modification

Connect from Tecan

...werwash 384 microplate washer without any hardware modification to the host instrument. Connect has no rotary movements, saving time and making all movements smooth and efficient. Connect uses the same plate cassettes as those used by the Te-Stack option so that you do not have to manually un-load and re-load pl...

Ribonucleic acid, transfer from baker's yeast (S. cerevisiae) from Sigma-Aldrich

Preparation Note: Prepared by a modification of the procedure of Hancher, C.W., et al., Biotechnol. Bioeng., 11 , 1055 (1969). Unit Definition: One unit will yield an A 260 of 1.0 in 1.0 ml of water (1 cm light path). Physical Form: lyophilized powder Type: Type X-SA...

Dulbecco's Modified Eagle's Medium - high glucose (DME) from Sigma-Aldrich

...nce the original formulation. The most widely used modification is Dulbecco's Modified Eagle'sMedium. DME is a modification of Minimum Essential Medium Eagle [MEM] that contains a higher concentration of vitamins and amino a...

Nutrient Mixture F-12 Ham, Coon's Modification (Ham's F-12 Coon's) from Sigma-Aldrich

...brid cells that were produced by viral fusion. The modification consists of doubling the amino acids and pyruvate, and including ascorbic acid. The salt concentrations have been altered as well. This formula contains 0.863 mg zinc sulfate per liter, which may render it unsuitable for culturing mouse L-cells....

Mouse Anti-PRMT5 Monoclonal Antibody, Unconjugated, Clone PRMT5-21 from GeneTex

... methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fund...

Helix aspersa (garden snail) Lectin from Sigma-Aldrich

...t 25 C. Preparation Note: Affinity purified by a modification of the method of Hammerstrom, S. and Kabat, E.A., Biochemistry, 8 , 2969 (1969). Physical Form: lyophilized powder...

Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

...A esters.1 The EnzChek PyrophosphateAssay Kit is a modification of our EnzChek Phosphate Assay Kit(E-6646), which is based on the method originally described byWebb.2 In the presence of inorganic phosphate (Pi ), the substrate2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) isconverted enzymatically by pur...

CpGenome™ Fast DNA Modification Kit from CHEMICON

... contains reagents required to perform a bisulfite modification on a DNA sample. In the bisulfite reaction, all unmethylated cytosines are deaminated and sulfonated, converting them to uracils, while 5-methylcytosines remain unaltered. Thus, the sequence of the treated DNA will differ depending on whether the DNA...

SuperScript II RNase H- Reverse Transcriptase from Invitrogen

...maintain full polymerase activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript™ II RT superior performance characteristics....

SuperScript II RNase H- Reverse Transcriptase from Invitrogen

...maintain full polymerase activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript™ II RT superior performance characteristics....

Antisense oligonucleotides from Thermo Scientific

...phate backbone is replaced by a sulphur atom. This modification stabilises the oligonucleotide against nuclease degradation. PTOs are available as Full-PTOs All internucleotide linkages are phosphothioates Oligonucleotides with thio-caps Several internucleotide linkages at the 3 and/or 5 end are synthesise...

CpG WIZ® DAP-KINASE AMPLIFICATION KIT from CHEMICON

... However, the samples must first undergo bisulfite modification prior to PCR amplification. The CpGenome™ DN...ts necessary to perform the modification. Chemical modification creates the sequence differences between the methylated and unmethylated DNA. The primer sets in the...

ATTO-TAG FQ Amine-Derivatization Kit from Molecular Probes (Invitrogen)

...equired to obtain adequate kinetics and sufficient modification of the analyte. A recently described technique employs ATTOTAG FQ for the solid-phase derivatization of dilute solutions (10-8 M) of peptides that have been immobilized on Immobilon-CD membranes. This method permits the quantitative derivatization an...

ATTO-TAG CBQCA derivatization reagent (CBQCA; 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde) from Molecular Probes (Invitrogen)

...equired to obtain adequate kinetics and sufficient modification of the analyte. A recently described technique employs ATTOTAG FQ for the solid-phase derivatization of dilute solutions (10-8 M) of peptides that have been immobilized on Immobilon-CD membranes. This method permits the quantitative derivatization an...

ATTO-TAG CBQCA Amine-Derivatization Kit from Molecular Probes (Invitrogen)

...equired to obtain adequate kinetics and sufficient modification of the analyte. A recently described technique employs ATTOTAG FQ for the solid-phase derivatization of dilute solutions (10-8 M) of peptides that have been immobilized on Immobilon-CD membranes. This method permits the quantitative derivatization an...

