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Reaction in Biological Products

iCycler Thermal Cycler With 96 x 0.2 ml Reaction Module from Bio-Rad

Description:... The iCycler thermal cycler with 96 x 0.2 ml reaction module is used for nucleic acid amplification ... It is equipped with an interchangeable 96-well reaction module (15-100 microliter samples) that is ... It includes the iCycler base with 96 x 0.2 ml reaction module, in-sample temperature probe (0.2 ml tube ...
Company:Bio-Rad

General DNA Sequencing Services from Polymorphic DNA Technologies, Inc.

Description:... liquid handling robotics optimized for automated reaction setup and dye terminator removal. Polymorphic DNA ... Templates should be provided in a sequencing reaction compatible buffer. Please submit templates ... provide 2 μg of plasmid DNA per sequencing reaction requested. For PCR products, please provide 15 ...
Company:Polymorphic DNA Technologies, Inc.

Luciferase Reporter Assay Kit from MBL International

Description:... the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction. The light emission from the reaction can be recorded using a luminometer. MBLs ... than typical flash Kinetics). The luminescent reaction is then triggered by an injection of luciferin, ...
Company:MBL International

iCycler Thermal Cycler With 384-Well Reaction Module from Bio-Rad

Description:... The iCycler thermal cycler with 384-well reaction module is used for nucleic acid amplification ... It is equipped with an interchangeable, 384-well reaction module (5-20 microliter samples) and features ... It includes the iCycler base with 384-well reaction module, plates, sealing tape, power cord, quick ...
Company:Bio-Rad

beta-Agarase from USB Corp.

Description:... on dry ice. Store at -20C. Assay Conditions: reaction mixture contains 10mM Bis-Tris-HCI (pH 6.5) and ... in at least 2 volumes of 1X Beta-Agarase reaction Buffer for approximately 20 min. *Spin briefly ... at 37C. *Add an equal volume of 1X Beta-Agarase reaction Buffer. *Add one unit of Beta-Agarase for every ...
Company:USB Corp.

Human Topoisomerase II, Alpha from USB Corp.

Description:... for long-term storage. Assay Conditions: The reaction mixture contains 10mM Tris-HCl, pH 7.9, 175mM ... Topoisomerase II, Alpha and 10X Topoisomerase II reaction Buffer (PN 73592) are functionally tested in the ... (0.1 pmol) pBR322 DNA at 30C. *Mix 10X Topo II reaction Buffer 2 l Supercoiled DNA 0.11.0 pmol ...
Company:USB Corp.

Human Topoisomerase II, Alpha from USB Corp.

Description:... for long-term storage. Assay Conditions: The reaction mixture contains 10mM Tris-HCl, pH 7.9, 175mM ... Topoisomerase II, Alpha and 10X Topoisomerase II reaction Buffer (PN 73592) are functionally tested in the ... (0.1 pmol) pBR322 DNA at 30C. *Mix 10X Topo II reaction Buffer 2 l Supercoiled DNA 0.11.0 pmol ...
Company:USB Corp.

EZ-96 DNA Methylation Kit - Deep Well from Zymo Research

Description:... analysis. The kit is based on the three step reaction that takes place between cytosine and sodium ... Kits innovative in-column desulphonation reaction eliminates several precipitation steps and provides researchers with consistent reaction conditions. The kit is designed to reduce ...
Company:Zymo Research

EZ DNA Methylation Kit from Zymo Research

Description:... analysis. The kit is based on the three step reaction that takes place between cytosine and sodium ... Kits innovative in-column desulphonation reaction eliminates several precipitation steps and provides researchers with consistent reaction conditions. The kit is designed to reduce ...
Company:Zymo Research

EZ-96 DNA Methylation Kit from Zymo Research

Description:... analysis. The kit is based on the three step reaction that takes place between cytosine and sodium ... Kits innovative in-column desulphonation reaction eliminates several precipitation steps and provides researchers with consistent reaction conditions. The kit is designed to reduce ...
Company:Zymo Research

Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.

