Enzyme in Biological Products
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Enzyme in Biological Products
Gelzyme enzyme from Invitrogen
Description:... Gelzyme is an agarose-digesting enzyme isolated from Pseudomonas atlantica. Gelzyme enzyme cleaves simple carbohydrate bonds in agarose ... DNA manipulation. DNA isolated with Gelzyme enzyme can be used for restriction endonuclease ...Company:Invitrogen
Description:... of the complex. Pyruvate dehydrogenase is a key enzyme in energy metabolism. It converts pyruvate that ... described for measuring this activity while the enzyme is still bound to the capture beads or fixed in a ... diabetes (NIDMM) and the response of the enzyme complex to diet, exercise and ....
Company:MitoScience LLC
FailSafe PCR System w/choice of two PreMix Tubes from EPICENTRE Biotechnologies
Description:... This system uses EPICENTRE's FailSafe™ PCR enzyme Mix, a unique blend of thermostable enzymes that ... to determine error rates, the FailSafe enzyme Mix has a fidelity at least 3 times higher than ... The system includes the FailSafe™ PCR enzyme Mix and a choice of two FailSafe™ PreMixe ...Company:EPICENTRE Biotechnologies
FailSafe PCR PreMix Selection Kit from EPICENTRE Biotechnologies
Description:... This system uses EPICENTRE's FailSafe™ PCR enzyme Mix, a unique blend of thermostable enzymes that ... to determine error rates, the FailSafe enzyme Mix has a fidelity at least 3 times higher than ... The system includes the FailSafe™ PCR enzyme Mix, an extensively tested set of 12 ...Company:EPICENTRE Biotechnologies
MasterAmp Taq DNA Polymerase Kit from EPICENTRE Biotechnologies
Description:... above 70°C, it was the first thermostable enzyme used for PCR and remains the predominant enzyme for that purpose. The enzyme has an intrinsic 5'?3' structure-dependent ...Company:EPICENTRE Biotechnologies
FailSafe PCR System w/choice of 8 PreMix Tubes from EPICENTRE Biotechnologies
Description:... This system uses EPICENTRE's FailSafe™ PCR enzyme Mix, a unique blend of thermostable enzymes that ... to determine error rates, the FailSafe enzyme Mix has a fidelity at least 3 times higher than ... The system includes the FailSafe™ PCR enzyme Mix and a choice of 8 FailSafe™ Premix ....Company:EPICENTRE Biotechnologies
Human Caspase-7 from Calbiochem
Description:... Mch3 Lyophilized solid. Highly active enzyme useful for the study of enzyme regulation and kinetics, cleavage of target ... protein. One unit is defined as the amount of enzyme that will cleave 1 nmol of Ac-DEVD-pNA Cat. No. ...Company:Calbiochem
GeneChoice TEMPase II DNA Polymerase from GeneChoice, Inc.
Description:... a temperature well above optimal annealing. The enzyme remains inactive until the time, temperature, and ... after a 15 minute activation step at 95C, the enzyme stays activated in the reaction. Since the enzyme is chemically modified for hot start (no ...Company:GeneChoice, Inc.
Description:... the identical cleavage site as the native enzyme (AspAspAspAspLys↓) and has similar enzymatic activity. Novagen&'s enzyme is produced in E. coli and purified to yield ... definition: one unit is defined as the amount of enzyme that will cleave 50 µg of fusion protein in ...
Company:Novagen
DNase I, ds Qualified from Novagen
Description:... In the presence of Mn2+ and absence of Mg2+, the enzyme makes double strand breaks at random sites in ... the resulting fragments can be controlled by the enzyme concentration and incubation time. This method is ... target genes for functional domain analysis. The enzyme also is included in the DNase Shotgun Cleavage ...Company:Novagen
Taq DNA Polymerase -Klein Taq from AppliChem GmbH
Description:... domain of the DNA polymerase-encoding gene. The enzyme reveals a highly active and even more heat-stable ... Repeated exposure to 98 C does not diminish the enzyme activity. Significant activity remains even after ... One unit of activity is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into ...Company:AppliChem GmbH
Description:... the identical cleavage site as the native enzyme (AspAspAspAspLys?) and has similar enzymatic activity (1). Novagen&'s enzyme is produced in E. coli and purified to yield the ... 26,300. One unit is defined as the amount of enzyme that will cleave 50 g of fusion protein in 16 ...
