medium

Bioartificial kidney under study at MCG

...tions also can harm the kidneys. Even the contrast medium needed to study kidney function can be toxic. "I have seen healthy people on antibiotics for urinary tract infections develop renal failure; that's not uncommon, but it's most dreaded in the critically ill patient," Dr. Szerlip says. ...

Wisconsin scientists grow critical nerve cells

...es of mini-stages, each requiring a unique growing medium and precise timing. To start, the Wisconsin team generated neural stem cells from the embryonic stem cells. They then transformed the neural cells into progenitor cells of motor neurons, which in turn developed in a lab dish into spinal motor neuron ...

Current human embryonic stem cell lines contaminated UCSD/Salk team finds

... cells," said Gage."Also, components of the growth medium have the capacity to change theimmunological characteristics of the human ES cells. More research isneeded to understand the optimal conditions for preparing human cellsfor therapeutic application.""We considered that one partial solution to the prob...

UCSD researchers maintain stem cells without contaminated animal feeder layers

...be rather simple now to develop a specific defined medium that allows for the maintenance of the human stem cells and enhances research without the problem of contaminating animal cells and their products." In a summary to their paper, the researchers noted that "the identification of activin A as a key fa...

The BIOS Initiative - open source biotechnology is born

... establish, will allow the public-sector, small to medium enterprises and even large firms worldwide to explore new business models and begin a new era of transparent and cost-effective innovation in life sciences. The technologies include TransBacter, a new method for transferring genes to plants, and GUS...

UF Researchers Map Bacterial Proteins That Cause Tooth Loss

...proteins secreted by P. gingivalis when grown in a medium containing human gum cell proteins with the proteins produced by the bacteria when grown in a neutral medium. Bacterial proteins from the two conditions were separated using a new technique called Multidimensional Protein Identification Technology, o...

PCRM develops world's first cruelty-free insulin assay

...ement for the fetal calf serum used as a culturing medium in millions of medical tests every year. After mo... successfully incorporated antibodies grown in the medium into a test kit for human insulin. A report on the new methodology will be published soon in a peer...

Researchers devise way to mass-produce embryonic stem cells

... another chamber that holds fluid, or medium. This medium delivers chemical messengers, called cytokines, to the stem cells. The cytokines essentially tell the stem cells to stay in their undifferentiated state. The difference between growing stem cells in a flask vs. the bioreactor is that the cells grown...

Nature provides inspiration for important new adhesive

...be used in making softwood plywood, particleboard, medium density fiberboard, oriented strand board, and the laminated veneer lumber that is finding increasing use to replace conventional joists and beams in construction. Techniques have also been explored to create the new adhesives from tree bark or wood...

Human Eggs Can Develop From Ovarian Surface Cells In Vitro

...o 6 days in the presence of an estrogen-containing medium (phenol red) to stimulate their growth, the team was able to produce new human oocytes in vitro. The oocytes cultured in this way are viable and went on to successfully complete the first meiotic division to become mature human eggs -- capable of be...

Scientists succeed in cloning human embryos from eggs matured in the lab

...tes were artificially activated by incubation in a medium containing calcium ionophore, which enabled the injected nucleus to prepare for the first embryonic division. Mr Heindryckx said: "Eighteen out of the 25 in vitro matured MII oocytes survived this nuclear transfer. Of these, 11 showed the formation ...

Invitrogen Launches i-Path -- A Unique Systems Biology Platform at BIO 2005

...tes were artificially activated by incubation in a medium containing calcium ionophore, which enabled the injected nucleus to prepare for the first embryonic division. Mr Heindryckx said: "Eighteen out of the 25 in vitro matured MII oocytes survived this nuclear transfer. Of these, 11 showed the formation ...

Aptamer-based Biosensor screens Air Force personnel and equipment

...ance, "no threat" is green, a barely detectable to medium level of an agent is one or two yellow dots, and a high detection level is red. The biosensor system is designed to be reliable, disposable and cost-effective. The Air Force will continue testing the device over the next several months. ...

Visceral Leishmaniasis: Successful Vaccine Trial In Dogs

...re J.L. (1999). Leishmania Spp: completely defined medium without serum and macromolecules (CDM/LP) for the continuous in vitro cultivation of infective promastigote forms. Am. J. Trop. Med. Hyg., 60 (1), 41-50. Brevets : Lemesre J.L. (1993). "Procédé de culture in vitro de différents stades parasitaires ob...

Student scientists create living bacterial photographs

...ng in dishes of agar, a standard jello-like growth medium for bacteria. Like pixels on a computer screen switching between white and black, each bacterium either produced black pigment or didn't, based on whether it was growing in a light or dark place in the dish. The resulting images are a collection of...

