Oligonucleotide in Biological News

A new gene silencing platform -- silence is golden

... polyA tail addition in a gene specific manner. Further, the domains of the oligonucleotide are independent so transcript binding and U1 snRNP binding can be ... used 5'-end-mutated U1 snRNA," Gunderson explained. "The U1 Adaptor is an oligonucleotide version of this older method and instead targets the 3' end processing ...

Leroy Hood to receive 2008 Pittcon Heritage Award

... genomics: the DNA and protein sequencers and synthesizers and the ink-jet oligonucleotide synthesizer. The DNA sequencer in particular has revolutionized genomics ... at the University of Washington, where he developed the ink-jet oligonucleotide synthesizer, which synthesizes DNA chips, and initiated systems studies on ...

ISU researchers find possible treatment for spinal muscular atrophy

... because of the presence of a specific intronic sequence in the gene or DNA. To make SMN2 behave as SMN1, Singh has introduced a small antisense oligonucleotide that blocks this specific intronic sequence. When the intronic sequence is blocked, SMN2 produces normal proteins and acts, in effect, like SMN1. ...

Strong immune response to new siRNA drugs in development may cause toxic side effects

... Inc. (www.liebertpub.com) The article is available free online at www.liebertpub.com/oli siRNAs are duplex structures comprised of short oligonucleotide sequences. The discovery that naturally occurring and synthetic siRNAs can effectively prevent expression of a disease gene sparked intense interest ...

Researchers take first look at the genetic dynamics of inbreeding depression

... researchers also crossed the three highest inbred lines to one another, creating outbred lines that could be compared with the inbred ones. Using oligonucleotide microarrays, which can measure the activity of all of an organism's genes at once, the researchers were able to see which genes were more or less ...

Less can be more, for plant breeders too

... crops and it may provide an important new avenue to enhance agronomic performance and nutritional value. Computer software to design the required oligonucleotide sequences and detailed protocols to produce amiRNAs are provided free of charge on the authors web site, at http://wmd2.weigelworld.org. Similarly, ...

Different microarray systems more alike than previously thought

... another commercial platform called two-color long oligonucleotide arrays. In general, the study showed Affymetrix ... came from one of the labs using a two-color long oligonucleotide array, with better statistical results in ... However, the other lab using the two-color long oligonucleotide array had the worst overall performance. But with ...

Kinovate Life Sciences Launches Nittophase?High Performance Solid Support For Oligonucleatide Synthesis

... Life Sciences, Inc, a provider of tools for oligonucleotide synthesis and gene delivery, announced today that ... launch its new NittoPhase?solid support for oligonucleotide synthesis at the TIDES meeting commencing in ... of Nitto Denko Corporation, Isis?outstanding oligonucleotide synthesis and drug development expertise and the ...

Cellular Defects in Premature Aging Disease are Reversible

... said Scaffidi. The NCI researchers designed a chemically stable DNA oligonucleotide ?a short DNA sequence that the cell wouldn't be able to degrade ?that ... been eliminated and replaced with the properly spliced counterpart. The oligonucleotide was highly specific to the mutated region and did not cover-up other ...

Study: 'homemade' gene expression technology unreliable

... The three-year, NIEHS-funded study compared a lab-built spotted long oligonucleotide microarray, and a commercially produced long oligonucleotide microarray. The two types were represented among 12 microarray platforms ...

DOE JGI sets 'gold standard' for metagenomic data analysis

... best hit and assume that the sequence queried belongs to the same group of organisms. Other methods use intrinsic features of the sequence, such as oligonucleotide frequencies. Patterns of these features help to discriminate between the possible groups of organisms that contributed the sequence. Several ...

Embryonic stem cells accrue genetic changes

... M.B.B.S., Ph.D., of the Laboratory of Neurosciences at the National Institute on Aging. The scientists used so-called GeneChip microarrays, or oligonucleotide arrays, to determine whether there were genetic differences between the early and the late batch of each of the stem cell lines, including whether ...

NHGRI expands effort to revolutionize sequencing technologies

... (2 years) "Bead-Based Polony Sequencing" Supplemental funding is expected to accelerate commercialization of this technology that will use oligonucleotide ligation to read DNA sequence, using bead-based, polymerase colony (polony) sequencing technology. Vera B. Gorfinkel, Ph.D., The State University ...

High-powered gene profiles provide clues to genes involved in common form of lung cancer

... containing about 11 genes, where gene deletions or over-copying had occurred. Researchers re-analyzed the tumor and cell samples with the latest oligonucleotide expression microarray technology from Affymetrix, which indicates if individual genes are active. Using this data, they scanned the genes in these 93 ...

Health costs soar as 60 million Americans classed as obese

... the researchers have also shown that the effects of the DNA constraint on the RNA structure can be modulated by external stimuli such as DNA oligonucleotide strands, protein enzymes and chemical reagents. While Silverman and Miduturu are currently using RNA as a proof of principle for their DNA ...

UN successfully tests 'green' pesticide against locusts

... the researchers have also shown that the effects of the DNA constraint on the RNA structure can be modulated by external stimuli such as DNA oligonucleotide strands, protein enzymes and chemical reagents. While Silverman and Miduturu are currently using RNA as a proof of principle for their DNA ...

DNA constraints control structure of attached macromolecules

... the researchers have also shown that the effects of the DNA constraint on the RNA structure can be modulated by external stimuli such as DNA oligonucleotide strands, protein enzymes and chemical reagents. While Silverman and Miduturu are currently using RNA as a proof of principle for their DNA ...

Novel technology detects human DNA mutations

... do so, Nanosphere scientists employ a two-step process called ClearReadTM technology. This method sandwiches a target DNA SNP segment between two oligonucleotide sequences to greatly increase detection specificity and sensitivity. One segment identifies any mutations in the DNA and the probe, a highly ...