injection

Emory Study Tests Bone Marrow Stem Cells to Improve Circulation in Legs

...es.Patients in the clinical trial will be given an injection of either agrowth factor called GM-CSF (granulocyte-macrophage colony stimulatingfactor) or placebo (sterile salt water) three times a week for twoweeks. The level of EPCs in the volunteers' blood will be measuredbefore, during and after administrati...

Controversial drug shown to act on brain protein to cut alcohol use

...ived Ibogaine. The drug was administered either by injection ordirectly into the same brain region where GDNF levels were shown toincrease.The research also showed that Ibogaine was quite effective inpreventing relapse, or "falling off the wagon" -- the vulnerability ofrecovered alcoholics or addicts to return...

Muscle-targeted gene therapy reverses rare muscular dystrophy in mice

...ell to enzyme infusions, treatment requires weekly injection of a large amount of the enzyme. "Gene therapy ha...y in Pompe disease mice. Six weeks after muscular injection with the virus containing the muscle-restricted gene, mice exhibited high levels of the enzyme and r...

Rush Physicians Using Gene Therapy For Heart Patients With Moderate To Severe Chest Pains Who Do Not Benefit From Other Treatments

...e middle muscular layer of the heart wall near the injection site. Inside the cell, the DNA encoded VEGF-2 expresses itself which in turn stimulates the growth of new blood vessels by promoting the proliferation of endothelial cells in the heart. New blood vessels are required to provide oxygen-carrying blood...

HIV vaccine trial breaks ground for future research

...ransmission. The vaccine and placebo were given by injection seven times over 30 months and the participants were assessed for risk. At each visit the participants were tested for HIV infection, and for those who were positive, HIV-1 plasma RNA load and CD4 cell counts were monitored on a regular basis for 24 ...

Gene Therapy Cures Inherited Liver Disease In Rats

... has been completely cured long term with a single injection in an adult animal," said Dr. Brendan Lee, associate professor of molecular and human genetics and a Howard Hughes Medical Investigator at Baylor College of Medicine. Crigler-Najjar syndrome is currently treated by placing the person under special U...

First frozen egg baby born in Canada

...tro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), and the resulting embryos implanted in her uterus. "The patient went through an uncomplicated full-term pregnancy and straightforward delivery," says Dr. William Buckett, the MUHC physician who looked after the mother during the pregnancy. T...

Youth With HIV Take More Risks After New Meds Introduced

... more likely to have had a sexual partner who used injection drugs. The post-HAART group also knew their HIV diagnosis at a younger age and were in worse health than the pre-HAART group, the researchers found. "Given the availability of HAART, it is surprising that the post-HAART youth experienced more sympt...

Used in a new way, RNA interference permanently silences key breast cancer gene

...unable to form breast tumors either at the site of injection or at distant sites typically involved in metastatic breast cancer in this mouse model. Arlinghaus points out that a human therapy based on these findings is not on the horizon because lentivirus delivery systems haven't been approved for human use ...

Turning viruses into allies against cancer

... stages of their disease, received either a single injection of the virus, the volume depending on tumor size, ...venous treatment with this combination followed by injection of radioactive iodine into the mice bearing the human hepatocellular cancer cells resulted in high u...

Basis for DNA ejection from single phage particles

...to the driving force behind this poorly understood injection process, which has been proposed in the past to occur through the release of pressure accumulated within the viral particle itself. Almost all phages (also known as bacteriophages) are formed of a capsid structure, or head, in which the viral genome...

Gene therapy cures inherited liver disease in rats

... has been completely cured long term with a single injection in an adult animal," said Dr. Brendan Lee, associate professor of molecular and human genetics and a Howard Hughes Medical Investigator at Baylor College of Medicine. Crigler-Najjar syndrome is currently treated by placing the person under special U...

UCLA cancer researchers shake loose hidden biomarker

... of the cell membrane, which blood can't reach, so injection of immunotherapy into the bloodstream is not effective," said Rajasekaran, also an associate professor of pathology and laboratory medicine. "By using information from very basic studies about how the PSMA protein is targeted in these cells, we ident...

Discoveries by UAB and Florida scientists may help transplanted organs survive longer

... in rat models, also showed that a single muscular injection of the molecule, interleukin-10 (IL-10), carried in a gene delivery vector, or mechanism, could provide long-term therapeutic effects. Anupam Agarwal, M.D., director of the UAB Nephrology Research and Training Center and senior author of the report,...

High rates of sexually transmitted infections found in young drug users

..., but did not find significant differences between injection drug users and non-injection drug users. Few of th... rate of sexually transmitted infections among the injection and non-injection drug users we recruited, which indicates the extent of sexual risk behaviors among...