Life in deadly conditions

...produce vitamins and amino acids. Thus, the growth medium for the culture of the single-celled organism could be significantly simplified. Dieter Oesterhelt explains that "the comparison with other halophile archaea we have studied shows that these organisms have a high plasticity with which they can adapt...

War on terror meets war on cancer

...re were four sets of flasks, each holding a growth medium containing water with a different ratio of oxygen-18 to oxygen-16. After the bacteria grew for three hours, the contents of each flask were sucked through a filter, leaving a pasty "cake" of bacterial cells with the consistency of wet flour. Water ...

Sperm stem cells closer to being like embryonic stem cells

...hroughs in this study was developing a new type of medium to grow the cells in, and another was the use of a genetically manipulated "tag" that specifically labeled germ cells with a green fluorescent protein, making the germ cells easier to identify when mixed with other cell types. "The rat testes conta...

Looking for the genes that affect a person's level of response to alcohol

...ny different genes, each contributing a small to a medium amount," said Hesselbrock. "What Dr. Schuckit has done is add another set of genes to that pool. It may be a small step, but that certainly doesn't diminish the importance of the finding." ...

Genes involved in biofilms

... second strategy arose during the development of a medium chemically defined in order to study how environmental signals affect the process of formation of the biofilm. Unexpectedly, most of the clinical strains of Staphylococcus aureus were unable to form biofilm in the synthetic medium, which suggested ...

Predators keep the world green, ecologists find

...ng contrast with this scenario of destruction, the medium islands presented a relatively normal appearance." Besides proving that the green world hypothesis is correct, Terborgh's team's results have important implications for the debate raging in many countries over reintroduction of top predators such as...

Stem cells from muscles can repair cartilage

...t develop into cartilage) depending on the type of medium in which they grew. The addition of transforming grown factor â1 (TGFâ1) to MDSC-B4 did not enhance chondrogenic differentiation. The authors conclude that skeletal muscle is a promising source of cells that can differentiate into cartilage-producin...

New journal article urges use of animal serum-free media for growing live cells

...reakthroughs permit the growth of human cells in a medium free of animal serum, enabling scientists and researchers to make cell culture safer and more humane. "Scientists have access to humane and scientifically superior alternatives, so now is the time to completely eliminate the use of fetal calf serum...

Study finds drug may cut down involuntary movements

...tive disorder that worsens as brain cells known as medium spiny neurons are killed off by a mutant protein. The disease brings with it an array of other difficulties as well, including cognitive problems, changes in personality, and psychiatric problems like depression. As many as one-quarter of patients ...

Behavioral studies show UV contributes to marsupial color vision

...lly distinct cone types, sensitive to short (SWS), medium (MWS), and long (LWS) wavelengths, is a common feature of Australian marsupials. However, since evidence of color vision cannot be derived from physiological studies alone, marsupial trichromacy remained to be established with an unequivocal behaviou...

Findings advance use of adult stem cells for replacement bone

... of having the stem cells create the proper growth medium themselves. The group, which included graduate student Quynh Pham and postdoctoral research associate Upma Sharma, accomplished this by seeding discs of titanium mesh with stem cells and encouraging them to form extracellular matrix, or ECM, the bone...

Strawberries by design

...original site and transferring it to an artificial medium for growth or maintenance. Here unfolded leaves, known as trifoliate leaves, are able to grow. When collected at 6-7 weeks after seed germination, these leaves are highly amenable to gene transfer using A. tumefaciens. Since they glow green under flu...

Wisconsin scientists grow two new stem cell lines in animal cell-free culture

...clinical reality by completely ridding the culture medium in which they are grown of animal products that co...new cell lines in completely defined conditions in medium that completely lacks animal products." Other groups, Thomson notes, have cultured established huma...

First direct mechanical communication of mitochondria, cardiomyocyte nucleus shown

...iment, the researchers found that in an artificial medium mimicking the cytosol, 10 micro-molar of valinomycin (a potassium ionophore that induces mitochondrial matrix swelling) decreased nuclear volume by a significant 12% ± 2%. And 150 micro-molar of diazoxide (a mitochondrial ATP-sensitive potassium chan...

How IVF could be causing genetic errors in embryos

... can be modified by the composition of the culture medium and oxygen concentration. "Interestingly, the expression of one gene, H19, was reduced regardless of the culture conditions, and as H19 is associated with Beckwith Wiedeman syndrome, this finding needs to be investigated further." However, he cauti...

Corn waste potentially more than ethanol

...inoculated with domestic wastewater and a nutrient medium containing glucose, the researchers report in the journal Energy and Fuels. Once established, the bacteria colonies were fed the sugary organic liquid obtained from steam exploding of corn stover. The researchers, who include Logan, Yi Zuo, Penn Sta...

Nanotechnology being used to improve biocompatibility of human prosthetics and implants

...ssolution of the Ca/P-rich phases into the culture medium might promote the proliferation/differentiation of the osteoblast cells. ...

