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Hybridization in Biological News

Microarray analysis improves prenatal diagnosis

... BCM, described use of array comparative genomic hybridization to analyze samples taken during amniocentesis or ... she said. Array comparative genomic hybridization provides the tools to scan fetal DNA quickly and ... In two cases the array comparative genome hybridization identified disorders that would have been missed ...

UT researcher sheds new light on hybrid animals

... the hybrids to be genetically impure and see hybridization as accelerating extinction." It is not yet ... discoveries place the work on the leading edge of hybridization studies. "We're right at the front in thinking ... in recent months that have explored the issue of hybridization in butterflies. ...

UT researcher sheds new light on hybrid animals

... the hybrids to be genetically impure and see hybridization as accelerating extinction." It is not yet ... discoveries place the work on the leading edge of hybridization studies. "We're right at the front in thinking ... in recent months that have explored the issue of hybridization in butterflies. ...

Study finds DNA barcoding requires caution without closer examination

... Petota is a complex group because, among other things, there is much hybridization among species; some of the species have multiple divergent copies of their DNA arising from past hybridization (allopolyploidy); there is a mixture of sexual and asexual reproduction; ...

Reducing CO2 through technology and smart growth

... cities by 2050 would have a greater impact on CO2 reductions than full hybridization of the vehicle fleet. Stone's study looked at 11 major metropolitan ... can achieve 40 miles to the gallon and higher. However, even the full hybridization of the national vehicle fleet by 2050 would not meet the CO2 targets ...

Einstein researchers develop technique to count messages made by single genes

... Ph.D., co-chair and professor of anatomy and structural biology at Einstein. The new technology is a powerful refinement of fluorescent in-situ hybridization (FISH), developed in Dr. Singer's laboratory more than 26 years ago. FISH is now a widely used research tool to study gene activation; that is how ...

Plants grow bigger and more vigorously through changes in their internal clocks

... Until now, the molecular mechanisms for hybrid and polyploid vigor have largely been unknown. "Before this discovery, no one really knew how hybridization and polyploidy led to increased vigor," says lead author Dr. Jeffrey Chen, the D. J. Sibley Centennial Professor of Plant Molecular Genetics. "This ...

Growing fuel and medicine: Advancing biofuels and plant-produced therapeutics

... suggest that enzyme amount is affected by several factors including subcellular localization and the promoter used to drive gene expression. Southern hybridization analysis indicates that the gene is present at one copy per genome, simplifying breeding and regulatory issues. Protein accumulation can be increased ...

Tahitian vanilla originated in Maya forests, says UC Riverside botanist

... genes quite frequently across species barriers," Kim said. "This provides an opportunity to breed new commercial varieties of vanilla through hybridization in the future." Lubinsky, Kim and their colleagues plan to do further research on vanilla. In January 2009, they will begin mapping cacao-vanilla ...

New electrostatic-based DNA microarray technique could revolutionize medical diagnostics

... untrue, as our results have plainly demonstrated," said Groves. "We've shown that changes in surface charge density as a result of specific DNA hybridization can be detected and quantified with 50-picometer sensitivity, single base-pair mismatch selectivity, and in the presence of complex backgrounds. ...

When the butterfly bush blossoms

... able to flourish, cover forest floors completely and consequently become a problem. "Its success obviously lies in cultivation. Through selection and hybridization it came to a genetic change, enabling the Oregon grape to grow larger in Europe than in its American homeland ", Dr. Harald Auge of the UFZ explains ...

DNA 'barcode' identified for plants

... every plant species on Earth. In a few groups of species, additional genetic information may be required for species-level identification because hybridization - where species cross-breed and genetic material is rearranged - may confuse the information provided by matK. This research was funded by the ...

Biophysical Society announces winners of 2008 Minority Travel Awards

... Proximal Ligand Switching Mechanism of iNOS. Kumar Sinniah, Calvin College, The Effect of Divalent Cations (Mg2+, Zn2+, Mn2+, and Ca2+) on the hybridization Efficiency of DNA. Danyell Wilson, University of Florida, Single Channel Analysis of a Mechanosensitive Channel Reconstituted in a Tethered ...

Genes from the father facilitate the formation of new species

... research team from Uppsala University in Sweden, working with scientists from Norway, the Czech Republic, the US, and Holland, have studied natural hybridization between two closely related bird species, the collared flycatcher and the pied flycatcher. The two flycatcher species (or quasi species) have come ...

Thriving hybrid salamanders contradict common wisdom

... their competitors and their prey. Such questions will arise more frequently, Shaffer said, as humans both create new opportunities for hybridization with introduced species, and improve the genetic analyses that detect them. ...

Sunflower speciation highlights roles for transposable elements in evolution

... a finding that furthers our understanding of how hybridization may contribute to genome changes and the ... origin of new species through inter-species hybridization may be facilitated by rapid reorganization of ... while the third is adapted to salt marshes. Both hybridization and abiotic stress have been implicated as ...

Study provides new understanding of spontaneous hybridization

... Plant and animal breeders have long used hybridization to transfer useful traits between species. But ... (Indiana University), explore how spontaneous hybridization ?known as adaptive trait introgression ?has a ... and evolutionary diversification. "The role of hybridization in adaptive evolution is contentious. While many ...

Detection of DNA on nanotubes offers new sensing, sequencing technologies

... the optical detection of selective DNA hybridization on the surface of a nanotube," said Strano, who ... a specific sequence of nucleotide bases as rungs. hybridization refers to the spontaneous binding of two ... detection of specific DNA sequences through hybridization with a complementary DNA probe has many potential ...