Company:Novagen
Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Description:... α-Amylase is a hydrolytic enzyme that catalyzes the conversion of starch to a ... the assay can detect the activity of this enzyme down to a final concentration of 1 10-4 U/mL ... where one unit is defined as the amount of enzyme required to liberate 1 mg of maltose from starch ...Company:Molecular Probes (Invitrogen)
Red Taq DNA Polymerase from BullsEye from MIDSCI
Description:... and quick identification of reactions to which enzyme was added and allows confirmation of complete ... polymerase and a 5'3' exonuclease activity. The enzyme lacks a 3'5' exonuclease activity. Red Taq DNA Polymerase leaves an A overhang, which makes the enzyme ideal for TA cloning. ...Company:MIDSCI
AmpliTaq Gold DNA Polymerase from Applied Biosystems
Description:... chemical moiety is attached to the enzyme, the enzyme is inactive. During set-up and the first ramp of ... through non-optimal annealing temperatures), the enzyme is inactive. The result is that mis-primed ... chemical hot-start capability can release active enzyme in a "time-release" manner. With or without a ...Company:Applied Biosystems
TURBO DNase (2 U/l) from Ambion
Description:... of the protein for DNA. The result is a versatile enzyme that has a 6-fold lower Km for DNA, and an ... wild-type enzyme. As a result, TURBO DNase is the enzyme of choice for eliminating plasmid DNA templates ... very low concentrations of DNA means that the enzyme is particularly effective in removing trace ...Company:Ambion
TACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem
Description:... Kit ADAM17 Activity Kit TNF-α Converting enzyme Activity Kit 96 Tests. PROTECT FROM LIGHT. ... lysates and biological samples and for screening enzyme inhibitors. An Anti-Human TACE-Coated 96-Well ... level of fluorescence is directly related to the enzyme activity. reference: Kirkegaard, T., et al. ...Company:Calbiochem
Description:... AgarACE Enzyme(a) is a unique agarose-digesting enzyme developed by Promega for simple and quantitative ... of intact DNA or RNA from agarose gels. The enzyme is sufficiently thermostable that low melting ... slight modifications to the protocol, AgarACE enzyme can also effectively hydrolyze non-LMP agaroses, ...
Company:Promega
GPDH Assay Kit from Takara Mirus Bio
Description:... The enzyme glycerol 3-phosphate dehydrogenase catalyzes a ... into adipocytes and, thus, the activity of this enzyme has been used as an index for monitoring fat ... involves extracting cells by addition of the enzyme Extraction Buffer, followed by serial dilution of ...Company:Takara Mirus Bio
NovaTaq DNA Polymerase from Novagen
Description:... recombinant form of Thermus aquaticus DNA polymerase. This thermostable enzyme is suitable for a wide range of PCR applications. To ensure the highest ... exonuclease and lacks 3′→5′ exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning ...Company:Novagen
NovaTaq DNA Polymerase from Novagen
Description:... recombinant form of Thermus aquaticus DNA polymerase. This thermostable enzyme is suitable for a wide range of PCR applications. To ensure the highest ... exonuclease and lacks 3′→5′ exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning ...Company:Novagen
SuperFreeze Peroxidase Conjugate Stabilizer from Pierce Biotechnology, Inc.
Description:... Features Provides a buffered anti-freeze environment for enzyme conjugated antibodies and proteins Maintains a liquid, low viscosity ... to prevent anti-microbial growth during long-term storage Protect enzyme conjugates during storage without the need to ....Company:Pierce Biotechnology, Inc.
Apo-BrdU Apoptosis Detection Kit from eBioscience
Description:... individual steps in the assay; terminal deoxynucleotidyl transferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), and fluorescein labeled ... ends with bromolated deoxyuridine triphosphate nucleotides (Br-dUTP). The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes a template ...Company:eBioscience
Apo-Direct Apoptosis Detection Kit from eBioscience
Description:... individual steps in the assay; terminal deoxynucleotidyl transferase enzyme (TdT), Fluorescein-deoxyuridine triphosphate and propidium iodide/RNase A ... fluorescein- deoxyuridine triphosphate nucleotides (FITC-dUTP). The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes a ...Company:eBioscience
GeneAmp AccuRT RNA PCR Kit with Controls from Applied Biosystems
Description:... A thermostable, thermoactive designer enzyme that enables RT-PCR in a one-step, single-tube-reaction. AccuRT RNA PCR enzyme allows reverse transcription and PCR amplification to be performed at ...Company:Applied Biosystems
Description:... are resistant to degradation except with extremely high concentrations of enzyme (1). The enzyme is used to remove single-stranded termini from double-stranded DNA (2), ...
