Anti-JIP 1 / 2 (SH3) Polyclonal Antibody, Unconjugated, Clone ZMD.177 from Invitrogen
...tified MAP kinase pathways in mammals. JNK pathway activation occurs in response to environmental stress, is ass...enting its nuclear accumulation and the subsequent activation of the JNK pathway. This observation led to the original hypothesis that JIP1 acts as a cytoplasmic ...Mast Cell Degranulation colorimetric Assay Kit from CHEMICON
...ells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases. The Chemicon Mast Cell Degranulation Assay Kit provides a quick, efficient and sensitive system for evaluation of tryptase activity in cell lysates, supernatants or for inhibitor screeni...microRNA Expression Profiling - Comprehensive Service from LC Sciences
...ang, JC., Coetzee, GA., Jones, PA. (2006) Specific activation of microRNA-127 with downregulation of the protooncogene BCL6 by chromatin-modifying drugs in human cancer cells. Cancer Cell 9(6), 435-443. (3) Cummins, J.M., He, Y., Leary, R.J., Pagliarini, R., Diaz, L.A., Jr., Sjoblom, T., Barad, O., Bentwich,...ABsolute QRT-PCR SYBR Green Fluorescein Mix from ABgene
...f QRT-PCR. Enzyme is fully activated by a 15minute activation at 95C. Simple protocol Easily adapted for automation This mix with Fluorescein included is ideal for use in Bio-Rad instruments - other formats available on request Applications: real-time QRT-PCR SYBR Green I assays, end-point analysis We are part ...Factor B-depleted serum from Quidel
... Depleted sera have been used to assess complement activation in non-human species. A copy of the monograph "Quantitation of C3 activity in Murine Sera by Hemolytic Assay "is available from Quidel's technical service department....Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
...n by the enzymeadenylate cyclase and the enzymatic activation of fatty acids toform their coenzyme A esters.1 The EnzChek PyrophosphateAssay Kit is a modification of our EnzChek Phosphate Assay Kit(E-6646), which is based on the method originally described byWebb.2 In the presence of inorganic phosphate (Pi ), ...AmpliTaq Gold DNA Polymerase from Applied Biosystems
...ures, the chemical moiety is cleaved during a heat activation step, releasing active AmpliTaq® DNA enzyme. ...e" manner. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and in...GeneChoice TEMPase II DNA Polymerase from GeneChoice, Inc.
...tomated hot start amplification due to its thermal activation at a temperature well above optimal annealing. The...s optimal annealing temperatures after a 15 minute activation step at 95C, the enzyme stays activated in the reaction. Since the enzyme is chemically modified fo...Human MMP-14 [MT1-MMP], Catalytic Domain, E. coli recombinant from CHEMICON
...yr89 Gly265 of mature human MT1-MMP. Produced by activation of a recombinant soluble proform of MT1-MMP purified from E. coli periplasm. SPECIFIC ACTIVITY:The specific activity of MT1-MMP catalytic domain is = 140 mU/mg, where 1 U is the activity that hydrolyzes 1 mol peptide (7-methoxycoumarin-4-yl)acetyl-P......nes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors. Vitamin D modulates calc...FAM FLISP™ Spacer-Leu-CMK Assay Kit from B-Bridge International
...=530 nm) • Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine proteases (FLISPs) to measure chymotrypsin-like activity in whole living cells. Select from several cell-per...FAM FLISP™ Leu-DAP Assay Kit from B-Bridge International
...=530 nm) • Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine proteases (FLISPs) to measure chymotrypsin-like activity in whole living cells. Select from several cell-per...SR-101 FLISP FLISP Leu-CMK Assay Kit from B-Bridge International
...=620 nm) • Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine proteases (FLISPs) to measure chymotrypsin-like activity in whole living cells. Select from several cell-per...FAM FLISP™ Lys-CMK Assay Kit from B-Bridge International
...=620 nm) • Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine proteases (FLISPs) to measure chymotrypsin-like activity in whole living cells. Select from several cell-per...Rabbit Anti-JNK1, phospho (Thr183 / Tyr185) Polyclonal Antibody, Unconjugated from GeneTex
...erved threonine and tyrosine residue for enzymatic activation and both must be phosphorylated for full enzymatic activation. The closely related ERK1 (44 kDa) and ERK2 (42 kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif. While JNK1 is activated by dual pho...EasyCyte Plus System from Guava Technologies
... phenotyping, cell signaling, cytokine production, activation marker analysis, and multiplex compound screening. Monitoring the interplay of up to four different biological mechanisms simultaneously improves the efficiency of experimental outputs. Not only does this shorten the number of iterations for any on...Apoptosis Pathway SpliceArrays from ExonHit Therapeutics Inc.