Description:... the Gel Alcohol precipitation of the sequencing reaction products is not required. Simply load the reaction onto a standard sequencing gel. Flexible Labeling ... with thermostable inorganic Pyrophosphatase reaction Buffer (concentrate) Control DNA pUC19 Control ...
Company:USB Corp.

XpressPack™ Colorimetric Amplification Detection System, required for colorimetric XpressPack™ from CHEMICON

Description:... via hybridization with a specific oligonucleotide probe immobilized to the reaction well (target specific probe-coated plate included in the XpressPack™ ... by washing. When TMB substrate is added, a blue color develops. The reaction is stopped by the addition of an acid, changing the color to yellow. This ...
Company:CHEMICON

GPS -M Mutagenesis System from New England Biolabs

Description:... immunity acts in vivo over a distance of ~190 kb.Therefore, the in vitro reaction produces a population of target DNA molecules each containing the ... replication. To destroy unreacted donor molecules and avoid undesirable reaction products, the donor can be destroyed by digestion with the rare-cutting ...
Company:New England Biolabs

GenomeLab GeXP Genetic Analysis System from Beckman Coulter

Description:... to just a few genes at a time. GenomeLab GeXP delivers more genes per reaction and more samples per run, removing bottlenecks in gene expression studies ... and cost effectively look at the expression of 20 to 30 genes in a single reaction per well. Ideal for applications working with smaller gene sets that can ...
Company:Beckman Coulter

Hyb-Seal Slide Chambers, 20 x 63 mm, pkg of 100 from Bio-Rad

Description:... The strongly adhesive chambers are affixed over a sample on a glass slide. reaction solution is added to one of two ports, and the ports are then sealed with plastic sealing dots. Dots can be removed and replaced to allow exchange of reaction fluid. These chambers enclose a 20 x 63 mm area and hold 465 microliters. ...
Company:Bio-Rad

GeneChoice TEMPase II DNA Polymerase from GeneChoice, Inc.

Description:... yield while reducing non-specific background amplification. Once the reaction reaches optimal annealing temperatures after a 15 minute activation step ... there is no possibility for biological contamination. 10X TEMPase II reaction Buffer - Combination of (NH 4 ) 2 S0 4 and KCl, 15 mM MgCl 2 , 1% Tween ...
Company:GeneChoice, Inc.

Dithio-bis(2-nitrobenzoic acid) BioChemica from AppliChem GmbH

Description:... g/mol *CAS-No.: 69-78-3 *Storage: RT When an enzyme-catalyzed reaction creates a product with a reactive grouping not present in the substrate, ... acid (DTNB, Ellman's reagent) reacts with free thiols in an exchange reaction that produces the yellow 4-nitrothiolate anion. For the assay of ...
Company:AppliChem GmbH

GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems

Description:... thereby preventing false positive results. This kit uses an enzymatic reaction analogous to the restriction-modification and excision-repair systems of ... and AmpliTaq Gold DNA polymerase, and the other components of the PCR reaction mixture remain intact during UNG treatment, providing PCR amplification ...
Company:Applied Biosystems

DNA Exonuclease III , E. coli from EPICENTRE Biotechnologies

Description:... or nick, producing stretches of single-stranded DNA. Under defined reaction conditions, DNA degradation by Exonuclease III proceeds at a uniform rate ... excision of deoxyribonucleotides by Exonuclease III is dependent upon reaction factors including temperature, ionic strength, template sequence, and ...
Company:EPICENTRE Biotechnologies

Ligase, T4 DNA from USB Corp.

Description:... 7.5 - 7.8 Optimum Temperature: 16C for exchange reaction Requirements for Divalent Cation: Mg 2+ , Mn 2+ ... exchange method of Weiss et al. The reaction mixture (300 l) contains 67mM Tris-HCl, (pH 7.8), ... 4543-4555. Functionally Tested 10X T4 DNA Ligase reaction Buffer (1 ml included, PN 70087): 660mM Tris-HCl ...
Company:USB Corp.

