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Ligation in Biological Products

Rapid DNA Ligation Kit from Roche Applied Science

Description:... The Rapid DNA ligation Kit enables ligation of sticky-end or blunt-end DNA fragments in just ... or concatemeric (high DNA concentration) ligation products are ....
Company:Roche Applied Science

Ligase, T4 DNA from USB Corp.

Description:... One Weiss unit equals about 67 cohesive end ligation units. Concentration: PN 70005: 1 unit/l; PN ... synthetic linkers or adaptors. PROTOCOL FOR DNA ligation For each 10 l of ligation reaction, a combination of 2040 ng of vector DNA ...
Company:USB Corp.

Zero Blunt TOPO Kit for Sequencing with One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli from Invitrogen

Description:... are available. The pCR4-TOPO vector provides 3-T overhangs for direct ligation of Taq-amplified PCR products. The pCR4Blunt-TOPO vector provides blunt ... linearized and topoisomerase I-activated enabling 5-minute bench-top ligation yielding recombinants (1). Both vectors ...
Company:Invitrogen

PEG 8000 Powder from Promega

Description:... PEG 8000 is used in the precipitation of phage, isolation of plasmid DNA and the enhancement of blunt-ended ligation ....
Company:Promega

Zero Background Cloning Kit from Invitrogen

Description:... utilizes positive selection to eliminate high background. The small size of the Zeocin resistance gene reduces the overall vector size for increased ligation and transformation efficiencies. The kit contains all of the reagents you need for simple cloning and analysis of your ....
Company:Invitrogen

GFX PCR DNA and Gel Band Purification Kit from GE Healthcare, formerly Amersham Biosciences

Description:... for immediate use in most applications such as automated and manual DNA sequencing, nested PCR amplification, DNA labeling, restriction analysis, ligation and cloning. Category: DNA/RNA Synthesis & Purification, Purification of PCR Products and Restriction ....
Company:GE Healthcare, formerly Amersham Biosciences

HTP Zero Blunt TOPO PCR Cloning Kit for Sequencing MultiShot E. coli from Invitrogen

Description:... products. TOPO TA Cloning and Zero Blunt TOPO vectors are supplied linearized and activated with topoisomerase I. This enables 5-minute, bench-top ligation and yields >=95% ...
Company:Invitrogen

HTP Zero Blunt TOPO PCR Cloning Kit for Sequencing MultiShot StripWell E. coli from Invitrogen

Description:... products. TOPO TA Cloning and Zero Blunt TOPO vectors are supplied linearized and activated with topoisomerase I. This enables 5-minute, bench-top ligation and yields >=95% ...
Company:Invitrogen

TargeTron Gene Knockout System from Sigma-Aldrich

Description:... Sigma website (www.sigma-aldrich.com/targetronaccess), using one or more of the output primer sets to mutate the TargeTron intron template via PCR, ligation of the fragment into the pACD4K-C linear vector, transformation of the host, induction of gene disruption, selection by kanamycin resistance ...
Company:Sigma-Aldrich

Gel extraction kit from BioPioneer

Description:... removal of contaminants. Purified DNA can be used in any downstream application such as sequencing, labeling, restriction enzymatic digestion, ligation or transformation. * No phenol / chloroform extraction or ethanol precipitation is ....
Company:BioPioneer

T4 DNA Ligase from Invitrogen

Description:... bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation which can be performed in 5 minutes (1). Single-stranded nucleic acids are not substrates for this enzyme. A T4 DNA Ligase Technical Bulletin is ...
Company:Invitrogen

Tfi DNA Ligase, 2,000U, thermostable from Bioneer, Inc.

Description:... temperatures than conventional DNA ligases, Thus, Tfi DNA Ligase is the ideal enzyme for applications the require high-temperature, high-stringency ligation of duplex DNA ....
Company:Bioneer, Inc.

T4 DNA Ligase; (1 x 103 U/ml) or (5 x 103 U/ml) supplied with 10x ligation buffer that includes ATP from Roche Applied Science

Description: Request ...
Company:Roche Applied Science

T4 DNA Ligase; (1 x 103 U/ml) or (5 x 103 U/ml) supplied with 10x ligation buffer that includes ATP from Roche Applied Science

Description: Request ...
Company:Roche Applied Science
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