hybridization

Whole genome fine map of rice completed

...oject was to reveal the molecular mechanism of the hybridization advantages, thus providing the theoretical basis f...iological traits, thus revealing the source of the hybridization advantage in hybrid rice. ...

Breakthrough Microarray-based Technology for the Study of Cancer

... incancer can be studied using Comparative genomic hybridization (CGH),which undergoes dramatic chromosomal changes...search community."In the past, comparative genomic hybridization was performed throughthe optical imaging of whole chromosomes, a technique with limitedsensitivity, ...

Researchers rein in regulatory RNAs

...ed several striking characteristics. Northern blot hybridization analyses of six randomly chosen SAT pairs revealed that the SAT loci generated multiple transcripts of various sizes, in contrast to a single transcript that is expected under the traditional one gene-one transcript model. Furthermore, the SATs tende...

Agilent Technologies new genome analysis technology set to accelerate Australia fight against mesothelioma

...s new microarray techniques in comparative genomic hybridization (CGH) will allow researchers to rapidly and reliably identify genetic changes in tumorous cells. It is believed that specific genetic changes may accompany the onset and progression of the disease. Dr. Andrew Holloway of the Peter MacCallum Cancer C...

Motor transport in bio-nano systems

... these transport systems would act to increase the hybridization rates. In general, biomimetic systems based on molecular motors and filaments have many potential applications in bionanotechnology, pharmacology, and medicine. During the next couple of years, we may witness the development of sorting devices for b...

Divergent mating systems and parental conflict as a barrier to hybridization in flowering plants

...lization and development, they provide barriers to hybridization and may facilitate speciation. ...

New evidence supports century-old theory of cancer spread

...stream oncology researchers to consider tumor cell hybridization with white blood cells as a major reason that cancer metastasizes or spreads to other parts of the body. "Cancer cells exhibit a remarkable number of traits normally attributed to white blood cells known as macrophages, including the ability to migr...

Detection of DNA on nanotubes offers new sensing, sequencing technologies

...emonstrated the optical detection of selective DNA hybridization on the surface of a nanotube," said Strano, who is...ptical detection of specific DNA sequences through hybridization with a complementary DNA probe has many potential applications in medicine, microbiology and environ...

Study suggests that publicly available genome data may contain small but significant errors

...NYU's School of Medicine. Using their micro-array hybridization method, which used flourescently labeled snippets ...at with appropriate experimental conditions, array hybridization data can be used to establish a physical distance between unique arrayed probes--a sequence of DNA w...

Pathway toward gene silencing described in plants

...scopy techniques known as RNA fluorescence in situ hybridization (RNA-FISH) and DNA-FISH, Washington University postdoctoral researcher Olga Pontes, Ph.D., was able to unravel where the eight team players are located and in what order events in the RNA-directed DNA methylation pathway transpire. Using antibodies...

Making a face: A new and earlier marker of neural crest development

...echnologies -- immunostaining of proteins, in situ hybridization and multiplex RT-PCR of mRNAs -- that formation of neural crest cells in chick embryos is independent of both mesoderm and neural tissues. They also identify, Pax7, as an early marker of neural crest formation and prove that its function is required ...

Ring-like formations in drying DNA drops could affect hybridization studies

...re sequentially deposited onto a glass surface for hybridization studies, the researchers report. "Without optimization of the wetting conditions, it is possible to miss all the DNA in the ring stain of a dried droplet, resulting in false negatives," Wong said. "We need to think of strategies to minimize this eff...

Study provides new understanding of spontaneous hybridization

Plant and animal breeders have long used hybridization to transfer useful traits between species. But doe...berg (Indiana University), explore how spontaneous hybridization ?known as adaptive trait introgression ?has a vital impact on adaptation and evolutionary diversific...

Sunflower speciation highlights roles for transposable elements in evolution

...n a finding that furthers our understanding of how hybridization may contribute to genome changes and the evolution...s--the origin of new species through inter-species hybridization may be facilitated by rapid reorganization of genomes. Previous work on three independently derived ...

New data from NIH lab confirms protocol to reverse type 1 diabetes in mice

... in the form of Y-chromosomal fluorescence in situ hybridization (FISH), in combination with insulin staining, to follow the fate of male spleen cells transplanted into female recipients. The female mice that received male donor cells consistently showed Y-chromosome-positive insulin-producing islet cells, indicat...

New technology used to construct the first map of structural variation in the human genome

...employed in a process known as comparative genomic hybridization (CGH), which involves tagging genomic DNA from two individuals and then co-hybridizing it to the array. Data from the array were assessed with a new algorithmic tool, called CNVfinder, which accurately and reliably identified CNVs in the human genom...

An advanced genetic diagnostic method for multiple myeloma

...tion of the disease. In addition, the technique of hybridization in situ with fluorescence allowed him to identify new recurrent genetic changes that are involved in the appearance of this pathology. A disease associated with old age Multiple myeloma is a disease which primarily affects persons above 60 years ...

Faster, low cost sequencing technologies needed to drive era of personalized medicine

...tered. "Traditional approaches to sequencing by hybridization are limited by the number of probes that can be placed on a chip," said Jian Gu, a research staff member in the Center for Applied NanoBioscience at the Biodesign Institute and co-leader of the project. By using nanoprinting techniques developed b...

Rapid, Low-Cost DNA Testing

...d costs $0.025. Step 2. Add gold colloid to the hybridization solution. This takes 10 seconds and costs $0.02. Step 3. Add salt to the solution. This takes 10 seconds and costs $0.01. Step 4. Measure photoluminescence. This takes 1 minute. It's as simple as that, yet nobody's ever done it before. The m...

Tibetan antelope slowly recovering, WCS says

...more endangered than the chiru, due to hunting and hybridization with domestic yak. The group saw an increase in wild asses, too, though they are persecuted by nomads who believe they compete with livestock for grass. Schaller noted that some nomadic communities living in the Chang Tang region have made conce...