template

Variety

...ed to synthesize DNA strand s by actin g as a template ... Full article >>> hnRNA : Heterogeneous nuclear RNA ; refers collectively to the variety of RNA s found in the nucleus , including primary transcript s, partially processed RNA s and snRNA . The term hnRNA is often used ...

Uracil

...osite (complimentary) to the bases in the DNA template strand ; it resembles the sense strand of DNA except that the base thymine is replaced by uracil . Full article >>> A single- strand ed nucleic acid similar to DNA but having ribose sugar rather than deoxyribose sugar ...

Translation

...; translation The synthesis of protein on a template of messenger RNA ; consists of three steps: initiation, elongation, and termination. Making of a polypeptide sequence by trans lating the genetic code of an m RNA molecule associated with a ribosome . Full article >>> Tra...

Transfer RNA

...ed to synthesize DNA strand s by actin g as a template ... Full article >>> There, bound ...> Each gene is trans cribed into a short template molecule of the related polymer RNA , which is better suited for protein synthesis. This in tu...

Transcription

...ranscription the synthesis or RNA from a DNA template ; in retrovirus es, it is the synthesis of DNA from an RNA template ; can also include the synthesis of DNA from a DNA template ( replication ) ... Full article ...

Transcriptase

...at catalyzes the synthesis of DNA from an RNA template . Transcription ... Full article >>>...ncoded by some RNA virus es that uses RNA as a template for DNA synthesis. Full article >>> Reverse transcriptase An enzyme produce...

Transcript

...anscript ion the synthesis or RNA from a DNA template ; in retrovirus es, it is the synthesis of DNA from an RNA template ; can also include the synthesis of DNA from a DNA template ( replication ) ... Full article ...

Trans-

...ncoded by some RNA virus es that uses RNA as a template for DNA synthesis. rhizoid ... Full article >>> In the absence of NPC1, unesterified cholesterol accumulates in the late endosome compartment, which, in turn, disrupts sorting between the endosome and trans- Golgi network (Higgin...

Terminator region

...at attaches to the promoter region of the DNA template , joins nucleotide s to form the synthesized strand of RNA and detaches from the template when it reaches the terminator region . PICTURE ... Full article >>> (See Codon .)...

Template

...eplication each strand of the duplex acts as a template for the synthesis of a new double helix . (3) A...ngle strand ed DNA molecule can be used as a template to synthesise the complement ary nucleotide strand . Related Terms: Deoxyribonucleic acid ( D...

Telomerase

... includes a molecule of RNA that serves as a template for new telomere segment s. telomere ... Full article >>> Telomerase is usually found in cells involved in the production of gametes . It is not normally found in somatic cells . Cyclin-Dependent Kinase s Some cells stop...

T cell

...rulent phage , which destroys the host cell . template A macromolecular pattern for the synthesis of another molecule . For example, DNA is a template for RNA synthesis. Full article >>> Artificial chromosome A vector constructed...

Substrate

...rged ion. c DNA . DNA synthesized from an RNA template using reverse transcriptase . cDNA library . A ...rged ion. c DNA . DNA synthesized from an RNA template using reverse transcriptase . Full article >>> Usually these are small plant s (le...

Strand

...d or partner strand that is complement ary to template for RNA transcription . Related ... Full arti...immortal'; that is, it will serve as an unchanged template as it is passed on from generation to generation [Link]. Full article >>> One stran...

Southern blotting

...probe s representing the sequence s found in the template DNA . The labeled probe s can then be used to i...etc. RT-PCR PCR amplification from an RNA template . Full article >>> Southern blotting -- a technique for trans ferring electrophore...

Small nuclear RNA

...RNA polymerase . Trans cribes RNA from a DNA template . (See Polymerase , RNA .) r RNA . See Ribosomal RNA . S Salmonella. Full article >>> small nuclear RNA ( snRNA ) Any of several small RNA molecule s in the nucleus . Most known snRNA s have a role in the splicing reactions...

SiRNA

...lyte is put onto the membrane using a slot-shaped template . Full article >>> Some RNA genes code for transcript s that function as regulatory RNA s (see siRNA ) that influence the function of other RNA molecule s. Full article >>> Short pieces of double- strand ed RN...

Shotgun cloning

...hat carry smaller insert s and that can serve as template s in sequencing reactions. Full article >>>...

Sense strand

...d of the DNA double helix that serves as the template for m RNA synthesis. Related Terms: Strand ...... AKA: anti sense strand , non-coding strand , template strand The DNA strand that forms the template for both the trans cribed m RNA and the cod...

Sense

... ed DNA . The anti sense strand serves as the template for m RNA synthesis. explained: Listen to a detailed explanation. Full article >>> Sense of balance, which maintains physical balance in human s and animals In the physical sciences ... Full article >>> Sense s...

Semi-conservative

...rand of DNA remains intact as it serves as the template for the assembly of a complement ary strand . Full article >>>...

