GeneChoice TEMPase II DNA Polymerase from GeneChoice, Inc.
Description:... useful for performing automated hot start amplification due to its thermal activation at a temperature well above optimal annealing. The enzyme remains inactive ... the reaction reaches optimal annealing temperatures after a 15 minute activation step at 95C, the enzyme stays activated in the reaction. Since the ...AmpliTaq Gold DNA Polymerase from Applied Biosystems
Description:... annealing temperatures, the chemical moiety is cleaved during a heat activation step, releasing active AmpliTaq® DNA enzyme. The yield of specific ... enzyme in a "time-release" manner. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match ...Anti-JIP 1 / 2 (SH3) Polyclonal Antibody, Unconjugated, Clone ZMD.177 from Invitrogen
Description:... is one of three identified MAP kinase pathways in mammals. JNK pathway activation occurs in response to environmental stress, is associated with the nuclear ... of JNK, thereby preventing its nuclear accumulation and the subsequent activation of the JNK pathway. This observation led to the original hypothesis that ...Mouse Anti-Factor V Heavy Chain Monoclonal Antibody, Unconjugated from GeneTex
Description:... Mouse monoclonal to Factor V Heavy Chain Immunogen: Purified human Factor V. Specificity: This antibody reacts with the activation C peptide (mw ~150,000) in the heavy chain of thrombin-cleaved human Factor V. KD = 1.15 x 10 -10 ....Molecular Immunology from Promega
Description:... B.D. Hames and D.M. Glover (1996) • This issue covers T-Cell antigen receptor genes; T-Lymphocyte signal transduction; B-Cell activation antibody engineering and the complement system and other immunological control ....Rabbit Protein Phosphatase 1 (PP1) from Upstate
Description:... MnCl2, 1mM MgCl2, 1mM EGTA, 10 Properites: For selective in vitro dephosphorylation of proteins Purification: DEAE chromatography, divalent cation activation plus trypsin digestion, and gel filtration chromatography Quality Assurance: routinely evaluated by dephosphorylation of proteinsRat Plasmin from Pel-Freez Biologicals
Description:... Prepared from plasminogen by activation with immobilized human ...CaspSCREEN Caspase Screening Kit from BioVision
Description:... Detects caspase activation by flow ....Mouse Anti-JAB1 (Mid) Monoclonal Antibody,, Clone 3F10B10 from Invitrogen
Description:... Clone/PAD: 3F10B10. Immunogen: Synthetic peptide derived from the internal region of JAB1 (Jun activation domain-binding protein 1 CSN5). Specificity: Specific for the JAB1 protein. Reactivity: Human (positive controls: 293T Jurkat cell lysates). ...EGY48/pSH18-34 from Invitrogen
Description:... The Hybrid Hunter Yeast Two-Hybrid System uses the activation of reporter genes to indicate positive protein-protein interactions. Two reporters are used an auxotrophic marker and ....Dynabeads M-280 Tosylactivated from Dynal Biotech
Description:... as proteins and peptides. Hydrophobic, monodisperse magnetic particles (2.8 m in diameter) with p-toluene-sulfonyl (tosyl) groups, further surface activation is not required. Allows easy coupling of antibodies with optimal orientation for affinity purification of proteins. Covalent coupling to primary ...Smac/DIABLO, Human, Recombinant, E. coli from Calbiochem
Description:... ® sequence was expressed in and purified from E. coli . Smac/DIABLO is released along with cytochrome c during apoptosis and promotes caspase activation through the neutralization of IAPs, including XIAP (Cat. No. PF137). Biological activity: IC 50 ~500-1500 nM to reverse the inhibition of XIAP (500 ...A23187, Mixed Calcium-Magnesium Salt from Calbiochem
Description:... Yellow crystalline solid. PROTECT FROM LIGHT. Useful in cell activation experiments when calcium dose-response data are not required. Purity: ≥98% by TLC. Soluble in DMSO or EtOH. For use in aqueous systems, ...SureLINK HRP Conjugation Kit from KPL
Description:... your protein or antibody, including ready-to-use buffers and an optimized storage buffer. Activated HRP is supplied ready-to-use and requires no activation or purification. Sufficient reagents are provided to conjugate 2 samples, each containing between 0.05 - 0.1 mg of antibody or ....Imject Maleimide Conjugation Buffer from Pierce Biotechnology, Inc.
Description:... Maleimide Activated Carrier Proteins are commonly used carriers that have been preactivated with a heterobifunctional cross-linker (Sulfo-SMCC). This activation results in a stable, sulfhydryl-reactive carrier protein. Thus allowing the maleimide-activated carrier protein to conjugate with cysteine residues ...B-Phycoerythrin Labeling Kit-NH2 from Abnova Corporation
Description:... a component of this kit, has succinimidyl group (NHS), and can easily make a covalent bond with amino groups of IgG or other proteins without any activation process. Filtration Tube included in this kit is used for removing small molecules in the sample protein such as Tris buffer and amine compounds that ...Apoptosis Pathway SpliceArrays from ExonHit Therapeutics Inc.
