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Vitro in Biological News

Attitudes towards assisted reproduction and preimplantation genetic diagnosis

... reproduction techniques in general and in vitro fertilization in particular as firmly acceptable ... countries). However, this strong approval for in vitro fertilization dissipates in other scenarios such ... of fertility: 1) use assisted reproduction or in vitro fertilization techniques, 2) adopt a child, or 3) ...

Spotlight on cosmetic ingredients conference

... The event, called Spotlight on Ingredients: In vitro Alternatives Forum, will take place on Thursday ... behind them, and how to move forward with in vitro testing programs. Dr. Rodger Curren, the president of event co-sponsor the Institute for In vitro Sciences, says, "We intend that participants will ...

Study links nicotine with breast cancer growth and spread

... Medical Center. Through a series of in vitro tests Chen and her team of researchers determined ... migrated to the lungs. From in vivo and in vitro studies, it indicates that nicotine is not a ... factors to enable tumorigenesis. "In vitro and in vivo tests showed that no metastasis ...

It is important to demonstrate the influence of the microenvironment in the process of metastasis

... University of the Basque Country (UPV/EHU), an in vitro culture model of human colon cancer was created ... as metastasis in the liver of patients. This in vitro culture model provides a simple tool for the ... study aimed, on the one hand, to create an in vitro culture model of human colon cancer that would ...

Chromosomal problems affect nearly all human embryos

... must be present in all human IVF-ICSI embryos. "Although in vitro culture conditions are known to have a limited influence on the rate of ... embryos, it is probable that the chromosome instability observed in vitro also occurs in spontaneous pregnancies since, at most, 30% of human ...

Vitamin D levels linked to asthma severity

... Journal of Respiratory and Critical Care Medicine . While previous in vitro studies have suggested that vitamin D may affect how airway cells respond ... "This study also provides epidemiological support for a growing body of in vitro evidence that vitamin D insufficiency may worsen asthma severity, and we ...

Peregrine's PS-targeting antibodies highlighted in AACR Annual Meeting studies

... The studies also demonstrated the anti-tumor potential of PGN635 in vitro and in a number of animal cancer models. "Data from our experiments has ... cells, important antigen-presenting cells of the immune system. In the in vitro studies, immature dendritic cells cultured in the presence of PGN632 ...

Scripps scientists develop first examples of RNA that replicates itself indefinitely

... is, result in new copies of RNA that also could copy themselves. In vitro Evolution A few years after Tracey Lincoln arrived at Scripps Research ... Research. Their work began with a method of forced adaptation known as in vitro evolution. The goal was to take one of the RNA enzymes already developed ...

Building the blood-brain barrier

... the BBB matured. Activation of the Wnt signaling pathway in vivo and in vitro promoted BBB development, and inactivation prevented it. In vitro increasing Wnt signaling also strengthened junctions between non-brain ...

Rutgers scholar authors definitive biography of reproductive medicine pioneer

... CAMDEN As Louise Brown the first baby conceived by in vitro fertilization celebrates her 30th birthday in 2008, a new book coauthored ... medicine. Rock was the first researcher to fertilize a human egg in vitro in the 1940s and the co-developer of the first oral contraceptive a decade ...

Scientists form alliance to develop nanotoxicology protocols

... researchers and foster experiments to evaluate correlations between in vitro testing and toxicological interactions in mammals and aquatic animals. ... interactions of nanomaterials and improve correlations between in vitro testing and outcomes in animals, humans and the environment. ...

American Chemical Society's Weekly PressPac -- Aug. 27, 2008

... #1 FOR IMMEDIATE RELEASE A "lab on a chip" to improve success of in vitro fertilization Analytical Chemistry In a finding that could boost the success rate of in vitro fertilization (IVF), researchers report development of a tiny "lab on a ...

Human embryonic stem cells developed from 4-cell embryo; world first may lessen ethical concerns

... stage embryos were split into 12 single blastomeres and allowed to grow in vitro to produce twelve morulas that were cultured in the conventional way for ... the derivation of a personal hESC line that could be used to generate in vitro hematopoietic stem cells as an additional source of hematopietic stem ...

