Researchers create new class of fluorescent dyes to detect reactive oxygen species in vivo
... experimental results showing the ability of the dyes
to detect reactive oxygen species in cells, ...
The researchers have created six hydrocyanine dyes
to date hydro-Cy3, hydro-Cy5, hydro-Cy7, ... potentially 40 probes that could be created. The dyes
vary in their ability to detect intracellular or ...
DNA fingerprinting simplified
... electrophoresis to anyone. Popular brands of food dyes
(red, blue, yellow, and green) purchased at local ... into blue and yellow) and some apparently single dyes
often have extra surprise components.
... the article, much of the experimentation with the dyes
and helped develop the dye-based forensic ...
Ancient diatoms lead to new technology for solar energy
... photons bounce around like they were in a pinball machine, striking these dyes
and producing electricity. This technology may be slightly more expensive ... and inexpensive materials.
"Conventional thin-film, photo-synthesizing dyes
also take photons from sunlight and transfer it to titanium dioxide, ...
Basic guides to PCR, labeling neurons featured in Cold Spring Harbor Protocols
... cultured neurons, brain slices, and in vivo preparations. A wide range of dyes
can be loaded using this method, including probes for morphological reconstruction, ion-sensitive indicator dyes
for monitoring second-messenger cascades, and dye-labeled proteins for ...
American Chemical Society's Weekly PressPac -- May 28, 2008
... nylon, silk and other fabrics, scientists in California are reporting. The dyes
from Mother Natures palate also have an anti-bacterial effect that could ... her adviser Gang Sun and colleagues point out that conventional dyes
and pigments used in clothing have several drawbacks. Many are made from ...
Stem-cell transplantation improves muscles in MD animal model, UT Southwestern researchers report
... Dr. Perlingeiro said.
The researchers then began using fluorescent dyes
to sort cells depending on whether some surface markers were turned on ... into the animals hind-limb muscles. After a month, the fluorescent dyes
showed that the cells had deeply penetrated the muscle, an indication that ...
'Microfluidic palette' may paint clearer picture of biological processes
... gradients in the chamber can be maintained virtually indefinitely.
To demonstrate how the microfluidic palette works, the NIST researchers inject dyes
of the three primary colorsred, yellow and blueseparately into the three inlets of the system. For each dye, an independent gradient forms that ...
New technique can fast-track better ionic liquids for biomass pre-treatments
... contain molecules which fluoresce when excited by the right light and this fluorescence can compete with the signals obtained from the fluorophore dyes
or markers used to label biological objects of interest. Simmons and Singh have turned this "nuisance" into an effective tool. Using ...
Researchers observe single protein dimers wavering between two symmetrically opposed structures
... (smFRET), a tool they have helped develop. This technique, which Deniz calls a "molecular ruler," measures light emitted from two fluorescent dyes
that are attached to amino acids in the protein. The color of the measured light provides information about molecular distances that reveals a ...
Changing climate likely to make 'super weed' even more powerful
... gallic acid to kill off plants and take over new turf. Gallic acid, also known as 3,4,5-trihydroxybenzoic acid, is used for tanning leather, making dyes
and inks, and formulating astringents, among other applications.
In this research, the scientists found that the gallic acid released by ...
Scripps research scientists 'watch' as individual alpha-synuclein proteins change shape
... protein folds when it binds to different molecules. This technique, which Deniz calls a "molecular ruler," measures light emitted from fluorescent dyes
that are attached to amino acids in the protein. The measured light provides information about molecular distances, hence revealing the protein's ...
Spun-sugar fibers spawn sweet technique for nerve repair
... an atomic force microscope, was used to obtain images of the hollow tubes and pores in the walls of the tubules. Other images using fluorescent dyes
revealed the nerve cell alignment along the tubes.
The work was done using cell cultures in petri dishes, but ongoing work focuses on implanting ...
No longer a gray area: Our hair bleaches itself as we grow older
... speculate that a similar breakdown in the skin could be the root cause of vitiligo.
