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Ligase in Biological Products

T4 DNA Ligase from Invitrogen

Description:... T4 DNA ligase catalyzes the formation of phosphodiester bonds ... and 5' phosphate termini. The unique T4 DNA ligase buffer optimizes ligation which can be performed ... are not substrates for this enzyme. A T4 DNA ligase Technical Bulletin is available ....
Company:Invitrogen

Ligase, T4 DNA from USB Corp.

Description:... clone of T4 DNA Ligase. Description: T4 DNA ligase catalyzes the formation of a phosphodiester bond ... dry ice. Store at -20C. Assay Conditions: T4 DNA ligase is assayed according to the pyrophosphate ... for the reaction. This contrasts with E. coli DNA ligase which requires NAD. Unit Definition: One Weiss ...
Company:USB Corp.

Tfi DNA Ligase, 2,000U, thermostable from Bioneer, Inc.

Description:... Tfi DNA ligase catalyzes the formation of a phosphodiester bonds ... with a complementary strand at 45 - 65?. Tfi DNA ligase is stable and active at much higher temperatures than conventional DNA ligases, Thus, Tfi DNA ligase is the ideal enzyme for applications the require ...
Company:Bioneer, Inc.

Zero Blunt TOPO PCR Cloning Kit with One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli from Invitrogen

Description:... polymerases. The kit includes the linearized and topoisomerase I-activated pCR-Blunt II-TOPO vector for 5-minute bench-top ligations without ligase (1). The pCR-Blunt II-TOPO vector also ...
Company:Invitrogen

T4 DNA Ligase from Promega

Description:... T4 DNA ligase catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a ...
Company:Promega

EcoR I from USB Corp.

Description:... High Conc.[PN 70658]: 30 - 60 un/l Ligation-Redigestion: After 10-fold over digestion with this enzyme, the DNA fragments are ligated with T4 DNA ligase and re-digested with this enzyme. Includes (1 ml each): 10X High Buffer and 10X Loading Buffer. A USB Floatboat will be sent free with every USB ...
Company:USB Corp.

Bgl I from USB Corp.

Description:... Standard Conc.[PN 70543]: 8 - 20 un/l Ligation-Redigestion: After 10-fold over digestion with this enzyme, the DNA fragments are ligated with T4 DNA ligase and re-digested with this enzyme. Includes (1 ml each): 10X Bgl I Buffer and 10X Loading Buffer. A USB Floatboat will be sent free with every USB ...
Company:USB Corp.

Rabbit Anti-Human COP9 signalosome complex subunit 2 Polyclonal Antibody, Unconjugated from Aviva Systems Biology

Description:... CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP. COPS2 is also innvolved ...
Company:Aviva Systems Biology

26S Proteasome Fraction Extract from GeneTex

Description:... activating enzyme (E1), ubiquitin conjugating enzymes (E2s), 20S and 26S proteasomes, isopeptidase, proteasome PA28/11S REG complex, and ubiquitin ligase enzymes (E3s). GTX80880 is ideal for demonstrating ubiquitin proteasome degradation/conjugation of radiolabeled or immunodetectable substrates. ...
Company:GeneTex

RTS AviTag E. coli Biotinylation Kit, Linear Template; Linear template generation and in vitro protein mono-biotinylation system for use with RTS 100 expression reactions from Roche Applied Science

Description:... protein expression reactions without the need for purification.The expressed protein can be enzymatically mono-biotinylated by E. coli biotin protein ligase (BirA, E.C. 6.3.4.15). In addition to E. coli Bir A the kit supplies a substrate energy mixture, which is required for the biotinylation ....
Company:Roche Applied Science

RTS AviTag E. coli Biotinylation Kit, Plasmid; Cloning vector and in vitro protein mono-biotinylation system for use with RTS 100 and RTS 500 E. coli HY Kits from Roche Applied Science

Description:... introduction of a C- or N-terminal AviTag peptide sequence. The expressed protein can be enzymatically mono-biotinylated by E. coli biotin protein ligase (BirA, E.C. 6.3.4.15). The vectors contain all regulatory elements necessary for expression in RTS reactions using a combination of T7 RNA polymerase ...
Company:Roche Applied Science

Baker's yeast (S. cerevisiae) S-Acetyl-coenzyme A synthetase from Sigma-Aldrich

Description:... Acetate: CoA ligase (AMP forming), Acetate thiokinase Packaging: Package size based on protein content. Physical form: Lyophilized powder containing stabilizers as ...
Company:Sigma-Aldrich

Ready-To-Go T4 DNA Ligase from GE Healthcare, formerly Amersham Biosciences

Description: ...
Company:GE Healthcare, formerly Amersham Biosciences

T4 RNA Ligase from Roche Applied Science

Description: Request ...
Company:Roche Applied Science

T4 DNA Ligase (HC) from Invitrogen

Description: Request ...
Company:Invitrogen
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