Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
...l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
...l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
...l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect......l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect......l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect......l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
...l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
...l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect...Canine Pancreatic Microsomal Membranes from Promega
...e (or ampicillin resistance gene product) from E. coli and the precursor for α-mating factor (or &...siae. • The Signal Sequence Control mRNA ( E. coli β-lactamase) is transcribed by SP6 RNA polymerase from a plasmid bearing the coding region for...pET GST Fusion System 42 plus Competent Cells from Novagen
...as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gent...pET GST Fusion System 42 from Novagen
...as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gent...pET GST Fusion System 41 plus Competent Cells from Novagen
...as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gent...pET GST Fusion System 41 from Novagen
...as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gent......l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
...l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
...l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
...l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect......l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect...Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Kidney from OriGene Technologies
...l. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well.To ensure that a clone detect...Mouse Anti-GST Tag Monoclonal Antibody, Unconjugated from Novagen
...ins containing the GSTTag sequence expressed in E. coli , yeast, mammalian, and in vitro transcription/translation systems by Western blotting, immunofluorescence or immunoprecipitation. GSTTag fusion proteins can be efficiently expressed using Novagens pET-41 or pET-42 vector series.......nant plasmid can be used to test expression in E. coli , insect and vertebrate cells. Transient vertebrat...n by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7 lac promoter. Expression can be induced in hosts such as...MagneGST™ Protein Purification System from Promega
...ansferase fusion proteins from cleared or crude E. coli cell lysate....BL21 Competent Cell Set* from Novagen
...mon method for transformation of plasmids into E. coli is to use chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility and convenience are achieved using Novagen’s prepared competent cells. Novagen offers the widest selection of...Roches RTS AviTag E. coli Biotinylation Kit, Plasmid is designed for use with high-yield RTS 100 and 500 E. coli expression kits. (Cat. No. 3 186 148, 3 186 156, 3 335 461). This kit provides vectors for the easy ...Roches RTS AviTag E. coli Biotinylation Kit, Linear Template is designed for use with high-yield RTS 100 E. coli expression kits. (Cat. No. 3 186 148, 3 186 156). The kit provides reagents to rapidly generate line...Phosphate Sensor from Invitrogen
... This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment.How it worksBinding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ~ 0.1 M), resulting in a large increase in fluorescenc...Source: E. coli strain containing an overproducing clone of T4 DNA... cofactor for the reaction. This contrasts with E. coli DNA Ligase which requires NAD. Unit Definition: One Weiss unit is the amount of enzyme required to c...DNA Polymerase I Large (Klenow) Fragment from New England Biolabs
Klenow fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3 -> 5 exonuclease activity, but has lost 5 ->3 exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5 termini....Human Topoisomerase II, Alpha from USB Corp.
Source: E. coli containing a clone of the human Topoisomerase II gene. Description: Topoisomerase II alters the topological state of nucleic acids by passing an intact DNA helix through a transient break which generates a separate DNA helix (1,2) . As a result o...M-MLV Reverse Transcriptase from USB Corp.
Source: E. coli strain containing an overproducing clone of M-MLV Reverse Transcriptase. M-MLV Reverse Transcriptase catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids(1). Full-length copies of large mRNAs, >10 kb, may be synthesize...DNA Polymerase I, E. coli from EPICENTRE Biotechnologies
DNA Polymerase I from E. coli is a DNA-dependent DNA polymerase. The enzyme also contains both 5'--3'and 3'--5' exonuclease activities.1 The 5'?3' exonuclease activity enables the enzyme to use nicks and gaps in the DNA as starting points for labeling the DNA by nick translation....Human Topoisomerase II, Alpha from USB Corp.
Source: E. coli containing a clone of the human Topoisomerase II gene. Description: Topoisomerase II alters the topological state of nucleic acids by passing an intact DNA helix through a transient break which generates a separate DNA helix (1,2) . As a result o...Source: Escherichia coli RY 13 5'G AATTC3' 3'CTTAA G5' Inactivation Conditions: Heat inactivation at 60C for 15 min or ethanol precipitation. Storage Conditions: 10mM Tris-HCl (pH 7.5), 100mM KCl, 0.1mM EDTA, 1.0mM DTT, 0.15% Triton X-100, 0.01% BSA, 50% glycerol. Shipping a...... activity (1). Novagen&'s enzyme is produced in E. coli and purified to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC 3.4.21.9, M.W. 26,300. One unit is defined as the amount of enzyme that will clea...RNase Inhibitor (Cloned) from Ambion
...itor is a recombinant human protein produced in E. coli and is a potent inhibitor of neutral pancreatic RNase A type enzymes. The mode of inhibition is noncompetitive; the inhibitor tightly binds RNases in a 1:1 ratio. The enzyme has been shown to inactivate RNases present in many tissues and cell types....RNase ONE™ Ribonuclease from Promega
...Ribonuclease is a 27kDa periplasmic enzyme from E. coli that catalyzes the degradation of RNA to cyclic nucleotide monophosphate (NMP) intermediates. Slower hydrolysis further catalyzes the degradation of these intermediates to 3-NMPs (1). RNase ONE Ribonuclease is one of the few known RNases that can cle...Klenow Fragment, Exonuclease Minus from Promega
