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Digestion in Biological Products

Chemical Inkjet Printer from Shimadzu Biotech

Description:... micro-scale protein research. For the first time, micro-scale on-membrane digestion can be rapidly generated without the need for time consuming procedures of ... Bringing Chemistry to the Protein Supports micro-scale on-membrane digestion of protein using pico to nanoliter volumes of reagents delivered to PVDF ...
Company:Shimadzu Biotech

PT ModuleTM Deparaffinization and Heat-Induced Epitope Retrieval Solution (10X) from Lab Vision

Description:... the immunoreactivity of many antigens and epitopes. The negative effect of formaldehyde fixation can be reversed successfully with enzymatic digestion for some markers while not for others. Non-enzymatic epitope unmasking techniques have been recently introduced to improve the immunoreactivity of ...
Company:Lab Vision

PT ModuleTM Deparaffinization and Heat-Induced Epitope Retrieval Solution (100X) from Lab Vision

Description:... the immunoreactivity of many antigens and epitopes. The negative effect of formaldehyde fixation can be reversed successfully with enzymatic digestion for some markers while not for others. Non-enzymatic epitope unmasking techniques have been recently introduced to improve the immunoreactivity of ...
Company:Lab Vision

RNase III, E. coli from EPICENTRE Biotechnologies

Description:... E. coli is an endoribonuclease that specifically digests double-stranded RNA (dsRNA) to dsRNA fragments that have 2-base, 3'-overhangs. Complete digestion of dsRNA results in dsRNA fragments of 12-15 bp. RNase III can digest double-stranded RNA composed of canonical ribonucleotides as well as ...
Company:EPICENTRE Biotechnologies

Gelzyme enzyme from Invitrogen

Description:... ligations and transformations. Gelzyme enzyme is ideal for isolating DNA from low melting temperature agarose gels. It is provided with Gelzyme digestion ....
Company:Invitrogen

MEGAscript RNAi Kit from Ambion

Description:... systems. The MEGAscript RNAi Kit is based on Ambions patented high-yield transcription technol-ogy and includes reagents for transcription, nuclease digestion and clean ....
Company:Ambion

Plant DNAzol Reagent from Invitrogen

Description:... formulated for the isolation of genomic DNA from plants. Genomic DNA isolated with DNAzol Reagents can be used for restriction endonuclease digestion Southern blot analysis molecular cloning and PCR ....
Company:Invitrogen

Topoisomerase I (Vaccinia) from Molecular Cloning Laboratories (MCLAB)

Description:... of naturally occurring DNA Detecting mutant plasmids that differ in length by only one base-pair Increasing restriction endonuclease digestion of resistant DNA substrates by "unwinding" the DNA coils to expose restriction sites ...
Company:Molecular Cloning Laboratories (MCLAB)

MS Grade Protein Standards from Protea Biosciences, Inc.

Description:... each standard protein includes a high resolution mass spectrum of the protein. Our Protein Standards also provide an excellent sample for enzymatic digestion studies and characterization of LC-MS analysis ....
Company:Protea Biosciences, Inc.

ProteoExtract Protein Precipitation Kit from Calbiochem

Description:... in the buffer of choice for a wide range of proteomics downstream applications such as isoelectric focusing (IEF), 2DGE, 1DGE or for tryptic digestion prior to mass spectrometry and peptide separation schemes. • The ProteoExtract™ Protein Precipitation Kit can be used with virtually any ...
Company:Calbiochem

Peptone from Sigma-Aldrich

Description:... A high quality source of amino acids and peptides produced by the enzymatic digestion of casein. It is recommended for laboratory media and fermentations and has been used in antibiotics, toxins and enzyme productions. From casein, ...
Company:Sigma-Aldrich

GPS -M Mutagenesis System from New England Biolabs

Description:... Ampr and a pUC origin of replication. To destroy unreacted donor molecules and avoid undesirable reaction products, the donor can be destroyed by digestion with the rare-cutting enzyme PI-Sce I (VDE). For applications in which the mutagenized DNA will be transformed into naturally-competent organisms ...
Company:New England Biolabs

Director Universal PCR System from Sigma-Aldrich

Description:... Application: The simple three step procedure (PCR, Exonuclease III digestion and rapid ligation/transformation) can be completed in one day. The typical cloning efficiency using this method is greater than 80%. Features and ...
Company:Sigma-Aldrich

Pyrex brand baffled trypsinizing flask, with beaded neck from Sigma-Aldrich

Description:... These 1.5L PYREX trypsinizing flasks are used for converting homogenous tissue samples into cell suspensions by digestion of connective proteins. Heavy beaded neck accepts cotton plugs. Four extra-deep baffles enhance vigorous agitation. • Height of Baffles mm : ...
Company:Sigma-Aldrich