Company:Promega
Dithio-bis(2-nitrobenzoic acid) BioChemica from AppliChem GmbH
Description:... a synthetic substrate, replacing the natural substrate acetylcholine. This enzyme hydrolyses the substrate to yield acetate and thiocholine. The free thiol ... each time. Literature: *(1) John, R.A. (1993) Photometric assays in enzyme Assays: A Practical Approach p. 81 (Eisenthal, R. & Danson, M.F. eds.) ...Company:AppliChem GmbH
Amphetamine ELISA Kit, Ultra from Neogen Corporation
Description:... controls to the antibody-coated microplate followed by the addition of the enzyme conjugate (HRP). During an incubation, the drug in the sample and the drug enzyme conjugate compete for binding sites on the antibody-coated microplate. ...Company:Neogen Corporation
Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Description:... a-Amylase is an enzyme that catalyzes the hydrolysis of starch to a mixture of maltose, ... of 2 10-3 U/mL, where one unit is defined as the amount of enzyme required to liberate 1 mg of maltose from starch in 3 minutes at 20C, pH ....Company:Molecular Probes (Invitrogen)
Human TRAP 5 ImmunoCapture Assay Kit from BioVendor Laboratory Medicine, Inc.
Description:... An immuncapture enzyme-activity assay based on measurenment of enzyme activity of TRAP 5 captured on monoclonal antibody coated microtiter ... includes two incubation steps (1st standards, samples or controls, 2nd enzyme reaction) separated by a washing step. The assay is intended for the ...Company:BioVendor Laboratory Medicine, Inc.
Human Topoisomerase II, Alpha from USB Corp.
Description:... (1,2) . As a result of its double-stranded DNA passage mechanism, the enzyme can relax negatively or positively supercoiled DNA, as well as ... , 2.5% glycerol, 0.5mM ATP. Unit Definition: One unit is the amount of enzyme required to fully relax 0.3 g (5nM) of negatively supercoiled pBR322 ...Company:USB Corp.
AMV Reverse Transcriptase from Promega
Description:... primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in ....Company:Promega
DNA Polymerase I, E. coli from EPICENTRE Biotechnologies
Description:... DNA Polymerase I from E. coli is a DNA-dependent DNA polymerase. The enzyme also contains both 5'--3'and 3'--5' exonuclease activities.1 The 5'?3' exonuclease activity enables the enzyme to use nicks and gaps in the DNA as starting points for labeling the DNA ...Company:EPICENTRE Biotechnologies
Human Topoisomerase II, Alpha from USB Corp.
Description:... (1,2) . As a result of its double-stranded DNA passage mechanism, the enzyme can relax negatively or positively supercoiled DNA, as well as ... , 2.5% glycerol, 0.5mM ATP. Unit Definition: One unit is the amount of enzyme required to fully relax 0.3 g (5nM) of negatively supercoiled pBR322 ...Company:USB Corp.
AmpliTaq DNA Polymerase, LD with Buffer I from Applied Biosystems
Description:... and Buffer I AmpliTaq DNA Polymerase, LD (Low DNA), is the same enzyme as AmpliTaq DNA Polymerase but is further purified through a proprietary ... will be effecitvely minimized when amplifying bacterial sequences. The enzyme is quality control tested to verify that less than 10 copies of bactieral ...Company:Applied Biosystems
AmpliTaq DNA Polymerase, 1,000 units with Buffer I from Applied Biosystems
Description:... AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and ... DNA polymerase gene in E. coli.1 It is the most thoroughly characterized enzyme available for the polymerase chain reaction (PCR) process and its ...Company:Applied Biosystems
GCpro Taq DNA Polymerase Complete System from GenScript Corporation
Description:... containing the DNA polymerase I gene from Thermococcus litoralis. The enzyme includes a highly processive 5'-3' DNA polymerase activity. Its inherent ... increased fidelity of DNA synthesis compared to Taq DNA Polymerase. The enzyme includes an optimized reaction buffer, which enables the amplification of ...Company:GenScript Corporation
Enterokinase EKMax from Invitrogen
Description:... and isolated from the yeast Pichia pastoris to yield a highly purified enzyme preparation (2). It recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this peptide ...Company:Invitrogen
Benzonase Nuclease HC, Purity > 90% from Novagen
Description:... Benzonase Nuclease is a genetically engineered endonuclease. This unique enzyme degrades all forms of DNA and RNA while having no proteolytic activity. It ... The ability of Benzonase to rapidly hydrolyze nucleic acids makes the enzyme an excellent choice for viscosity reduction to reduce processing time and ...Company:Novagen
PicTure - Double Staining Kit from Invitrogen
Description:... on tissue specimens.PicTure™-Double Staining contains two enzyme polymer conjugates: Gt anti-Mouse IgG-HRP & Gt anti-Rabbit IgG-AP polymer conjugates. Both enzyme polymer conjugates can be applied on the specimen at the same time. This ...Company:Invitrogen
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