Semen

...A helix is unwound and each strand serves as a template for the synthesis of a new complement ary strand , ... Full article >>> semen removed from the vagina of the rape victim (EVIDENCE #2); a semen stain left on the victim's clothing (EVIDENCE #1); the DNA of the victim herse...

Segment

...t of a segment of chromosomal DNA which is a template for protein synthesis. Methyl trans ferase Enzymes that catalyze the trans fer of methyl groups from one molecule to another. Full article >>> A ‘ contig ' may refer to a map showing placement of a set of clones that co...

Run-on

...of an RNA transcript lies with respect to its template DNA (the gene ). Can't be described in a short paragraph. Full article >>> Nuclear run-on : A method used to estimate the relative rate of transcription of a given gene , as opposed to the steady-state level of the m RNA tran...

RT-PCR

...of an RNA transcript lies with respect to its template DNA (the gene ). Can't be described in a short paragraph. Full article >>> RT-PCR See Reverse Transcription - PCR ( RT-PCR ) Recombinant Recombinant DNA (r DNA ) Recombination frequency Replication fork Response el...

RNA Splicing

...rom NTPs, using a strand of DNA or RNA as a template . RNA splicing Removal of introns and joining of exons in a primary transcript ( hnRNA ). Full article >>>...

RNA polymerase

... of nucleic acids on preexisting nucleic acid template s, assembling RNA from ribonucleotides . Relate...be trans cribed. Only one strand of DNA (the template strand ) is trans cribed. Full article >>> RNA polymerase ( RNA P) An enzyme ...

RNA

... of DNA (the anti-sense strand ) is used as a template to produce a single strand of m RNA . Full art...be trans cribed. Only one strand of DNA (the template strand ) is trans cribed. Full article >>> RNA splicing - RNA - processing s...

Ribozyme

...rged ion. c DNA . DNA synthesized from an RNA template using reverse transcriptase . cDNA library . Full article >>> Ribozyme s Information to link the RNA world to the DNA and RNA world. A summary of ribozyme s by Access Excellence. The RNA World (IMB Jena, Germany) Links to...

Ribosome

... - protein that synthesizes RNA from a DNA template . Back to the Top ... Full article >>> RIBOSOME - A complex ribonucleo protein particle (eukaryotic ribosome s contain 4 RNA s and at least 82 proteins ) which is the "machine" which trans lates m RNA into protein molecule...

Ribosomal RNA

... - protein that synthesizes RNA from a DNA template . Back to the Top ... Full article >>> This chain of events involving RNA transcription , and polypeptide translation is referred to as gene expression . Some genes encode other elements such as ribosomal RNA s and transfer...

Ribonucleotides

...cleotides (NTPs), using one strand of DNA as template (hence called " DNA -dependent RNA polymerase ).... of nucleic acids on preexisting nucleic acid template s, assembling RNA from ribonucleotides or DNA from deoxyribonucleotide s. A B C D E F G H I J...

Reverse transcriptase

...at catalyzes the synthesis of DNA from an RNA template . Transcription ... Full article >>>...ncoded by some RNA virus es that uses RNA as a template for DNA synthesis. Full article >>> Reverse transcriptase An enzyme produced ...

Retrovirus

...iral RNA . The new DNA produced from the RNA template is called c DNA . Full article >>> ...ize a strand of DNA complimentary to an RNA template . ( Reverse transcriptase can also synthesize DNA from a DNA template .) Used to make cDNA cl...

Restriction site

... enzyme which will make a DNA copy of an RNA template - a DNA -dependant RNA polymerase . Full article >>> Restriction site : See Restriction enzyme . Restriction fragment length polymorphism ( RFLP ): Variation in the distance between restriction enzyme cleavage sites that...

Replication fork

...s the end of the DNA , there is no longer enough template to continue forming Okazaki fragments . So the 5' end of each newly-synthesized strand cannot be completed. Full article >>> This origin of replication is unwound, and the partially unwound strand s form a " replication bubble "...

Replication

...NA replication : The use of existing DNA as a template for the synthesis of new DNA strand s. DNA sequence : The relative order of base pair s in any sample of DNA . See base sequence analysis . Full article >>> DNA replication : the process by which cells replicate or synth...

Recessive

...DNA -- complementary DNA produced from a RNA template by the action of RNA - dependent DNA polymerase . Full article >>> Mutation s in the AE1 gene cause autosomal dominant and recessive forms of dRTA. All the dominant dRTA mutation s investigated cause aberrant trafficking...

Recessive gene

...DNA -- complementary DNA produced from a RNA template by the action of RNA - dependent DNA polymerase . Full article >>> Where an individual receives one albinism gene and one normal gene , that person will not show outward signs of the condition, but will become a carrier of ...

Promoter

... it and uses it to begin trans cribing the DNA template into a new RNA . Full article >>> Promoter . A region of DNA extending 150-300 bp upstream from the transcription start site that contains binding site s for RNA polymerase and a number of proteins that regulate the rate ...