Study suggests that publicly available genome data may contain small but significant errors

... of Cell Biology at NYU's School of Medicine. Using their micro-array hybridization method, which used flourescently labeled snippets from the genome of the ... The authors show that with appropriate experimental conditions, array hybridization data can be used to establish a physical distance between unique arrayed ...

Breakthrough Microarray-based Technology for the Study of Cancer

... Chromosomal changes in cancer can be studied using Comparative genomic hybridization (CGH), which undergoes dramatic chromosomal changes, including ... among the cancer research community." In the past, comparative genomic hybridization was performed through the optical imaging of whole chromosomes, a ...

Whole genome fine map of rice completed

... The goal of the project was to reveal the molecular mechanism of the hybridization advantages, thus providing the theoretical basis for improving output and ... and the changes of physiological traits, thus revealing the source of the hybridization advantage in hybrid rice. ...

Tibetan antelope slowly recovering, WCS says

... The team also counted more than 1,000 wild yak, a relatively high number for a species that's far more endangered than the chiru, due to hunting and hybridization with domestic yak. The group saw an increase in wild asses, too, though they are persecuted by nomads who believe they compete with livestock for ...

Rapid, Low-Cost DNA Testing

... how Professor Rothberg's procedure is done: Step 1. Hybridization. This takes 10 seconds and costs $0.025. Step 2. Add gold colloid to the hybridization solution. This takes 10 seconds and costs $0.02. Step 3. Add salt to the solution. This takes 10 seconds and costs $0.01. Step 4. Measure ...

Faster, low cost sequencing technologies needed to drive era of personalized medicine

... has hybridized to the probes. Wherever sample DNA binds to the probes, the sequence is registered. "Traditional approaches to sequencing by hybridization are limited by the number of probes that can be placed on a chip," said Jian Gu, a research staff member in the Center for Applied NanoBioscience at ...

NHGRI aims to make DNA sequencing faster, more cost effective

... nano-sized features, a DNA nanoarray can also improve throughput by offering the ability to accommodate billions of DNA molecules in a small area. hybridization will be detected by atomic force microscopy. Carlos H. Mastrangelo, Ph.D., Case Western Reserve University, Cleveland,$815,000 (3 ...

An advanced genetic diagnostic method for multiple myeloma

... of associations between specific chromosomal changes, and thus the description of a new classification of the disease. In addition, the technique of hybridization in situ with fluorescence allowed him to identify new recurrent genetic changes that are involved in the appearance of this pathology. A disease ...

New technology used to construct the first map of structural variation in the human genome

... of the human genome and substantially improves resolution over previous arrays. The array was employed in a process known as comparative genomic hybridization (CGH), which involves tagging genomic DNA from two individuals and then co-hybridizing it to the array. Data from the array were assessed with a new ...

New data from NIH lab confirms protocol to reverse type 1 diabetes in mice

... in the 2003 study, is confirmed in the current work. NIH researchers used cell lineage tracking in the form of Y-chromosomal fluorescence in situ hybridization (FISH), in combination with insulin staining, to follow the fate of male spleen cells transplanted into female recipients. The female mice that ...

Ring-like formations in drying DNA drops could affect hybridization studies

... of the stain, these effects should be accounted for in the design of arrays in which DNA droplets are sequentially deposited onto a glass surface for hybridization studies, the researchers report. "Without optimization of the wetting conditions, it is possible to miss all the DNA in the ring stain of a dried ...

Making a face: A new and earlier marker of neural crest development

... in the journal Nature. The collaborators at Yale and Caltech demonstrate with three different technologies -- immunostaining of proteins, in situ hybridization and multiplex RT-PCR of mRNAs -- that formation of neural crest cells in chick embryos is independent of both mesoderm and neural tissues. They also ...

Neural stem cell gene plays crucial role in eye development

... Cancer Center; Dr. Scott T. Magness, a postdoctoral scientist; B. Matthew Fagan, research technician; Dr.Yongquin Wu, director of the In Situ hybridization Core Facility at the UNC Neuroscience Center; and Scott R. Hutton and Natalie Surzenko, graduate research assistants in the UNC Neurobiology ...

Pathway toward gene silencing described in plants

... at the University of California, Los Angeles. Using mutants, antibodies, and fluorescence microscopy techniques known as RNA fluorescence in situ hybridization (RNA-FISH) and DNA-FISH, Washington University postdoctoral researcher Olga Pontes, Ph.D., was able to unravel where the eight team players are ...

New evidence supports century-old theory of cancer spread

... A Yale School of Medicine study in the December issue of Lancet Oncology challenges mainstream oncology researchers to consider tumor cell hybridization with white blood cells as a major reason that cancer metastasizes or spreads to other parts of the body. "Cancer cells exhibit a remarkable number ...

Divergent mating systems and parental conflict as a barrier to hybridization in flowering plants

... both pre- and post-zygotic consequences of this imbalanced conflict can perturb successful fertilization and development, they provide barriers to hybridization and may facilitate speciation. ...

Motor transport in bio-nano systems

... an instructive example: When integrated into existing microarrays for DNA and RNA hybridization, these transport systems would act to increase the hybridization rates. In general, biomimetic systems based on molecular motors and filaments have many potential applications in bionanotechnology, pharmacology, ...

Agilent Technologies new genome analysis technology set to accelerate Australia fight against mesothelioma

... difficult, and patients are often not identified until the condition is quite advanced. Agilent's new microarray techniques in comparative genomic hybridization (CGH) will allow researchers to rapidly and reliably identify genetic changes in tumorous cells. It is believed that specific genetic changes may ...

Researchers rein in regulatory RNAs

... Upon close examination of several SAT pairs, Abe's group found that the transcripts shared several striking characteristics. Northern blot hybridization analyses of six randomly chosen SAT pairs revealed that the SAT loci generated multiple transcripts of various sizes, in contrast to a single ...
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