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Cleavage in Biological Products

Enterokinase from Novagen

Description:... a serine protease which recognizes the identical cleavage site as the native enzyme (AspAspAspAspLys↓) ... activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a ... rEK Dilution/Storage Buffer • 1 ml 10X rEK cleavage Buffer • 10 ...
Company:Novagen

HRV 3C Protease from Novagen

Description:... 6XHis-fusion protein, which recognizes the same cleavage site as the native enzyme: ... pET-50b(+) incorporate the HRV 3C protease cleavage site in combinations with His•Tag, ... kit_contents: HRV 3C Protease HRV 3C cleavage Control Protein 10X HRV ...
Company:Novagen

PARP Cleavage Detection Kit from Calbiochem

Description:... to detect poly(ADP-ribose) polymerase (PARP) cleavage by Western blotting. PARP is an enzyme implicated ... During apoptosis, PARP is cleaved by caspase-3. cleavage of PARP from the native 116 kDa to 85 kDa is a ... 116 kDa PARP and the 85 kDa apoptosis-related cleavage fragment from human, bovine, rat, and mouse. Kit ...
Company:Calbiochem

Goat Anti-T7 Polyclonal Antibody, Agarose Conjugated from GeneTex

Description:... [Agarose Immobilized] Goat Immunogen: Goats were immunized with T7 cleavage site (MASMTGGQQMG). Antibody was isolated by affinity chromatography ... cyanogens bromide method. Specificity: Antibody recognizes the T7 cleavage ....
Company:GeneTex

In Situ Pan-Caspase Assay Kit from CHEMICON

Description:... that are triggered in response to pro-apoptotic signals and result in the cleavage of protein substrates, causing the disassembly of the cell. Caspases ... or by employing fluorochrome substrates which become fluorescent upon cleavage by the ....
Company:CHEMICON

In Situ Pan-Caspase Assay Kit, Sulforhodamine from CHEMICON

Description:... that are triggered in response to pro-apoptotic signals and result in the cleavage of protein substrates, causing the disassembly of the cell. Caspases ... or by employing fluorochrome substrates which become fluorescent upon cleavage by the ....
Company:CHEMICON

Thrombin from Novagen

Description:... is supplied as a solution in 50% glycerol and includes 10X Thrombin cleavage Buffer and a cleavage Control Protein. RTECS XO8950000, CAS 9002-04-4, M.W. 37,000. Unit ...
Company:Novagen

DNase I, ds Qualified from Novagen

Description:... of bovine pancreatic DNase I that can be used for double stranded cleavage of DNA molecules. In the presence of Mn2+ and absence of Mg2+, the enzyme ... domain analysis. The enzyme also is included in the DNase Shotgun cleavage Kit, which contains reaction buffer and MnCl2 solution. Storage buffer is ...
Company:Novagen

Enterokinase from Novagen

Description:... of bovine enterokinase, a serine protease which recognizes the identical cleavage site as the native enzyme (AspAspAspAspLys?) and has similar enzymatic ... to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC ...
Company:Novagen

Factor Xa Protease from QIAGEN

Description:... For cleavage of 6xHis tags from proteins containing a Factor Xa Protease recognition site Efficient 6xHis tag cleavage (greater than 90%) ...
Company:QIAGEN

IMPACT-CN System from New England Biolabs

Description:... site which allows the target gene to be cloned immediately adjacent to the cleavage site of the intein tag; this results in the purification of a target ... or N-terminus, respectively. Addition of extra residues may help the cleavage reaction for some target proteins. pTYB2 and pTYB12 contain the same or ...
Company:New England Biolabs

Rabbit Anti-DDDDK Tag Antibody, Agarose Conjugated from MBL International

Description:... Anti-DDDDK-Tag (Enterokinase cleavage Site), Agarose Conjugate Research Focus: tag Storage: 4C Shipping Temperature: ...
Company:MBL International

Mouse Anti-Presenilin 2 Monoclonal Antibody, Unconjugated, Clone APS 26 from GeneTex

Description:... APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Two alternative ...
Company:GeneTex

CCF4-AM from Invitrogen

Description:... and CCF4-AM) of the negatively charged fluorescent beta-lactamase substrates, CCF2 and CCF4. These lipophilic esters readily enter the cell, where cleavage by endogeneous cytoplasmic esterases rapidly converts them into their negatively charged forms, thereby trapping them in the ....
Company:Invitrogen

CCF4-AM from Invitrogen

Description:... and CCF4-AM) of the negatively charged fluorescent beta-lactamase substrates, CCF2 and CCF4. These lipophilic esters readily enter the cell, where cleavage by endogeneous cytoplasmic esterases rapidly converts them into their negatively charged forms, thereby trapping them in the ....
Company:Invitrogen

Mouse Anti-Presenilin 2 Monoclonal Antibody, Unconjugated, Clone 198C679.2.1 from GeneTex

Description:... APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Two alternative ...
Company:GeneTex

Smac/DIABLO, Human, Recombinant, E. coli from Calbiochem

Description:... including XIAP (Cat. No. PF137). Biological activity: IC 50 ~500-1500 nM to reverse the inhibition of XIAP (500 nM) on Z-DEVD-AFC (Cat. No. 264150) cleavage in activated cell extracts. Purity: ≥95% by SDS-PAGE. M.W. 22,000. ...
Company:Calbiochem