Primer extension

...hich DNA is reverse trans cribed from an RNA template to which a specific oligonucleotide primer has been anneal ed. Full article >>> Primer extension : This is a method used to figure out how far upstream from a fixed site the start of an m RNA is. For example, perhaps you ha...

Primer

...DNA polymerase . It anneal s to a nucleic acid template and promotes copying of the template starting from the primer site . Full article >>> Primer pheromones are detected...

Primase

...e . Primase does not require a primer , only a template . Full article >>> The complex of T7 DNA polymerase and T7 helicase / primase synthesize DNA with high efficiency. We have been optimizing reactions carried out by these enzymes in combination with other T7 replication prote...

Polymerase

...slipping of DNA polymerase III from the DNA template strand at the repeat region and the subsequent ... of nucleic acids on preexisting nucleic acid template s, assembling DNA from deoxyribonucleotide s. Cf. RNA polymerase . Related Terms: Enzyme ......

PCR

...): Single- strand ed DNA produced from an RNA template (usually m RNA ) by reverse transcriptase in vitro. It lacks the introns present in corresponding genomic DNA . It is most commonly made to use in PCR to amplify RNA ( RT - PCR ). Full article >>> PCR -- polymerase chain...

Palindromic Sequence

... to the dissociation of the RNA P from the DNA template .One such common termination motif is the palindromic sequence 'GCCGCCAG'. Full article >>> Many DNA -binding proteins recognize palindromic sequence s. Note that the grammatical usage of this term is differnet than the way it ...

Nucleoli

...is a fundamental component since it serves as the template for transcription of the ribosomal RNA (r RNA ) for inclusion into new ribosomes . Full article >>> Nucleoli are the sites of ribosomal RNA (r RNA ) synthesis. The large and small r RNA s are derived from a single transcript...

Nucleic acids

... of nucleic acids on preexisting nucleic acid template s, assembling DNA from deoxyribonucleotide s. C...at catalyzes the synthesis of DNA from an RNA template . Full article >>> Large polynucleotides can either form ribonucleic acid s ( RNA ) ...

Non-coding strand

...nd ed DNA . The antisense strand serves as the template for m RNA synthesis. explained: Listen to a deta...; AKA: antisense strand , non-coding strand , template strand The DNA strand that forms the template for both the trans cribed m RNA and the cod...

Molecule

...ngle strand ed DNA molecule can be used as a template to synthesise the complement ary nucleotide s... DNA molecule using a primer and DNA as a template . (See Poly(A) polymerase , Polymerase chain re action , RNA polymerase , Taq polymerase .) ......

Minerals

...complementary DNA strand synthesized along the template strand in the mandatory 5' to 3' direction. Full article >>> While movement of water and minerals through the xylem is driven by negative pressures (tension) most of the time, movement through the phloem is driven by positive ...

Messenger RNA

... Messenger RNA (m RNA ) RNA that serves as a template for protein synthesis or for synthesis of c DNA... Messenger RNA (m RNA ) RNA that serves as a template for protein synthesis. See also: genetic code Metaphase A stage in mitosis or meiosis du...

Mapping

...of an RNA transcript lies with respect to its template DNA (the gene ). Can't be described in a short paragraph. See " RNAse Protection assay" for a closely related technique. Full article >>> -Concept Mapping -Visual discrimination of physical/chemical character istics Rocks and M...

Lysis

...lyte is put onto the membrane using a slot-shaped template . The template produces a consistently shaped spot, thus decreasing errors and improving the accuracy of the ana l...

Ligase

...ther. DNA polymerase An enzyme that catalyzes template -dependent synthesis of DNA from its dNTP precursors. Full article >>>...

Leading strand

...complementary DNA strand synthesized along the template strand in the mandatory 5' to 3' direction. le...along it in the 3' to 5' direction, using it as a template for assembling a leading strand of nucleotide s and reforming a double helix . Full article &#...

Klenow Fragment

...' -> 3' fashion (addition of nucleotide s to template d free 3' ends of primer s). Full article >>> In contrast, two other polymerase s used for DNA sequencing , Klenow fragment of E. coli DNA polymerase I and Taq DNA polymerase , discriminate against dideoxynucleotides up to one ...

Inverted repeat

...ith repetitious thymidine residue s in the DNA template . or where a GC-rich inverted repeat followed by 4 A residue s. Full article >>> A general term for any genetic unit that can insert into a chromosome , exit, and relocate; includes insertion sequences , transposon s, some bac...

Insertion mutations

...d for ana lysis of gene expression with m RNA template s and reverse transcriptase . Substitution , deletion , and insertion mutations have been detected. Full article >>>...

In vitro

...): Single- strand ed DNA produced from an RNA template (usually m RNA ) by reverse transcriptase in vitro. It lacks the introns present in corresponding genomic DNA . It is most commonly made to use in PCR to amplify RNA ( RT - PCR ). Full article >>> Enzyme that cata lysis t...