Ribonucleic acid, transfer from baker's yeast (S. cerevisiae) from Sigma-Aldrich

Preparation Note: by a modification of the procedure of C.W. Hancher et al., Biotechnol. Bioeng. 11, 1055 (1969) Assay: ~ 20 A 260 /mg Solubility: water 10 mg/mL, clear, light-yellow Suitab tst: amino acid incorporation in accordance...

SoftLinx from Hudson Control Group, Inc.

... for lab personnel, yet flexible enough for custom modification by programmers. • Users create methods by building a simple drag-anddrop flowchart. If you can build a flowchart, you can write a SoftLinx method. Multitasking executable program with built-in dynamic scheduling Connectivity to >100 la...

TypeOne Restriction Inhibitor from EPICENTRE Biotechnologies

...due to the presence of one or more restriction and modification (R-M) systems that cleave unmodified DNA that is recognized as "foreign". TypeOne™ Restriction Inhibitor provides a powerful method for increasing transformation efficiencies in bacterial strains with type I R-M systems. Developed as a unique p...

TNM-FH Insect Medium (Modified Grace's Medium) from Sigma-Aldrich

...looper,Tricoplusia ni. This formula represents the modification known as TNM-FH. The medium when properly supplemented has been found to support the growth of cells derived from a variety of lepidopteran species. Sterile, Insect cell culture tested...

Mouse Anti-p14 ARF / p16Beta Ab-2 Mouse Monoclonal Antibody, Unconjugated, Clone 14P02 from Lab Vision

...rapid degradation of mdm2 proteinrequired for mdm2 modification and concurrent p53 stabilization and accumulation. This interaction is mediated by the exon 1Beta-encoded N-terminal domain of p14^ARF^ and a C-terminal region of mdm2. Deletion of the ARF-INK4a locus simultaneously impairs both the INK4a-cyclin D/...

Glycoprotein Isolation Kit WGA from Pierce Biotechnology, Inc.

Glycosylation is a post-translational modification that plays an important role in biological functions, including immune regulation, inflammation, cell-to-cell adhesion, and cell signaling. Pierce Biotechnology has developed two lectin based Glycoprotein Isolation Kits, Concanavalin A (ConA) and Whe...

Mouse Anti-Porcine Pyruvate Dehydrogenase (PDH), complex Immunocapture Kit Monoclonal Antibody, Protein G-Agarose Conjugated, Clone 15D3G9C11 from MitoScience LLC

...he assembly state, activity and post-translational modification of the complex. Pyruvate dehydrogenase is a key enzyme in energy metabolism. It converts pyruvate that has been transported into the mitochondrion into the 2-carbon molecule acetyl-CoA for subsequent breakdown in the Krebs cycle (citric acid cycle). ...

Grace's Insect Medium from Sigma-Aldrich

...peror gum moth,Antherea eucalypti. The medium is a modification of Wyatt's medium to more closely resembleAnthereahemolymph. The cell lines established by Grace using this medium were the first continuous lines developed. The basal medium, when properly supplemented, has been used to culture cells derived from a ...

SilverQuest Silver Staining Kit from Invitrogen

...l silver staining sensitivity with minimal protein modification for optimal mass spectrometry results. The mass spectrometry problems associated with traditional silver stains are eliminated with the SilverQuest kit because the reagents used do not modify protein side chains and do not interfere with trypsin dige...

Antibody Modification Services from Genescript

...ification Services GenScript offers many antibody modification options, including antibody labeling and antibody ...fragment of the antibody. GenScript's high-quality modification services rarely fail to give our clients better results in experiments and other downstream applicat...

Custom Monoclonal Antibody Development from A&G Pharmaceutical, Inc.

...lopment to targets including proteins and to their modification sites, small molecules, DNA, and cancer-specific surface antigens. A&Gs service is divided into two phases and is completed in approximately 40 days (duration may vary slightly dependent on the nature of antigens). Phase I: Immunization, test bleed...

CopyControl cDNA, Gene and PCR Cloning Kit w/ Chemically Competent E. coli cells from EPICENTRE Biotechnologies

... without the need for gel purification or post-PCR modification (e.g. addition of 3'-dA). Kits ensure >95% recombinant (white) colonies and yield > 5 X 105 cfu/µg of PCR product. Kits include chemical competent TransforMax™ EPI300™ E. coli required for induction of clones to multiple copy n...