Oosight microscope enables embryonic stem cell breakthrough

... in clinical settings to evaluate the quality of human eggs prior to in vitro fertilization procedures, and thus reduce the number of nonviable embryos ... And it was David Keefes vision that the LC-PolScope could be used for in vitro fertilization or for enucleation, since it can be used to visualize the ...

Proteins pack tighter in crowded native state

... said. "This offers the first observed evidence -- both in silico and in vitro -- for structural effects on proteins in the native state." To find out ... Wittung-Stafshede said the group is following up with similar in vitro studies of several other proteins. The flavodoxin results and preliminary ...

Penn Veterinary Medicine report new strategy to create genetically-modified animals

... experimentally induced cryptorchid donor testes that were then exposed in vitro to AAV vectors carrying a green fluorescent protein transgene and ... testes and produced transgenic sperm. When semen was used for in vitro fertilization, 10 percent of embryos were transgenic. Initially, the ...

Paracetamol, one of most used analgesics, could slow down bone growth

... that taking paracetamol slows down bone growth, as has been proved by in vitro studies. Author of this work is Olga Garca Mart nez, and her analysis ... be used instead. Results of her work can not be confirmed in humans but in vitro research shows without a question that paracetamol slows down bone ...

JCI table of contents: Aug. 16, 2007

... Leonid Metelitsa and colleague from Childrens Hospital Los Angeles show in vitro that expression of a protein known as MYCN, which has been associated with ... with tumoral calcinosis has a mutation in both copies of her KL gene. In vitro analysis revealed that the KL protein resulting from this mutation was ...

K-State researcher, collaborators study virulence of pandemic H1N1 virus

... is very critical now that an agent causing a pandemic flu in humans most likely originated in animal populations." At K-State, Richt is leading in vitro research to develop better testing tools, creating a "diagnostic arsenal" if H1N1 were to spread to swine populations. Richt said they are developing ...

Skin-like tissue developed from human embryonic stem cells

... how hESC can be developed into the multilayer tissues similar to those that line the gums, cheeks, lips, and other areas in the mouth. We used in vitro tissue engineering techniques to produce skin-like tissues that mimic the lining tissues found in the oral cavity," said Garlick. Using a ...

ICSI or IVF: Babies born from frozen embryos do just as well

... study reported none of the specific abnormalities linked to male factor infertility, medications or other environmental triggers such as extended in vitro culture, which have been reported by other studies," she said. "When you think about it, the reproductive medical field has created a new ...

Female human embryos adjust the balance of X chromosomes before implantation

... present also in human embryos. This means that dosage compensation is present before implantation and this could have possible implications for in vitro culture such as during IVF treatment. Furthermore, our research shows that X chromosome inactivation in humans is not very different from other ...

A breath mint made from... coffee?

... Elite coffee, Landwer Turkish coffee, and Taster's Choice. Prof. Rosenberg expected to demonstrate the malodor-causing effect of coffee in an in vitro saliva assay developed by Dr. Sarit Levitan in his laboratory. To his surprise, the extracts had the opposite effect. "The lesson we learned here ...

Peripheral nerve repair with fat precursor cells led to wider nerves and less muscle atrophy

... the journal Cell Transplantation (18:2). Adipose precursor cells, said Marra and co-authors, have demonstrated an ability to differentiate in vitro into cartilage (chondrogenic), bone (osteogenic), fat (adipogenic) and muscle (myogenic) cell types. Control groups for this study included those ...

UCLA cancer researchers develop model that may help identify cancer stem cells

... associated with cancer stem cells and found signatures for most of those markers in the cells they grew. "We were able to establish cultures in vitro with high efficiency using the novel strategy that utilized an autologous tumor microenvironment," the study states. "In this primary culture model, ...

Universitat Autonoma de Barcelona researchers first to clone mice in Spain

... no inhibiting substance in the third group. The three mice in this case were cloned using the first (Cloe and Clona) and second (Cleo) inhibitors. In vitro experiments already pointed to improvements in the development of cloned embryos using inhibitors. However, scientists must wait until the end of the ...

American Society for Microbiology honors Deborah T. Hung

... in Science. Currently, Dr. Hung's work focuses on tuberculosis (TB). She has already developed screens to identify small molecules that probe in vitro TB physiology and TB infection of macrophages. Her goal is to understand its persistent state by developing chemicals that can manipulate it and by ...

