insect

pTriEx™-4 DNA from Novagen

...asmid can be used to test expression in E. coli , insect and vertebrate cells. Transient vertebrate express... early enhancer and promoter 2 . For expression in insect cells, pTriEx-4 contains flanking baculovirus sequences to permit the generation of recombinant bacu...

TransIT-siQUEST Transfection Reagent from Mirus Bio Corporation

...ference in a diverse group of organisms as well as insect cells in culture. RNA interference leads to the inhibition of protein expression by utilizing sequence-specific, dsRNA-mediated destruction of the target messenger RNA (mRNA). Attempts to induce RNA interference using long dsRNA in mammalian cell lin...

TransIT-siQUEST Transfection Reagent from Mirus Bio Corporation

...ference in a diverse group of organisms as well as insect cells in culture. RNA interference leads to the inhibition of protein expression by utilizing sequence-specific, dsRNA-mediated destruction of the target messenger RNA (mRNA). Attempts to induce RNA interference using long dsRNA in mammalian cell lin...

BacMagic™ DNA Kit from Novagen

... preparation for recombinant protein expression in insect cells. The kit improves on the traditional method ... prevents nonrecombinant virus from replicating in insect cells, yet allows the viral DNA to be propagated as a circular DNA in bacterial cells. The target co...

Sf21 Cells (SFM Adapted) from Invitrogen

...from Spodoptera frugiperda (Fall Armyworm) ovarian insect cells (1). The Sf21 cells are adapted to serum-free suspension culture in Sf-900 II SFM for transient or stable expression of recombinant proteins....

Baculovirus Expression System with Gateway Technology from Invitrogen

...NA is then used for transfection and expression in insect cells. The Baculovirus Expression System with Gateway™ Technology offers:...

GIBCO Grace's Insect Medium Unsupplemented (1X) liquid from Invitrogen

...ce's Insect Medium is formuatled for the growth of insect cell lines including Sf9 and Sf21 cells. Grace's Insect Mediu, Unsupplemented is ideal for transfectioin because it does not contain lactalbumin hydrosylate, and yeastolate, which can interfere with transfection. Grace's Insect Medium, Supplemented i...

High Five Cells SFM Adapted from Invitrogen

...of secreted recombinant proteins compared to other insect cells. High Five cells offer the following features:- -Rapid doubling time as adherent cultures- Quick adaptation into serum-free medium- Up to 25-fold higher expression of secreted proteins than Sf9 cells- Adaptation to growth in suspension culture...

Schneider's Drosophila Medium (1X) liquid from Invitrogen

Modified and insect cell culture tested....

4% Agarose Gel from Invitrogen

...plaque assays which allow optimal growth of plated insect cells....

Baculovirus system service from Abgent

... in baculoviral expression vector, transfection of insect cells, isolation and plaque purification of ten cl...e, protein expression and tag purification from 1L insect cell culture. Expected yield and timeline: 1-4 mg of protein/8 weeks....

Custom Protein Cloning & Expression Services (Insect) from Proteome Resources

...g expected yield of purified protein, expressed in insect cells - excludes cDNA...

BacPlaque Agarose from Novagen

...oses either contain contaminants that are toxic to insect cells or lack the physical characteristics required to obtain good overlays. Novagen's BacPlaque Agarose is pre-qualified in plaque assays and transfections to ensure consistent performance....

PhosphoSafe Extraction Buffer from Novagen

...ent extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. The reagent, an enhancement of the CytoBuster Protein Extraction reagent, includes four phophatase inhibitors. PhosphoSafe is compatible with Western blotting, immunoprecipitations, and kinase assay...

Anti-G Protein Gq / 11a Subunit, Internal Sequence, a.a. 115-133 Polyclonal Antibody, Unconjugated from CHEMICON

...asma membranes and Gq/11alpha expressed in the Sf9 insect cell expression system. Negative for recombinant G(I)alpha subtypes....