ProteoExtract Protein Precipitation Kit from Calbiochem
Description:... such as 2DGE. Precipitation of proteins is a protein purification procedure that can both concentrate the proteins and remove interfering substances ... for the concentration and clean up of 200 samples of 0.2 ml volume. The procedure is scalable, samples with volumes smaller or larger than 0.2 ml can be ...Director Universal PCR System from Sigma-Aldrich
Description:... Application: The simple three step procedure (PCR, Exonuclease III digestion and rapid ligation/transformation) can be ... to 6.5Xgreater than standardTaqDNA polymerase Fast - Simple three-step procedure allows completion in less than one day ...One-Step Deparaffinization Solution from Lab Vision
Description:... the slides in a series of baths of xylene and graded alcohols. This procedure is time-consuming which takes more than 30 minutes and requires special ... and rehydration of tissue sections using a single reagent. The new procedure is easy to perform and requires less than one ....ProteoSpin Micro Detergent Clean Up Kit from Norgen Biotek Corporation
Description:... The ProteoSpin Detergent Clean-up Micro Kit provides a fast and simple procedure for the effective removal of SDS, Triton X-100 and other detergents from ... time - In contrast to longer dialysis procedures, the easy-to-use procedure allows processing up to 12 samples in only 20 ....mirVana miRNA Isolation Kit from Ambion
Description:... RNAs. Ambions mirVana miRNA Isolation Kit purifies all RNA species (including small RNAs), both single- and double-stranded. Through an enrichment procedure included in the mirVana miRNA Isolation Kit, RNA molecules less than 200 nt in length can be efficiently purified away from larger RNA species. As ...CaspGLOW Red Caspase Staining Kit from BioVision
Description:... Detects activated caspases in living cells. Simple/sensitive/straight-forward procedure takes only 30 minutes. Results can be analyzed by flow cytometry/fluorescence microscopy/fluorescence plate ....Qentix (tm) Western Blot Signal Enhancer from Pierce Biotechnology, Inc.
Description:... signal up to 10-fold (or one order of magnitude) in only 15 minutes. The Western Blot Signal Enhancer membrane treatment is a simple, 15-minute procedure that can be added to your current Western blotting protocol. The result is an increase in the intensity of target protein bands on the Western blot ...ReadyPrep 2-D Cleanup Kit from Bio-Rad
Description:... on 2-D gels by removal of detergents, phenolic compounds, salts, and most other ionic contaminants. It also concentrates dilute samples. The kit procedure involves a TCA-like precipitation. Recommended sample sources include mammalian tissue or cells, plant leaves, E. coli, and yeast. The kit includes ...MasterAmp Taq DNA Polymerase Kit from EPICENTRE Biotechnologies
Description:... exonuclease activity, but lacks 3'--5' proofreading exonuclease activity. MasterAmp Taq DNA Polymerase is highly purified using a proprietary procedure that virtually eliminates contaminating bacterial DNA. The enzyme is provided with a 10X solution of MasterAmp PCR Enhancer (with betaine). The ...ApoBrdU Red DNA Fragmentation Kit from MBL International
Description:... and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections. The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods. Results can be analyzed by flow cytometry. The kit works well with GFP ...Synaptic Vesicles Isolation Kit from Sigma-Aldrich
Description:... The Synaptic Vesicles Isolation Kit provides all the reagents required for preparing an enriched synaptic vesicle fraction. The isolation procedure involves tissue homogenization, hypo-osmotic lysis of the synaptosomes, and differential centrifugation followed by an OptiPrep density gradient. The ...SpinPrep Gel DNA Kit from Novagen
Description:... The SpinPrep Gel DNA Kit enables efficient extraction of DNA fragments from 150 bp to > 12,000 bp in size from agarose gels. The procedure uses GelMelt solution to dissolve the gel slice, followed by adsorption of the DNA to a silica membrane in a spin column format. After a wash step, ...Introductory SpinPrep Gel DNA Kit from Novagen
Description:... DNA from agarose gels The SpinPrep Gel DNA Kit enables efficient extraction of DNA fragments of 150 bp to > 12,000 bp in size from agarose gels. The procedure uses GelMelt solution to dissolve the gel slice, followed by adsorption of the DNA to a silica membrane in a spin column format. After a wash step, ...ApoBrdU DNA Fragmentation Assay Kit from MBL International
Description:... and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections. The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods. Results can be analyzed by fluorescence microscopy or flow cytometry. ...ClearSpin Filters from Novagen
Description:... lysate is centrifuged at 10,000 g for five minutes and the supernatant is decanted into a ClearSpin Filter to recover the clarified lysate. This procedure can save up to 40 minutes over standard methods and it eliminates the need for 20,000 g spins. The ClearSpin Filter fits into a standard 50-ml ...Ribonucleic acid, transfer from baker's yeast (S. cerevisiae) from Sigma-Aldrich
Description:... Preparation Note: by a modification of the procedure of C.W. Hancher et al., Biotechnol. Bioeng. 11, 1055 (1969) Assay: ~ 20 A 260 /mg Solubility: water 10 mg/mL, clear, light-yellow Suitab tst: ...LEUCOPERM Suspension fixation and permeabilization solution from GeneTex
Description:... medium LEUCOPERM reagents are intended for fixing cells in suspension with Reagent A and then permeabilising the cells with Reagent B. This procedure gives antibodies access to intracellular structures and leaves the morphological scatter characteristics of the cells intact. Specific formulations ...PAP Pen from ScyTek Laboratories
Description:... PAP Pen contains a special formulation which is insoluble in alcohol and acetone. It can be removed, if desired, by xylene after the staining procedure is ....MitoPT Detection of Mitochondrial Permeability from B-Bridge International
Description:... MitoPT kit is used in conjunction with your existing apoptosis protocols. Grow your cells in culture and induce apoptosis according to your existing procedure (also reserve a non-induced population of cells as a negative control). Once you have induced apoptosis in your cells, add the 1X MitoPT solution to ...Amphetamine ELISA Kit, Ultra from Neogen Corporation
Description:... are intended for forensic use only. It is recommended that all suspect samples be confirmed by a quantitative method such as GC/MS. The simple assay procedure includes the addition of samples or controls to the antibody-coated microplate followed by the addition of the enzyme conjugate (HRP). During an ...Human TRAP 5 ImmunoCapture Assay Kit from BioVendor Laboratory Medicine, Inc.