"As any blue-haired lady will attest, sometimes hair dyes
don't quite work as anticipated," Weissmann added. "This study is a prime example of how basic research in biology can benefit us in ways never ...
American Chemical Society's Weekly PressPac -- Jan. 28, 2009
... Karlheinze Peter and colleagues note that the most reliable test for diagnosing CAD is angiography, an invasive test in which doctors inject special dyes
into the body to visualize, via X-rays, fatty plaque deposits in the arteries of the heart. However, the technique is invasive, expensive, ...
Unusual microbial ropes grow slowly in cave lake
... rope using three dyes, one for DNA, one for bacteria and one for archaea, show very little activity in the bacteria or archaea, probably because the dyes
highlight ribosomes and they only exist in a cell when it is actively metabolizing.
The researchers, who include Macalady; Daniel S. Jones and ...
Moving new technologies from the lab to the marketplace
... and electronics materials.
Sergiu Gorun, associate professor of chemistry, received an award to further develop his phthalocyanine dyes. The dyes
have the unique characteristic of absorbing heat, allowing visible light to pass through the polymer thus opening up an array of new civilian and ...
A green future for scrap iron
... 76 to 87 percent. Improvements were also recorded with the removals of nitrogen (13 to 85 percent), phosphorus (44 to 64 percent), and colors and dyes
(52 to 80 percent).
"Before this project," says Ma, "few people believed scrap iron could work in a wastewater treatment plant. We have developed ...
Sensitive nanowire disease detectors made by Yale scientists
... we can hear the few above the many."
According to the authors, this level of sensitivity and specificity is unprecedented in a system that uses no dyes
or radioactivity. Beyond its sensitivity, they say, the beauty of this detection system is in its speed producing results in seconds and its ...
Can genetic information be controlled by light?
... diagnostic method whereby laser light could be used to directly recognise certain genetic sequences without, for example, having to mark the DNA with dyes
as in the method used at present".
One might also envisage linking the photophysical properties to genetic characteristics. When these mechanisms ...
Disinfectants can make bacteria resistant to treatment
... that mutants that make more efflux pumps than normal were produced.
"We found that exposure to low concentrations of a variety of biocides and dyes
resulted in the appearance of resistant mutants," said Dr Kaatz. "The number of efflux pumps in the bacteria increased. Because the efflux pumps can ...
Colorful spy tactics track live cells supporting cancerous tumors
... against the tumor, while others help the tumor grow and spread.
In order to watch the activity of these cells, researchers injected fluorescent dyes
near tumors in mouse models of breast cancer which also expressed fluorescently tagged cells A specially designed microscope allowed live imaging ...
From humming fish to Puccini: Vocal communication evolved with ancient species
... before the first animals ventured onto dry land.
The research is published in the July 18 issue of the journal Science .
Bass used fluorescent dyes
to identify distinct groups of neurons in the brains of the larvae of midshipman fish, a species known for the loud humming sounds adult males ...
Scripps Research Institute awarded patent for remarkable chemical technology
... AIDS, nicotine addiction, and other conditions. The reactions' gluing powers have also proven extremely effective at binding fluorescent alkyne dyes
to proteins and other biological components, which allows researchers to observe how they behave in cells and what roles they may play in diseases. ...
Detecting dangerous chemicals with lasers, exploring the brain's circuitry with light and more
... use optical probes that can detect neuronal activity with light, but these methods often require labeling neural cells with electrically-sensitive dyes
that may be toxic to neurons.
Now Jiayi Zhang, Tolga Atay, and Arto Nurmikko at Brown University have created a new type of dye-free optical probe ...
American Chemical Society's Weekly PressPac -- March 19, 2008
... laboratory science conference held earlier this month in New Orleans. These new tools include a highly-sensitive method for identifying the specific dyes
used to color acrylics, cotton, nylon, and other types of fibers, a technique that could help distinguish between fibers that appear similar. Other ...
ETH Zurich researchers develop antibody test
... in varying concentrations. When blood serum is then administered to such a pad, possible antibodies specifically bind to certain sugar molecules. dyes
then reveal to which GPIs the antibodies have attached themselves.
