, HCV, HIV-1 & 2, Syphilis and HIV-1 Antigen by currently approved FDA methods. THIS MATERIAL IS SOLD FOR IN...MIDAS TRAQ System with 4000 Q TRAP LC/MS/MS System from Applied Biosystems
... you to design in silico multiplexed MRM (multiple reaction monitoring) assays based on gene or protein sequences. It also enables you to rapidly identify the best peptides for any sequence-defined protein target before you need to use synthetic peptides. Shorten Your Time to Results with the MIDAS Workflow ...Hot StarTaq Master Mix Kit from QIAGEN
... * Easy handling convenient Master Mix and simple reaction setup at room temperature *Minimal optimization saving time and money...Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
...ents of Pi. The Pi is then consumed bythe MESG/PNP reaction and detected by an increase in absorbanceat 360 nm...formed over a pH range of 6.5 to 8.5.2 TheMESG/PNP reaction has been used for continuous spectrophotometricdetermination of aminoacyl-tRNA synthetase activity.3......d at 6575C does not have to be equilibrated to the reaction temperature before hydrolysis. Simply place the tube containing the molten agarose at 4247C and add AgarACE Enzyme. With slight modifications to the protocol, AgarACE Enzyme can also effectively hydrolyze non-LMP agaroses, although the higher tempera...CpGenome™ Fast DNA Modification Kit from CHEMICON
...the sample DNA was originally unmethylated, an MSP reaction product will be detectable when using the primer set (labeled as 'U') designed to be complementary to the unmethylated bisulfite converted DNA sequence. No product will be generated using a primer set (labeled as 'M') designed to be complementary to ...Vaccinia DNA Topoisomerase I from EPICENTRE Biotechnologies
...ls) from covalently closed DNA. The product of the reaction is a covalently closed, circular DNA with fewer positive or negative superhelical turns. DNA Topoisomerase I does not absolutely require Mg 2+ to function, although low concentrations of magnesium ion may increase activity. The protein has a molecul...Vaccinia DNA Topoisomerase I from EPICENTRE Biotechnologies
...ls) from covalently closed DNA. The product of the reaction is a covalently closed, circular DNA with fewer positive or negative superhelical turns. DNA Topoisomerase I does not absolutely require Mg 2+ to function, although low concentrations of magnesium ion may increase activity. The protein has a molecul...TURBO DNase (2 U/l) from Ambion
...he cleavable DNA substrate is reduced as the DNase reaction proceeds. TURBO DNase thus has a functional advantage over wild-type DNase due to its superior affinity for DNA. This is best exploited in RT-PCR applications, where even a few copies of DNA can lead to a false positive outcome by PCR. As shown in Ta...SP6 RNA Polymerase from Roche Applied Science
supplied with 10x reaction buffer...ShortCut RNase III from New England Biolabs
...tCut RNase III, used with its manganese-containing reaction buffer, converts long double-stranded RNA into a heterogeneous mix of short (1825 bp) interfering RNAs (siRNA) suitable for RNA interference in mammalian cells. 1.3 units (1 l) of ShortCut RNase III is sufficient to convert 1 g of dsRNA into siRNA su...Mango-Taq DNA Polymerase from Bioline
...tion of Taq Polymerase supplied with a 10X colored reaction buffer which allows Direct Gel-Loading...AmpliTaq Gold DNA Polymerase from Applied Biosystems
...up and the first ramp of thermal cycling (when the reaction is going through non-optimal annealing temperature...that mis-primed primers are not extended. Once the reaction reaches optimal annealing temperatures, the chemical moiety is cleaved during a heat activation step...GCpro Taq DNA Polymerase Complete System from GenScript Corporation
...q DNA Polymerase. The enzyme includes an optimized reaction buffer, which enables the amplification of GC-rich templates and problematic templates with secondary structures that interfere with the PCR process. GCpro Taq DNA Polymerase provides a very robust synthesis of longer GC-rich amplification products. ...AmpliTaq DNA Polymerase, 1,000 units with Buffer I from Applied Biosystems
...cterized enzyme available for the polymerase chain reaction (PCR) process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot. The thermal activity profile of AmpliTaq DNA Polymerase is ideal for PCR applications because its optimal acti......H: 7.5 - 7.8 Optimum Temperature: 16C for exchange reaction Requirements for Divalent Cation: Mg 2+ , Mn 2+ O...pyrophosphate exchange method of Weiss et al. The reaction mixture (300 l) contains 67mM Tris-HCl, (pH 7.8), 6.7mM MgCl 2 , 10mM DTT, 66M ATP and 3.3M radiolab...DNA Exonuclease III , E. coli from EPICENTRE Biotechnologies
...ng stretches of single-stranded DNA. Under defined reaction conditions, DNA degradation by Exonuclease III pro...bonucleotides by Exonuclease III is dependent upon reaction factors including temperature, ionic strength, template sequence, and enzyme-to-DNA ratios, each tem...T7 RNA Polymerase from Roche Applied Science
supplied with 10x reaction buffer...Human Topoisomerase II, Alpha from USB Corp.
...0C for long-term storage. Assay Conditions: The reaction mixture contains 10mM Tris-HCl, pH 7.9, 175mM KCl,...x and incubate at 3037C for 15 minutes. *Stop the reaction by the addition of 3 l of 7mM EDTA or by the addition of SDS to 1%....Human Topoisomerase II, Alpha from USB Corp.
...0C for long-term storage. Assay Conditions: The reaction mixture contains 10mM Tris-HCl, pH 7.9, 175mM KCl,...x and incubate at 3037C for 15 minutes. *Stop the reaction by the addition of 3 l of 7mM EDTA or by the addition of SDS to 1%....RNA Cap Analog, Trimethylated from EPICENTRE Biotechnologies
...on of the GTP present in an in vitro transcription reaction results in the synthesis of transcripts with a cap on the 5'-end of the RNA...RNA Cap Analog, Unmethylated from EPICENTRE Biotechnologies
...on of the GTP present in an in vitro transcription reaction results in the synthesis of transcripts with a cap on the 5'-end of the RNA...RNA Cap Analog, Methylated from EPICENTRE Biotechnologies
...on of the GTP present in an in vitro transcription reaction results in the synthesis of transcripts with a cap on the 5'-end of the RNA...m7G(5)ppp(5)G (Cap Analog) from Ambion
...ge portion of the GTP in an in vitro transcription reaction results in the incorporation of the cap structure into a large fraction of the transcripts. Capped mRNAs are generally translated more efficiently in reticulocyte lysate and wheat germ in vitro translation systems. Uncapped mRNAs are rapidly degraded......ge portion of the GTP in an in vitro transcription reaction results in the incorporation of the cap structure into a large fraction of the transcripts. Capped mRNAs are generally translated more efficiently in reticulocyte lysate and wheat germ in vitro translation systems. Uncapped mRNAs are rapidly degraded......DNA (5). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA. The CpGenome™ DNA Modification Kit (S7820) contains the reagents necessary to perform the initial bisulfite reactio...