Microtest Labs Adds Dissolution Testing to its Suite of Analytical and Stability Testing Services

... formulations, different processes, analysis of competitor's samples) To estimate release and absorption rates in humans after an in vitro -- in vivo correlation is established Dissolution is a sample preparation technique. Samples still need to be analyzed separately, ...

American Chemical Society's weekly PressPac -- May 6, 2009

... EU regulations are often adopted in other countries," the article notes. As a result, cosmetic makers are evaluating safety with so-called in vitro or "test tube" testing, simulations of cosmetic effects with computers, and safety information in existing databases. Some manufacturers express ...

Novel antibody prevents infection by hepatitis C virus

... recognize and bind to the HCV's outer coat, known as the glycoprotein. Once they found human antibodies that looked promising, they evaluated in vitro the ability of those antibodies to neutralize the virus and selected a lead candidate antibody for further characterization. Collaborative work with ...

SUMO protein guides chromatin remodeler to suppress genes

... BOSTON (April 27, 2009) In an in vitro study, led by Grace Gill, PhD, Tufts University School of Medicine, researchers discovered how a protein called SUMO (Small Ubiquitin-related ...

Redefining what it means to be a prion

... genome," says Simon Alberti, a Lindquist postdoctoral researcher and co-author of the paper. "And the results from work in yeast cells, and the in vitro work overlapped and supported each other very nicely." Alberti and Halfmann hypothesize that prions in yeast prepare individual organisms for ...

Brain building: Study shows brain growth tied to cell division in mouse embryos

... distribution of a spindle-orienting factor that in normal cells distributes asymmetrically. Such similarities between Vangl2-lacking cells in vitro and in vivo will facilitate ongoing studies of the PCP pathway in neurogenesis. ...

CHIs 5th Biomarker World Congress: Signature Event with Cutting-Edge Agenda

... Foernzler, Ph.D., Biomarker-Experimental Medicine Leader, F. Hoffmann-La Roche AG Alberto Gutierrez, Ph.D., Deputy Director, Office of In vitro Diagnostic Device Evaluation and Safety (OIVD), U.S. Food and Drug Administration Yiwu He, Ph.D., Senior Program Officer, Global Health ...

To fight drug addiction, UB researchers target the brain with nanoparticles

... short RNA molecules in the brain to "silence" or turn off a gene that plays a critical role in many kinds of drug addiction. The UB team's in vitro findings were published online the week of March 23 in the Proceedings of the National Academy of Sciences . "These findings mean that in the ...

Time (and PPAR-beta/delta) heals all wounds

... dermal fibroblasts in a gel of extracellular matrix proteins. Keratinocytes are seeded on top of this gel and the two cell types develop into an in vitro version of skin that looks remarkably like the real thing. The fibroblasts and keratinocytes can therefore be manipulated separatelyknocking down or ...

A new way to assemble cells into 3-D microtissues

... CA Scientists at the U.S. Department of Energy's Lawrence Berkeley National Laboratory can now control how cells connect with one another in vitro and assemble themselves into three-dimensional, multicellular microtissues. The researchers demonstrated their method by constructing a tailor-made ...

Ex vivo neural stem cell expansion

... stem cell self-renewal. Overexpression of Bmi-1 in adult and embryonic forebrain neural progenitor cells increases stem cell self-renewal both in vitro and in vivo. They furthermore show that Bmi-1-mediated self-renewal is dependent upon repression of p21 by the forebrain-specific transcription ...

UCLA stem cells scientists make electrically active motor neurons from iPS cells

... of human embryonic stem cell and human iPS cell technology is to be able to generate relevant cell types to enable the repair of tissue damage and in vitro modeling of human disease processes," the study states. "Here, we demonstrate the successful generation of electrically active motor neurons from ...

Reducing animal experiments through top-class research

... in a very topical field of research that is likely to be of increasing importance both for medicine as well as for human health in general. The in vitro immortalisation assay (IVIM) method developed by Professor Baum and his team helps improve the chances of success for gene therapy. This aims to ...
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