Description:... An immuncapture enzyme-activity assay based on measurenment of enzyme activity of TRAP 5 captured on monoclonal antibody coated microtiter plate. The procedure includes two incubation steps (1st standards, samples or controls, 2nd enzyme reaction) separated by a washing step. The assay is intended for the ...Frozen-EZ Yeast Transformation II Kit from Zymo Research
Description:... The transformation of Saccharomyces cerevisiae, as well as other yeasts, has become a routine procedure in many molecular biology laboratories. The ability to transform whole cells at high efficiencies with plasmid and linear DNAs using lithium based ...Luciferase Luminescent Assay Kit, LucLite from PerkinElmer
Description:... in microplates, load them into the microplate stacker, and analyze all samples unattended. The luclite assay system also provides the most simple procedure available - just mix two vials of reagent, dispense into the wells of a microplate containing transfected cells, seal the plate, shake briefly and ...EZ-96 DNA Methylation Kit - Deep Well from Zymo Research
Description:... The EZ DNA Methylation Kit uses a simplified procedure and streamlines the bisulfite method for DNA methylation analysis. The kit is based on the three step reaction that takes place between cytosine and ...GPDH Assay Kit from Takara Mirus Bio
Description:... repressors or to study mechanisms regulating differentiation of primary or established cell-line progenitor cells into adipocytes. The procedure involves extracting cells by addition of the Enzyme Extraction Buffer, followed by serial dilution of the extract. The serially diluted extract is ...EZ DNA Methylation Kit from Zymo Research
Description:... The EZ DNA Methylation Kit uses a simplified procedure and streamlines the bisulfite method for DNA methylation analysis. The kit is based on the three step reaction that takes place between cytosine and ...EZ-96 DNA Methylation Kit from Zymo Research
Description:... The EZ DNA Methylation Kit uses a simplified procedure and streamlines the bisulfite method for DNA methylation analysis. The kit is based on the three step reaction that takes place between cytosine and ...ProteoSpin Maxi Detergent Clean Up Kit from Norgen Biotek Corporation
Description:... The ProteoSpin Detergent Clean-up Maxi Kit provides a fast and simple procedure for the effective removal of SDS, Triton X-100 and other detergents from protein samples. The kit employs spin-column chromatography using Norgens ...ProteoSpin Urine Protein Concentration Kit from Norgen Biotek Corporation
Description:... to blood plasma as a potential source of disease biomarkers. The ProteoSpin Urine Protein Concentration Micro Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from small volumes of urine. The kit employs spin-column chromatography using Norgens ...Yeast Protein Kit from Zymo Research
Description:... S. cerevisiae and C. albicans but can be used for any fungus species that is susceptible to yeast lytic enzyme digestion. The Zymolyase digestion procedure effectively generates spheroplasts of yeast cells that are ideal for both protein and DNA analyses including Western blotting and PCR, ....ProteoSpin Micro CBED Kit from Norgen Biotek Corporation
Description:... The ProteoSpin CBED Micro Kit provides a fast and simple procedure for concentration, buffer exchange and desalting of protein samples. The kit is based on spin-column chromatography using Norgens patented protein ...ProteoSpin Maxi CBED Kit from Norgen Biotek Corporation
Description:... The ProteoSpin CBED Maxi Kit provides a fast and simple procedure for concentrating large volumes of dilute protein solutions for buffer exchange, and for removing different types of salts from protein samples. The ...EpiTect Bisulfite Kit from QIAGEN
Description:... 6 hours. The highly sensitive method utilizes an innovative protection against DNA degradation and a spin-column-based purification and desulfonation procedure to guarantee fast and reliable results for all downstream applications. The EpiTect Bisulfite Kit complements QIAGENs range of DNA cleanup and ...Ribonucleic acid, transfer from baker's yeast (S. cerevisiae) from Sigma-Aldrich
Description:... Preparation Note: Prepared by a modification of the procedure of Hancher, C.W., et al., Biotechnol. Bioeng., 11 , 1055 (1969). Unit Definition: One unit will yield an A 260 of 1.0 in 1.0 ml of water (1 cm ...