dyes

Color-blind method opens new doors in DNA sequencing

... is done with one laser and optics to separate the dyes into the four colors, blue, green, yellow, and red...echnique is that the color of light emitted by the dyes is similar. Even with complex computer programs to assist in deciphering the signals, this "cross-ta...

Clam embryo study shows pollutant mixture adversely affects nerve cell development

...investigators used a combination of antibodies and dyes toshow the presence of neurotransmitters in single neurons. "We'veactually taken superlative three-dimensional pictures where we go rightthrough the embryo," says Reinisch. The sequence of images yields athree-dimensional representation of the embryo...

Study identifies predictors of HIV drug resistance in patients beginning triple therapy

...investigators used a combination of antibodies and dyes toshow the presence of neurotransmitters in single neurons. "We'veactually taken superlative three-dimensional pictures where we go rightthrough the embryo," says Reinisch. The sequence of images yields athree-dimensional representation of the embryo...

Nano-Probes Allow an Inside Look at Cell Nuclei

...t, conventional labels such as organic fluorescent dyes and green fluorescent proteins only fluoresce for a few minutes at a high resolution. These labels are also either toxic to cells or difficult to construct and manipulate. In the future, they hope to tailor quantum dots to track specific chemical re...

Robot-based system developed at Carnegie Mellon detects life in Chile's Atacama desert

... in lichens, and fluorescent signals from a set of dyes designed to light up only when they bind to one of...oid this problem, we designed our system to excite dyes with high intensity flashes of light. The camera only opens during those flashes, so we are able to ...

Revolutionary nanotechnology illuminates brain cells at work

...ion, the same response occurs with two fluorescent dyes that have overlapping, but slightly different colors–in this case cyan and yellow versions of GFP. The cyan is excited and, if the distance between the colored proteins changes, more or less energy is transferred to the yellow protein. In this study,...

Study: 'homemade' gene expression technology unreliable

...s of DNA or RNA molecules labeled with fluorescent dyes on the slide, and examining whether the molecules that bind to a particular gene light up when viewed with a special scanner. By interrogating thousands of genes at once, scientists can quickly pinpoint genes affected by drugs being tested to treat ...

One gene links newborn neurons with those that die in diseases such as Alzheimer's

... label the two cell types, the scientists injected dyes of different colors, one green and one red, into the brain regions where the neurons project. The dyes then traveled back to the bodies of the cells in the high vocal center. As a result, replaceable and...

Brown-Harvard team solves mobile DNA's surgical sleight-of-hand

...nces, Radman-Livaja tagged bits of lambda DNA with dyes that allowed her to draw up a map of this viral DNA during insertion. "It was gratifying to see that two totally different experimental approaches give the same picture," Radman-Livaja said. "It means we have a very solid foundation for the next ste...

Virginia Tech group adds tools to DNA-targeted anti-cancer drugs

...n practice in the medical field to use luminescent dyes to study cells, so pathology labs will have the equipment needed to monitor drug delivery. Brewer is giving her invited talk, "Applications of mixed-metal supramolecular complexes in the development of chemotherapeutic and photodynamic therapy agent...

Bad aftertaste? New sensory on/off switch may 'cure' bane of artificial sweetener search

...n practice in the medical field to use luminescent dyes to study cells, so pathology labs will have the equipment needed to monitor drug delivery. Brewer is giving her invited talk, "Applications of mixed-metal supramolecular complexes in the development of chemotherapeutic and photodynamic therapy agent...

Several minute intermediate stage in virus-cell fusion discovered; opportunity for drug development

...nometers across, by labeling them with fluorescent dyes and recording at one frame every 7 seconds. (For comparison purposes, a human hair is 80,000 nanometers thick.) ASLV is a useful model for several viruses, including Ebola, the 'flu, and measles, as well as HIV. "This system is especially well suite...

A step toward the $1,000 personal genome using readily available lab equipment

...hrough inexpensive optics. One of four fluorescent dyes corresponding to the four DNA bases binds at a specific location on the genetic sequence, depending on which DNA base is present. The fragment then shines with one of the four colors, revealing the identity of the base. Recording the color data from ...

Team led by Carnegie Mellon University scientist finds first evidence of a living memory trace

...fferent glomeruli, and imaged their activity using dyes sensitive to the opening and closing of calcium channels on neurons. The scientists created an imaging grid across the glomeruli and measured the ratio of fluorescence versus nonfluorescence over time. Galán then used advanced mathematical technique...

Olfactory system detects pheromones that control reproduction

...f neurons used techniques that depended heavily on dyes that could not label specific populations of neurons. These experiments suggested that neurons of the vomeronasal system are directly connected with LHRH neurons, and thus carry the modulatory effect of pheromonal cues on LHRH release. But because cl...