CaspSCREEN from CHEMICON

Description:... 110 (D2R), a reported substrate for members of capase family proteases. The rhodamine 110 conjugated substrate is non-fluorescent, however, upon cleavage of the substrate by cellular caspases or apoptosis-related proteases, the released rhodamine 110 gives rise to fluorescence that can be measured at ...
Company:CHEMICON

Mouse Anti-Human Xanthine Oxidase / Aldehyde Oxidase Ab-2 Mouse Monoclonal Antibody, Biotin Conjugated, Clone 145.4 from Lab Vision

Description:... is reversibly converted to the oxygen radical producing XO by oxidation of essential sulfhydryl groups or irreversibly through limited proteolytic cleavage of the amino-terminus. AO has a higher affinity for aldehydes than purines, but produces O in a manner very similar to XO because both oxidases ...
Company:Lab Vision

Human Caspase-7 from Calbiochem

Description:... ICE-LAP3 Mch3 Lyophilized solid. Highly active enzyme useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. Specific activity: 15,000 units/mg protein. One unit is defined as the amount of enzyme that will ...
Company:Calbiochem

AcTEV Protease from Invitrogen

Description:... effective way to remove solubility, secretion, detection, and purification tags from recombinant proteins. AcTEV Protease features Highly specific cleavage activity at the TEV recognition site Enhanced enzyme stability for prolonged protease activity Activity over a broad temperature range (+4C to ...
Company:Invitrogen

S1 Nuclease from Promega

Description:... with extremely high concentrations of enzyme (1). The enzyme is used to remove single-stranded termini from double-stranded DNA (2), for selective cleavage of single-stranded DNA and for mapping RNA transcripts ....
Company:Promega

RNase ONE™ Ribonuclease from Promega

Description:... ONE Ribonuclease may be used to remove RNA from DNA preparations, for RNase protection assays and for mapping or quantitation of RNA by selective cleavage of single-stranded ....
Company:Promega

TACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem

Description:... the plate is washed, and the activity of captured TACE is measured using an internally quenched fluorescent substrate, MCA-KPLGL-Dpa-AR-NH2. cleavage of the scissile amide bond, G-L, releases the fluorophore from the quenching molecule, Dpa, resulting in an increase in fluorescence. Fluorescence of ...
Company:Calbiochem

20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON

Description:... that recognize the substrate LLVY (Meng et al. 1999). The assay is based on detection of the fluorophore 7-Amino-4-methylcoumarin (AMC) after cleavage from the labeled substrate LLVY-AMC. The free AMC fluorescence can be quantified using a 380/460 nm filter set in a fluorometer. A proteasome ...
Company:CHEMICON

pTriEx™-4 DNA from Novagen

Description:... Native protein can be expressed by cloning into the Nco I site, or native protein can be generated by cloning into the Psh AI or Sma I sites and cleavage of the fusion protein with Enterokinase or Thrombin, respectively. The pTriEx-4 vector is also available as an Ek/LIC Cloning Kit . 1 patent ...
Company:Novagen

Mast Cell Degranulation colorimetric Assay Kit from CHEMICON

Description:... and convenient means for assaying tryptase activity. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate tosyl-gly-pro-lys-pNA. The free pNA can then be quantified using a spectrophotometer or a microtiter plate reader at 405 ...
Company:CHEMICON

pET GST Fusion System 41 from Novagen

Description:... other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the generation of native proteins with their authentic ...
Company:Novagen

pET GST Fusion System 41 plus Competent Cells from Novagen

Description:... other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the generation of native proteins with their authentic ...
Company:Novagen

pET GST Fusion System 42 from Novagen

Description:... other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the generation of native proteins with their authentic ...
Company:Novagen

ES Cell Characterization Kit from CHEMICON

Description:... ES and embryonic carcinoma (EC) cells. SSEA-1, a carbohydrate antigen, is a fucosylated derivative of type 2 polylactosamine and appears during late cleavage stages of mouse embryos. It is strongly expressed by undifferentiated, murine ES cells [Solter and Knowles, 1978; Gooi, 1981]. Upon ...
Company:CHEMICON

Rabbit Anti-Aph-1aL, S Loop (92-115) Polyclonal Antibody, Unconjugated from Covance Research Products, Inc

Description:... of presenilin, nicastrin, Aph-1 and Pen-2; all four proteins are necessary for full proteolytic activity. The gamma secretase complex catalyzes cleavage of a number of integral membrane proteins including Amyloid precursor protein, Notch, Erb4 and E-cadherin. While Aph-1 stabilizes the presenilin ...
Company:Covance Research Products, Inc

PARP Cleavage Immunoblotting Assay Kit from Upstate

Description: Kit Components: Anti-PARP LumiGLO Chemiluminescent Substrate Immunoblot Blocking Reagent Pyronin Y Reagent Goat Anti-Rabbit IgG, HRP conjugate Double-It Reagent Film Exposure Folders Autorad Orientation Markers ...
Company:Upstate
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