Thinking the pain away? Study shows the brain's painkillers may cause 'placebo effect'

...oncentrated salt water solution to cause pain. The injection was made while they were having their brains scann... after the scan during which they received the jaw injection alone. Nine of the participants were classified as "high placebo responders" because they had more ...

Secrets to antibody's success against West Nile Virus surprise scientists

...to increase vulnerability to the virus, they found injection of the new antibodies could boost survival rates of mice infected with the virus to greater than 90 percent. Scientists at Macrogenics are working on the preliminary studies required before the West Nile antibody can be tested in humans. Meanwhile...

No small feat: First ever gene therapy success for muscular dystrophy achieved

...ctor was delivered locally, given by intramuscular injection to different muscles of the leg. With both approaches, muscle cells were able to assimilate and copy the genetic instructions for making mini-agrin. Once produced, the mini-agrin protein functionally took the place of the laminin alpha-2 protein by b...

Methamphetamine Abuse, HIV Infection Cause Changes in Brain Structure

...tted diseases, not only by the use of contaminated injection equipment, but also due to increased risky sexual behaviors,?says Dr. Nora D. Volkow, director of the National Institute on Drug Abuse (NIDA), National Institutes of Health, which helped support the research. “These findings show that methamphetamine...

Experimental shingles vaccine proves effective in nationwide study

... study. Half of the participants received a single injection of the zoster vaccine--a live, weakened form of varicella-zoster virus, the virus responsible for chickenpox; the other half received a placebo vaccine. Neither the researchers nor the participants knew who received vaccine and who received placebo u...

Studies clarify risk factors for mother-to-child transmission of hepatitis C virus

...ivery, infant prematurity, and maternal history of injection drug use were not associated with HCV transmission. The overall rate of transmission of the virus from infected mother to child was 6.2 percent in the European study and 3.6 percent in the U.S. study. "Our results strongly suggest that women shoul...

OHSU researchers discover potential mechanism to repair brain damage linked to Multiple Sclerosis

... Back confirmed in another animal model of MS that injection of the HA into damaged myelin prevented myelin from reforming where it had already been destroyed. Conversely, they showed that reducing HA levels or making the HA inactive allows myelin to once again form. "It is our hope that we can interfere with...

Molecular Partners Required For Appropriate Neuronal Gene Repression

... Back confirmed in another animal model of MS that injection of the HA into damaged myelin prevented myelin from reforming where it had already been destroyed. Conversely, they showed that reducing HA levels or making the HA inactive allows myelin to once again form. "It is our hope that we can interfere with...

Updated data on novel HPV vaccine confirms efficacy in large population

... reported in the trial was local discomfort at the injection site. The study is part of an ongoing phase III program involving more than 25,000 people in 33 countries. For the FUTURE II study, a prospective, randomized, double-blind, placebo-controlled study, women aged 16 to 26 years were randomized to rec...

Bone marrow stem cells may heal hearts even years after heart attacks

...nt. This therapy is safe, similar to an 'own blood injection or transfusion,' and has no side effects. It needs only bone marrow puncture and cell aspiration with subsequent stem cell preparation," he said. Although this study did not look at how stem cells may heal heart attack damage, other studies have sho...

Climate change will affect carbon sequestration in oceans, model shows

...ocean will change with climate change. The direct injection of carbon dioxide deep into the ocean has been sug...ness of oceanic carbon sequestration by the direct injection of carbon dioxide at different locations and depths. They found that climate change has a big impac...

Gene therapy reverses genetic mutation responsible for heart failure in muscular dystrophy

...s, Dr. Xiao's team found that direct intramuscular injection of AAV was effective in transferring a gene into muscle cells in a fairly wide area around the injection site. However, for gene therapy treatments to be successful, particularly for muscular dystrophies w...

How 'Dirt' Could Educate The Immune System And Help Treat Asthma

...ocean will change with climate change. The direct injection of carbon dioxide deep into the ocean has been sug...ness of oceanic carbon sequestration by the direct injection of carbon dioxide at different locations and depths. They found that climate change has a big impac...

Grabbing addiction by the tail

...rast, addicted animals who received an intravenous injection of the artificial peptide displayed no sensitized behavior. "The effect was immediate and very noticeable," said Wang. There are several types of glutamate receptors involved in memory and learning, but because the artificial peptide specifically t...

University of Delaware researchers develop cancer 'nanobomb'

...the nanotubes can provide for the highly selective injection of drugs into individual cells. As they undertook various experiments, however, the team made a startling discovery. "When you put the atoms in different shapes and forms, they take on different properties at the nanoscale," Panchapakesan said. "W...