GenElute Agarose Spin Columns from Sigma-Aldrich

Description:... the need for silica-based resins, DEAE, or toxic organic solvents such as phenol and chloroform. There is no melting, electroelution, or enzymatic digestion of the agarose gel. Typical recovery is 40 to 45% for 100 bp to 10 kb DNA fragments. Recovery decreases with increasing fragment size. Optimal ...
Company:Sigma-Aldrich

GenElute Minus EtBr Spin Column from Sigma-Aldrich

Description:... the need for silica-based resins, DEAE, or toxic organic solvents such as phenol and chloroform. There is no melting, electroelution, or enzymatic digestion of the agarose gel. More than 95% of the ethidium bromide is typically removed from the ....
Company:Sigma-Aldrich

Freedom EVO 150 from Tecan

Description:... expression in situ hybridization (ISH), fluorescence in situ hybridization (FISH), immunohistochemistry (IHC) applications Proteomics Protein digestion and MALDI spotting Protein crystallization and crystallization set-up Protein solubility Recombinant protein purification Drug Discovery ...
Company:Tecan

Xcise automated robotic system for Gel Processing from Shimadzu Biotech

Description:... gel processing for high throughput proteome analysis. It combines the functions of high-resolution gel imaging, precision spot cutting, protein digestion and peptide purification, and deposition onto a MALDI plate into a single integrated benchtop instrument. The Xcise is the first commercial offering ...
Company:Shimadzu Biotech

Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

Description:... that is labeled with BODIPY FL dye (Figure 1) to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase ...
Company:Molecular Probes (Invitrogen)

EcoR I from USB Corp.

Description:... at 37C for 1 hour. Concentration: Standard Conc.[PN 70555]: 8 - 20 un/l High Conc.[PN 70658]: 30 - 60 un/l Ligation-Redigestion: After 10-fold over digestion with this enzyme, the DNA fragments are ligated with T4 DNA Ligase and re-digested with this enzyme. Includes (1 ml each): 10X High Buffer and 10X ...
Company:USB Corp.

Bgl I from USB Corp.

Description:... in 50 l of the reaction mixture at 37C for 1 hour. Concentration: Standard Conc.[PN 70543]: 8 - 20 un/l Ligation-Redigestion: After 10-fold over digestion with this enzyme, the DNA fragments are ligated with T4 DNA Ligase and re-digested with this enzyme. Includes (1 ml each): 10X Bgl I Buffer and 10X ...
Company:USB Corp.

GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies

Description:... small, soluble oligosaccharides. The nucleic acid can be used in the digested gel solution or precipitated using ammonium acetate/ethanol. The gel digestion products are alcohol ....
Company:EPICENTRE Biotechnologies

GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies

Description:... small, soluble oligosaccharides. The nucleic acid can be used in the digested gel solution or precipitated using ammonium acetate/ethanol. The gel digestion products are alcohol ....
Company:EPICENTRE Biotechnologies

GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies

Description:... small, soluble oligosaccharides. The nucleic acid can be used in the digested gel solution or precipitated using ammonium acetate/ethanol. The gel digestion products are alcohol ....
Company:EPICENTRE Biotechnologies

GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies

Description:... small, soluble oligosaccharides. The nucleic acid can be used in the digested gel solution or precipitated using ammonium acetate/ethanol. The gel digestion products are alcohol ....
Company:EPICENTRE Biotechnologies

GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies

Description:... small, soluble oligosaccharides. The nucleic acid can be used in the digested gel solution or precipitated using ammonium acetate/ethanol. The gel digestion products are alcohol ....
Company:EPICENTRE Biotechnologies

Protein Identification Service via MALDI MS/MS from Protagen AG

Description:... This protein identification Service includes 1. Scanning of gel(s) 2. Excision/picking of protein spots 3. High-sensitive tryptic in-gel digestion and peptide extraction 4. Preparation of samples for MALDI-MS 5. Acquisition of PMF (Peptide Mass Fingerprint) spectra via MALDI-MS (Bruker ultraflex ...
Company:Protagen AG

Yeast Protein Kit from Zymo Research

Description:... for use with S. cerevisiae and C. albicans but can be used for any fungus species that is susceptible to yeast lytic enzyme digestion. The Zymolyase digestion procedure effectively generates spheroplasts of yeast cells that are ideal for both protein and DNA analyses including Western blotting and PCR, ...
Company:Zymo Research
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