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PhageMaker System from Novagen

Description:... DNA) are typically obtained. This one-tube system is extremely easy to use; simply add up to 5 g of ligated vector plus insert to the extract and incubate at room temperature for 2 ....
Company:Novagen

MitoPT Detection of Mitochondrial Permeability from B-Bridge International

Description:... population of cells as a negative control). Once you have induced apoptosis in your cells, add the 1X MitoPT solution to each population and incubate the cells for an additional 15 minutes. During this incubation time, the MitoPT reagent will enter each cell and the mitochondria inside. If the cell ...
Company:B-Bridge International

iEMS Incubator/Shaker 3 module (100-120 V) from Thermo Scientific

Description:... performance microplate incubator and orbital shaker. Its superior temperature control and efficient orbital shaking reduce incubation times. It can incubate up to 9 ....
Company:Thermo Scientific

beta-Agarase from USB Corp.

Description:... *Add 2 volumes of TE (pH 7.6) to prevent precipitation of the oligosaccharides. Precipitate with 0.05 volumes of NaCl and 2 volumes of 95% ethanol. incubate at -20C for 30 min, then spin in a microcentrifuge. Wash the pellet twice with 70% ethanol. *The DNA solution may be used directly for ligation, ...
Company:USB Corp.

Human Topoisomerase II, Alpha from USB Corp.

Description:... DNA at 30C. *Mix 10X Topo II Reaction Buffer 2 l Supercoiled DNA 0.11.0 pmol Topoisomerase II, Alpha 110 units H 2 O to 20 l *Mix and incubate at 3037C for 15 minutes. *Stop the reaction by the addition of 3 l of 7mM EDTA or by the addition of SDS to ....
Company:USB Corp.

Human Topoisomerase II, Alpha from USB Corp.

Description:... DNA at 30C. *Mix 10X Topo II Reaction Buffer 2 l Supercoiled DNA 0.11.0 pmol Topoisomerase II, Alpha 110 units H 2 O to 20 l *Mix and incubate at 3037C for 15 minutes. *Stop the reaction by the addition of 3 l of 7mM EDTA or by the addition of SDS to ....
Company:USB Corp.

Ligase, T4 DNA from USB Corp.

Description:... ng) __ l Insert DNA (310-fold molar excess) __ l Ligation Buffer, 10X 5 l Water __ l T4 DNA Ligase __ l (1 unit) Total 50 l 2. Mix and incubate at 16C for 16 hrs. Note: K m for the activity of T4 DNA Ligase on blunt-ended DNA is nearly 100 times higher than that on DNA with cohesive ends. ...
Company:USB Corp.
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