Magnetic probe successfully tracks implanted cells in cancer patients

...ity and that ultrasound failed to accurately guide injection of the cells into lymph nodes in half of the patie...agnetically labeled stem cells with MRI-compatible injection systems to allow MRI guidance of injection in large animals. The current clinical trial builds on B...

Polymer gel can block toxic leakage problem in gene therapy

...o tumors themselves. However, direct intratumoral injection can cause the gene-bearing viruses to escape their...iny tumor blood vessels created by damage from the injection needle. Yuan said such viral escapes are inevitable since the needle's diameter is 30 times larger ...

Blocking the nerve receptor EP1 in mouse models reduces brain damage caused by stroke

...rain) of mice. A group of control mice received an injection of the solvent used to carry the drugs. The investigators then injected each mouse with NMDA, a drug that stimulates the NMDA receptor. Excessive stimulation of these receptors by NMDA, such as during stroke, leads to nerve cell damage. In mice tha...

Synthetic Protein Eases Arthritis Symptoms in Mice

...scientists used a variety of techniques (including injection of TNF-alpha) to induce arthritis symptoms in mice. Researchers also injected some of the animals with lab-made PLAD (P60 PLAD). “We found that P60 PLAD protein powerfully inhibited the symptoms of TNF-alpha-induced arthritis,?says Dr. Lenardo. P60 P...

Study shows combination of immune substances to be safe

...treatment, patients were given IL-12, initially by injection and then intravenously. That was followed by injections of interferon over the next five days. On average, patients received five such cycles over the course of the study. The drug combination not only proved to be safe ?which was the purpose of th...

Asleep in the deep: Model helps assess ocean-injection strategy for combating greenhouse effect

...nimals to death." The challenge in designing CO2 injection strategies is figuring out how to keep droplets of...ofessor of geological sciences. However, at a high injection rate, seawater full of CO2 droplets would have an average density smaller than that of surrounding s...

Mouse study: New muscle-building agent beats all previous ones

...e muscle group analyzed, and mice treated with one injection a week for two, three or four weeks continued to gain muscle mass. But although the new agent seems quite promising, its advantage in potency also requires extra caution. "We don't know what else the new agent is affecting or whether those effects ...

Stem cell mobilization therapy appears to be ineffective in repairing damage caused by heart attack

...oms. Patients were randomly assigned to receive by injection either a daily dose of 10 µg/kg of G-CSF or placebo (an inactive substance) for 5 days. The patients were treated between February 2004 and February 2005. Treatment with G-CSF produced a significant mobilization of bone marrow stem cells. The resear...

DNA gene vaccine protects against harmful protein of Alzheimer's disease

...er's Disease Center at UT Southwestern. The gene injection avoids a serious side-effect that caused the cance...ly seen in animals with these mutations. The gene injection showed superior results compared to a previous human study in which amyloid-beta 42 protein itself w...

HIV/AIDS rates in Tijuana, Mexico increasing at alarming rate

... that high-risk groups ?men who have sex with men, injection drugs users, sex workers and pregnant women ?are o...IV/AIDS in the border region, needle sharing among injection drug users leads to increased HIV infection, and Tijuana is on a major drug-trafficking route. The ...

New male contraceptive clears hurdle

...y returns if the RISUG is flushed out with another injection that dissolves the gel. Elaine Lissner, director of the nonprofit Male Contraception Information Project in San Francisco, says she is not surprised that American men are watching the RISUG trial with keen interest. She emphasizes that the method ha...

Canine cancer vaccine program shows early promise

...ells and immune stimulant, when administered as an injection into the patient's skin, has been shown to stimulate the immune system to specifically fight against the melanoma cells. Dogs that first had surgery for their melanoma and then received vaccine lived cancer-free for approximately twice as long as d...

Master genetic switch found for chronic pain

...uncomfortable prick of a filament (mechanical); an injection of an inflammation-inducing chemical (inflammatory); or nerve damage (neuropathic). They quantified the animals' response by measuring how long the animals lifted or licked their affected paw in response to the treatments. Ma and his colleagues foun...

Researchers reverse juvenile diabetes in animal model

...ritical part of how diabetes occurs. Subcutaneous injection of dendritic cells into the abdominal/pelvic area near the pancreas and lymph nodes, blocks the T cells as they travel to the pancreas to destroy beta cells. "We did this in mice, giving them six injections over the course of several weeks. The inj...

