Yeast Protein Kit from Zymo Research
...ble to yeast lytic enzyme digestion. The Zymolyase digestion procedure effectively generates spheroplasts of yeast cells that are ideal for both protein and DNA analyses including Western blotting and PCR, respectively....Protein Identification Service via MALDI MS/MS from Protagen AG
... of protein spots 3. High-sensitive tryptic in-gel digestion and peptide extraction 4. Preparation of samples for MALDI-MS 5. Acquisition of PMF (Peptide Mass Fingerprint) spectra via MALDI-MS (Bruker ultraflex TOF/TOF) 6. Acquisition of PFF (Peptide Fragmentation Fingerprint) spectra via MALDI-MS/MS (Bruker u...GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
...ecipitated using ammonium acetate/ethanol. The gel digestion products are alcohol soluble....GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
...ecipitated using ammonium acetate/ethanol. The gel digestion products are alcohol soluble....GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
...ecipitated using ammonium acetate/ethanol. The gel digestion products are alcohol soluble....GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
...ecipitated using ammonium acetate/ethanol. The gel digestion products are alcohol soluble....GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
...ecipitated using ammonium acetate/ethanol. The gel digestion products are alcohol soluble.......- 20 un/l Ligation-Redigestion: After 10-fold over digestion with this enzyme, the DNA fragments are ligated with T4 DNA Ligase and re-digested with this enzyme. Includes (1 ml each): 10X Bgl I Buffer and 10X Loading Buffer. A USB Floatboat will be sent free with every USB Restriction Enzyme.......- 60 un/l Ligation-Redigestion: After 10-fold over digestion with this enzyme, the DNA fragments are ligated with T4 DNA Ligase and re-digested with this enzyme. Includes (1 ml each): 10X High Buffer and 10X Loading Buffer. A USB Floatboat will be sent free with every USB Restriction Enzyme....Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
... This substrateis efficiently degraded by amylase; digestion relieves the quenchingand yields highly fluorescent fragments. The accompanyingincrease in fluorescence is proportional to amylase activity(Figures 2 and 3) and can be monitored with a fluorescencemicroplate reader or fluorometer, using standard fluo...Xcise automated robotic system for Gel Processing from Shimadzu Biotech
...ution gel imaging, precision spot cutting, protein digestion and peptide purification, and deposition onto a MALDI plate into a single integrated benchtop instrument. The Xcise is the first commercial offering from the collaboration between Shimadzu Biotech and Proteome Systems. It has undergone Shimadzus str...Chemical Inkjet Printer from Shimadzu Biotech
...earch. For the first time, micro-scale on-membrane digestion can be rapidly generated without the need for time... to the Protein Supports micro-scale on-membrane digestion of protein using pico to nanoliter volumes of reagents delivered to PVDF electroblots from 2-D gels ......emistry (IHC) applications Proteomics Protein digestion and MALDI spotting Protein crystallization and crystallization set-up Protein solubility Recombinant protein purification Drug Discovery Compound synthesis Compound weighing and dilution High throughput screening (HTS), ultrahigh throu...GenElute Minus EtBr Spin Column from Sigma-Aldrich
... There is no melting, electroelution, or enzymatic digestion of the agarose gel. More than 95% of the ethidium bromide is typically removed from the agarose....GenElute Agarose Spin Columns from Sigma-Aldrich
... There is no melting, electroelution, or enzymatic digestion of the agarose gel. Typical recovery is 40 to 45% for 100 bp to 10 kb DNA fragments. Recovery decreases with increasing fragment size. Optimal recovery can be obtained with gel slices weighing less than 100 mg. A 40 mg gel slice from a 1.0% gel will...Pyrex brand baffled trypsinizing flask, with beaded neck from Sigma-Aldrich
...homogenous tissue samples into cell suspensions by digestion of connective proteins. Heavy beaded neck accepts cotton plugs. Four extra-deep baffles enhance vigorous agitation. • Height of Baffles mm : 125...Director Universal PCR System from Sigma-Aldrich
... simple three step procedure (PCR, Exonuclease III digestion and rapid ligation/transformation) can be completed in one day. The typical cloning efficiency using this method is greater than 80%. Features and Benefits: Universal - PCR amplicon can be cloned into any expression vector High Cloning Efficiency...GPS -M Mutagenesis System from New England Biolabs
...le reaction products, thedonor can be destroyed by digestion with the rare-cutting enzyme PI-Sce I (VDE). For applications in which the mutagenized DNA will be transformed into naturally-competent organisms (which take up single DNA strands), a protocol is supplied for filling-in and ligating the gaps left by ......amino acids and peptides produced by the enzymatic digestion of casein. It is recommended for laboratory media and fermentations and has been used in antibiotics, toxins and enzyme productions. From casein, pancreatic digest For microbiology pH6.8 0.5 at 25 C, 2% in water...ProteoExtract Protein Precipitation Kit from Calbiochem
...electric focusing (IEF), 2DGE, 1DGE or for tryptic digestion prior to mass spectrometry and peptide separation schemes. • The ProteoExtract™ Protein Precipitation Kit can be used with virtually any aqueous protein sample containing between 50 mg - 10 mg / ml protein. Sample recovery is consistently ...MS Grade Protein Standards from Protea Biosciences, Inc.
...rds also provide an excellent sample for enzymatic digestion studies and characterization of LC-MS analysis systems....Topoisomerase I (Vaccinia) from Molecular Cloning Laboratories (MCLAB)
...ne base-pair Increasing restriction endonuclease digestion of resistant DNA substrates by "unwinding" the DNA coils to expose restriction sites...Plant DNAzol Reagent from Invitrogen
... Reagents can be used for restriction endonuclease digestion Southern blot analysis molecular cloning and PCR (12)....MEGAscript RNAi Kit from Ambion
... and includes reagents for transcription, nuclease digestion and clean up....Gelzyme enzyme from Invitrogen
...perature agarose gels. It is provided with Gelzyme digestion buffer....RNase III, E. coli from EPICENTRE Biotechnologies
...ragments that have 2-base, 3'-overhangs. Complete digestion of dsRNA results in dsRNA fragments of 12-15 bp. RNase III can digest double-stranded RNA composed of canonical ribonucleotides as well as DuraScript RNA, produced using EPICENTRE's DuraScribe T7 Ttranscription Kit, which contains 2'-flu...PT ModuleTM Deparaffinization and Heat-Induced Epitope Retrieval Solution (100X) from Lab Vision
...xation can be reversed successfully with enzymatic digestion for some markers while not for others. Non-enzymatic epitope unmasking techniques have been recently introduced to improve the immunoreactivity of many antigens in formaldehyde fixed tissues. Heat-Induced Epitope Retrieval (HIER) in EDTA buffer has ...PT ModuleTM Deparaffinization and Heat-Induced Epitope Retrieval Solution (10X) from Lab Vision
...xation can be reversed successfully with enzymatic digestion for some markers while not for others. Non-enzymatic epitope unmasking techniques have been recently introduced to improve the immunoreactivity of many antigens in formaldehyde fixed tissues. Heat-Induced Epitope Retrieval (HIER) in EDTA buffer has ...