Help for infants
Thanks to the information obtained from the chip, ...
Biomagnetics developed for use in new breast cancer tests
... of the London Centre for Nanotechnology and the UCL Department of Physics & Astronomy. Until now, pathologists had to stain tissue samples with brown dyes
to help them determine whether they were normal or cancerous. In terms of streamlining the process, the main problem is that all of the results are ...
Drawing nanoscale features the fast and easy way
... produced in the material.
By changing the chemistry of the polymer, weve shown that we can selectively attach new substances, like metal ions or dyes
to the patterned regions of the film in order to greatly increase the techniques functionality, said Seth Marder, professor in Techs School of ...
Allergy to hair dye increasing
... may occur.
More than two thirds of hair dyes
currently contain para-phenylenediamine (PPD) and ... a serious problem that it was banned from hair dyes
in Germany, France, and Sweden.
Current ... to comprise up to 6% of the constituents of hair dyes
on the consumer market, but no satisfactory or ...
The biggest bug in gut discomfort
... all" To check, the researchers fed traceable dyes
to infected cells. Taken up into the endocytic pathway, the fluorescent dyes
co-localized with control bacteria known to traffic to lysosomes, yet surprisingly the dyes
were unable to enter vacuoles containing C. ...
Connect the Quantum Dots
... dots can one day replace conventional organic dyes
in biomedical applications.
By using the ... samples thus replacing conventional organic dyes
in a diversity of imaging and sensing ... research but rely on the fluorescence of organic dyes
which have limited luminescent properties. ...
Color-blind method opens new doors in DNA sequencing
... is done with one laser and optics to separate the dyes
into the four colors, blue, green, yellow, and ... is that the color of light emitted by the dyes
is similar. Even with complex computer programs ... the signals, this "cross-talk" between the dyes
results in subtle variations that can cause ...
Lab-on-a-chip could speed up treatment of drug-resistant pneumonia
... to certain strains of bacteria, and mark them with fluorescent dyes
of different colors. The dyes
color-code cells from known strains. A microscope monitors the viable ...
With record resolution and sensitivity, tool images how life organizes in a cell membrane
... the chemical structure of the molecules that they label. In contrast, the dyes
commonly used in biological studies for labeling change the structure of the molecules to which they attach and can alter their behavior. Moreover, dyes
do not allow scientists to analyze membrane compositions, because ...
One gene links newborn neurons with those that die in diseases such as Alzheimer's
... pathway. In order to label the two cell types, the scientists injected dyes
of different colors, one green and one red, into the brain regions where the neurons project. The dyes
then traveled back to the bodies of the cells in the high vocal center. As ...
Robot-based system developed at Carnegie Mellon detects life in Chile's Atacama desert
... such as cyanobacteria in lichens, and fluorescent signals from a set of dyes
designed to light up only when they bind to one of the following ?nucleic ... said Waggoner.
"To avoid this problem, we designed our system to excite dyes
with high intensity flashes of light. The camera only opens during those ...
Cellular message movement captured on video
... that paxillin serves as a docking station for a variety of signaling and structural proteins, they have been limited to visualizing paxillin with dyes
that stain cells rigidly attached to microscope slides. Those snapshots of fixed cells can’t reveal paxillin movement.
“We can now see the ...
Cancer stem cells similar to normal stem cells can thwart anti-cancer agents
... assistant professor of surgery and pharmaceutical sciences, University of Pittsburgh Schools of Medicine and Pharmacy, used cell surface markers and dyes
to identify cancer stem cells as well as normal adult stem cells and their progeny in samples obtained from normal lung and lung cancer tissue ...
3-D ultrasound scanner provides in-depth view of the brain
... brain, as is required in surgeries to drain cerebrospinal fluid and relieve pressure on the brain. In a second animal, the researchers made use of dyes
to enhance ultrasound images of blood vessels in the brain.
The new advance is the latest in a series of developments Smith's team has made ...