...e variants have been reported that affect both the activation and inhibition of defined apoptotic pathways. With Apoptosis and Cell Death SpliceArrays you can perform a classical expression profiling and at the same time detect the different alternatively spliced forms of the gene that are expressed in the sam...B-Phycoerythrin Labeling Kit-NH2 from Abnova Corporation
... amino groups of IgG or other proteins without any activation process. Filtration Tube included in this kit is used for removing small molecules in the sample protein such as Tris buffer and amine compounds that interfere with the assay or labeling reaction. The maximum excitation and emission wavelengths of th......ovides a simple and convenient means for detecting activation of caspases by flow cytometry in intact cells. The assay utilizes a substrate that contains two aspartate residues linked to rhodamine 110 (D2R), a reported substrate for members of capase family proteases. The rhodamine 110 conjugated substrate is...Imject Maleimide Conjugation Buffer from Pierce Biotechnology, Inc.
...heterobifunctional cross-linker (Sulfo-SMCC). This activation results in a stable, sulfhydryl-reactive carrier protein. Thus allowing the maleimide-activated carrier protein to conjugate with cysteine residues on any peptide or protein, making a complete immunogen. Save timeno need to preactivate the carrier ...SureLINK HRP Conjugation Kit from KPL
...vated HRP is supplied ready-to-use and requires no activation or purification. Sufficient reagents are provided to conjugate 2 samples, each containing between 0.05 - 0.1 mg of antibody or protein....A23187, Mixed Calcium-Magnesium Salt from Calbiochem
...stalline solid. PROTECT FROM LIGHT. Useful in cell activation experiments when calcium dose-response data are not required. Purity: ≥98% by TLC. Soluble in DMSO or EtOH. For use in aqueous systems, preparation of a 10 mM stock in DMSO is recommended. Microliter amounts of this solution can be added to H 2...Smac/DIABLO, Human, Recombinant, E. coli from Calbiochem
...tochrome c during apoptosis and promotes caspase activation through the neutralization of IAPs, including XIAP (Cat. No. PF137). Biological activity: IC 50 ~500-1500 nM to reverse the inhibition of XIAP (500 nM) on Z-DEVD-AFC (Cat. No. 264150) cleavage in activated cell extracts. Purity: ≥95% by SDS-PAG...Dynabeads M-280 Tosylactivated from Dynal Biotech
...p-toluene-sulfonyl (tosyl) groups, further surface activation is not required. Allows easy coupling of antibodies with optimal orientation for affinity purification of proteins. Covalent coupling to primary amino- or sulphydryl groups in proteins/peptides. Coupling overnight at temperatures up to 37C and pH 8-9...The Hybrid Hunter Yeast Two-Hybrid System uses the activation of reporter genes to indicate positive protein-protein interactions. Two reporters are used an auxotrophic marker and lacZ....EGY48/pSH18-34 from Invitrogen
The Hybrid Hunter Yeast Two-Hybrid System uses the activation of reporter genes to indicate positive protein-protein interactions. Two reporters are used an auxotrophic marker and lacZ....Mouse Anti-JAB1 (Mid) Monoclonal Antibody,, Clone 3F10B10 from Invitrogen
...tide derived from the internal region of JAB1 (Jun activation domain-binding protein 1 CSN5). Specificity: Specific for the JAB1 protein. Reactivity: Human (positive controls: 293T Jurkat cell lysates). Applications: Western blotting Immunoprecipitation ELISA...CaspSCREEN Caspase Screening Kit from BioVision
Detects caspase activation by flow cytometry....Rat Plasmin from Pel-Freez Biologicals
Prepared from plasminogen by activation with immobilized human uPA...Rabbit Protein Phosphatase 1 (PP1) from Upstate
...Purification: DEAE chromatography, divalent cation activation plus trypsin digestion, and gel filtration chromatography Quality Assurance: routinely evaluated by dephosphorylation of proteins <br<Source: Purified from rabbit skeletal muscle...Molecular Immunology from Promega
...or genes; T-Lymphocyte signal transduction; B-Cell activation antibody engineering and the complement system and other immunological control mechanisms....Mouse Anti-Factor V Heavy Chain Monoclonal Antibody, Unconjugated from GeneTex
...r V. Specificity: This antibody reacts with the activation C peptide (mw ~150,000) in the heavy chain of thrombin-cleaved human Factor V. KD = 1.15 x 10 -10 mol/L....