'Bad' enzymes may wear white hats after stroke

...itor at different times after the stroke. When the injection was given immediately following the stroke, rats showed smaller areas of brain damage. Injections given at three days had no effect, but those given at seven days or 14 days led to more extensive brain damage, compared with rats that did not receive ...

Scientists design potent anthrax toxin inhibitor

...tor in rats. When given in relatively small doses, injection of the inhibitor at the same time as anthrax toxin prevented five out of nine rats from becoming ill. Slightly higher doses of the inhibitor prevented eight out of nine rats from being sickened by anthrax toxin. Nine additional rats were injected wit...

Unique equine cataract surgery offered on routine basis

...lens using an ultrasound probe and administered an injection of intravitreal steroids to control postoperative inflammation of the eye. Professor Knottenbelt said: "This new surgery shows how effectively the latest human cataract surgical techniques can translate successfully to bear on an equine problem. ...

Potential heart benefit found in stem cells

...were thicker than in the animals not receiving the injection (5.9 vs. 3.6 millimeters), and the same difference...myopathy were treated with a combined percutaneous injection (both intramyocardial and intracoronary, either the heart muscle or the heart's artery) of bone marr...

Scientists force viruses to evolve as better delivery vehicles for gene therapy

...han the wild AAV, and two evolved strains survived injection into mice with nearly 1,000 times the level of antibodies required to neutralize the wild virus. By sequencing the survivor strains, the researchers discovered that the capsid proteins of the survivors differed from those of the original strain by o...

Stem cell transplants improve recovery in animal models for stroke, cerebral palsy

...h control animals and those that received a single injection of stem cells were evaluated for a period of up to 2 months. Improvements in stem cell treated animals included enhanced performance across the range of tests, which examined strength, balance, agility and fine motor skills, and also included greate...

Taipan venom no snake oil

...e their prey," Dr St Pierre said. "Firstly, venom injection causes massive blood clots instantaneously followed by paralysis as a result of neurotoxins which eventually immobilise and kill the victim." Dr St Pierre said mammals and snakes naturally produced a small amount of Factor X in their livers. "Snake...

New 'self-exploding' microcapsules could take sting out of drug delivery

...e vaccines are administered. Instead of an initial injection followed by a series of boosters, for instance, certain vaccines could be given in a single shot with the "booster" microcapsules timed to rupture at appropriate intervals. ...

Computer scientist sorts out confusable drug names

... as a documented case in which a patient needed an injection of Narcan but received Norcuron and went into cardiac arrest. A few years ago, the FDA turned to Project Performance Corporation (PPC), a U.S. software company, to ensure they don't approve the names of new drugs that may easily be confused with an...

Trial success for diabetic nerve therapy

... University of Manchester team has discovered that injection of a novel therapeutic that works by stimulating a...ts," he said. "Our tests have shown that a single injection of a DNA-binding protein protected nerve function, stimulated nerve growth and prevented tissue dama...

UF scientists test improved gene therapy method for hereditary heart conditions

...w way of delivering corrective genes with a single injection into a vein holds promise for long-lasting treatme...ene expression in the heart but it was with direct injection into the heart muscle and it was inefficient," said UF pediatric cardiologist Barry J. Byrne, M.D., ...

With few factors, adult cells take on character of embryonic stem cells

...to all an animal's tissues and organs. Following injection into blastocysts, iPS cells also contributed to mouse embryonic development. "The finding is an important step in controlling pluripotency, which may eventually allow the creation of pluripotent cells directly from somatic cells of patients," Yaman...

UC Davis researchers move biotechnology closer to replacing electronic pacemakers

...s' hearts had generated bioartificial nodes at the injection sites. Li explained that, through gene expression, normal muscle cells of the heart were converted into pacemaker cells by a process called transdifferentiation. Studies done two weeks after the injections showed the new nodes were able to take over ...

High-dose flu vaccines appear to safely boost immunity in elderly

... to receive one of the three vaccines or a placebo injection and then were monitored daily for one week afterwa...discomfort, redness or swelling at the site of the injection was more common among those who received the 60 ìg dose than among those who received the 15 ìg dose...

UF scientists restore sight to chickens with blinding disease

...quite a long time to build the vector, develop the injection procedure and figure out how to hatch the eggs. By doing the injection early during development, we actually treat the cells before they become photoreceptors." Infants ...

Septum keeps neurons in synch, can reduce epileptic seizures by 90 percent

...thesia, by stimulating the septum directly with an injection of carbachol, or by using the sensory stimulation method of pinching the tail.) The amplitude and frequency of the theta rhythm of the epileptic rats was significantly altered compared to the control group. In effect, the theta rhythm became faster...