Value of services performed by insects tops $57 billion in US

...lihood of forest fires); producing honey, shellac, dyes and other products; being used in medicine or as a source of food for animals other than those used in hunting, fishing and birding; and providing a direct source of food for humans. Based on their analysis, Losey and Vaughan call for greater invest...

Connect the Quantum Dots

...ntum dots can one day replace conventional organic dyes in biomedical applications. By using the unique...ogical samples thus replacing conventional organic dyes in a diversity of imaging and sensing applications." Fluorescence microscopy and spectroscopy have ...

Recombination protein dynamics observed with single monomer resolution

...researchers then measure the brightness of the two dyes simultaneously. The changing ratio of the two intensities indicates the relative movement of the two dyes, and therefore the motion of the molecule or its change in size. The technique revealed intricate details of how RecA nucleates to form a filam...

Scientists solve sour taste proteins

...d the cells with two calcium-sensitive fluorescent dyes -- one that glowed green when the calcium concentration was high, the other that glowed red when the concentration was low. When they added sour-tasting acids to the cells, the ion channels went from closed to open, enabling calcium ions to flow in,...

With record resolution and sensitivity, tool images how life organizes in a cell membrane

...of the molecules that they label. In contrast, the dyes commonly used in biological studies for labeling c...hey attach and can alter their behavior. Moreover, dyes do not allow scientists to analyze membrane compositions, because scientists see only what they've l...

The biggest bug in gut discomfort

...after all" To check, the researchers fed traceable dyes to infected cells. Taken up into the endocytic pathway, the fluorescent dyes co-localized with control bacteria known to traffic to lysosomes, yet surprisingly the dyes were una...

Lab-on-a-chip could speed up treatment of drug-resistant pneumonia

...trains of bacteria, and mark them with fluorescent dyes of different colors. The dyes color-code cells from known strains. A microscope monitors the viable cells -- those that are still ...

Brown scientists map structure of DNA-doctoring protein complex

...lexes in a gel. He tagged the DNA with fluorescent dyes and purified the proteins, placing them in a gel that was then shot through with light. Sun measured the wavelengths of light as they bounced between the molecules of dye. Those measurements were then fed into a special software program created by Da...

Walking molecule now carries packages

...t is also the parent substance of a large class of dyes and pigments. Its chemical formula is C14H8O2. The UCR study used a scanning tunneling microscope in Bartels's laboratory that gives a precise picture of individual molecules. Experiments took place on a highly polished copper surface, cleaned so t...

No carrier necessary: This drug delivers itself

... nanocrystals as a delivery method for hydrophobic dyes in bioimaging applications, another promising use for nanocrystals that they continue to pursue. Further in vivo studies with HPPH nanocrystals are being conducted by scientists at UB and RPCI, including Pandey and Allan R. Oseroff, M.D., Ph.D., c...

Biologists prove critical step in membrane fusion

...ut the contents remain separate. Using fluorescent dyes and a high-resolution microscope, the researchers show that hemifusion is surprisingly stable in live cells. "The novelty of these results is that a live cell can maintain a hemifused state for hours, days, even months," said Julian Wong, a postdo...

Allergy to hair dye increasing

...welling may occur. More than two thirds of hair dyes currently contain para-phenylenediamine (PPD) and ...uch a serious problem that it was banned from hair dyes in Germany, France, and Sweden. Current European Union legislation allows PPD to comprise up to 6...

Using nanomagnets to enhance medical imaging

... as the composition was varied. The injectable dyes currently used as MRI contrast agents are of two types. Magnetic ions, which alter the nuclear properties of hydrogen in water, offer the advantage of consistent identical design but provide low contrast. The second category encompasses particles of ...

Sandia researchers take new approach to studying how cells respond to pathogens

...uorescent molecules, essentially colored dyes. The dyes range from green to red and give researchers the opportunity to track proteins and see, for example, the dynamic cellular production of proteins or protein-binding processes inside or on the surface of the cells. The team is developing one platfor...

3-D ultrasound scanner provides in-depth view of the brain

...n. In a second animal, the researchers made use of dyes to enhance ultrasound images of blood vessels in the brain. The new advance is the latest in a series of developments Smith's team has made since developing the first 3-D ultrasound scanner in 1987 for imaging the heart from outside the body. ...

Cancer stem cells similar to normal stem cells can thwart anti-cancer agents

...dicine and Pharmacy, used cell surface markers and dyes to identify cancer stem cells as well as normal adult stem cells and their progeny in samples obtained from normal lung and lung cancer tissue samples. The researchers identified a very small, rare set of resting cancer stem cells in the lung cancer ...

Cellular message movement captured on video

...hey have been limited to visualizing paxillin with dyes that stain cells rigidly attached to microscope slides. Those snapshots of fixed cells can’t reveal paxillin movement. “We can now see the dynamics of how paxillin and other proteins move inside the cell with new fluorescent labels and live-cell v...