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Translation in Biological Products

Rabbit Anti-Human PHAS-II Polyclonal Antibody, Unconjugated from Lab Vision

Description:... PHAS-II (eIF4E-binding protein II) is involved in translation initiation. PHAS-II is highest in liver and ... eIF-4E, the mRNA cap-binding protein, and inhibit translation of capped mRNA in vitro and in cells. ... the amount of the eIF-4F complex and the rate of translation initiation. Pharmacological and genetic evidence ...
Company:Lab Vision

Nick Translation System from Invitrogen

Description:... The Nick translation System is ideal for both radioactive and nonradioactive labeling of DNA. The Nick translation System: Provides five pre-mixed nucleotide solutions for flexibility in ...
Company:Invitrogen

TNT SP6 Coupled Reticulocyte Lysate System, Trial Size from Promega

Description:... simplify the process and reduce the time required to obtain in vitro translation results. Standard rabbit reticulocyte lysate translations (1) commonly use ... bypass many of these steps by incorporating transcription directly in the translation ....
Company:Promega

Transcend tRNA from Promega

Description:... The Transcend Non-Radioactive translation Detection Systems allow non-radioactive detection of proteins synthesized ... or other radioactive amino acids. This biotinylated lysine is added to the translation reaction as a precharged epsilon-labeled biotinylated lysine-tRNA complex ...
Company:Promega

ActivePro™ In Vitro Translation Kit from Ambion

Description:... Ambion's ActivePro In Vitro translation Kit, derived from an E.coli lysate, synthesizes higher amounts of protein as well as proteins with higher biological activity than competing kits. ...
Company:Ambion

7.5 M LiCl Precipitation Solution from Ambion

Description:... other RNA precipitation methods in that it does not precipitate DNA, protein or carbohydrates. It is the method of choice for removing inhibitors of translation or cDNA synthesis from RNA preparations. Note, LiCl precipitation may not remove all DNA contamination from total RNA isolations. If the RNA is to be ...
Company:Ambion

ScriptCap 2'-O-Methyltransferase from EPICENTRE Biotechnologies

Description:... The ScriptCap™ 2'-O-Methyltransferase converts Cap 0 (or Cap 0-like) RNA into Cap 1 mRNA. The Cap 1 structure has been shown to increase in vivo translation efficiency by up to 50%. This improvement is seen in Cap 0 structures created by the ScriptCap™ m7G Capping System as well as those Cap 0 ...
Company:EPICENTRE Biotechnologies

mScript mRNA Production System from EPICENTRE Biotechnologies

Description:... Create 100% capped transcripts with the translation boosting Cap 1 structure. mScript™ offers higher yields of 100% capped (versus 40% to 80% capped using co-transcriptional systems) eukaryotic ...
Company:EPICENTRE Biotechnologies

Puromycin, Dihydrochloride from Calbiochem

Description:... 2HCl White to off-white solid. PROTECT FROM MOISTURE. Protein synthesis inhibitor. Inhibits translation by causing premature release of nascent polypeptide chains. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Purity: ...
Company:Calbiochem

pCITE-2a(+) DNA from Novagen

Description:... The pCITE-2a-c(+) vectors are designed for enhanced translation of cloned sequences in vitro using mammalian cell extracts, e.g. rabbit reticulocyte lysate. The maps for pCITE-2b(+) and pCITE-2c(+) are the same as ...
Company:Novagen

LIC Duet™ Trx•Tag™ Ek Adaptor from Novagen

Description:... The LIC Duet™ Trx•Tag™ Ek Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon, and an N-terminal Trx•Tag coding sequence. The Trx•Tag is a 109 aa sequence that encodes thioredoxin. Fusion to this ...
Company:Novagen

LIC Duet™ GST•Tag™ Ek Adaptor from Novagen

Description:... The LIC Duet™ GST•Tag™ Ek Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon and an N-terminal GST•Tag coding sequence. The GST•Tag sequence has been reported to enhance the production and in some ...
Company:Novagen

LIC Duet™ T7•Tag® Ek Adaptor from Novagen

Description:... The LIC Duet™ T7•Tag ® Ek Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon, and an N-terminal T7•Tag coding sequence. The T7•Tag is a small epitope tag (11 aa) that is useful for detection of the ...
Company:Novagen

LIC Duet™ Minimal Adaptor from Novagen

Description:... The LIC Duet™ Mini Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), and an ATG translation initation codon. This adaptor is designed for expression of a target protein with minimal vector-encoded fusion ....
Company:Novagen

Taq DNA Polymerase -Klein Taq from AppliChem GmbH

Description:... of Taq DNA polymerase. It is a 5'-exo-minus N-terminally truncated Thermus aquaticus DNA polymerase. Expressed from a gene construct in E. coli, translation initiates at Met236, bypassing the 5'-3' exonuclease domain of the DNA polymerase-encoding gene. The enzyme reveals a highly active and even more ...
Company:AppliChem GmbH

m7G(5)ppp(5)G (Cap Analog) from Ambion

Description:... into a large fraction of the transcripts. Capped mRNAs are generally translated more efficiently in reticulocyte lysate and wheat germ in vitro translation systems. Uncapped mRNAs are rapidly degraded after microinjection into cells. Thus, it is important to cap in vitro transcripts for microinjection ...
Company:Ambion

m7G(5)ppp(5)G (Cap Analog) from Ambion

Description:... into a large fraction of the transcripts. Capped mRNAs are generally translated more efficiently in reticulocyte lysate and wheat germ in vitro translation systems. Uncapped mRNAs are rapidly degraded after microinjection into cells. Thus, it is important to cap in vitro transcripts for microinjection ...
Company:Ambion

DNA Polymerase I from Promega

Description:... DNA replication, and also contains a 5?3 exonuclease activity, which enables the enzyme to replace nucleotides in the growing strand of DNA by nick translation (1). The enzyme, purified from recombinant E. coli, is capable of catalyzing de novo synthesis of synthetic homopolymers and provides a convenient ...
Company:Promega

IMPACT-CN System from New England Biolabs

Description:... This allows cloning the same target gene fragment into both vectors. pTYB1 and pTYB2 use ATG of the Nde I site in the multiple cloning region for translation initiation. ...
Company:New England Biolabs

Canine Pancreatic Microsomal Membranes from Promega

Description:... of proteins. Processing events such as signal peptide cleavage, membrane insertion, translocation and core glycosylation can be examined by the translation of the appropriate mRNA in vitro in the presence of these microsomal membranes. In addition, processing and glycosylation events may be studied by ...
Company:Promega
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