GeneChoice Taq DNA Polymerase With Magnesium Free Standard Buffer from GeneChoice, Inc.

Description:... of MgCl 2 levels. Each Kit contains Taq DNA Polymerase, 10X Mg Free reaction Buffer, and 25 mM MgCl 2 . Enzyme Concentration - 5 units/µL. 10X Magnesium Free reaction Buffer Components - 100 mM Tris-HCl pH 8.5, 500 mM KCl, 1% Triton ....
Company:GeneChoice, Inc.

GeneChoice Taq DNA Polymerase With Ammonium Buffer from GeneChoice, Inc.

Description:... fidelity and GC-rich templates. Each Kit contains Taq DNA Polymerase, 10X reaction Buffer, and 25 mM MgCl 2 . Enzyme Concentration - 5 units/µL. 10X Ammonium reaction Buffer Components - 750 mM Tris-HCl pH 8.5, 200 mM (NH 4 ) 2 SO 4 , 15 mM ...
Company:GeneChoice, Inc.

GeneChoice Taq DNA Polymerase With Standard Buffer from GeneChoice, Inc.

Description:... Buffer for routine templates. Each Kit contains Taq DNA Polymerase, 10X reaction Buffer, and 25 mM MgCl 2. Enzyme Concentration - 5 units/µL. 10X Standard reaction Buffer Components - 100 mM Tris-HCl pH 8.5, 500 mM KCl, 15 mM MgCl 2 , 1% ...
Company:GeneChoice, Inc.

AmpliTaq Gold DNA Polymerase from Applied Biosystems

Description:... is inactive. During set-up and the first ramp of thermal cycling (when the reaction is going through non-optimal annealing temperatures), the enzyme is inactive. The result is that mis-primed primers are not extended. Once the reaction reaches optimal annealing temperatures, the chemical moiety is cleaved ...
Company:Applied Biosystems

Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

Description:... into two equivalents of Pi. The Pi is then consumed by the MESG/PNP reaction and detected by an increase in absorbance at 360 nm. Additional ... and can be performed over a pH range of 6.5 to 8.5.2 The MESG/PNP reaction has been used for continuous spectrophotometric determination of ...
Company:Molecular Probes (Invitrogen)

Varian 820-MS from Varian, Inc.

Description:... The 820-MS features Varians unique collision reaction interface (CRI) system*, which provides simple, routine removal of ... unique* collision reaction interface (CRI) system makes setup of complex reaction cell conditions a thing of the past. Simply turn on the gas flow to remove ...
Company:Varian, Inc.

DNA Fragment 2X Precipitation Solution from EPICENTRE Biotechnologies

Description:... Enables rapid purification of double-stranded DNA fragments = 200 bp, including, for example, PCR reaction products, DNA fragments obtained in restriction enzyme digests, or double-stranded ...
Company:EPICENTRE Biotechnologies

T4 Polynucleotide Kinase Exchange Reaction Buffer from Invitrogen

Description:... For use with T4 Polynucleotide Kinase for labeling of 5' ends (5' phosphate ends) of nucleic acids. In the exchange reaction T4 Polynucleotide Kinase transfers the 5' phosphate group from DNA to ADP and subsequently transfers the Gamma-phosphate of ATP to the free 5' ...
Company:Invitrogen

5X MEGAscript® T7 Kit from Ambion

Description:... Exclusive ultra high yield technology is fast - 2 hour reaction time Amplifies aRNA for gene array analysis Efficiently incorporates many modified nucleotides Kits specific for templates with T7, T3 and ...
Company:Ambion

ReBlot Western Blot Recycling Kit - Trial from CHEMICON

Description:... stripping colorimetric substrates (TMB, DAB, 4-chloronapthol, etc.), as it is not possible to effectively remove substrates that precipitate at the reaction ....
Company:CHEMICON

