exon in Biological News

New method provides panoramic view of protein-RNA interactions in living cells

... into some sort of message, all the spaces need to be removed; but if an exon is dropped, a different version of that protein, which could carry a very ... Nova2 binds to RNA, they could predict and directly observe whether an exon would be included or excluded in the final transcript, and which protein ...

DNA template could explain evolutionary shifts

... variation. "I think this is going to make people think very hard about copy number variation with respect to genome evolution, gene evolution and exon shuffling," said Dr. James R. Lupski ( http://www.bcm.edu/genetics/facultyaz/lupski.html ) , vice chair of molecular and human genetics at BCM and ...

Joining forces to improve lung cancer treatment

... with dose-per-fraction escalation for treatment of stage III non-small-cell lung cancer (Poland) Confirmation that EGFR mutations (deletion in exon 19, missense L858R) are the most important predictors of sensitivity to tyrosine-kinase inhibitor targeted therapies (Italy) Barbara Zolty from ...

Boehringer Ingelheim uses Genomatix' Next Generation Sequencing data analysis systems

... and peak finding, and performs automated binding pattern identification. For RNAseq experiments, normalized expression values are calculated at the exon and transcript level. A special GenomeThesaurus is also provided for potential splice junctions, which allows for splice junction analysis and ...

Genomatix joins Illumina-Connect program

... and peak finding, and performs automated binding pattern identification. For RNAseq experiments, normalized expression values are calculated at the exon and transcript level. A special GenomeThesaurus is also provided for potential splice junctions, which allows for splice junction analysis and ...

Human genes sing different tunes in different tissues

... tissue-dependent. Certain protein isoforms that are common in heart tissue, for example, might be very rare in brain tissue, so that the alternative exon functions like a molecular switch. Scientists who study splicing have a general idea of how tissue-specificity may be achieved, but they have much ...

Deep sequencing study reveals new insights into human transcriptome

... under study. In addition, a global survey of mRNA splicing events identified 94,241 splice junctions, of which 4,096 are novel, and showed that exon skipping is the most prevalent form of alternative splicing. Details are presented in the Science report of Sultan et al. "A Global View of Gene ...

Researchers discover critical detail of cellular defense against genetic mistakes

... NMD quality review depends on the physical spacing of proteins bound to the mRNA chain. If a stop reading signal occurs too far ahead of the final exon in the chain, as marked by an exon-exon junction complex (EJC), the cell concludes that the stop codon has mistakenly fallen in the middle of a set ...

Living view in animals shows how cells decide to make proteins

... intronic silencers were chopping out the IIIb exon -- and if so, in which tissues and organs -- or ... in most tissues made the same decision to silence exon IIIb, but the cells used a variety of silencers ... For example, a cell’s decision to include exon IIIb is critical because the exon’s presence or ...

RNA map provides first comprehensive understanding of alternative splicing

... different mRNAs, and thus different proteins. The exon can be spliced in or out in a binary, ... action on regulating whether the alternative exon is spliced in or out. The researchers ... inhibited by Nova and half were enhanced in their exon use by Nova, and every one very cleanly fit the ...

'Smart' genetic therapy helps the body to heal itself

... an anti-sense drug that induces the skipping of exon 51 from the DMD gene. Six Dutch DMD patients ... that are correctable by skipping that particular exon will take part. This human trial is being ... to study optimal dose regimes, and further study exon skipping levels. This study found no clinical ...

Modification of program enables prediction of gene transcription

... first splice occurs thereby defining the first exon of the gene. The modification to the gene ... start site (TSS) and the complete first exon in both the human and fruit fly genomes. The ... game to a multiple choice question ?is it a UTR exon or a protein-coding exon? These kinds of ...

RNA splicing occurs in nerve-cell dendrites

... splicing works by cutting out introns and merging together the remaining exon pieces, resulting in an mRNA capable of being translated into a specific ... and reconnected. Specifically, splicing removes pieces of intron and exon regions from the RNA, with the resulting spliced RNA often being made into ...

Genomatix Microarray Analysis Pipeline achieves Affymetrix GeneChip compatible?status

... users, including analysis of data from Affymetrix whole-genome tiling and exon arrays. "We are proud of having met the high standards of Affymetrix ... Genomatix. "Especially in conjunction with the new Affymetrix tiling and exon array technology, I expect that our tools will drive far greater ...

NIH neuroscience microarray consortium launches high-throughput genotyping services

... Ambion mirVana arrays, and Operon oligo arrays; Yale offers expression profiling and genotyping on the Affymetrix platform and uses the Affymetrix exon arrays to look at splice variants, in addition to the new Illumina expression and genotyping capabilities. The principal investigator at Yale, ...

Study provides insight into cellular defenses against genetic mutation

... regulatory action of NMD depends on the physical spacing of molecules in the RNA chain. If a stop reading signal occurs too far ahead of the final exon in the chain, as marked by an exon-exon junction complex (EJC), the cell concludes that the stop codon has mistakenly fallen in the middle of a set ...

Reversal of role for a viral protein associated with the development of lymphoma

... found at the end of an intron. Such a unique pairing allows a ratchetting point to function sequentially as an acceptor for splicing to an upstream exon and then as a donor for splicing to the next downstream ratchetting point or exon. As the process goes from ratchetting point to ratchetting point, ...

Carnegie Mellon University research reveals how cells process large genes

... found at the end of an intron. Such a unique pairing allows a ratchetting point to function sequentially as an acceptor for splicing to an upstream exon and then as a donor for splicing to the next downstream ratchetting point or exon. As the process goes from ratchetting point to ratchetting point, ...