...´ exonuclease activities present in intact E. coli DNA Polymerase I (1,2). It is used for random primer labeling (3,4) and in strand displacement amplification (5). Klenow Fragment, Exonuclease Minus, will leave a single-base 3´ overhang on a significant proportion of DNA fragments during fill-i...Rabbit Anti-M13 Genomic DNA Polyclonal Antibody, Unconjugated from Aviva Systems Biology
...ermine fusion protein expression levels both in E. coli and on the M13 virion by immunoblot analysis. Reconstitution and Storage: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 2 mg/ml. For longer periods of storage, store at -......tic activity. Novagen&'s enzyme is produced in E. coli and purified to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC 3.4.21.9, M.W. 26,300. Unit definition: one unit is defined as the amount of en...Goat Anti-SDF-1 beta / PBSF Polyclonal Antibody, Unconjugated from Sigma-Aldrich
...ecombinant human SDF-1β/PBSF expressed in E. coli ....Human MMP-14 [MT1-MMP], Catalytic Domain, E. coli recombinant from CHEMICON
...binant soluble proform of MT1-MMP purified from E. coli periplasm. SPECIFIC ACTIVITY:The specific activity of MT1-MMP catalytic domain is = 140 mU/mg, where 1 U is the activity that hydrolyzes 1 mol peptide (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-Dpa-Ala-Arg) within 1 minute under the assay condit...Mouse Anti-Human Angiogenin Monoclonal Antibody, Unconjugated, Clone 14017.7 from Sigma-Aldrich
...fied recombinant human angiogenin expressed in E. coli . Physical form: Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline. Titertest method: ELISA (c). WB. Titertest values: capture ELISA 1-2 μg/mL immunoblotting 1-2 μg/mL...FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
...to its R6K origin it can not replicate in most E. coli strains. The PCR product can therefore be used directly for downstream applications without any further purification....GPS -M Mutagenesis System from New England Biolabs
...smid can be grown easily in standard laboratory E. coli strains; the vector backbone carries Ampr and a pUC originof replication. To destroy unreacted donor molecules and avoid undesirable reaction products, thedonor can be destroyed by digestion with the rare-cutting enzyme PI-Sce I (VDE). For applicatio...Taq DNA Polymerase from ABgene
...el expression of the Taq DNA polymerase gene in E. coli Concentration: 5U/ul Applications: Standard PCR We are part of Thermo Fisher Scientific, the world leader in serving science. Thermo Fisher Scientific enables customers to make the world healthier, cleaner and safer by providing analytical instrument...E. coli Protein Sample, lyophilized, 2.7 mg from Bio-Rad
The E. coli protein sample is a complex protein sample, tested to give a consistent pattern when used with ReadyPrep 2-D starter kit rehydration and sample buffer. This sample can be used as a control to validate your 2-D system and protocol before using more di...Smac/DIABLO, Human, Recombinant, E. coli from Calbiochem
...; sequence was expressed in and purified from E. coli . Smac/DIABLO is released along with cytochrome c during apoptosis and promotes caspase activation through the neutralization of IAPs, including XIAP (Cat. No. PF137). Biological activity: IC 50 ~500-1500 nM to reverse the inhibition of XIAP (500 ...Baculovirus Expression System with Gateway Technology from Invitrogen
...-specific transposition (Tn7) in DH10Bac™ E. coli cells. The recombinant bacmid DNA is then used for transfection and expression in insect cells. The Baculovirus Expression System with Gateway™ Technology offers:...E. coli Expression System with Gateway Technology from Invitrogen
The E. coli Expression System with Gateway Technology is designed to create E. coli expression clones containing the T7 promoter. The expression clones are ready for transformation and...Ph.D.-12 Peptide 12-mer Library Kit from New England Biolabs
... (100 pmol), for automated DNA sequencing, Host E. coli strain ER2738, Control target (Streptavidin) and Elutant (Biotin), Detailed protocols for surface panning, solution panning, and ELISA characterization of selected clones...Mouse Anti-Rad 51C Monoclonal Antibody, Unconjugated, Clone 2H11/6 from ABR-Affinity BioReagents
...n: His-tagged human Rad 51C, over-expressed in E. coli Storage: 4 C......emical competent TransforMax EPI300 E. coli required for induction of clones to multiple copy number....Z-Competent E.coli Transformation Buffer Set from Zymo Research
The Z-Competent E. coli Transformation Buffer Set are convenient methods for the preparation of competent E. coli cells for simple and highly efficient DNA transformation. The Z-Competent method completely eliminat...Sheep Anti-Rabbit Tryptophan Hydroxylase Antibody, Unconjugated from ABR-Affinity BioReagents
... hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE. Storage: -20 C, Avoid Freeze/Thaw Cycles...Terrific Broth from GE Healthcare, formerly Amersham Biosciences
... extract, K2HPO4, and KH2PO4.Used at 47 g/l for E. coli growth with higher cell densities. Category: Nucleotides & Enzymes & Biochemicals, Ultrapure Biochemicals ....Alkaline Phosphatase (E. coli C75) from GE Healthcare, formerly Amersham Biosciences
Alkaline Phosphatase (E. coli C75), 100 units. Removes 5'-phosphates from DNA. Category: Nucleotides & Enzymes & Biochemicals, Modifying Enzymes, Alkaline Phosphatases....Express Cloning Checker Kit II: Green Solution from BioChain
...u need to do is to transfer partial colonies of E. coli bacteria directly from the transformation plates into the solutions provided by the kits and immediately run an agarose gel electrophoresis. Within one to two hours, you will know exactly which are recombinant colonies, instead of spending two days ...Mouse Anti-E.coli LPS Monoclonal Antibody, Unconjugated, Clone 2D7/1 from Abcam
... Antigen: Tissue / cell preparation: Escherichia coli J5 cells.......u need to do is to transfer partial colonies of E. coli bacteria directly from the transformation plates into the solutions provided by the kits and immediately run an agarose gel electrophoresis. Within one to two hours, you will know exactly which are recombinant colonies, instead of spending two days ......u need to do is to transfer partial colonies of E. coli bacteria directly from the transformation plates into the solutions provided by the kits and immediately run an agarose gel electrophoresis. Within one to two hours, you will know exactly which are recombinant colonies, instead of spending two days ...