Description:... important role in the regulation of signaling pathways involved in apoptosis and cell death. Splice variants have been reported that affect both the activation and inhibition of defined apoptotic pathways. With Apoptosis and Cell Death SpliceArrays you can perform a classical expression profiling and at the ...EasyCyte Plus System from Guava Technologies
Description:... to obtain even more data while performing more complex biological studies such as white blood cell phenotyping, cell signaling, cytokine production, activation marker analysis, and multiplex compound screening. Monitoring the interplay of up to four different biological mechanisms simultaneously improves ...Rabbit Anti-JNK1, phospho (Thr183 / Tyr185) Polyclonal Antibody, Unconjugated from GeneTex
Description:... The MAPKs family is also characterized by their requirement for dual phosphorylation at a conserved threonine and tyrosine residue for enzymatic activation and both must be phosphorylated for full enzymatic activation. The closely related ERK1 (44 kDa) and ERK2 (42 kDa) kinases are characterized by their ...FAM FLISP™ Lys-CMK Assay Kit from B-Bridge International
Description:... Leu-CMK FLISP Assay with SR-101 Labeled Substrates (red fluorescence; Ex=590 nm, Em=620 nm) • Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine ...SR-101 FLISP FLISP Leu-CMK Assay Kit from B-Bridge International
Description:... Leu-CMK FLISP Assay with SR-101 Labeled Substrates (red fluorescence; Ex=590 nm, Em=620 nm) • Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine ...FAM FLISP™ Leu-DAP Assay Kit from B-Bridge International
Description:... Spacer Leu DAP FLISP Assay with FAM-Labeled Substrate (green fluorescence; Ex=488 nm, Em=530 nm) • Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine ...FAM FLISP™ Spacer-Leu-CMK Assay Kit from B-Bridge International
Description:... Spacer Leu-CMK FLISP Assay with FAM-Labeled Substrate (green fluorescence; Ex=488 nm, Em=530 nm) • Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine ...Human MMP-14 [MT1-MMP], Catalytic Domain, E. coli recombinant from CHEMICON
Description:... CC1041 is a recombinant 177 amino acid polypeptide sequence corresponding to Tyr89 Gly265 of mature human MT1-MMP. Produced by activation of a recombinant soluble proform of MT1-MMP purified from E. coli periplasm. SPECIFIC ACTIVITY:The specific activity of MT1-MMP catalytic domain is ...Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Description:... a number of biochemical reactions, such as DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters.1 The EnzChek Pyrophosphate Assay Kit is a modification of our EnzChek Phosphate Assay Kit (E-6646), ...Factor B-depleted serum from Quidel
Description:... therefore ideal for the detection and quantitation of hemolytically active complement components. Depleted sera have been used to assess complement activation in non-human species. A copy of the monograph "Quantitation of C3 activity in Murine Sera by Hemolytic Assay "is available from Quidel's technical ...Osteoclasts Culture Kit from B-Bridge International
Description:... collagen degraded. Osteoclast differentiation and function is controlled primarily by Macrophage Colony-Stimulating Factor (M-CSF) and Receptor for activation of Nuclear Factor Kappa B Ligand (RANKL). The absence of NF-Kappa B leads to the bone disease osteopetrosis and the absence of M-CSF results in the ...ABsolute QRT-PCR SYBR Green Fluorescein Mix from ABgene
Description:... - Thermo-Start DNA Polymerase remains completely inactive during the reverse transcription stage of QRT-PCR. Enzyme is fully activated by a 15minute activation at 95C. Simple protocol Easily adapted for automation This mix with Fluorescein included is ideal for use in Bio-Rad instruments - other formats ...microRNA Expression Profiling - Comprehensive Service from LC Sciences
Description:... the Germline. Science 313(5785), 320-324. (2) Saito, Y., Liang, G., Egger, G., Friedman, JM., Chuang, JC., Coetzee, GA., Jones, PA. (2006) Specific activation of microRNA-127 with downregulation of the protooncogene BCL6 by chromatin-modifying drugs in human cancer cells. Cancer Cell 9(6), 435-443. (3) ...Mast Cell Degranulation colorimetric Assay Kit from CHEMICON
Description:... skin tissue (He, 2004; He, 1998; Schwartz, 1986). Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases. The Chemicon Mast Cell Degranulation Assay Kit provides a quick, efficient and ...Monocyte Activation Kit from 3H Biomedical AB
Description: Complete cell culture media with ...