Anti-Sheep/Goat Sac-Cel - Solid-phase Separation System from IDS Ltd

Description:... with primary antibody (in the absence of potentially interfering carrier serum) and tracer, Sac-Cel is added. Following a second incubation, the reaction volume is increased by dilution prior to a short, low speed spin (typically 2 minutes at just 1,000 g). This effectively washes the solid-phase ...
Company:IDS Ltd

ReBlot Plus Strong Antibody Stripping Solution from CHEMICON

Description:... stripping colorimetric substrates (TMB, DAB, 4-chloronapthol, etc.), as it is not possible to effectively remove substrates that precipitate at the reaction site. The Re-Blot Plus Western Blot Strong Antibody Stripping Solution should be used only for qualitative purposes until it has been established by ...
Company:CHEMICON

SureLINK Bioconjugation Kit from KPL

Description:... of bioconjugation such as maleimide/thiol and avidin/biotin. Using a highly selective interaction of hydrazine and carbonyl groups, the coupling reaction results in a hydrazone bond that is extremely stable. Additionally, no reducing reagents are required, removing the risk of protein denaturing and ...
Company:KPL

OLIGO from Molecular Biology Insights

Description:... OLIGO is a multi-functional program that searches for and selects oligonucleotides from a sequence file for polymerase chain reaction (PCR), DNA sequencing, site-directed mutagenesis, and various hybridization applications. It calculates hybridization temperature and secondary ...
Company:Molecular Biology Insights

XpressPack™ Luminescent Amplification Detection System, required for luminescent XpressPack™ from CHEMICON

Description:... the two DNA strands. The single-stranded amplicon is captured to the plate via hybridization with a specific oligonucleotide probe immobilized to the reaction well (target specific probe-coated plate included in the XpressPack gene-specific kit). The hybrid complex is then reacted with streptavidin ...
Company:CHEMICON

GenomeLab SNPstream Genotyping System from Beckman Coulter

Description:... up to 48 SNPs in each well of an arrayed 384-well plate, for cost-effective high throughput SNP analysis. Based on single-based primer extension reaction technology, the system integrates hardware, software and reagents in a compact design. SNPstream users can also access Beckman Coulters ...
Company:Beckman Coulter

Mts-Atf-LC-Biotin Label Transfer Reagent, 5 mg. from Pierce Biotechnology, Inc.

Description:... protein-containing lysate, the Atf moiety is activated with UV light capturing the interacting complex. Reduction of the disulfide bond formed by reaction of -SH with the Mts group of the label-transfer reagent results in the transfer of the biotin label from the bait protein to the prey protein. The ...
Company:Pierce Biotechnology, Inc.

Mts-Atf-Biotin Label Transfer Reagent, 5 mg from Pierce Biotechnology, Inc.

Description:... protein-containing lysate, the Atf moiety is activated with UV light capturing the interacting complex. Reduction of the disulfide bond formed by reaction of -SH with the Mts group of the label-transfer reagent results in the transfer of the biotin label from the bait protein to the prey protein. The ...
Company:Pierce Biotechnology, Inc.

Universal Kinase / Phosphatase Fluorescence superquenching Assay Kit, TruLight™ from Calbiochem

Description:... provides flexibility for assaying any kinase or phosphatase. User must supply the quencher-labeled peptide substrate, calibrator peptide, specific reaction buffer, ATP, 96- or 384-well plate(s), and any additional components specific to the enzyme being tested. reference: Rininsland, F., et al. 2004. ...
Company:Calbiochem

Anti-Factor XIIIa Ab-2 Polyclonal Antibody, Unconjugated from Lab Vision

Description:... heterodimer (M.W. 320kDa); whereas in platelets, only the Alpha2 unit exists. Factor XIIIa is a dermal dendrocyte marker and shows variable reaction with these types of tumors. It can be used for histiocytic phenotyping and has been reported to mark capillary hemangiomas and tumors of the central ...
Company:Lab Vision
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