Medium is the message for stem cells in search of identities

...shes, specific types of cells would arise. "The medium and the molecular environment influence the fate of the cell," said Dennis Steindler, Ph.D., executive director of the McKnight Brain Institute. "We simulated some events that occur while the brain is developing and challenged them with different env...

Birds going extinct faster due to human activities

...shes, specific types of cells would arise. "The medium and the molecular environment influence the fate of the cell," said Dennis Steindler, Ph.D., executive director of the McKnight Brain Institute. "We simulated some events that occur while the brain is developing and challenged them with different env...

Test reveals effectiveness of potential Huntington's disease drugs

.... It primarily attacks nerve cells called striatal medium spiny neurons, the main component of the striatum. Dr. Bezprozvanny's group previously demonstrated that Huntington's striatal neurons are oversensitive to glutamate, a compound that nerve cells use to communicate with each other. In the lates...

Chemical tests of cell growth enter third dimension

...ining many tiny wells. Each well contains a growth medium and some cells, and a protein that will cause growing cells to fluoresce. Researchers test a drug by adding it to a well. If the cells continue to fluoresce, that means that the cells are still reproducing, and the drug isn't effective at controlling...

Faster, more accurate tuberculosis test developed

... Mycobacterium tuberculosis grows faster in liquid medium than in solid medium; (2) TB grows in characteristic tangles or coils and these characteristic cord formations can be seen microscopically in liquid medium; and (3) the ability to incorporate drugs permits rapid and direct drug-susceptibility testing...

Cholera pathogen reveals how bacteria generate energy to live

...nzyme has to reach all the way from the water-like medium inside the cell, through the oily membrane interior, to the water-like environment outside the cell. For this reason, the enzyme is made up of water-soluble and oil-soluble components within a single entity, so it can’t hold its shape in any one sol...

Groundbreaking Canadian asthma study

...th, a routine procedure. physicians treat small to medium sized airways with Bronchial Thermoplasty (BT). The BT device generates radio frequency/thermal energy and reduces areas of underlying smooth muscle in the airways. The procedure is completed in three treatment sessions, each lasting less than one ho...

Fungal factories may save hemlock forests

...nd other funders. Whey is a far cheaper growing medium than those available in labs for the many fungi now in use as biological controls in agriculture and forestry. And the whey serves as a nutritional resource, making each droplet a cozy biological factory for a fungal colony, pumping spores out int...

Immunization rates hit record high in poor countries

...nd other funders. Whey is a far cheaper growing medium than those available in labs for the many fungi now in use as biological controls in agriculture and forestry. And the whey serves as a nutritional resource, making each droplet a cozy biological factory for a fungal colony, pumping spores out int...

Manual dishwashing study digs up dirt on dish cleanliness

...food. Pascall explained that milk is a good growth medium in the laboratory, but why it adheres to glass so well isn't clearly understood. "Milk is an area of research we'd like to explore further," Pascall said. "We want to find ways to safely and quickly remove milk dried on glasses." The research a...

Making mice with enhanced color vision

...e three visual receptors that absorb short (blue), medium (green) and long (red) wavelengths of light. Mice,... mammals, only have two; one for short and one for medium wavelengths. In the study, the researchers designed a "knock-in" mouse that has one copy of its...

Stem cells act through multiple mechanisms to benefit mice with neurodegenerative disease

...out mouse feeder layers and in a "defined" culture medium that is compatible with clinical use and demonstrating for the first time that such preparations are consistent with a therapeutic impact. Sandhoff results from a genetic mutation that reduces the body's supply of an enzyme, called hexosaminida...

OHSU surgeon implants donated tissue allografts

.... Tissues can be maintained for up to 28 days in a medium that keeps the cells viable and the tissue healthy so it continues to live after transplantation. Cooperation between Community Tissue Services, which has been serving Portland and the greater Oregon community for decades, and the tissue preparat...

Asexual worm quickly adapts to soil contamination

...o the contamination. In the contaminated culturing medium they laid more eggs and lived for longer than the nematodes from the clean soil. Asexual The rapid adaptation to the environment in an asexual species is an interesting finding. It contradicts the general opinion that asexual species have low...

Human stem cell treatment restores motor function in paralyzed rats

...ter paralysis. The other seven were injected with medium that contained no stem cells. The recovery of motor function was evaluated in seven-day intervals, showing a progressive recovery of ambulatory functions in the rats that received stem cells. Three of the nine rats injected with hSSCs returned to ...

Enzyme delivered in smaller package protects cells from radiation damage

...ter irradiation, the cells were plated in a growth medium and incubated at body temperature for 7 days at which time colonies of greater than 50 cells were counted. The MnSOD transfected cells were significantly more resistant to ionizing radiation than the non-tranfected cells. However, there was no si...